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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1388R-1 |
| Symbol |
Tak1 |
Full Name |
TGF-beta activated kinase 1 |
| CG No |
CG18492 |
Old CG No |
CG1388 |
| Synonyms |
dTAK1, DTAK1, TAK, D-tak, dTak1, TAK-1, dTAK, tak1, TAK1, tak, D10, DmTak1, CG18492, CG1388, DTak1, DTak, Tak 1, Tak1 |
| Accession No (Link to NCBI) |
NM_079356.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Neisch AL, Speck O, Stronach B, Fehon RG.
Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
J Cell Biol (2010) 189(2) 311-23
[
PubMed ID = 20404112
]
[
RRC reference
]
Bangi E, Pitsouli C, Rahme LG, Cagan R, Apidianakis Y.
Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells.
EMBO Rep (2012) 13(6) 569-76
[
PubMed ID = 22498775
]
[
RRC reference
]
Nelson B, Freisinger T, Ishii K, Okado K, Shinzawa N, Fukumoto S, Kanuka H.
Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila.
Biochem Biophys Res Commun (2013) 430(3) 1120-5
[
PubMed ID = 23261474
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Leulier F, Vidal S, Saigo K, Ueda R, Lemaitre B.
Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults.
Curr Biol (2002) 12(12) 996-1000
[
PubMed ID = 12123572
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGCCACAGCATCGCTGGAC |
| Primer Seq. 3' |
aatctagaggtaccCCACCGTGCCGAAGTCGCATA |
| Predicted Fragment Size |
490 |
| IR fragment full Seq |
0001 ATGGCCACAG CATCNCTGGA CGCACTGCAG GCAGCCTATG TGGACTTCAG TGAGATAACA
0061 CTAAGAGAGA AAGTCGGCCA TGGGTCCTAC GGTGTGGTCT GCAAGGCCGT TTGGCGCGAC
0121 AAGCTGGTTG CCGTCAAGGA GTTCTTCGCC AGCGCCGAGC AGNAAGGACA TCGAGAAGGA
0181 GGTGAAGCAG TTGTCGCGCG TGAAGCACCC GAACATCATC GCTCTGCACG GGATATCCTC
0241 GTACCAGCAG GCCACCTACC TGATAATGGA GTTCGCCGAA GGTGGATCGC TGCACAACTT
0301 NCCTTCACGG CAAGGTGAAG CCGGCATATT CTNCTGGCCC ACGCCATGAG CTGGGCGCGC
0361 CAATGTGCAG AGGGTCTGGC ATATTTGCAT GCCATGACGC CAAAACCACT AATACATCGC
0421 GACGTGAAGC CGCTGAACCT GCTCTTGACC AACAAGGGAC GCAATCTGAA GATATGCGAC
0481 TTCGGCACGG TGG |
| in silico PCR Fragment |
0001 ATGGCCACAG CATCGCTGGA CGCACTGCAG GCAGCCTATG TGGACTTCAG TGAGATAACA
0061 CTAAGAGAGA AAGTCGGCCA TGGGTCCTAC GGAGTGGTCT GCAAGGCCGT TTGGCGCGAC
0121 AAGCTGGTTG CCGTCAAGGA GTTCTTCGCC AGCGCCGAGC AG-AAGGACA TCGAGAAGGA
0181 GGTGAAGCAG TTGTCGCGCG TGAAGCACCC GAACATCATC GCTCTGCACG GGATATCCTC
0241 GTACCAGCAG GCCACCTACC TGATAATGGA GTTCGCCGAA GGTGGATCGC TGCACAACTT
0301 -CCTTCACGG CAAGGTGAAG CCGGCATATT CT-CTGGCCC ACGCCATGAG CTGGGCGCGC
0361 CAATGTGCAG AGGGTCTGGC ATATTTGCAT GCCATGACGC CAAAACCACT AATACATCGC
0421 GACGTGAAGC CGCTGAACCT GCTCTTGACC AACAAGGGAC GCAATCTGAA GATATGCGAC
0481 TTCGGCACGG TGG |
| Assemble Data |
1388R-1.IR_full 1 ATGGCCACAGCATCNCTGGACGCACTGCAGGCAGCCTATGTGGACTTCAGTGAGATAACA 60
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
1388R-1.in silico 1 ATGGCCACAGCATCGCTGGACGCACTGCAGGCAGCCTATGTGGACTTCAGTGAGATAACA 60
1388R-1.IR_full 61 CTAAGAGAGAAAGTCGGCCATGGGTCCTACGGTGTGGTCTGCAAGGCCGTTTGGCGCGAC 120
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
1388R-1.in silico 61 CTAAGAGAGAAAGTCGGCCATGGGTCCTACGGAGTGGTCTGCAAGGCCGTTTGGCGCGAC 120
1388R-1.IR_full 121 AAGCTGGTTGCCGTCAAGGAGTTCTTCGCCAGCGCCGAGCAGNAAGGACATCGAGAAGGA 180
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
1388R-1.in silico 121 AAGCTGGTTGCCGTCAAGGAGTTCTTCGCCAGCGCCGAGCAG-AAGGACATCGAGAAGGA 180
1388R-1.IR_full 181 GGTGAAGCAGTTGTCGCGCGTGAAGCACCCGAACATCATCGCTCTGCACGGGATATCCTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1388R-1.in silico 181 GGTGAAGCAGTTGTCGCGCGTGAAGCACCCGAACATCATCGCTCTGCACGGGATATCCTC 240
1388R-1.IR_full 241 GTACCAGCAGGCCACCTACCTGATAATGGAGTTCGCCGAAGGTGGATCGCTGCACAACTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1388R-1.in silico 241 GTACCAGCAGGCCACCTACCTGATAATGGAGTTCGCCGAAGGTGGATCGCTGCACAACTT 300
1388R-1.IR_full 301 CCTTCACGGCAAGGTGAAGCCGGCATATTCTNCTGGCCCACGCCATGAGCTGGGCGCGC 359
||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
1388R-1.in silico 301 CCTTCACGGCAAGGTGAAGCCGGCATATTCT-CTGGCCCACGCCATGAGCTGGGCGCGC 359
1388R-1.IR_full 361 CAATGTGCAGAGGGTCTGGCATATTTGCATGCCATGACGCCAAAACCACTAATACATCGC 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1388R-1.in silico 361 CAATGTGCAGAGGGTCTGGCATATTTGCATGCCATGACGCCAAAACCACTAATACATCGC 419
1388R-1.IR_full 421 GACGTGAAGCCGCTGAACCTGCTCTTGACCAACAAGGGACGCAATCTGAAGATATGCGAC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1388R-1.in silico 421 GACGTGAAGCCGCTGAACCTGCTCTTGACCAACAAGGGACGCAATCTGAAGATATGCGAC 479
1388R-1.IR_full 481 TTCGGCACGGTGG 492
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1388R-1.in silico 481 TTCGGCACGGTGG 492
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
472 |
0 |
0 |
0 |
NM_079356.2 |
CG18492-RA (Tak1), mRNA |
| 0 |
0 |
4 |
3 |
5 |
NM_167080.1 |
CG32742-RA (l(1)G0148), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_132629.1 |
CG4330-RA (CG4330), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_079454.2 |
CG6948-RA (Clc), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_167705.1 |
CG1695-RB, transcript variant B (CG1695), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_134550.1 |
CG1695-RA, transcript variant A (CG1695), mRNA |
| 0 |
0 |
2 |
4 |
10 |
NM_134551.4 |
CG32506-RA (CG32506), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_057303.4 |
CG7831-RA (ncd), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169546.1 |
CG9374-RH, transcript variant H (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169542.1 |
CG9374-RC, transcript variant C (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169547.1 |
CG9374-RI, transcript variant I (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169545.1 |
CG9374-RF, transcript variant F (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_142045.2 |
CG9374-RB, transcript variant B (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169543.1 |
CG9374-RD, transcript variant D (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169544.1 |
CG9374-RE, transcript variant E (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169541.1 |
CG9374-RA, transcript variant A (lkb1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132320.2 |
CG12124-RA (CG12124), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136533.1 |
CG11641-RA (pdm3), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_166148.1 |
CG8430-RB, transcript variant B (Got1), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_137242.2 |
CG8430-RA, transcript variant A (Got1), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_135937.2 |
CG4930-RA (CG4930), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141795.2 |
CG6693-RA (CG6693), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166045.1 |
CG6693-RA (CG6693), mRNA,water dikinase CG8553-RA, transcript variant A (SelD), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166046.1 |
CG6693-RA (CG6693), mRNA,water dikinase CG8553-RA, transcript variant A (SelD), mRNA,water dikinase CG8553-RB, transcript variant B (SelD), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_080308.2 |
CG2845-RA, transcript variant A (phl), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001042793.1 |
CG2845-RB, transcript variant B (phl), mRNA |
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