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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13746R-2

Symbol MrgBP Full Name MrgBP

CG No CG13746 Old CG No CG13746

Synonyms dMrgB, dMrgBP, CG13746, MrgBP

Accession No (Link to NCBI) NM_136573.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55 [ PubMed ID = 25117438 ] [ RRC reference ]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCAAGGACAAGGGTCTGGCT

Primer Seq. 3'

aatctagaggtaccCCGGCGTTTTGGTTGCAGGTG

Predicted Fragment Size

IR fragment full Seq

0001 GCCAAGAGAC AAGGGTCTGG CTGTGAGNAG CCGGAAcgGC GGCCCCCTTA CCGGCCGCCC 
0061 TCGACCACGA GTGGTCCGCC GAGGAAGAGC TGCAGCTGTT CCATGCAATG GAAGGCCTGC 
0121 GCCCGGTGGG CATCAACAAG CACTTCTACA TGTCCTGCAT TGTCCAGCGT CTGTCCAAGT 
0181 CGCTGAACCG TGAAATGCCC AGTGAGCTGA TCTGGCGCCA CCTGGGCACC ATGTACAAGC 
0241 TGAAGGAACT GGACGATCTG GAGTCGCTGC CCTTTCCCAA CGAGGAGCGC GAGTTCAGCC 
0301 TGCCGGAGCA GGATTACGGA ACGCTACAGA CCAAAAAGAC GGTGGAAGTG CATGCCAACG 
0361 AGGAGGCTGC AGAGGCACAG TCGGCCCCTC CAACAGCAGA GACCAATGGC AAAGCTCCAC 
0421 CTGCAACCAA AACGCCGG

in silico PCR Fragment

0001 GCCAAG-GAC AAGGGTCTGG CTGTG----G CCGGAACGGC GGCCCCCTTA CCGGCCGCCC 
0061 TCGACCACGA GTGGTCCGCC GAGGAAGAGC TGCAGCTGTT CCATGCAATG GAAGGCCTGC 
0121 GCCCGGTGGG CATCAACAAG CACTTCTACA TGTCCTGCAT TGTCCAGCGT CTGTCCAAGT 
0181 CGCTGAACCG TGAAATGCCC AGCGAGCTGA TCTGGCGCCA CCTGGGCACC ATGTACAAGC 
0241 TGAAGGAACT GGACGATCTG GAGTCGCTGC CCTTTCCCAA CGAGGAGCGC GAGTTCAGCC 
0301 TGCCGGAGCA GGATTACGGA ACGCTACAGA CCAAAAAGAC GGTGGAAGTG CATGCCAACG 
0361 AGGAGGCTGC AGAGGCACAG TCGGCCCCTC CAACAGCAGA GACCAATGGC AAAGCTCCAC 
0421 CTGCAACCAA AACGCCGG

Assemble Data


13746R-2.IR_full       1   GCCAAGAGACAAGGGTCTGGCTGTGAGNAGCCGGAACGGCGGCCCCCTTACCGGCCGCCC 60
                           |||||| ||||||||||||||||||    |||||||||||||||||||||||||||||||
13746R-2.in silico     1   GCCAAG-GACAAGGGTCTGGCTGTG----GCCGGAACGGCGGCCCCCTTACCGGCCGCCC 60


13746R-2.IR_full       61  TCGACCACGAGTGGTCCGCCGAGGAAGAGCTGCAGCTGTTCCATGCAATGGAAGGCCTGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13746R-2.in silico     61  TCGACCACGAGTGGTCCGCCGAGGAAGAGCTGCAGCTGTTCCATGCAATGGAAGGCCTGC 120


13746R-2.IR_full       121 GCCCGGTGGGCATCAACAAGCACTTCTACATGTCCTGCATTGTCCAGCGTCTGTCCAAGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13746R-2.in silico     121 GCCCGGTGGGCATCAACAAGCACTTCTACATGTCCTGCATTGTCCAGCGTCTGTCCAAGT 180


13746R-2.IR_full       181 CGCTGAACCGTGAAATGCCCAGTGAGCTGATCTGGCGCCACCTGGGCACCATGTACAAGC 240
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
13746R-2.in silico     181 CGCTGAACCGTGAAATGCCCAGCGAGCTGATCTGGCGCCACCTGGGCACCATGTACAAGC 240


13746R-2.IR_full       241 TGAAGGAACTGGACGATCTGGAGTCGCTGCCCTTTCCCAACGAGGAGCGCGAGTTCAGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13746R-2.in silico     241 TGAAGGAACTGGACGATCTGGAGTCGCTGCCCTTTCCCAACGAGGAGCGCGAGTTCAGCC 300


13746R-2.IR_full       301 TGCCGGAGCAGGATTACGGAACGCTACAGACCAAAAAGACGGTGGAAGTGCATGCCAACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13746R-2.in silico     301 TGCCGGAGCAGGATTACGGAACGCTACAGACCAAAAAGACGGTGGAAGTGCATGCCAACG 360


13746R-2.IR_full       361 AGGAGGCTGCAGAGGCACAGTCGGCCCCTCCAACAGCAGAGACCAATGGCAAAGCTCCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13746R-2.in silico     361 AGGAGGCTGCAGAGGCACAGTCGGCCCCTCCAACAGCAGAGACCAATGGCAAAGCTCCAC 420


13746R-2.IR_full       421 CTGCAACCAAAACGCCGG 438
                           ||||||||||||||||||
13746R-2.in silico     421 CTGCAACCAAAACGCCGG 438

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	415	0	2	2	NM_136573.2	CG13746-RA (MrgBP), mRNA
0.48	2	2	2	3	NM_167539.1	CG32571-RA (CG32571), mRNA
0	0	3	2	8	NM_079750.3	CG5436-RA (Hsp68), mRNA
0	0	2	3	6	NM_001043261.1	CG34157-RG, transcript variant G (Dys), mRNA
0	0	2	3	6	NM_001043258.1	CG34157-RA, transcript variant A (Dys), mRNA
0	0	2	3	6	NM_001043259.1	CG34157-RC, transcript variant C (Dys), mRNA
0	0	2	3	6	NM_001043257.1	CG34157-RH, transcript variant H (Dys), mRNA
0	0	2	3	6	NM_001043263.1	CG34157-RF, transcript variant F (Dys), mRNA
0	0	2	3	5	NM_135330.1	CG7356-RA, transcript variant A (CG7356), mRNA
0	0	2	3	4	NM_133156.1	CG12202-RA (Nat1), mRNA
0	0	2	3	3	NM_001043256.1	CG34157-RB, transcript variant B (Dys), mRNA
0	0	2	3	3	NM_169632.1	CG6384-RB, transcript variant B (Cp190), mRNA
0	0	2	3	3	NM_079635.2	CG6384-RA, transcript variant A (Cp190), mRNA
0	0	2	3	3	NM_001043260.1	CG34157-RD, transcript variant D (Dys), mRNA
0	0	2	3	3	NM_001043262.1	CG34157-RE, transcript variant E (Dys), mRNA
0	0	2	2	7	NM_140236.2	CG7334-RA, transcript variant A (Sug), mRNA
0	0	2	2	7	NM_206335.1	CG7334-RB, transcript variant B (Sug), mRNA
0	0	2	2	5	NM_164778.1	CG7356-RB, transcript variant B (CG7356), mRNA
0	0	2	2	3	NM_080316.1	CG2655-RA (HLH3B), mRNA
0	0	2	2	2	NM_135305.3	CG7149-RA (CG7149), mRNA
0	0	2	2	2	NM_057546.2	CG3090-RA, transcript variant A (Sox14), mRNA
0	0	2	2	2	NM_134290.1	CG3090-RB, transcript variant B (Sox14), mRNA
0	0	1	5	4	NM_079319.2	CG4153-RA (eIF-2beta), mRNA
0	0	1	4	7	NM_143426.2	CG14508-RA (CG14508), mRNA
0	0	1	4	3	NM_135017.2	CG15628-RA (CG15628), mRNA
0	0	1	4	2	NM_132113.2	CG3184-RA (CG3184), mRNA
0	0	1	2	8	NM_057454.3	CG16720-RA, transcript variant A (5-HT1A), mRNA
0	0	1	2	8	NM_166322.1	CG16720-RB, transcript variant B (5-HT1A), mRNA
0	0	1	2	2	NM_143574.1	CG15546-RA (CG15546), mRNA
0	0	1	1	3	NM_168291.1	CG5939-RC, transcript variant C (Prm), mRNA

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