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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12908R-3

Symbol Ndg Full Name Nidogen/entactin

CG No CG12908 Old CG No CG12908

Synonyms CG12908, ndg, CT32053, BEST:CK00552, Ndg

Accession No (Link to NCBI) NM_136731.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Kim MJ, Choe KM.
Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
PLoS Genet (2014) 10(10) e1004683 [ PubMed ID = 25329560 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGCCGACCTTCGGCAGTAAG

Primer Seq. 3'

aatctagaggtaccAAGTAGCCCACATTGCGCCAC

Predicted Fragment Size

IR fragment full Seq

0001 ATGCCGACCT TCGgcAGTAA GTTGCTCGCC TGCCTGCTGC TCAGCTCGGT GATCCTGGTC 
0061 AGCGGACAGT TCGAGCACTA TCTGGACAGC CTGCACGCCT CGGAATTGTA CGAGTTCGAG 
0121 GATGGCTCCC TGGGCAGCAT ACACCTCTTG CCCAAGGGCG ACAGCGAAAC GATCGTTCTG 
0181 CAACTGGAGC AGCCCATTCA CTTCTACGGG GAGCAGTACG AGCAGCTCTA TATCAACACC 
0241 AATGGCATCC TCACCTTCAA CTCCGAGTTT CCCGAGTACC TGAACCAGCC CTTTCCGCTG 
0301 GAATATGCTT CGATAGCGGC CTTCTACTCG AACGTGGACA CCTCGTTCAG TGATGAGGGC 
0361 ACCTCGATCT CCCTGTTCGA GTCCAAGGAA CAAAGTATTC TCGACAGGGC CTCCAGTTTG 
0421 GTGCGTTACG CCTTTAGCAG CCAGTCTGAA TTCGAGGCTC GTCAGGTGAT CGTGGNCACG 
0481 TGGCGCAATG TGGGCTACTT

in silico PCR Fragment

0001 ATGCCGACCT TCGGCAGTAA GTTGCTCGCC TGCCTGCTGC TCAGCTCGGT GATCCTGGTC 
0061 AGCGGACAGT TCGAGCACTA TCTGGACAGC CTGCGCGCCT CGGAATTGTA CGAGTTCGAG 
0121 GATGGCTCCC TGGGCAGCAT ACACCTCTTG CCCAAGGGCG ACAGCGAAAC GATCGTGCTG 
0181 CAACTGGAGC AGCCCATTCA CTTCTACGGG GAGCAGTACG AGCAGCTCTA TATCAACACC 
0241 AATGGCATCC TCACCTTCAA CTCCGAGTTT CCCGAGTACC TGAACCAGCC CTTTCCGCTG 
0301 GAATATGCTT CGATAGCGGC CTTCTACTCG AACGTGGACA CCTCGTTCAG TGATGAGGGC 
0361 ACCTCGATCT CCCTGTTCGA GTCCAAGGAA CAAAGTATTC TCGACAGGGC CTCCAGTTTG 
0421 GTGCGTTACG CCTTTAGCAG CCAGTCTGAA TTCGAGGCTC GTCAGGTGAT CGTGGCCACG 
0481 TGGCGCAATG TGGGCTACTT

Assemble Data


12908R-3.IR_full       1   ATGCCGACCTTCGGCAGTAAGTTGCTCGCCTGCCTGCTGCTCAGCTCGGTGATCCTGGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12908R-3.in silico     1   ATGCCGACCTTCGGCAGTAAGTTGCTCGCCTGCCTGCTGCTCAGCTCGGTGATCCTGGTC 60


12908R-3.IR_full       61  AGCGGACAGTTCGAGCACTATCTGGACAGCCTGCACGCCTCGGAATTGTACGAGTTCGAG 120
                           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
12908R-3.in silico     61  AGCGGACAGTTCGAGCACTATCTGGACAGCCTGCGCGCCTCGGAATTGTACGAGTTCGAG 120


12908R-3.IR_full       121 GATGGCTCCCTGGGCAGCATACACCTCTTGCCCAAGGGCGACAGCGAAACGATCGTTCTG 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
12908R-3.in silico     121 GATGGCTCCCTGGGCAGCATACACCTCTTGCCCAAGGGCGACAGCGAAACGATCGTGCTG 180


12908R-3.IR_full       181 CAACTGGAGCAGCCCATTCACTTCTACGGGGAGCAGTACGAGCAGCTCTATATCAACACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12908R-3.in silico     181 CAACTGGAGCAGCCCATTCACTTCTACGGGGAGCAGTACGAGCAGCTCTATATCAACACC 240


12908R-3.IR_full       241 AATGGCATCCTCACCTTCAACTCCGAGTTTCCCGAGTACCTGAACCAGCCCTTTCCGCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12908R-3.in silico     241 AATGGCATCCTCACCTTCAACTCCGAGTTTCCCGAGTACCTGAACCAGCCCTTTCCGCTG 300


12908R-3.IR_full       301 GAATATGCTTCGATAGCGGCCTTCTACTCGAACGTGGACACCTCGTTCAGTGATGAGGGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12908R-3.in silico     301 GAATATGCTTCGATAGCGGCCTTCTACTCGAACGTGGACACCTCGTTCAGTGATGAGGGC 360


12908R-3.IR_full       361 ACCTCGATCTCCCTGTTCGAGTCCAAGGAACAAAGTATTCTCGACAGGGCCTCCAGTTTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12908R-3.in silico     361 ACCTCGATCTCCCTGTTCGAGTCCAAGGAACAAAGTATTCTCGACAGGGCCTCCAGTTTG 420


12908R-3.IR_full       421 GTGCGTTACGCCTTTAGCAGCCAGTCTGAATTCGAGGCTCGTCAGGTGATCGTGGNCACG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
12908R-3.in silico     421 GTGCGTTACGCCTTTAGCAGCCAGTCTGAATTCGAGGCTCGTCAGGTGATCGTGGCCACG 480


12908R-3.IR_full       481 TGGCGCAATGTGGGCTACTT 500
                           ||||||||||||||||||||
12908R-3.in silico     481 TGGCGCAATGTGGGCTACTT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_206074.1	CG12908-RB, transcript variant B (Ndg), mRNA
100	482	0	0	0	NM_136731.1	CG12908-RA, transcript variant A (Ndg), mRNA
0	0	3	4	6	NM_166555.1	CG3510-RA, transcript variant A (CycB), mRNA
0	0	3	4	6	NM_166556.1	CG3510-RD, transcript variant D (CycB), mRNA
0	0	3	4	6	NM_166557.1	CG3510-RB, transcript variant B (CycB), mRNA
0	0	3	4	6	NM_166558.1	CG3510-RC, transcript variant C (CycB), mRNA
0	0	2	6	11	NM_206600.1	CG3019-RC, transcript variant C (su(w[a])), mRNA
0	0	2	6	11	NM_206601.1	CG3019-RB, transcript variant B (su(w[a])), mRNA
0	0	2	6	11	NM_057408.3	CG3019-RA, transcript variant A (su(w[a])), mRNA
0	0	2	6	11	NM_001042791.1	CG3019-RF, transcript variant F (su(w[a])), mRNA
0	0	2	4	11	NM_001042792.1	CG3019-RD, transcript variant D (su(w[a])), mRNA
0	0	2	2	5	NM_057242.3	CG7765-RA (Khc), mRNA
0	0	2	2	4	NM_137083.2	CG8233-RB, transcript variant B (CG8233), mRNA
0	0	1	5	5	NM_079693.2	CG4173-RA (Sep2), mRNA
0	0	1	4	11	NM_206286.1	CG10107-RC, transcript variant C (CG10107), mRNA
0	0	1	4	11	NM_139799.3	CG10107-RA, transcript variant A (CG10107), mRNA
0	0	1	4	6	NM_140047.1	CG4461-RA (CG4461), mRNA
0	0	1	4	4	NM_138063.2	CG13578-RA (CG13578), mRNA
0	0	1	3	5	NM_140449.1	CG9592-RA (CG9592), mRNA
0	0	1	3	4	NM_167869.1	CG9184-RA, transcript variant A (CG9184), mRNA
0	0	1	3	4	NM_138255.1	CG9184-RB, transcript variant B (CG9184), mRNA
0	0	1	3	3	NM_136442.3	CG1621-RA (CG1621), mRNA
0	0	1	3	2	NM_137046.3	CG6191-RA (CG6191), mRNA
0	0	1	2	24	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	2	7	NM_079453.2	CG6975-RA (gig), mRNA
0	0	1	2	4	NM_140973.1	CG5955-RA (CG5955), mRNA
0	0	1	2	4	NM_167965.1	CG32296-RA (Mrtf), mRNA
0	0	1	2	4	NM_140067.1	CG3654-RD (CG3654), mRNA
0	0	1	2	3	NM_078795.2	CG18241-RA (Toll-4), mRNA
0	0	1	2	3	NM_144078.1	CG18676-RA (Teh3), mRNA

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