|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12866R-3 |
| Symbol |
CG12866 |
Full Name |
CG12866 |
| CG No |
CG12866 |
Old CG No |
CG12866 |
| Synonyms |
CG12866 |
| Accession No (Link to NCBI) |
NM_137136.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Proc. Natl. Acad. Sci. U.S.A. (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.
Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.
J. Cell Biol. (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCGGCACAGTTTCAACGGATA |
| Primer Seq. 3' |
aatctagaggtaccGGTTTCCTTAGGGACCACATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCGGCACAGT TTCAACGGAT ATCCTGAATG GGCGCACATC ATTCTTGTCC CTTAGTCCAG
0061 TCCTCACGGG AGTGAAGTGG ATTTGCCTNC TTCGTTCTAC GAAAGCTTTA ATCCAACTTT
0121 TCTTCCCAAG CTGCCTATCA GAAGATTTCC CCATATGCTT GTGGGGGCAT CAAATGAGCC
0181 TGAGATTTCG NAAAAACCAG CCAGCAATTC CACCATCTAG GCCAATTGAG CCAATATCTG
0241 AGGTGCCGTT TGTGGAACCG GCCTTTGAGA AAAAATACTC TAGGACCCGT GGATTTAGCA
0301 AGGAATTGTC CACACACAGG GATAATGAGA ATGAATATGT CGAGAGCACA GATGAGGTGT
0361 CTCAGATGTT GAGCTTTACT AGCTATACGG ATGAATATGA TAGGGCAGAA CCACTCATTA
0421 CCAAAACGCA AAAGTTTTAT AAAAAGAGAT CCCCCTTGAT TCCCAAATAC GATTCCGAGG
0481 ATTCATATGT GGTCCCTAAG GAAACC |
| in silico PCR Fragment |
0001 TCGGCACAGT TTCAACGGAT ATCCTGAATG GGCGCACATC ATTCTTGTCC CTTAGT-CAG
0061 T-CTCACGGG AGTGAAGTGG ATTTGC---C TTCGTTCTAC GAAAGCTTTA ATCCAACTTT
0121 TCTTCCCAAG CTGCCTATCA GAAGATTTCC CCATATGCTT GTGGGGGCAT CAAATGAGCC
0181 TGAGATTTCG -AAAAACCAG CCAGCAATTC CACCATCTAG GCCAATTGAG CCAATATCTG
0241 AGGTGCCGTT TGTGGAACCG GCCTTTGAGA AAAAATACTC TAGGACCCGT GGATTTAGCA
0301 AGGAATTGTC CACACACAGG GATAATGAGA ATGAATATAT CGAGAGCACA GATGAGGTGT
0361 CTCAGATGTT GAGCTTTACT AGCTATACGG ATGAATATGA TAGGGCAGAA CCACTCATTA
0421 CCAAAACGCA AAAGTTTTAT AAAAAGAGAT CCCCCTTGAT TCCCAAATAC GATTCCGAGG
0481 ATTCATATGT GGTCCCTAAG GAAACC |
| Assemble Data |
12866R-3.IR_full 1 TCGGCACAGTTTCAACGGATATCCTGAATGGGCGCACATCATTCTTGTCCCTTAGTCCAG 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
12866R-3.in silico 1 TCGGCACAGTTTCAACGGATATCCTGAATGGGCGCACATCATTCTTGTCCCTTAGT-CAG 60
12866R-3.IR_full 61 TCCTCACGGGAGTGAAGTGGATTTGCCTNCTTCGTTCTACGAAAGCTTTAATCCAACTTT 120
| |||||||||||||||||||||||| |||||||||||||||||||||||||||||||
12866R-3.in silico 61 T-CTCACGGGAGTGAAGTGGATTTGC---CTTCGTTCTACGAAAGCTTTAATCCAACTTT 120
12866R-3.IR_full 121 TCTTCCCAAGCTGCCTATCAGAAGATTTCCCCATATGCTTGTGGGGGCATCAAATGAGCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12866R-3.in silico 121 TCTTCCCAAGCTGCCTATCAGAAGATTTCCCCATATGCTTGTGGGGGCATCAAATGAGCC 180
12866R-3.IR_full 181 TGAGATTTCGNAAAAACCAGCCAGCAATTCCACCATCTAGGCCAATTGAGCCAATATCTG 240
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
12866R-3.in silico 181 TGAGATTTCG-AAAAACCAGCCAGCAATTCCACCATCTAGGCCAATTGAGCCAATATCTG 240
12866R-3.IR_full 241 AGGTGCCGTTTGTGGAACCGGCCTTTGAGAAAAAATACTCTAGGACCCGTGGATTTAGCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12866R-3.in silico 241 AGGTGCCGTTTGTGGAACCGGCCTTTGAGAAAAAATACTCTAGGACCCGTGGATTTAGCA 300
12866R-3.IR_full 301 AGGAATTGTCCACACACAGGGATAATGAGAATGAATATGTCGAGAGCACAGATGAGGTGT 360
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
12866R-3.in silico 301 AGGAATTGTCCACACACAGGGATAATGAGAATGAATATATCGAGAGCACAGATGAGGTGT 360
12866R-3.IR_full 361 CTCAGATGTTGAGCTTTACTAGCTATACGGATGAATATGATAGGGCAGAACCACTCATTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12866R-3.in silico 361 CTCAGATGTTGAGCTTTACTAGCTATACGGATGAATATGATAGGGCAGAACCACTCATTA 420
12866R-3.IR_full 421 CCAAAACGCAAAAGTTTTATAAAAAGAGATCCCCCTTGATTCCCAAATACGATTCCGAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12866R-3.in silico 421 CCAAAACGCAAAAGTTTTATAAAAAGAGATCCCCCTTGATTCCCAAATACGATTCCGAGG 480
12866R-3.IR_full 481 ATTCATATGTGGTCCCTAAGGAAACC 506
||||||||||||||||||||||||||
12866R-3.in silico 481 ATTCATATGTGGTCCCTAAGGAAACC 506
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137136.1 |
CG12866-RA (CG12866), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_142350.1 |
CG11896-RA (CG11896), mRNA |
| 0 |
0 |
1 |
4 |
23 |
NM_169134.1 |
CG31556-RA (CG31556), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080327.2 |
CG3665-RA, transcript variant A (Fas2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165551.2 |
CG30496-RA (CG30496), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135091.2 |
CG31989-RA (Cap-D3), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080121.2 |
CG7719-RA (gwl), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140971.1 |
CG5195-RA (CG5195), mRNA |
| 0 |
0 |
0 |
3 |
25 |
NM_135002.1 |
CG15635-RA (CG15635), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_136864.2 |
CG13185-RA (CG13185), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_130497.2 |
CG13363-RA (Suv4-20), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_139626.1 |
CG1273-RB (CG1273), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168863.1 |
CG32425-RC, transcript variant C (CG32425), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168861.1 |
CG32425-RE, transcript variant E (CG32425), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168860.1 |
CG32425-RA, transcript variant A (CG32425), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_057507.3 |
CG8442-RA (Glu-RI), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_176559.1 |
CG33096-RA, transcript variant A (CG33096), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_176560.1 |
CG33096-RB, transcript variant B (CG33096), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_140745.1 |
CG6052-RA (CG6052), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_078879.2 |
CG10363-RA (TepIV), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_136006.2 |
CG15145-RA (CG15145), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_134676.1 |
CG4213-RA (CG4213), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_133089.2 |
CG6659-RA, transcript variant A (CG6659), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_135567.2 |
CG6187-RA (RluA-2), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_142962.2 |
CG6164-RA (CG6164), mRNA |
| 0 |
0 |
0 |
2 |
13 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_138260.4 |
CG2199-RA, transcript variant A (CG2199), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_167873.1 |
CG2199-RB, transcript variant B (CG2199), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_167574.1 |
CG32556-RA, transcript variant A (CG32556), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_206773.1 |
CG32556-RB, transcript variant B (CG32556), mRNA |
|
|
|
