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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12530R-2 |
| Symbol |
Cdc42 |
Full Name |
Cdc42 |
| CG No |
CG12530 |
Old CG No |
CG12530 |
| Synonyms |
DmCDC42, cdc42, Dcdc42, Dm Cdc42, DCdc42, CDC42, DCDC42, Cdc42Dm, D-CDC42, D-Cdc42, Dmcdc42, CG12530, Cdc42 |
| Accession No (Link to NCBI) |
NM_078690.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Wang H, Qiu Z, Xu Z, Chen SJ, Luo J, Wang X, Chen J.
aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration.
Development (2018) 145(9)
[
PubMed ID = 29636381
]
[
RRC reference
]
Honjo K, Mauthner SE, Wang Y, Skene JHP, Tracey WD Jr.
Nociceptor-Enriched Genes Required for Normal Thermal Nociception.
Cell Rep (2016) 16(2) 295-303
[
PubMed ID = 27346357
]
[
RRC reference
]
Rosa A, Vlassaks E, Pichaud F, Baum B.
Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry.
Dev Cell (2015) 32(5) 604-16
[
PubMed ID = 25703349
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Rotkopf S, Hamberg Y, Aigaki T, Snapper SB, Shilo BZ, Schejter ED.
The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release.
Development (2011) 138(13) 2729-39
[
PubMed ID = 21652648
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCAAACCATCAAGTGCGTG |
| Primer Seq. 3' |
aatctagaggtaccTTTTCAGGCCCTTCTGTGTCA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGTGTAAGA CATGCCTGCT
0061 CATCTCGTAT ACAACCAACA AGTTCCCGTC GGAGTACGTG CCCACGGTGT TCGACAACTA
0121 TGCGGTCACT GTGATGATCG GCGGTGAGCC CTACACACTG GGCCTGTTCG ATACGGCCGG
0181 ACAGGGAGGA TTACGATCGG CTGCGTCCGC TCTCCTATCC GCAGACGGAT GTCTTCCTTG
0241 TCTGCTTTTC GGTGGTCAGT CCCAGTTCCT TCGAGAACGT CAAGGAGAAG TGGGTGCCCG
0301 AGATTACACA CCATTGCCAA AAGACGCCGT TCCTGCTGGT GGGCACACAG ATTGATTTGC
0361 GCGACGAGAA CAGCACGCTG GAGAAGCTGG CCAAGAACAA GCAGAAGCTC CATCACCATG
0421 GAGCAGGGCG AGAAGCTGGC CAAGGAGCTG AAGGCCGTCA AGTACGTGGA GTGCTCGGCC
0481 TTGACACAGA AGGGCCTGAA AA |
| in silico PCR Fragment |
0001 ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGG-GTAAGA CATGCCTGCT
0061 CATCTCGTAT ACAACCAACA AGTTCCCGTC GGAGTACGTG CCCACGGTGT TCGACAACTA
0121 TGCGGTCACT GTGATGATCG GCGGTGAGCC CTACACACTG GGCCTGTTCG ATACGGCCGG
0181 ACA-GGAGGA CTACGATCGG CTGCGTCCGC TCTCCTATCC GCAGACGGAT GTCTTCCTTG
0241 TCTGCTTTTC GGTGGTCAGT CCCAGTTCCT TCGAGAACGT CAAGGAGAAG TGGGTGCCCG
0301 AGATTACACA CCATTGCCAA AAGACGCCGT TCCTGCTGGT GGGCACACAG ATTGATTTGC
0361 GCGACGAGAA CAGCACGCTG GAGAAGCTGG CCAAGAACAA GCAGAAGC-C CATCACCATG
0421 GAGCAGGGCG AGAAGCTGGC CAAGGAGCTG AAGGCCGTCA AGTACGTGGA GTGCTCGGCC
0481 TTGACACAGA AGGGCCTGAA AA |
| Assemble Data |
12530R-2.IR_full 1 ATGCAAACCATCAAGTGCGTGGTCGTCGGCGACGGAGCCGTGGTGTAAGACATGCCTGCT 60
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
12530R-2.in silico 1 ATGCAAACCATCAAGTGCGTGGTCGTCGGCGACGGAGCCGTGG-GTAAGACATGCCTGCT 60
12530R-2.IR_full 61 CATCTCGTATACAACCAACAAGTTCCCGTCGGAGTACGTGCCCACGGTGTTCGACAACTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 61 CATCTCGTATACAACCAACAAGTTCCCGTCGGAGTACGTGCCCACGGTGTTCGACAACTA 120
12530R-2.IR_full 121 TGCGGTCACTGTGATGATCGGCGGTGAGCCCTACACACTGGGCCTGTTCGATACGGCCGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 121 TGCGGTCACTGTGATGATCGGCGGTGAGCCCTACACACTGGGCCTGTTCGATACGGCCGG 180
12530R-2.IR_full 181 ACAGGGAGGATTACGATCGGCTGCGTCCGCTCTCCTATCCGCAGACGGATGTCTTCCTTG 240
||| |||||| |||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 181 ACA-GGAGGACTACGATCGGCTGCGTCCGCTCTCCTATCCGCAGACGGATGTCTTCCTTG 240
12530R-2.IR_full 241 TCTGCTTTTCGGTGGTCAGTCCCAGTTCCTTCGAGAACGTCAAGGAGAAGTGGGTGCCCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 241 TCTGCTTTTCGGTGGTCAGTCCCAGTTCCTTCGAGAACGTCAAGGAGAAGTGGGTGCCCG 300
12530R-2.IR_full 301 AGATTACACACCATTGCCAAAAGACGCCGTTCCTGCTGGTGGGCACACAGATTGATTTGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 301 AGATTACACACCATTGCCAAAAGACGCCGTTCCTGCTGGTGGGCACACAGATTGATTTGC 360
12530R-2.IR_full 361 GCGACGAGAACAGCACGCTGGAGAAGCTGGCCAAGAACAAGCAGAAGCTCCATCACCATG 420
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
12530R-2.in silico 361 GCGACGAGAACAGCACGCTGGAGAAGCTGGCCAAGAACAAGCAGAAGC-CCATCACCATG 420
12530R-2.IR_full 421 GAGCAGGGCGAGAAGCTGGCCAAGGAGCTGAAGGCCGTCAAGTACGTGGAGTGCTCGGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12530R-2.in silico 421 GAGCAGGGCGAGAAGCTGGCCAAGGAGCTGAAGGCCGTCAAGTACGTGGAGTGCTCGGCC 480
12530R-2.IR_full 481 TTGACACAGAAGGGCCTGAAAA 502
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12530R-2.in silico 481 TTGACACAGAAGGGCCTGAAAA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
8 |
NM_078690.2 |
CG12530-RA, transcript variant A (Cdc42), mRNA |
| 100 |
481 |
0 |
0 |
8 |
NM_167677.1 |
CG12530-RB, transcript variant B (Cdc42), mRNA |
| 1.03 |
5 |
25 |
41 |
61 |
NM_139864.1 |
CG8556-RA (Rac2), mRNA |
| 0.2 |
1 |
2 |
5 |
10 |
NM_166986.2 |
CG2849-RA, transcript variant A (Rala), mRNA |
| 0.2 |
1 |
2 |
5 |
10 |
NM_080324.3 |
CG2849-RC, transcript variant C (Rala), mRNA |
| 0.2 |
1 |
2 |
5 |
10 |
NM_166985.2 |
CG2849-RB, transcript variant B (Rala), mRNA |
| 0 |
0 |
21 |
68 |
65 |
NM_057602.3 |
CG2248-RA (Rac1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_166139.2 |
CG8416-RD, transcript variant D (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_206127.1 |
CG8416-RG, transcript variant G (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_206129.1 |
CG8416-RE, transcript variant E (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_134309.1 |
CG8416-RC, transcript variant C (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_057750.3 |
CG8416-RA, transcript variant A (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_206128.1 |
CG8416-RF, transcript variant F (Rho1), mRNA |
| 0 |
0 |
8 |
2 |
12 |
NM_134308.2 |
CG8416-RB, transcript variant B (Rho1), mRNA |
| 0 |
0 |
7 |
11 |
12 |
NM_001042801.1 |
CG34104-RB, transcript variant B (CG34104), mRNA |
| 0 |
0 |
7 |
4 |
2 |
NM_001042802.1 |
CG34104-RA, transcript variant A (CG34104), mRNA |
| 0 |
0 |
6 |
7 |
8 |
NM_167956.1 |
CG1044-RB, transcript variant B (dos), mRNA |
| 0 |
0 |
6 |
7 |
8 |
NM_079166.2 |
CG1044-RA, transcript variant A (dos), mRNA |
| 0 |
0 |
5 |
13 |
9 |
NM_133136.1 |
CG7874-RA (CG7874), mRNA |
| 0 |
0 |
3 |
32 |
33 |
NM_170343.1 |
CG5588-RA, transcript variant A (Mtl), mRNA |
| 0 |
0 |
3 |
32 |
33 |
NM_079809.2 |
CG5588-RB, transcript variant B (Mtl), mRNA |
| 0 |
0 |
3 |
32 |
33 |
NM_170344.1 |
CG5588-RC, transcript variant C (Mtl), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_169419.1 |
CG18476-RA (CG18476), mRNA |
| 0 |
0 |
3 |
3 |
7 |
NM_078523.2 |
CG2252-RB, transcript variant B (fs(1)h), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_167144.2 |
CG2252-RA, transcript variant A (fs(1)h), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_206647.1 |
CG2252-RC, transcript variant C (fs(1)h), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_206646.1 |
CG2252-RD, transcript variant D (fs(1)h), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_206645.1 |
CG2252-RE, transcript variant E (fs(1)h), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_132195.2 |
CG1515-RA (l(1)G0155), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_135797.2 |
CG6523-RA (CG6523), mRNA |
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