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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12489R-2 |
| Symbol |
Dnr1 |
Full Name |
Defense repressor 1 |
| CG No |
CG12489 |
Old CG No |
CG12489 |
| Synonyms |
CG12489, Dnr1 |
| Accession No (Link to NCBI) |
NM_137836.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Guntermann S, Primrose DA, Foley E.
Dnr1-dependent regulation of the Drosophila immune deficiency signaling pathway.
Dev Comp Immunol (2008) 33(1) 127-34
[
PubMed ID = 18775745
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGCGGACTATCTGCGCCAAAT |
| Primer Seq. 3' |
aatctagaggtaccTGTCGAAGACATAGCGCTTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG CTGGCCAAGC TGCAGATGAG
0061 CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG GATCTCGAAG GCTACGGCGA
0121 GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG CGCTGCGACA TTGCGGTGGG
0181 CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG AGCATCCCCT TTGGCGCCAT
0241 CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG TACGTGGATG ACCACAACGA
0301 TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC GCCGCGGGCC TGTACCGCTC
0361 CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG CGCGGAGTGG TGACCAATCA
0421 GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC ATGGAGAATA CGGAGCTgGG
0481 CAAGCGCTAT GTCTTCGACA |
| in silico PCR Fragment |
0001 GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG CTGGCCAAGC TGCAGATGAG
0061 CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG GATCTCGAAG GCTACGGCGA
0121 GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG CGCTGCGACA TTGCGGTGGG
0181 CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG AGCATCCCCT TTGGCGCCAT
0241 CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG TACGTGGATG ACCACAACGA
0301 TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC GCCGCGGGCC TGTACCGCTC
0361 CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG CGCGGAGTGG TGACCAATCA
0421 GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC ATGGAGAATA CGGAGCTGGG
0481 CAAGCGCTAT GTCTTCGACA |
| Assemble Data |
12489R-2.IR_full 1 GGCGGACTATCTGCGCCAAATAGCCGTGGAGCACGGCAAGCTGGCCAAGCTGCAGATGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 1 GGCGGACTATCTGCGCCAAATAGCCGTGGAGCACGGCAAGCTGGCCAAGCTGCAGATGAG 60
12489R-2.IR_full 61 CCTCAAGACGGCCAAGTACTGGCTGCTCAAGTCCATCCAGGATCTCGAAGGCTACGGCGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 61 CCTCAAGACGGCCAAGTACTGGCTGCTCAAGTCCATCCAGGATCTCGAAGGCTACGGCGA 120
12489R-2.IR_full 121 GGAGCTCTTTAGCGGCGTGACCACCAACGAGAGTGCCACGCGCTGCGACATTGCGGTGGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 121 GGAGCTCTTTAGCGGCGTGACCACCAACGAGAGTGCCACGCGCTGCGACATTGCGGTGGG 180
12489R-2.IR_full 181 CGCCCATGGCATCACCGTCTGCCGGGGGGGCGAGAAACAGAGCATCCCCTTTGGCGCCAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 181 CGCCCATGGCATCACCGTCTGCCGGGGGGGCGAGAAACAGAGCATCCCCTTTGGCGCCAT 240
12489R-2.IR_full 241 CGCTGCGGCCAAGTCGCTGCGTCGCACTTTCAAGTTGGAGTACGTGGATGACCACAACGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 241 CGCTGCGGCCAAGTCGCTGCGTCGCACTTTCAAGTTGGAGTACGTGGATGACCACAACGA 300
12489R-2.IR_full 301 TCGCAAGGAGCTGGAGATCAAGCTGCCCAAGCAGCCGATCGCCGCGGGCCTGTACCGCTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 301 TCGCAAGGAGCTGGAGATCAAGCTGCCCAAGCAGCCGATCGCCGCGGGCCTGTACCGCTC 360
12489R-2.IR_full 361 CATCACGGAGCGCCACGCCTTCTACGTGTGCGACAAGGTGCGCGGAGTGGTGACCAATCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 361 CATCACGGAGCGCCACGCCTTCTACGTGTGCGACAAGGTGCGCGGAGTGGTGACCAATCA 420
12489R-2.IR_full 421 GTTTACCCGGGATCTCAAGGGCACCATCGCCTCCATGTTCATGGAGAATACGGAGCTGGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-2.in silico 421 GTTTACCCGGGATCTCAAGGGCACCATCGCCTCCATGTTCATGGAGAATACGGAGCTGGG 480
12489R-2.IR_full 481 CAAGCGCTATGTCTTCGACA 500
||||||||||||||||||||
12489R-2.in silico 481 CAAGCGCTATGTCTTCGACA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_137836.1 |
CG12489-RA (Dnr1), mRNA |
| 0 |
0 |
3 |
6 |
10 |
NM_132018.2 |
CG3149-RA (CG3149), mRNA |
| 0 |
0 |
3 |
3 |
6 |
NM_143006.1 |
CG13617-RA (CG13617), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_165970.1 |
CG4062-RB, transcript variant B (Aats-val), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_080099.2 |
CG4062-RA, transcript variant A (Aats-val), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_140625.2 |
CG4818-RA (CG4818), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_078656.3 |
CG9108-RA (RSG7), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132132.1 |
CG4557-RA (CG4557), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_078563.2 |
CG18361-RA, transcript variant A (dsh), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167279.1 |
CG18361-RB, transcript variant B (dsh), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176113.1 |
CG2049-RD, transcript variant D (Pkn), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176112.1 |
CG2049-RF, transcript variant F (Pkn), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132530.2 |
CG1847-RA, transcript variant A (CG1847), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167311.1 |
CG1847-RB, transcript variant B (CG1847), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140466.1 |
CG5295-RA (CG5295), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176111.1 |
CG2049-RC, transcript variant C (Pkn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176110.1 |
CG2049-RB, transcript variant B (Pkn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132065.1 |
CG4660-RA (CG4660), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_079088.2 |
CG9952-RA (ppa), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_143618.2 |
CG1774-RA (CG1774), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167521.2 |
CG32575-RA, transcript variant A (hang), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167520.2 |
CG32575-RB, transcript variant B (hang), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137110.2 |
CG8617-RA (CG8617), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001031967.1 |
CG7749-RB, transcript variant B (fat2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140914.2 |
CG7749-RA, transcript variant A (fat2), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_078689.2 |
CG14228-RA (Mer), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_167164.2 |
CG12737-RB, transcript variant B (Crag), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_080341.2 |
CG12737-RA, transcript variant A (Crag), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_078582.3 |
CG32659-RA (Ten-a), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001043134.1 |
CG7368-RB (CG7368), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
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