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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12489R-1

Symbol

Dnr1

Full Name

Defense repressor 1

CG No

CG12489

Old CG No

CG12489

Synonyms

CG12489, Dnr1

Accession No (Link to NCBI)

NM_137836.1

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Guntermann S, Primrose DA, Foley E.
Dnr1-dependent regulation of the Drosophila immune deficiency signaling pathway.
Dev Comp Immunol (2008) 33(1) 127-34 [ PubMed ID = 18775745 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCGGACTATCTGCGCCAAAT

Primer Seq. 3'

aatctagaggtaccTGTCGAAGACATAGCGCTTGC

Predicted Fragment Size

IR fragment full Seq

0001 GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG CTGGCCAAGC TGCAGATGAG 
0061 CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG GATCTCGAAG GCTACGGCGA 
0121 GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG CGCTGCGACA TTGCGGTGGG 
0181 CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG AGCATCCCCT TTGGCGCCAT 
0241 CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG TACGTGGATG ACCACAACGA 
0301 TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC GCCGCGGGCC TGTACCGCTC 
0361 CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG CGCGGAGTGG TGACCAATCA 
0421 GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC ATGGAGAATA CGGAGCTgGG 
0481 CAAGCGCTAT GTCTTCGACA

in silico PCR Fragment

0001 GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG CTGGCCAAGC TGCAGATGAG 
0061 CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG GATCTCGAAG GCTACGGCGA 
0121 GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG CGCTGCGACA TTGCGGTGGG 
0181 CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG AGCATCCCCT TTGGCGCCAT 
0241 CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG TACGTGGATG ACCACAACGA 
0301 TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC GCCGCGGGCC TGTACCGCTC 
0361 CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG CGCGGAGTGG TGACCAATCA 
0421 GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC ATGGAGAATA CGGAGCTGGG 
0481 CAAGCGCTAT GTCTTCGACA

Assemble Data


12489R-1.IR_full       1   GGCGGACTATCTGCGCCAAATAGCCGTGGAGCACGGCAAGCTGGCCAAGCTGCAGATGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     1   GGCGGACTATCTGCGCCAAATAGCCGTGGAGCACGGCAAGCTGGCCAAGCTGCAGATGAG 60


12489R-1.IR_full       61  CCTCAAGACGGCCAAGTACTGGCTGCTCAAGTCCATCCAGGATCTCGAAGGCTACGGCGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     61  CCTCAAGACGGCCAAGTACTGGCTGCTCAAGTCCATCCAGGATCTCGAAGGCTACGGCGA 120


12489R-1.IR_full       121 GGAGCTCTTTAGCGGCGTGACCACCAACGAGAGTGCCACGCGCTGCGACATTGCGGTGGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     121 GGAGCTCTTTAGCGGCGTGACCACCAACGAGAGTGCCACGCGCTGCGACATTGCGGTGGG 180


12489R-1.IR_full       181 CGCCCATGGCATCACCGTCTGCCGGGGGGGCGAGAAACAGAGCATCCCCTTTGGCGCCAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     181 CGCCCATGGCATCACCGTCTGCCGGGGGGGCGAGAAACAGAGCATCCCCTTTGGCGCCAT 240


12489R-1.IR_full       241 CGCTGCGGCCAAGTCGCTGCGTCGCACTTTCAAGTTGGAGTACGTGGATGACCACAACGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     241 CGCTGCGGCCAAGTCGCTGCGTCGCACTTTCAAGTTGGAGTACGTGGATGACCACAACGA 300


12489R-1.IR_full       301 TCGCAAGGAGCTGGAGATCAAGCTGCCCAAGCAGCCGATCGCCGCGGGCCTGTACCGCTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     301 TCGCAAGGAGCTGGAGATCAAGCTGCCCAAGCAGCCGATCGCCGCGGGCCTGTACCGCTC 360


12489R-1.IR_full       361 CATCACGGAGCGCCACGCCTTCTACGTGTGCGACAAGGTGCGCGGAGTGGTGACCAATCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     361 CATCACGGAGCGCCACGCCTTCTACGTGTGCGACAAGGTGCGCGGAGTGGTGACCAATCA 420


12489R-1.IR_full       421 GTTTACCCGGGATCTCAAGGGCACCATCGCCTCCATGTTCATGGAGAATACGGAGCTGGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12489R-1.in silico     421 GTTTACCCGGGATCTCAAGGGCACCATCGCCTCCATGTTCATGGAGAATACGGAGCTGGG 480


12489R-1.IR_full       481 CAAGCGCTATGTCTTCGACA 500
                           ||||||||||||||||||||
12489R-1.in silico     481 CAAGCGCTATGTCTTCGACA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	6	NM_137836.1	CG12489-RA (Dnr1), mRNA
0	0	3	6	10	NM_132018.2	CG3149-RA (CG3149), mRNA
0	0	3	3	6	NM_143006.1	CG13617-RA (CG13617), mRNA
0	0	2	5	7	NM_165970.1	CG4062-RB, transcript variant B (Aats-val), mRNA
0	0	2	5	7	NM_080099.2	CG4062-RA, transcript variant A (Aats-val), mRNA
0	0	2	5	2	NM_140625.2	CG4818-RA (CG4818), mRNA
0	0	2	4	6	NM_078656.3	CG9108-RA (RSG7), mRNA
0	0	2	3	5	NM_132132.1	CG4557-RA (CG4557), mRNA
0	0	2	3	3	NM_078563.2	CG18361-RA, transcript variant A (dsh), mRNA
0	0	2	3	3	NM_167279.1	CG18361-RB, transcript variant B (dsh), mRNA
0	0	2	2	3	NM_176113.1	CG2049-RD, transcript variant D (Pkn), mRNA
0	0	2	2	3	NM_176112.1	CG2049-RF, transcript variant F (Pkn), mRNA
0	0	2	2	3	NM_132530.2	CG1847-RA, transcript variant A (CG1847), mRNA
0	0	2	2	3	NM_167311.1	CG1847-RB, transcript variant B (CG1847), mRNA
0	0	2	2	2	NM_140466.1	CG5295-RA (CG5295), mRNA
0	0	2	2	2	NM_176111.1	CG2049-RC, transcript variant C (Pkn), mRNA
0	0	2	2	2	NM_176110.1	CG2049-RB, transcript variant B (Pkn), mRNA
0	0	2	2	2	NM_132065.1	CG4660-RA (CG4660), mRNA
0	0	1	5	6	NM_079088.2	CG9952-RA (ppa), mRNA
0	0	1	4	7	NM_143618.2	CG1774-RA (CG1774), mRNA
0	0	1	4	6	NM_167521.2	CG32575-RA, transcript variant A (hang), mRNA
0	0	1	4	6	NM_167520.2	CG32575-RB, transcript variant B (hang), mRNA
0	0	1	3	5	NM_137110.2	CG8617-RA (CG8617), mRNA
0	0	1	3	2	NM_001031967.1	CG7749-RB, transcript variant B (fat2), mRNA
0	0	1	3	2	NM_140914.2	CG7749-RA, transcript variant A (fat2), mRNA
0	0	1	2	10	NM_078689.2	CG14228-RA (Mer), mRNA
0	0	1	2	7	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0	0	1	2	7	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	1	2	7	NM_078582.3	CG32659-RA (Ten-a), mRNA
0	0	1	2	6	NM_001043134.1	CG7368-RB (CG7368), mRNA

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