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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12399R-1 |
| Symbol |
Mad |
Full Name |
Mothers against dpp |
| CG No |
CG12399 |
Old CG No |
CG12399 |
| Synonyms |
mad, pMad, p-Mad, pMAD, MAD, 2/23, l(2)K00237, apg, l(2)k00237, En(vvl), E(zen)2, CG12399, Mad, P-mad |
| Accession No (Link to NCBI) |
NM_057669.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Lu Y, Yao Y, Li Z.
Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis.
Sci Rep (2019) 9(1) 6118
[
PubMed ID = 30992503
]
[
RRC reference
]
Eusebio N, Tavares L, Pereira PS.
CtBP represses Dpp-dependent Mad activation during Drosophila eye development.
Dev Biol (2018) 442(1) 188-198
[
PubMed ID = 30031756
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Xu R, Li J, Zhao H, Kong R, Wei M, Shi L, Bai G, Li Z.
Self-restrained regulation of stem cell niche activity by niche components in the Drosophila testis.
Dev Biol (2018) 439(1) 42-51
[
PubMed ID = 29679558
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Sato T, Ogata J, Niki Y.
BMP and Hh signaling affects primordial germ cell division in Drosophila.
Zoolog Sci (2010) 27(10) 804-10
[
PubMed ID = 20887178
]
[
RRC reference
]
Boulanger A, Farge M, Ramanoudjame C, Wharton K, Dura JM.
Drosophila motor neuron retraction during metamorphosis is mediated by inputs from TGF-β/BMP signaling and orphan nuclear receptors.
PLoS One (2012) 7(7) e40255
[
PubMed ID = 22792255
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATGTGGAATCGAACACAAGC |
| Primer Seq. 3' |
aatctagaggtaccTGGTTGAACTGCAGCATCGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GATGTGGAAT CGAACACAAG CAGCGCGATG TCCACACTGG GCTCGCTATT CTCCTTCACA
0061 TCGCCGGCGG TGAAGAAGCT GCTGGGCTGG AAACAGGGTG ACGAAGAGGA GAAGTGGGCG
0121 GAGAAGGCCG TCGACAGTCT GGTGAAGAAG TTGAANGAAG CGCAAGGGCG CCATCGAGGA
0181 TGCATGGAGC GTGCGCTCTC CTGTCCCGGT CAGCCCTCGA AGTGTGTCAC CATTCCATGC
0241 TCGCTGGACG GACGATTACA GGTCTCCCAT CGCAAGGGTC TGCCGCATGT GATCTAGCTG
0301 CCGCGTGTGG NGCTGGCCAG ATGCTGCAGT CGCACCACGA ACTGAAGCCA CTCGAGCTGT
0361 GCCAGTATCC GTTGCAGCGC CAAGCAGAAG GAGGNGTGCA TCAATCCGTA TCACTATAAG
0421 CGCGTGGAGA GTCCGgtGNT CCCGCCAGTA CTCGTTCCTC GCCACTCGGA ATTCGCGCCC
0481 GGTCACTCGA TGCTGCAGTT CAACCA |
| in silico PCR Fragment |
0001 GATGTGGAAT CGAACACAAG CAGCGCGATG TCCACACTGG GCTCGCTATT CTCCTTCACA
0061 TCGCCGGCGG TGAAGAAGCT GCTGGGCTGG AAACAGGGTG ACGAAGAGGA GAAGTGGGCG
0121 GAGAAGGCCG TCGACAGTCT GGTGAAGAAG TTGAA-GAAG CGCAAGGGCG CCATCGAGGA
0181 -GC-TGGAGC GTGCGCTCTC CTGTCCCGGT CAGCCCTCGA AGTGTGTCAC CATTCCACGC
0241 TCGCTGGACG GACGATTACA GGTCTCCCAT CGCAAGGGTC TGCCGCATGT GATCTA-CTG
0301 CCGCGTGTGG CGCTGGCCAG AT-CTGCAGT CGCACCACGA ACTGAAGCCA CTCGAGCTGT
0361 GCCAGTATCC GTT-CAGCGC CAAGCAGAAG GAGGTGTGCA TCAATCCGTA TCACTATAAG
0421 CGCGTGGAGA GTCCGGTGCT CCCGCCAGTA CTCGTTCCTC GCCACTCGGA ATTCGCGCCC
0481 GGTCACTCGA TGCTGCAGTT CAACCA |
| Assemble Data |
12399R-1.IR_full 1 GATGTGGAATCGAACACAAGCAGCGCGATGTCCACACTGGGCTCGCTATTCTCCTTCACA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12399R-1.in silico 1 GATGTGGAATCGAACACAAGCAGCGCGATGTCCACACTGGGCTCGCTATTCTCCTTCACA 60
12399R-1.IR_full 61 TCGCCGGCGGTGAAGAAGCTGCTGGGCTGGAAACAGGGTGACGAAGAGGAGAAGTGGGCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12399R-1.in silico 61 TCGCCGGCGGTGAAGAAGCTGCTGGGCTGGAAACAGGGTGACGAAGAGGAGAAGTGGGCG 120
12399R-1.IR_full 121 GAGAAGGCCGTCGACAGTCTGGTGAAGAAGTTGAANGAAGCGCAAGGGCGCCATCGAGGA 180
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
12399R-1.in silico 121 GAGAAGGCCGTCGACAGTCTGGTGAAGAAGTTGAA-GAAGCGCAAGGGCGCCATCGAGGA 180
12399R-1.IR_full 181 TGCATGGAGCGTGCGCTCTCCTGTCCCGGTCAGCCCTCGAAGTGTGTCACCATTCCATGC 240
|| ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
12399R-1.in silico 181 -GC-TGGAGCGTGCGCTCTCCTGTCCCGGTCAGCCCTCGAAGTGTGTCACCATTCCACGC 240
12399R-1.IR_full 241 TCGCTGGACGGACGATTACAGGTCTCCCATCGCAAGGGTCTGCCGCATGTGATCTAGCTG 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
12399R-1.in silico 241 TCGCTGGACGGACGATTACAGGTCTCCCATCGCAAGGGTCTGCCGCATGTGATCTA-CTG 300
12399R-1.IR_full 301 CCGCGTGTGGNGCTGGCCAGATGCTGCAGTCGCACCACGAACTGAAGCCACTCGAGCTGT 360
|||||||||| ||||||||||| |||||||||||||||||||||||||||||||||||||
12399R-1.in silico 301 CCGCGTGTGGCGCTGGCCAGAT-CTGCAGTCGCACCACGAACTGAAGCCACTCGAGCTGT 360
12399R-1.IR_full 361 GCCAGTATCCGTTGCAGCGCCAAGCAGAAGGAGGNGTGCATCAATCCGTATCACTATAAG 420
||||||||||||| |||||||||||||||||||| |||||||||||||||||||||||||
12399R-1.in silico 361 GCCAGTATCCGTT-CAGCGCCAAGCAGAAGGAGGTGTGCATCAATCCGTATCACTATAAG 420
12399R-1.IR_full 421 CGCGTGGAGAGTCCGGTGNTCCCGCCAGTACTCGTTCCTCGCCACTCGGAATTCGCGCCC 480
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
12399R-1.in silico 421 CGCGTGGAGAGTCCGGTGCTCCCGCCAGTACTCGTTCCTCGCCACTCGGAATTCGCGCCC 480
12399R-1.IR_full 481 GGTCACTCGATGCTGCAGTTCAACCA 506
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12399R-1.in silico 481 GGTCACTCGATGCTGCAGTTCAACCA 506
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057669.2 |
CG12399-RA (Mad), mRNA |
| 0 |
0 |
5 |
29 |
43 |
NM_078524.2 |
CG2262-RA (Smox), mRNA |
| 0 |
0 |
4 |
2 |
6 |
NM_132260.2 |
CG12113-RA (l(1)G0095), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_057659.2 |
CG9652-RA (DopR), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_136046.2 |
CG15161-RA (CG15161), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_143770.2 |
CG3845-RB, transcript variant B (l(2)01424), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_001038854.1 |
CG3845-RC, transcript variant C (l(2)01424), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_165961.1 |
CG3845-RA, transcript variant A (l(2)01424), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_079987.2 |
CG1263-RA, transcript variant A (RpL8), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_167955.1 |
CG1263-RB, transcript variant B (RpL8), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057685.3 |
CG11527-RA (Tig), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206424.1 |
CG7448-RB, transcript variant B (CG7448), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_141116.1 |
CG7448-RA, transcript variant A (CG7448), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057379.2 |
CG8355-RC, transcript variant C (sli), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057381.2 |
CG8355-RB, transcript variant B (sli), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057380.2 |
CG8355-RA, transcript variant A (sli), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_138049.2 |
CG3328-RA (CG3328), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_143402.2 |
CG1420-RA (CG1420), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_166704.1 |
CG15792-RB, transcript variant B (zip), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001014553.1 |
CG15792-RC, transcript variant C (zip), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079136.2 |
CG15792-RA, transcript variant A (zip), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001014552.1 |
CG15792-RD, transcript variant D (zip), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137148.2 |
CG10202-RA (CG10202), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134559.1 |
CG1829-RA (Cyp6v1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142557.1 |
CG7333-RA (CG7333), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079349.2 |
CG5185-RA (Tom), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143995.2 |
CG13631-RA (CG13631), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_136533.1 |
CG11641-RA (pdm3), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_136942.2 |
CG8545-RA (CG8545), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_140935.2 |
CG17145-RA (CG17145), mRNA |
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