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Notification of resumption of orders:
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Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.
Thank you for your understanding.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12370R-4 |
| Symbol |
CG12370 |
Full Name |
CG12370 |
| CG No |
CG12370 |
Old CG No |
CG12370 |
| Synonyms |
unnamed, CG13156, anon-WO0170980.104, anon-WO0170980.103, CG12370 |
| Accession No (Link to NCBI) |
NM_165907.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
King AN, Barber AF, Smith AE, Dreyer AP, Sitaraman D, Nitabach MN, Cavanaugh DJ, Sehgal A.
A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity.
Curr. Biol. (2017) 27(13) 1915-1927.e5
[
PubMed ID = 28669757
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTCAAGGGCGTGCACTACGA |
| Primer Seq. 3' |
aatctagaggtaccAGCCGATGAGGGCGTAGATAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATTCAAGGGC GTGCACTACG ACACCACAGA CAATGCCACC CGCTTTTGCT TTCCAAACGG
0061 AACGTGGGAT CACTATTCGG ACTATGACCG CTGTCACCAG AACTCGGGCT CCATACCGGT
0121 GGTGCCCGAC TTCTCACCCA ACGTCGAACT GCCGGCCATC ATATACTGCC GGCGGTTATT
0181 TCCTGACGCT TCGCCACCTT GGTGGTVGCT CTCATCATAT TCCTCAGCTT TAAAGATCTT
0241 CGTTGCCTGC GAAACACCAT TCATGCCAAT CTGTTCCTCA CCTACGATCA CATCCGCACT
0301 CCTCTGGATA CTCACACTGT TCCTGCAAGT GATAACCACA GAGTCTAGTC AGGCTGGCTG
0361 CATAACGTTG GTAATCATGT TTCAGTACTT TTACCTAACC AACTTTTTCT GGATGTTTGT
0421 GGAGGGCCTC TATCTGTACA CGCTGGTGGT GCAAACATTC TCCAGTGATA ACATTAGCTT
0481 TATTATCTAC GCCCTCATCG GCT |
| in silico PCR Fragment |
0001 ATTCAAGGGC GTGCACTACG ACACCACAGA CAATGCCACC CGCTTTTGCT TTCCAAACGG
0061 AACGTGGGAT CACTATTCGG ACTATGACCG CTGTCACCAG AACTCGGGCT CCATACCGGT
0121 GGTGCCCGAC TTCTCACCCA ACGTCGAACT GCCGGCCATC ATATA-TGCC GGCGGTTATT
0181 TCCTGA-GCT TCGCCACCTT GGTGGTGGCT CTCATCATAT TCCTCAGCTT TAAAGATCTT
0241 CGTTGCCTGC GAAACACCAT TCATGCCAAT TTGTTCCTCA CCTAC-ATCA CATCCGCACT
0301 CCTCTGGATA CTCACACTGT TCCTGCAAGT GATAACCACA GAGTCTAGTC AGGCTGGCTG
0361 CATAACGTTG GTAATCATGT TTCAGTACTT TTACCTAACC AACTTTTTCT GGATGTTTGT
0421 GGAGGGCCTC TATCTGTACA CGCTGGTGGT GCAAACATTC TCCAGTGATA ACATTAGCTT
0481 TATTATCTAC GCCCTCATCG GCT |
| Assemble Data |
12370R-4.IR_full 1 ATTCAAGGGCGTGCACTACGACACCACAGACAATGCCACCCGCTTTTGCTTTCCAAACGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12370R-4.in silico 1 ATTCAAGGGCGTGCACTACGACACCACAGACAATGCCACCCGCTTTTGCTTTCCAAACGG 60
12370R-4.IR_full 61 AACGTGGGATCACTATTCGGACTATGACCGCTGTCACCAGAACTCGGGCTCCATACCGGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12370R-4.in silico 61 AACGTGGGATCACTATTCGGACTATGACCGCTGTCACCAGAACTCGGGCTCCATACCGGT 120
12370R-4.IR_full 121 GGTGCCCGACTTCTCACCCAACGTCGAACTGCCGGCCATCATATACTGCCGGCGGTTATT 180
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
12370R-4.in silico 121 GGTGCCCGACTTCTCACCCAACGTCGAACTGCCGGCCATCATATA-TGCCGGCGGTTATT 180
12370R-4.IR_full 181 TCCTGACGCTTCGCCACCTTGGTGGTVGCTCTCATCATATTCCTCAGCTTTAAAGATCTT 240
|||||| ||||||||||||||||||| |||||||||||||||||||||||||||||||||
12370R-4.in silico 181 TCCTGA-GCTTCGCCACCTTGGTGGTGGCTCTCATCATATTCCTCAGCTTTAAAGATCTT 240
12370R-4.IR_full 241 CGTTGCCTGCGAAACACCATTCATGCCAATCTGTTCCTCACCTACGATCACATCCGCACT 300
|||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||||
12370R-4.in silico 241 CGTTGCCTGCGAAACACCATTCATGCCAATTTGTTCCTCACCTAC-ATCACATCCGCACT 300
12370R-4.IR_full 301 CCTCTGGATACTCACACTGTTCCTGCAAGTGATAACCACAGAGTCTAGTCAGGCTGGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12370R-4.in silico 301 CCTCTGGATACTCACACTGTTCCTGCAAGTGATAACCACAGAGTCTAGTCAGGCTGGCTG 360
12370R-4.IR_full 361 CATAACGTTGGTAATCATGTTTCAGTACTTTTACCTAACCAACTTTTTCTGGATGTTTGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12370R-4.in silico 361 CATAACGTTGGTAATCATGTTTCAGTACTTTTACCTAACCAACTTTTTCTGGATGTTTGT 420
12370R-4.IR_full 421 GGAGGGCCTCTATCTGTACACGCTGGTGGTGCAAACATTCTCCAGTGATAACATTAGCTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12370R-4.in silico 421 GGAGGGCCTCTATCTGTACACGCTGGTGGTGCAAACATTCTCCAGTGATAACATTAGCTT 480
12370R-4.IR_full 481 TATTATCTACGCCCTCATCGGCT 503
|||||||||||||||||||||||
12370R-4.in silico 481 TATTATCTACGCCCTCATCGGCT 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165907.3 |
CG12370-RA (CG12370), mRNA |
| 0 |
0 |
2 |
9 |
46 |
NM_137116.2 |
CG8422-RA (CG8422), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_139478.1 |
CG1143-RA (CG1143), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_169429.3 |
CG14723-RA, transcript variant A (HisCl1), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_141859.3 |
CG14723-RB, transcript variant B (HisCl1), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_206474.1 |
CG14723-RC, transcript variant C (HisCl1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134659.2 |
CG11490-RA (CG11490), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080118.2 |
CG2075-RA (aly), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142326.2 |
CG16941-RA (CG16941), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138131.2 |
CG9047-RA, transcript variant A (CG9047), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166693.1 |
CG9047-RB, transcript variant B (CG9047), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166694.1 |
CG9047-RC, transcript variant C (CG9047), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165179.1 |
CG31812-RA (CG31812), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_132615.1 |
CG4395-RA (CG4395), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166560.1 |
CG30271-RC (CG30271), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136664.1 |
CG1863-RA, transcript variant A (CG1863), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_206064.1 |
CG1863-RB, transcript variant B (CG1863), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136208.2 |
CG9317-RA, transcript variant A (CG9317), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165340.1 |
CG9317-RB, transcript variant B (CG9317), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132262.2 |
CG11265-RA, transcript variant A (CG11265), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206660.1 |
CG11265-RB, transcript variant B (CG11265), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206659.1 |
CG11265-RC, transcript variant C (CG11265), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080254.2 |
CG6392-RA (cmet), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168542.1 |
CG10171-RB, transcript variant B (CG10171), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140382.1 |
CG10171-RA, transcript variant A (CG10171), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169673.1 |
CG4699-RC, transcript variant C (CG4699), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165270.1 |
CG31792-RA (CG31792), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169672.1 |
CG4699-RB, transcript variant B (CG4699), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142235.1 |
CG4699-RA, transcript variant A (CG4699), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_134748.2 |
CG5423-RA (robo3), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| + |
|
| VB |
 |
| |
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