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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12297R-1 |
| Symbol |
BG4 |
Full Name |
BG4 |
| CG No |
CG12297 |
Old CG No |
CG12297 |
| Synonyms |
dFADD, FADD, Fadd, dFadd, CG12297, BG4 |
| Accession No (Link to NCBI) |
NM_142749.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Neyen C, Bretscher AJ, Binggeli O, Lemaitre B.
Methods to study Drosophila immunity.
Methods (2014) 68(1) 116-28
[
PubMed ID = 24631888
]
[
RRC reference
]
Buchon N, Broderick NA, Chakrabarti S, Lemaitre B.
Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila.
Genes Dev (2009) 23(19) 2333-44
[
PubMed ID = 19797770
]
[
RRC reference
]
Nelson B, Freisinger T, Ishii K, Okado K, Shinzawa N, Fukumoto S, Kanuka H.
Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila.
Biochem Biophys Res Commun (2013) 430(3) 1120-5
[
PubMed ID = 23261474
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.
Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.
Cell Death Differ (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
Leulier F, Vidal S, Saigo K, Ueda R, Lemaitre B.
Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults.
Curr Biol (2002) 12(12) 996-1000
[
PubMed ID = 12123572
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGACTGCAGGCAGGCACTGGA |
| Primer Seq. 3' |
aatctagaggtaccTCGCCAATAGCGACCCAGTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGACTGCAgG CAGGCACTGG AGCTACGACA AGCCTCAAAC AAATCGCCAT TGATGGATGC
0061 ACCGAGAACG TGGAGCAGTT GAAACTAATT TTTGTCGAAG AGATTGGTTC GCGACGCAGA
0121 TCGGATTGCA TACGAACAAT CGAGGATTTA ATTGACTGCC TGGAACGAGC GGACGAACTG
0181 TCGGAGTACA ATGTTGAGCC ACTGCGCCNG GATGTCAGGC AATATGCCAC AGCTCATCGA
0241 GGCTCTGAGC GCCTACACAC CGCCGGAAAA CATTCTTGGA CATCCGGTTA ATCTGTACCA
0301 GGAACTACGA TTGGCCGAGG AACTTCGTCA GCAACTCCGG ATTGCTCCAG CTTCCCAGAA
0361 TGCTCAGCCA TCAGTTTCCG AAtNNGNTGC CGCTGTGCCT CCAACAGCAA TCCAAAACTA
0421 TGCCACCCCA GCTGCTTTTA CGGATCACAA ACGAACGATG GTCTTTAAGA AAATATCCGA
0481 GGAACTGGGT CGCTATTGGC GA |
| in silico PCR Fragment |
0001 TGACTGCAGG CAGGCACTGG AGCTACGAC- AGCCTCAAAC AAATCGCCAT TGATGGATGC
0061 ACCGAGAACG TGGAGCAGTT GAAACTAATT TTTGTCGAAG AGATTGGTTC GCGACGCAGA
0121 TCGGATTGCA TACGAACCAT CGAGGATTTA ATTGACTGCC TGGAACGAGC GGACGAACTA
0181 TCGGAGTACA ATGTGGAGCC ACTGCGCC-G GATATCAGGC AATATGCCAC AGCTCATCGA
0241 GGCTCTGAGC GCCTACACAA AGCCGGAAAA CATTCTTGGA CATCCGGTTA ATCTGTACCA
0301 GGAACTCCGA TTGGCCGAGG AACTTCGTCA GCAGCTCCGT ATTGCTCCAG CTTCCCAGAA
0361 TGCTCAGCCA TCAGTTTCGG AATTAGCTGC CGCTGTGCCT CCAACAGCAA TCCAAAACTA
0421 TGCCACCCCA GCTGCTTTTA CGGATCACAA ACGAACGATG GTCTTTAAGA AAATATCCGA
0481 GGAACTGGGT CGCTATTGGC GA |
| Assemble Data |
12297R-1.IR_full 1 TGACTGCAGGCAGGCACTGGAGCTACGACAAGCCTCAAACAAATCGCCATTGATGGATGC 60
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
12297R-1.in silico 1 TGACTGCAGGCAGGCACTGGAGCTACGAC-AGCCTCAAACAAATCGCCATTGATGGATGC 60
12297R-1.IR_full 61 ACCGAGAACGTGGAGCAGTTGAAACTAATTTTTGTCGAAGAGATTGGTTCGCGACGCAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12297R-1.in silico 61 ACCGAGAACGTGGAGCAGTTGAAACTAATTTTTGTCGAAGAGATTGGTTCGCGACGCAGA 120
12297R-1.IR_full 121 TCGGATTGCATACGAACAATCGAGGATTTAATTGACTGCCTGGAACGAGCGGACGAACTG 180
||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
12297R-1.in silico 121 TCGGATTGCATACGAACCATCGAGGATTTAATTGACTGCCTGGAACGAGCGGACGAACTA 180
12297R-1.IR_full 181 TCGGAGTACAATGTTGAGCCACTGCGCCNGGATGTCAGGCAATATGCCACAGCTCATCGA 240
|||||||||||||| ||||||||||||| |||| ||||||||||||||||||||||||||
12297R-1.in silico 181 TCGGAGTACAATGTGGAGCCACTGCGCC-GGATATCAGGCAATATGCCACAGCTCATCGA 240
12297R-1.IR_full 241 GGCTCTGAGCGCCTACACACCGCCGGAAAACATTCTTGGACATCCGGTTAATCTGTACCA 300
||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
12297R-1.in silico 241 GGCTCTGAGCGCCTACACAAAGCCGGAAAACATTCTTGGACATCCGGTTAATCTGTACCA 300
12297R-1.IR_full 301 GGAACTACGATTGGCCGAGGAACTTCGTCAGCAACTCCGGATTGCTCCAGCTTCCCAGAA 360
|||||| |||||||||||||||||||||||||| ||||| ||||||||||||||||||||
12297R-1.in silico 301 GGAACTCCGATTGGCCGAGGAACTTCGTCAGCAGCTCCGTATTGCTCCAGCTTCCCAGAA 360
12297R-1.IR_full 361 TGCTCAGCCATCAGTTTCCGAATNNGNTGCCGCTGTGCCTCCAACAGCAATCCAAAACTA 420
|||||||||||||||||| |||| | |||||||||||||||||||||||||||||||||
12297R-1.in silico 361 TGCTCAGCCATCAGTTTCGGAATTAGCTGCCGCTGTGCCTCCAACAGCAATCCAAAACTA 420
12297R-1.IR_full 421 TGCCACCCCAGCTGCTTTTACGGATCACAAACGAACGATGGTCTTTAAGAAAATATCCGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12297R-1.in silico 421 TGCCACCCCAGCTGCTTTTACGGATCACAAACGAACGATGGTCTTTAAGAAAATATCCGA 480
12297R-1.IR_full 481 GGAACTGGGTCGCTATTGGCGA 502
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12297R-1.in silico 481 GGAACTGGGTCGCTATTGGCGA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_142749.2 |
CG12297-RA (BG4), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_137891.1 |
CG3695-RA (MED23), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079208.2 |
CG4633-RA (Aats-ala-m), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141697.1 |
CG9461-RA (CG9461), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132514.2 |
CG1837-RA (CG1837), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169265.1 |
CG8866-RA, transcript variant A (CG8866), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141625.2 |
CG8866-RB, transcript variant B (CG8866), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_137417.1 |
CG10931-RA (CG10931), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_169907.1 |
CG5460-RA, transcript variant A (H), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_079694.2 |
CG5460-RC, transcript variant C (H), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135331.1 |
CG7367-RA (CG7367), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141368.2 |
CG1151-RA (Osi6), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080139.3 |
CG8171-RA (dup), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142298.2 |
CG14901-RA (Gr89a), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140112.1 |
CG14165-RA (CG14165), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NR_002486.1 |
CG14165-RA (CG14165), mRNA, miscRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_140429.3 |
CG6661-RA (CG6661), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_169901.2 |
CG4241-RB, transcript variant B (att-ORFA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_206522.1 |
CG4241-RC, transcript variant C (att-ORFA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_206523.1 |
CG33488-RA, transcript variant A (att-ORFB), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_142634.2 |
CG4241-RA, transcript variant A (att-ORFA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_137538.1 |
CG15100-RA (CG15100), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_137324.1 |
CG15614-RA (CG15614), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_136976.1 |
CG17577-RA (Cyp9h1), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_170646.3 |
CG31349-RE, transcript variant E (pyd), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_080093.2 |
CG17888-RE, transcript variant E (Pdp1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168234.1 |
CG17888-RA, transcript variant A (Pdp1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168236.1 |
CG17888-RG, transcript variant G (Pdp1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168235.1 |
CG17888-RB, transcript variant B (Pdp1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_136776.2 |
CG7686-RA (CG7686), mRNA |
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