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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12284R-2 |
| Symbol |
th |
Full Name |
thread |
| CG No |
CG12284 |
Old CG No |
CG12284 |
| Synonyms |
DIAP1, DIAPI, DIAP-1, diap1, Diap-1, IAP, DIAP1,, CG12284, dIAP1, DIAP I, Diap1, D-IAP1, DIAP, diap1/thread, Diap-1/Th, diap-1, dIAP, D-iap1, dIAP-1, l(3)j5C8, l(3)S030605b, 0736/01, 1065/03, l(3)72Dc, Iap1, E(rpr)3-1, l(3)S030605, l(3)S106503, th, IAP1, dIap1 |
| Accession No (Link to NCBI) |
NM_079377.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Wada Y, Ohsawa S, Igaki T.<br> Yorkie ensures robust tissue growth in Drosophila ribosomal protein mutants.<br> Development (2021) 148(14)
[
PubMed ID = 34313318
]
[
RRC reference
]
Wada Y, Ohsawa S, Igaki T.<br> Yorkie ensures robust tissue growth in Drosophila ribosomal protein mutants.<br> Development (2021)
[
PubMed ID = 34228792
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Clavier A, Baillet A, Rincheval-Arnold A, Coléno-Costes A, Lasbleiz C, Mignotte B, Guénal I.<br> The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA.<br> Cell Death Dis (2014) 5(9) e1405
[
PubMed ID = 25188515
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.<br> In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.<br> PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.<br> IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.<br> Curr Biol (2006) 16(15) 1531-7
[
PubMed ID = 16887350
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.<br> Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.<br> Cell Death Differ (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGCATCTGTTGTAGCTGATC |
| Primer Seq. 3' |
aatctagaggtaccGCCAATCGACTGAATTAGCTG |
| Predicted Fragment Size |
497 |
| IR fragment full Seq |
0001 TGGCATCTGT TGTAGCTGAT CTTCCATCTT ATGGACCTAT CGCTTTTGAT CAGGTGGATA
0061 ACAACACGAA CGCGACCCAG CTATTCAAAA ATAATATAAA CAAAACCAGA ATGAACGATT
0121 TAAACCGCGA GGAGACGCGA TTAAAGACCT TCACCGACTG GCCGCTGGAC TGGCTGGATA
0181 AACGCCAATT GGCCCAAACC GGCATGTACT TCACACACGC CGGCGACAAA GTTAAATGCT
0241 TTTTCTGCGG CGTGGAAATC GATTGCTGGG AGCAGGAGGA TCAGCCCGTG CCGGAACATC
0301 AGCGATGGTC GCCCAACTGT CCACTGTTGC GCCGGCGCAC TACCAACAAT GTGCCGATCA
0361 ATGCCGAAGC ATTAGATCGC ATCCTGCCGC CAATAAGCTA CGATATCTGC GGCGCCAACG
0421 ACTCGACGCT GGAGATGAGG GAGCATGCCT ACGCAGAAGG CGTCATACCC ATGTCGCAGC
0481 TAATTCAGTC GATTGGC |
| in silico PCR Fragment |
0001 TGGCATCTGT TGTAGCTGAT CTTCCGTCTT ATGGACCTAT CGCTTTTGAT CAGGTGGATA
0061 ACAACACGAA CGCGACCCAG CTATTCAAAA ATAATATAAA CAAAACCAGA ATGAACGATT
0121 TAAACCGCGA GGAGACGCGA TTAAAGACCT TCACCGACTG GCCGCTGGAC TGGCTGGATA
0181 AACGCCAATT GGCCCAAACC GGCATGTACT TCACACACGC CGGCGACAAA GTTAAATGCT
0241 TTTTCTGCGG CGTGGAAATC GGTTGCTGGG AGCAGGAGGA TCAGCCCGTG CCGGAACATC
0301 AGCGATGGTC GCCCAACTGT CCACTGTTGC GCCGGCGCAC TACCAACAAT GTGCCGATCA
0361 ATGCCGAAGC ATTAGATCGC ATCCTGCCGC CAATAAGCTA CGATATCTGC GGCGCCAACG
0421 ACTCGACGCT GGAGATGAGG GAGCATGCCT ACGCAGAAGG CGTCATACCC ATGTCGCAGC
0481 TAATTCAGTC GATTGGC |
| Assemble Data |
<PRE>
12284R-2.IR_full 1 TGGCATCTGTTGTAGCTGATCTTCC<span class="snp"><tt>A</tt></span>TCTTATGGACCTATCGCTTTTGATCAGGTGGATA 60
|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||
12284R-2.in silico 1 TGGCATCTGTTGTAGCTGATCTTCC<span class="snp"><tt>G</tt></span>TCTTATGGACCTATCGCTTTTGATCAGGTGGATA 60
12284R-2.IR_full 61 ACAACACGAACGCGACCCAGCTATTCAAAAATAATATAAACAAAACCAGAATGAACGATT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 61 ACAACACGAACGCGACCCAGCTATTCAAAAATAATATAAACAAAACCAGAATGAACGATT 120
12284R-2.IR_full 121 TAAACCGCGAGGAGACGCGATTAAAGACCTTCACCGACTGGCCGCTGGACTGGCTGGATA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 121 TAAACCGCGAGGAGACGCGATTAAAGACCTTCACCGACTGGCCGCTGGACTGGCTGGATA 180
12284R-2.IR_full 181 AACGCCAATTGGCCCAAACCGGCATGTACTTCACACACGCCGGCGACAAAGTTAAATGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 181 AACGCCAATTGGCCCAAACCGGCATGTACTTCACACACGCCGGCGACAAAGTTAAATGCT 240
12284R-2.IR_full 241 TTTTCTGCGGCGTGGAAATCG<span class="snp"><tt>A</tt></span>TTGCTGGGAGCAGGAGGATCAGCCCGTGCCGGAACATC 300
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 241 TTTTCTGCGGCGTGGAAATCG<span class="snp"><tt>G</tt></span>TTGCTGGGAGCAGGAGGATCAGCCCGTGCCGGAACATC 300
12284R-2.IR_full 301 AGCGATGGTCGCCCAACTGTCCACTGTTGCGCCGGCGCACTACCAACAATGTGCCGATCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 301 AGCGATGGTCGCCCAACTGTCCACTGTTGCGCCGGCGCACTACCAACAATGTGCCGATCA 360
12284R-2.IR_full 361 ATGCCGAAGCATTAGATCGCATCCTGCCGCCAATAAGCTACGATATCTGCGGCGCCAACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 361 ATGCCGAAGCATTAGATCGCATCCTGCCGCCAATAAGCTACGATATCTGCGGCGCCAACG 420
12284R-2.IR_full 421 ACTCGACGCTGGAGATGAGGGAGCATGCCTACGCAGAAGGCGTCATACCCATGTCGCAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12284R-2.in silico 421 ACTCGACGCTGGAGATGAGGGAGCATGCCTACGCAGAAGGCGTCATACCCATGTCGCAGC 480
12284R-2.IR_full 481 TAATTCAGTCGATTGGC 497
|||||||||||||||||
12284R-2.in silico 481 TAATTCAGTCGATTGGC 497
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
479 |
0 |
0 |
0 |
NM_168644.1 |
CG12284-RB, transcript variant B (th), mRNA |
| 100 |
479 |
0 |
0 |
0 |
NM_079377.2 |
CG12284-RA, transcript variant A (th), mRNA |
| 100 |
479 |
0 |
0 |
0 |
NM_168645.1 |
CG12284-RC, transcript variant C (th), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_136140.2 |
CG13082-RA (CG13082), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_141602.2 |
CG11982-RA (CG11982), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_139894.1 |
CG8492-RA (CG8492), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142391.3 |
CG7660-RB, transcript variant B (pxt), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169776.1 |
CG7660-RA, transcript variant A (pxt), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_137873.1 |
CG13530-RA (CG13530), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_139588.3 |
CG12605-RB, transcript variant B (CG12605), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_206275.2 |
CG12605-RA, transcript variant A (CG12605), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_142690.1 |
CG7009-RA (CG7009), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_140673.3 |
CG32171-RB, transcript variant B (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206388.1 |
CG32171-RG, transcript variant G (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168819.1 |
CG15881-RA, transcript variant A (CG15881), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140893.2 |
CG15881-RB, transcript variant B (CG15881), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206387.1 |
CG32171-RH, transcript variant H (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_170635.1 |
CG32171-RD, transcript variant D (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206389.1 |
CG32171-RF, transcript variant F (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168694.1 |
CG32171-RC, transcript variant C (Lmpt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140676.3 |
CG32171-RA, transcript variant A (Lmpt), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_206524.1 |
CG17269-RA (Fancd2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165396.1 |
CG31619-RA, transcript variant A (CG31619), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001042909.1 |
CG31619-RC, transcript variant C (CG31619), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165397.1 |
CG31619-RB, transcript variant B (CG31619), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031910.1 |
CG11566-RA (CG11566), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031911.1 |
CG33670-RA (CG33670), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_170430.2 |
CG31037-RA (ca), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_168182.1 |
CG10077-RB, transcript variant B (CG10077), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_079730.2 |
CG6669-RA (klg), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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