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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12208R-2 
 Symbol Hr46  Full Name Hormone receptor-like in 46 
 CG No CG33183  Old CG No CG12208 
 Synonyms DHR3, CG12208, CG11823, NR1F4, HR3, 2.4, l(2)k09242, Dhr3, D, dHR3, Hr3, unnamed, 12.6, CG33183, l(2)46CFi, Hr46, Hormone receptor-like in 46, Hormone receptor 3, Complementation group D 
 Accession No (Link to NCBI) NM_176121.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication]
Montagne J, Lecerf C, Parvy JP, Bennion JM, Radimerski T, Ruhf ML, Zilbermann F, Vouilloz N, Stocker H, Hafen E, Kozma SC, Thomas G.
The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila.
PLoS Genet. (2010) 6(5) e1000937 [ PubMed ID = 20463884 ] [ RRC reference ]

Cáceres L, Necakov AS, Schwartz C, Kimber S, Roberts IJ, Krause HM.
Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75.
Genes Dev. (2011) 25(14) 1476-85 [ PubMed ID = 21715559 ] [ RRC reference ]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev. Biol. (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57 [ PubMed ID = 20813879 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCAAATCCTGGCCAACCAGCCC 
 Primer Seq. 3'
aatctagaggtaccCTGGCCATGTTCTTGCACACG 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CAAATCCTGG CCAACCAGCC CATCATCGTC AAGATCGAGC CCACGCAATC CTTCCACATC 
0061 GTGGACGAGG GCGACACGAG AGTACTGAGC TTGCCCCTCA GCGATGCTGA NTAAGCTGGG 
0121 CGCCNAGTTG GATAGATCTC AAGGATATTG CCGGCCTACA NGGNCGNTGG CGGAGCCACC 
0181 TTGCTGGACG TNCTGCTTCG AGCAGGCCAA CGAGGACGGC ACCATCATAG CCACCGTGCA 
0241 GCCGGACTTG GAAAACGAGC TCGAGGCTGA GCTAAAGGCG GAGGGCGAGC CCGAGGNATG 
0301 AGACTGAANC CAGAGCCTCC GGCGCCCAAG AGATTGGCCA CCACCAGGCC AGCACAGTCG 
0361 CGGCCACAAC AGCAGCAGCA ACAGCAGCAG CAGCAGGTGA AATTCCTATC GGATCCACCG 
0421 GCATTGGCGC GCTCCAGCAG CTTCAGCAGT CTTAGCAGCT TTAGCAGCAT CAGCAATATC 
0481 AGTTCCGTGT GCAAGAACAT GGCCAG 
 in silico PCR Fragment
0001 CAAATCCTGG CCAACCAGCC CATCATCGTC AAGATCGAGC CCACGCAATC CTTCCACATC 
0061 GTGGACGAGG GCGACACGAG AGTACTGAGC TTGCCCCTCA GCGATGCTGA -TAAGCTGGG 
0121 CGCC-AGTTG GATAGATCTC AAGGATATTG CCGGCCTACA -GGCCGGTGG CGGAGCCACC 
0181 TTGCTGGACG T-CTGCTTCG AGCAGGCCAA CGAGGACGGC ACCATCATAG CCACCGTGCA 
0241 GCCGGACTTG GAAAACGAGC TCGAGGCTGA GCTAAAGGCG GAGGGCGAGC CCGAGG-ATG 
0301 AGACTGAA-C CAGAGCCTCC GGCGCCCAAG AGATTGGCGA CCACCAGGCC AGCACAGTCG 
0361 CGGCCACAAC AGCAGCAGCA ACAGCAGCAG CAGCAGGTGA AATTCCTATC GGATCCACCG 
0421 GCATTGGCAC GCTCCAGCAG CTTCAGCAGT CTCAGCAGCT TTAGCAGCAT CAGCAATATC 
0481 AGTTCCGTGT GCAAGAACAT GGCCAG 
 Assemble Data

12208R-2.IR_full       1   CAAATCCTGGCCAACCAGCCCATCATCGTCAAGATCGAGCCCACGCAATCCTTCCACATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12208R-2.in silico     1   CAAATCCTGGCCAACCAGCCCATCATCGTCAAGATCGAGCCCACGCAATCCTTCCACATC 60


12208R-2.IR_full       61  GTGGACGAGGGCGACACGAGAGTACTGAGCTTGCCCCTCAGCGATGCTGANTAAGCTGGG 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
12208R-2.in silico     61  GTGGACGAGGGCGACACGAGAGTACTGAGCTTGCCCCTCAGCGATGCTGA-TAAGCTGGG 120


12208R-2.IR_full       121 CGCCNAGTTGGATAGATCTCAAGGATATTGCCGGCCTACANGGNCGNTGGCGGAGCCACC 180
                           |||| ||||||||||||||||||||||||||||||||||| || || |||||||||||||
12208R-2.in silico     121 CGCC-AGTTGGATAGATCTCAAGGATATTGCCGGCCTACA-GGCCGGTGGCGGAGCCACC 180


12208R-2.IR_full       181 TTGCTGGACGTNCTGCTTCGAGCAGGCCAACGAGGACGGCACCATCATAGCCACCGTGCA 240
                           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
12208R-2.in silico     181 TTGCTGGACGT-CTGCTTCGAGCAGGCCAACGAGGACGGCACCATCATAGCCACCGTGCA 240


12208R-2.IR_full       241 GCCGGACTTGGAAAACGAGCTCGAGGCTGAGCTAAAGGCGGAGGGCGAGCCCGAGGNATG 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
12208R-2.in silico     241 GCCGGACTTGGAAAACGAGCTCGAGGCTGAGCTAAAGGCGGAGGGCGAGCCCGAGG-ATG 300


12208R-2.IR_full       301 AGACTGAANCCAGAGCCTCCGGCGCCCAAGAGATTGGCCACCACCAGGCCAGCACAGTCG 360
                           |||||||| ||||||||||||||||||||||||||||| |||||||||||||||||||||
12208R-2.in silico     301 AGACTGAA-CCAGAGCCTCCGGCGCCCAAGAGATTGGCGACCACCAGGCCAGCACAGTCG 360


12208R-2.IR_full       361 CGGCCACAACAGCAGCAGCAACAGCAGCAGCAGCAGGTGAAATTCCTATCGGATCCACCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12208R-2.in silico     361 CGGCCACAACAGCAGCAGCAACAGCAGCAGCAGCAGGTGAAATTCCTATCGGATCCACCG 420


12208R-2.IR_full       421 GCATTGGCGCGCTCCAGCAGCTTCAGCAGTCTTAGCAGCTTTAGCAGCATCAGCAATATC 480
                           |||||||| ||||||||||||||||||||||| |||||||||||||||||||||||||||
12208R-2.in silico     421 GCATTGGCACGCTCCAGCAGCTTCAGCAGTCTCAGCAGCTTTAGCAGCATCAGCAATATC 480


12208R-2.IR_full       481 AGTTCCGTGTGCAAGAACATGGCCAG 506
                           ||||||||||||||||||||||||||
12208R-2.in silico     481 AGTTCCGTGTGCAAGAACATGGCCAG 506

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  45  150  NM_176121.1  CG33183-RC, transcript variant C (Hr46), mRNA 
12.65   61  525  1040  1809  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
12.65   61  525  1040  1809  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
12.65   61  525  1040  1809  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
6.22   30  179  456  879  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
6.22   30  179  456  879  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
5.6   27  86  347  599  NM_139493.2  CG2083-RA (CG2083), mRNA 
5.6   27  61  141  300  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
5.6   27  61  141  300  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
5.6   27  59  187  389  NM_079507.2  CG2530-RA (corto), mRNA 
5.39   26  83  266  365  NM_167000.1  CG32778-RA (CG32778), mRNA 
5.18   25  47  228  529  NM_132403.3  CG17255-RA, transcript variant A (CG17255), mRNA 
5.18   25  47  228  529  NM_167231.3  CG17255-RB, transcript variant B (CG17255), mRNA 
4.97   24  52  284  496  NM_080364.3  CG5461-RA, transcript variant A (bun), mRNA 
4.97   24  48  260  446  NM_001042894.1  CG5461-RF, transcript variant F (bun), mRNA 
4.97   24  38  184  313  NM_132004.2  CG4136-RA (CG4136), mRNA 
4.97   24  37  118  182  NM_057511.3  CG3936-RA (N), mRNA 
4.77   23  55  183  401  NM_135785.1  CG6043-RD, transcript variant D (CG6043), mRNA 
4.77   23  55  183  400  NM_165034.1  CG6043-RA, transcript variant A (CG6043), mRNA 
4.77   23  55  183  400  NM_165037.1  CG6043-RC, transcript variant C (CG6043), mRNA 
4.77   23  55  183  400  NM_165035.1  CG6043-RB, transcript variant B (CG6043), mRNA 
4.56   22  128  306  600  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
4.56   22  128  306  600  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
4.56   22  91  261  398  NM_134474.4  CG32532-RA (CG32532), mRNA 
4.35   21  120  306  622  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
4.35   21  90  311  546  NM_078797.2  CG13109-RA (tai), mRNA 
4.14   20  83  250  390  NM_143575.2  CG12071-RA, transcript variant A (CG12071), mRNA 
4.14   20  83  250  390  NM_176592.1  CG12071-RB, transcript variant B (CG12071), mRNA 
4.14   20  70  181  268  NM_080487.2  CG10572-RA (Cdk8), mRNA 
4.14   20  14  84  154  NM_078933.2  CG11194-RA (Hey), mRNA 

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