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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12082R-2

Symbol

CG12082

Full Name

CG12082

CG No

CG12082

Old CG No

CG12082

Synonyms

CG12082

Accession No (Link to NCBI)

NM_139516.1

Inserted Chr.

Insertional Mutation

mosaic eye color

Phenotype induced by Act5C-GAL4 at 28 degrees

larval lethal

Map Viewer

Reference
[Please submit your publication]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K. Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila. PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Fernández-Espartero CH, Rizzo A, Fulford AD, Falo-Sanjuan J, Goutte-Gattat D, Ribeiro PS. Prp8 regulates oncogene-induced hyperplastic growth in Drosophila. Development (2018) 145(22) [ PubMed ID = 30333215 ] [ RRC reference ]

Kovács L, Nagy O, Pál M, Udvardy A, Popescu O, Deák P. Role of the deubiquitylating enzyme DmUsp5 in coupling ubiquitin equilibrium to development and apoptosis in Drosophila melanogaster. PLoS One (2015) 10(3) e0120875 [ PubMed ID = 25806519 ] [ RRC reference ]

Zhang J, Liu M, Su Y, Du J, Zhu AJ. A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling. G3 (Bethesda) (2012) 2(12) 1563-75 [ PubMed ID = 23275879 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGACGAGTGCGTCTACTCCTAT

Primer Seq. 3'

aatctagaggtaccTTATCCAGCTGCTGCAGGTTA

Predicted Fragment Size

IR fragment full Seq

0001 NACGAGTGCG TCTACTCCTA TGACAATCCC GAGACGCCCA CCGGTTTGTA CGTGTGCCTG 
0061 CACAGCTTTT TGGGCTTCGG CGAGGCGTAT GTGCGGGAGT ATGCCGACAA GACCGGCAAC 
0121 CGGGTCTTCC TCCACATCCA GCGGGTGAAG ACGATCAAGG AGGGCGCCGA CATGGAGGCC 
0181 GAATGCGCGG AGTCGGAGGC AGGACCGGAA CGGAAGATCA CCCGCCTGGC CATTGGCGTC 
0241 GAGGGCGGCT ACAACGAGTC GGACATGGCC AAGAAGTACG AGATCAAGGA CACGTACAGC 
0301 ATCGTGGTGG CTCCGCACCT GGATAAGAAG CTGCCCTATC CGGATCCAGA GCTGCCCATG 
0361 CGTGTCACCC AGTCGGTGGA AGCGATCCTG GCCGCCGATT CGGCAATTGC CAAGTTGGAG 
0421 AAGGCCACGC TGATGGGCAC CTGGGATGGT GAAGTGCGTC AGGCGTCGAA GTACGCAGAT 
0481 AACCTGCAGC AGCTGGATAA

in silico PCR Fragment

0001 GACGAGTGCG TCTACTCCTA TGACAATCCC GAGACGCCCA CCGGTTTGTA CGTGTGCCTG 
0061 CACAGCTTTT TGGGCTTCGG CGAGGCGTAT GTGCGGGAGT ATGCCGACAA GACCGGCAAC 
0121 CGGGTCTTCC TCCACATCCA GCGGGTGAAG ACGATCAAGG AGGGCGCCGA CATGGAGGCC 
0181 GAATGCGCGG AGTCGGAGGC AGGACCGGAA CGGAAGATCA CCCGCCTGGC CATTGGCGTC 
0241 GAGGGCGGCT ACAACGAGTC GGACATGGCC AAGAAGTACG AGATCAAGGA CACGTACAGC 
0301 ATCGTGGTGG CTCCGCACCT GGATAAGAAG CTGCCCTATC CGGATCCAGA GCTGCCCATG 
0361 CGTGTCACCC AGTCGGTGGA AGCGATCCTG GCCGCCGACT CGGCAATTGC CAAGTTGGAG 
0421 AAGGCCACGC TGATGGGCAC CTGGGATGGT GAAGTGCGTC AGGCGTCGAA GTACGCAGAT 
0481 AACCTGCAGC AGCTGGATAA

Assemble Data

<PRE>

12082R-2.IR_full        1   ACGAGTGCGTCTACTCCTATGACAATCCCGAGACGCCCACCGGTTTGTACGTGTGCCTG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico      1   ACGAGTGCGTCTACTCCTATGACAATCCCGAGACGCCCACCGGTTTGTACGTGTGCCTG 59


12082R-2.IR_full       61  CACAGCTTTTTGGGCTTCGGCGAGGCGTATGTGCGGGAGTATGCCGACAAGACCGGCAAC 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     61  CACAGCTTTTTGGGCTTCGGCGAGGCGTATGTGCGGGAGTATGCCGACAAGACCGGCAAC 119


12082R-2.IR_full       121 CGGGTCTTCCTCCACATCCAGCGGGTGAAGACGATCAAGGAGGGCGCCGACATGGAGGCC 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     121 CGGGTCTTCCTCCACATCCAGCGGGTGAAGACGATCAAGGAGGGCGCCGACATGGAGGCC 179


12082R-2.IR_full       181 GAATGCGCGGAGTCGGAGGCAGGACCGGAACGGAAGATCACCCGCCTGGCCATTGGCGTC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     181 GAATGCGCGGAGTCGGAGGCAGGACCGGAACGGAAGATCACCCGCCTGGCCATTGGCGTC 239


12082R-2.IR_full       241 GAGGGCGGCTACAACGAGTCGGACATGGCCAAGAAGTACGAGATCAAGGACACGTACAGC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     241 GAGGGCGGCTACAACGAGTCGGACATGGCCAAGAAGTACGAGATCAAGGACACGTACAGC 299


12082R-2.IR_full       301 ATCGTGGTGGCTCCGCACCTGGATAAGAAGCTGCCCTATCCGGATCCAGAGCTGCCCATG 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     301 ATCGTGGTGGCTCCGCACCTGGATAAGAAGCTGCCCTATCCGGATCCAGAGCTGCCCATG 359


12082R-2.IR_full       361 CGTGTCACCCAGTCGGTGGAAGCGATCCTGGCCGCCGA<span class="snp"><tt>T</tt></span>TCGGCAATTGCCAAGTTGGAG 419
                           ||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
12082R-2.in silico     361 CGTGTCACCCAGTCGGTGGAAGCGATCCTGGCCGCCGA<span class="snp"><tt>C</tt></span>TCGGCAATTGCCAAGTTGGAG 419


12082R-2.IR_full       421 AAGGCCACGCTGATGGGCACCTGGGATGGTGAAGTGCGTCAGGCGTCGAAGTACGCAGAT 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12082R-2.in silico     421 AAGGCCACGCTGATGGGCACCTGGGATGGTGAAGTGCGTCAGGCGTCGAAGTACGCAGAT 479


12082R-2.IR_full       481 AACCTGCAGCAGCTGGATAA 499
                           ||||||||||||||||||||
12082R-2.in silico     481 AACCTGCAGCAGCTGGATAA 499

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	8	NM_139516.1	CG12082-RA (CG12082), mRNA
0	0	4	4	2	NM_135667.3	CG6614-RA (CG6614), mRNA
0	0	4	3	3	NM_057414.3	CG1389-RA (tor), mRNA
0	0	3	5	3	NM_132740.1	CG15890-RA (CG15890), mRNA
0	0	2	3	3	NM_206483.1	CG15890-RA (CG15890), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA
0	0	2	3	3	NM_206482.1	CG15890-RA (CG15890), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA
0	0	2	3	3	NM_142073.3	CG15890-RA (CG15890), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA, sub-group O CG3143-RA, transcript variant A (foxo), mRNA
0	0	2	3	2	NM_136876.2	CG8321-RA (CG8321), mRNA
0	0	2	1	1	NM_205985.1	CG32975-RC, transcript variant C (nAcRalpha-34E), mRNA
0	0	2	1	1	NM_205986.1	CG32975-RB, transcript variant B (nAcRalpha-34E), mRNA
0	0	2	1	1	NM_176035.2	CG32975-RA, transcript variant A (nAcRalpha-34E), mRNA
0	0	1	4	6	NM_169264.1	CG9735-RA, transcript variant A (Aats-trp), mRNA
0	0	1	4	5	NM_057303.4	CG7831-RA (ncd), mRNA
0	0	1	3	6	NM_079659.2	CG3937-RA, transcript variant A (cher), mRNA
0	0	1	3	6	NM_206502.1	CG3937-RD, transcript variant D (cher), mRNA
0	0	1	3	2	NM_132090.2	CG3446-RA (CG3446), mRNA
0	0	1	2	7	NM_206558.1	CG33340-RA (CG33340), mRNA
0	0	1	2	6	NM_140571.3	CG5895-RA (CG5895), mRNA
0	0	1	2	3	NM_057977.3	CG9239-RA, transcript variant A (B4), mRNA
0	0	1	2	3	NM_165046.1	CG9239-RB, transcript variant B (B4), mRNA
0	0	1	2	3	NM_132328.1	CG17446-RA (CG17446), mRNA
0	0	1	2	3	NM_132441.1	CG2174-RA, transcript variant A (Myo10A), mRNA
0	0	1	2	3	NM_001042804.1	CG2174-RB, transcript variant B (Myo10A), mRNA
0	0	1	2	2	NM_206381.1	CG16838-RC, transcript variant C (CG16838), mRNA
0	0	1	2	2	NM_206380.1	CG16838-RD, transcript variant D (CG16838), mRNA
0	0	1	2	2	NM_136672.2	CG1688-RA (CG1688), mRNA
0	0	0	6	3	NM_141308.1	CG12170-RA (CG12170), mRNA
0	0	0	6	3	NM_132199.2	CG2206-RA, transcript variant A (l(1)G0193), mRNA
0	0	0	5	11	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	0	5	6	NM_170004.1	CG7070-RB, transcript variant B (PyK), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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