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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12074R-1 |
| Symbol |
CG31004 |
Full Name |
CG31004 |
| CG No |
CG31004 |
Old CG No |
CG12074 |
| Synonyms |
CG15560, CT5150, CG12074, anon-WO0140519.233, CG31004 |
| Accession No (Link to NCBI) |
NM_170539.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Qin P, Wang Q, Wu Y, You Q, Li M, Guo Z.<br> Age mosaic of gut epithelial cells prevents aging.<br> Nat Commun (2025) 16(1) 6734
[
PubMed ID = 40695820
]
[
RRC reference
]
Jonusaite S, Beyenbach KW, Meyer H, Paululat A, Izumi Y, Furuse M, Rodan AR.<br> The septate junction protein Mesh is required for epithelial morphogenesis, ion transport, and paracellular permeability in the <i>Drosophila</i> Malpighian tubule.<br> Am J Physiol Cell Physiol (2020) 318(3) C675-C694
[
PubMed ID = 31913700
]
[
RRC reference
]
Izumi Y, Furuse K, Furuse M.<br> Septate junctions regulate gut homeostasis through regulation of stem cell proliferation and enterocyte behavior in <i>Drosophila</i>.<br> J Cell Sci (2019) 132(18)
[
PubMed ID = 31444286
]
[
RRC reference
]
Izumi Y, Yanagihashi Y, Furuse M.<br> A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut.<br> J Cell Sci (2012) 125(Pt 20) 4923-33
[
PubMed ID = 22854041
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTGGGAGCGGCAGCATGGAAA |
| Primer Seq. 3' |
aatctagaggtaccGATAGGCCACACAATCCTGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GTGGGAGCGG CAGCATGGAA AGCGATGGGC GCGGGCTCTC TGCGATAATT GGATTCGCGC
0061 CGACCGATTC CTCAGAAACT TTGCGGCCGA CCTGCCACTT TGCCCCTGCA CCTTGGACCA
0121 GGCTGTCCTG GACAANAGGA CGCTTCCGCC CTGACCGCGA GTGCGACAAG GACTCGAACC
0181 CTANGCTGCC TGCGTCATCG CGGGGCCNAT CCACTGCGTG GTCAGTGGAA CGCCAGTTGC
0241 CCAAGGAGCT GAACAGCAGT GCTGCTACGA TCGTTATGGC TTCCTGATGC TGACCTACGA
0301 CCAAATGTGG GGCTCTCGTC CCCGTCGCGT CCACAANCCT GGGCAAGATG CCCTGGAACG
0361 AGGCCAGCAA GGTGCCCTCG CTGTCCATGT GGTTCCACGA CATGCGCCCC TTCTACTCCT
0421 GCTGCTACTG GCAGGAGGAG CAGGCGGTGG GCTGCGAAAC CTACCGCTTC GAGCGTCGTC
0481 CCTCGCAGGA TTGTGTGGcC TATC |
| in silico PCR Fragment |
0001 GTGGGAGCGG CAGCATGGAA AGCGATGGGC GCGGGCTCTC TGCGATAATT GGATTCGCGC
0061 CGACCGATTC CTCAGGAACT TTGCGGCCGA CCTGCCACTT TGCCCCTGCA CCTTGGACCA
0121 GGCTGTCCTG GACAA-AGGA CGCTTCCGCC CTGACCGCGA GTGCGACAAG GACTCGAACC
0181 CTA-GCTGCC TGCGTCATCG CGGGGCC-AT CCACTGCGTG GTCAGTGGAA CGCCAGTTGC
0241 CCAAGGAGCT GAACAGCAGT GCTGCTACGA TCGTTATGGC TTCCTGATGC TGACCTACGA
0301 CCAAATGTGG GGCTCTCGTC CCCGTCGCGT CCACAA-CCT GGGCAAGATG CCCTGGAACG
0361 AGGCCAGCAA GGTGCCCTCG CTGTCCATGT GGTTCCACGA CATGCGCCCC TTCTACTCCT
0421 GCTGCTACTG GCAGGAGGAG CAGGCGGTGG GCTGCGAAAC CTACCGCTTC GAGCGTCGTC
0481 CCTCGCAGGA TTGTGTGGCC TATC |
| Assemble Data |
<PRE>
12074R-1.IR_full 1 GTGGGAGCGGCAGCATGGAAAGCGATGGGCGCGGGCTCTCTGCGATAATTGGATTCGCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 1 GTGGGAGCGGCAGCATGGAAAGCGATGGGCGCGGGCTCTCTGCGATAATTGGATTCGCGC 60
12074R-1.IR_full 61 CGACCGATTCCTCAG<span class="snp"><tt>A</tt></span>AACTTTGCGGCCGACCTGCCACTTTGCCCCTGCACCTTGGACCA 120
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 61 CGACCGATTCCTCAG<span class="snp"><tt>G</tt></span>AACTTTGCGGCCGACCTGCCACTTTGCCCCTGCACCTTGGACCA 120
12074R-1.IR_full 121 GGCTGTCCTGGACAA<span class="snp"><tt>N</tt></span>AGGACGCTTCCGCCCTGACCGCGAGTGCGACAAGGACTCGAACC 180
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 121 GGCTGTCCTGGACAA<span class="snp"><tt>-</tt></span>AGGACGCTTCCGCCCTGACCGCGAGTGCGACAAGGACTCGAACC 180
12074R-1.IR_full 181 CTA<span class="snp"><tt>N</tt></span>GCTGCCTGCGTCATCGCGGGGCC<span class="snp"><tt>N</tt></span>ATCCACTGCGTGGTCAGTGGAACGCCAGTTGC 240
|||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
12074R-1.in silico 181 CTA<span class="snp"><tt>-</tt></span>GCTGCCTGCGTCATCGCGGGGCC<span class="snp"><tt>-</tt></span>ATCCACTGCGTGGTCAGTGGAACGCCAGTTGC 240
12074R-1.IR_full 241 CCAAGGAGCTGAACAGCAGTGCTGCTACGATCGTTATGGCTTCCTGATGCTGACCTACGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 241 CCAAGGAGCTGAACAGCAGTGCTGCTACGATCGTTATGGCTTCCTGATGCTGACCTACGA 300
12074R-1.IR_full 301 CCAAATGTGGGGCTCTCGTCCCCGTCGCGTCCACAA<span class="snp"><tt>N</tt></span>CCTGGGCAAGATGCCCTGGAACG 360
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
12074R-1.in silico 301 CCAAATGTGGGGCTCTCGTCCCCGTCGCGTCCACAA<span class="snp"><tt>-</tt></span>CCTGGGCAAGATGCCCTGGAACG 360
12074R-1.IR_full 361 AGGCCAGCAAGGTGCCCTCGCTGTCCATGTGGTTCCACGACATGCGCCCCTTCTACTCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 361 AGGCCAGCAAGGTGCCCTCGCTGTCCATGTGGTTCCACGACATGCGCCCCTTCTACTCCT 420
12074R-1.IR_full 421 GCTGCTACTGGCAGGAGGAGCAGGCGGTGGGCTGCGAAACCTACCGCTTCGAGCGTCGTC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12074R-1.in silico 421 GCTGCTACTGGCAGGAGGAGCAGGCGGTGGGCTGCGAAACCTACCGCTTCGAGCGTCGTC 480
12074R-1.IR_full 481 CCTCGCAGGATTGTGTGGCCTATC 504
||||||||||||||||||||||||
12074R-1.in silico 481 CCTCGCAGGATTGTGTGGCCTATC 504
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_170539.1 |
CG31004-RA, transcript variant A (CG31004), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_170540.1 |
CG31004-RB, transcript variant B (CG31004), mRNA |
| 0.62 |
3 |
3 |
2 |
3 |
NM_143555.2 |
CG9717-RA (CG9717), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_080158.2 |
CG8669-RA, transcript variant A (crc), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_001038841.1 |
CG8669-RD, transcript variant D (crc), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_176331.1 |
CG33209-RA (comm3), mRNA |
| 0 |
0 |
2 |
7 |
14 |
NM_001038966.1 |
CG33967-RA (CG33967), mRNA |
| 0 |
0 |
2 |
7 |
10 |
NM_142180.2 |
CG4285-RA (CG4285), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134761.2 |
CG10869-RA (CG10869), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135432.1 |
CG13108-RA (CG13108), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079086.2 |
CG5821-RA (qkr58E-2), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_169197.2 |
CG31187-RA (CG31187), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_079818.2 |
CG10002-RA (fkh), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001014565.1 |
CG10491-RB, transcript variant B (vn), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_079218.2 |
CG10491-RA, transcript variant A (vn), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135247.2 |
CG9138-RA (SP1070), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169142.1 |
CG10272-RB, transcript variant B (gpp), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_136955.2 |
CG8815-RA, transcript variant A (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165916.1 |
CG8815-RC, transcript variant C (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165915.1 |
CG8815-RB, transcript variant B (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165917.1 |
CG8815-RD, transcript variant D (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169144.1 |
CG10272-RC, transcript variant C (gpp), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169143.1 |
CG10272-RD, transcript variant D (gpp), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_141398.1 |
CG10272-RA, transcript variant A (gpp), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132056.3 |
CG4790-RA (fs(1)M3), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164931.1 |
CG31719-RB, transcript variant B (RluA-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164930.1 |
CG31719-RA, transcript variant A (RluA-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164932.1 |
CG31719-RC, transcript variant C (RluA-1), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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