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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12072R-1 |
| Symbol |
wts |
Full Name |
warts |
| CG No |
CG12072 |
Old CG No |
CG12072 |
| Synonyms |
Lats/Warts, Warts/Lats, lats, Dlats, Warts, dmLATS, LATS, lts, WTS/LATS, CG12072, warts/lats, Lats, l(3)100Aa, wts, wart, Wts/Lats, wts/lats, warts, Wts |
| Accession No (Link to NCBI) |
NM_170524.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Turkel N, Sahota VK, Bolden JE, Goulding KR, Doggett K, Willoughby LF, Blanco E, Martin-Blanco E, Corominas M, Ellul J, Aigaki T, Richardson HE, Brumby AM.
The BTB-zinc finger transcription factor abrupt acts as an epithelial oncogene in Drosophila melanogaster through maintaining a progenitor-like cell state.
PLoS Genet. (2013) 9(7) e1003627
[
PubMed ID = 23874226
]
[
RRC reference
]
Vissers JH, Manning SA, Kulkarni A, Harvey KF.
A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.
Nat Commun (2016) 7 10368
[
PubMed ID = 26758424
]
[
RRC reference
]
Enomoto M, Kizawa D, Ohsawa S, Igaki T.
JNK signaling is converted from anti- to pro-tumor pathway by Ras-mediated switch of Warts activity.
Dev. Biol. (2015) 403(2) 162-71
[
PubMed ID = 25967126
]
[
RRC reference
]
Fernández BG, Gaspar P, Brás-Pereira C, Jezowska B, Rebelo SR, Janody F.
Actin-Capping Protein and the Hippo pathway regulate F-actin and tissue growth in Drosophila.
Development (2011) 138(11) 2337-46
[
PubMed ID = 21525075
]
[
RRC reference
]
Doggett K, Grusche FA, Richardson HE, Brumby AM.
Loss of the Drosophila cell polarity regulator Scribbled promotes epithelial tissue overgrowth and cooperation with oncogenic Ras-Raf through impaired Hippo pathway signaling.
BMC Dev. Biol. (2011) 11 57
[
PubMed ID = 21955824
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCAGCGGGCGAAAAAAGGGG |
| Primer Seq. 3' |
aatctagaggtaccGCTGGTGGGTGTGGTGCGAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCCAGCGGGC GAAAAAAAGG gcGGTCGCCC CAATGATAAA TACACGGCGG AAGCCCTCGA
0061 GAGCATCAAG CAGGACCTAA CCCGATTTGA AGTACAAAAT AACCATAGGA ATAATCAGAA
0121 TTACACACCT CTGCGATACA CGGCGACCAA CGGACGCAAC GATGCACTTA CTCCTGACTA
0181 TCACCACGCC AAGCAGCCGA TGGAGCTGCC ACCCTCCGCC TCTCCTGCTC CGGACGTGGT
0241 CATACCGCCG CCGCCCGCCA TTGTAGGTCA GCCCGGAGCC GGCTCCATAT CCGTATCCGG
0301 TGTGGGCGTT GGAGTGGTGG GTGTGGCGAA CGGACGTGTG CCAAAGATGA TGACGGCCCT
0361 AATGCCAAAC AAACTGATCC GGAAGCCGAG CATCGAACGG GACACGGCGA GCAGTCACTA
0421 CCTGCGCTGC AGTCCGGCTC TGGACTCCGG AGCCGGTAGC TCCCGATCGG ACAGCCCCCA
0481 TTCGCACCAC ACCCACCAGC |
| in silico PCR Fragment |
0001 TCCAGCGGGC GAAAAAAGGG GCGGTCGCCC CAATGATAAA TACACGGCGG AAGCCCTCGA
0061 GAGCATCAAG CAGGACCTAA CCCGATTTGA AGTACAAAAT AACCATAGGA ATAATCAGAA
0121 TTACACACCT CTGCGATACA CGGCGACCAA CGGACGCAAC GATGCACTTA CTCCTGACTA
0181 TCACCACGCC AAGCAGCCGA TGGAGCCGCC ACCCTCCGCC TCTCCTGCTC CGGACGTGGT
0241 CATACCGCCG CCGCCCGCCA TTGTAGGTCA GCCCGGAGCC GGCTCCATAT CCGTATCCGG
0301 TGTGGGCGTT GGAGTGGTGG GTGTGGCGAA CGGACGTGTG CCAAAGATGA TGACGGCCCT
0361 AATGCCAAAC AAACTGATCC GGAAGCCGAG CATCGAACGG GACACGGCGA GCAGTCACTA
0421 CCTGCGCTGC AGTCCGGCTC TGGACTCCGG AGCCGGTAGC TCCCGATCGG ACAGCCCCCA
0481 TTCGCACCAC ACCCACCAGC |
| Assemble Data |
12072R-1.IR_full 1 TCCAGCGGGCGAAAAAAAGGGCGGTCGCCCCAATGATAAATACACGGCGGAAGCCCTCGA 60
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 1 TCCAGCGGGCGAAAAAAGGGGCGGTCGCCCCAATGATAAATACACGGCGGAAGCCCTCGA 60
12072R-1.IR_full 61 GAGCATCAAGCAGGACCTAACCCGATTTGAAGTACAAAATAACCATAGGAATAATCAGAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 61 GAGCATCAAGCAGGACCTAACCCGATTTGAAGTACAAAATAACCATAGGAATAATCAGAA 120
12072R-1.IR_full 121 TTACACACCTCTGCGATACACGGCGACCAACGGACGCAACGATGCACTTACTCCTGACTA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 121 TTACACACCTCTGCGATACACGGCGACCAACGGACGCAACGATGCACTTACTCCTGACTA 180
12072R-1.IR_full 181 TCACCACGCCAAGCAGCCGATGGAGCTGCCACCCTCCGCCTCTCCTGCTCCGGACGTGGT 240
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
12072R-1.in silico 181 TCACCACGCCAAGCAGCCGATGGAGCCGCCACCCTCCGCCTCTCCTGCTCCGGACGTGGT 240
12072R-1.IR_full 241 CATACCGCCGCCGCCCGCCATTGTAGGTCAGCCCGGAGCCGGCTCCATATCCGTATCCGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 241 CATACCGCCGCCGCCCGCCATTGTAGGTCAGCCCGGAGCCGGCTCCATATCCGTATCCGG 300
12072R-1.IR_full 301 TGTGGGCGTTGGAGTGGTGGGTGTGGCGAACGGACGTGTGCCAAAGATGATGACGGCCCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 301 TGTGGGCGTTGGAGTGGTGGGTGTGGCGAACGGACGTGTGCCAAAGATGATGACGGCCCT 360
12072R-1.IR_full 361 AATGCCAAACAAACTGATCCGGAAGCCGAGCATCGAACGGGACACGGCGAGCAGTCACTA 420
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12072R-1.in silico 361 AATGCCAAACAAACTGATCCGGAAGCCGAGCATCGAACGGGACACGGCGAGCAGTCACTA 420
12072R-1.IR_full 421 CCTGCGCTGCAGTCCGGCTCTGGACTCCGGAGCCGGTAGCTCCCGATCGGACAGCCCCCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12072R-1.in silico 421 CCTGCGCTGCAGTCCGGCTCTGGACTCCGGAGCCGGTAGCTCCCGATCGGACAGCCCCCA 480
12072R-1.IR_full 481 TTCGCACCACACCCACCAGC 500
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12072R-1.in silico 481 TTCGCACCACACCCACCAGC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
7 |
NM_170524.1 |
CG12072-RA (wts), mRNA |
| 0.2 |
1 |
2 |
4 |
3 |
NM_143241.1 |
CG5484-RC, transcript variant C (CG5484), mRNA |
| 0.2 |
1 |
2 |
4 |
3 |
NM_170284.1 |
CG5484-RA, transcript variant A (CG5484), mRNA |
| 0.2 |
1 |
2 |
4 |
3 |
NM_170285.1 |
CG5484-RB, transcript variant B (CG5484), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_141780.2 |
CG4674-RA (CG4674), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_136834.1 |
CG13204-RA, transcript variant A (CG13204), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_165827.1 |
CG13204-RB, transcript variant B (CG13204), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_136188.2 |
CG10949-RA (CG10949), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132724.2 |
CG1810-RA (mRNA-capping-enzyme), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166873.1 |
CG3638-RB, transcript variant B (CG3638), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166872.1 |
CG3638-RD, transcript variant D (CG3638), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_130541.3 |
CG3638-RC, transcript variant C (CG3638), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166874.1 |
CG3638-RA, transcript variant A (CG3638), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_167685.1 |
CG12701-RB, transcript variant B (CG12701), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_134512.4 |
CG12701-RA, transcript variant A (CG12701), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_001043139.1 |
CG11282-RC, transcript variant C (caps), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_057412.3 |
CG10223-RA (Top2), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078647.3 |
CG9907-RA, transcript variant A (para), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042815.1 |
CG9907-RB, transcript variant B (para), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042816.1 |
CG9907-RC, transcript variant C (para), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_130604.2 |
CG17766-RA (CG17766), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164773.1 |
CG7123-RB, transcript variant B (LanB1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057270.3 |
CG7123-RA, transcript variant A (LanB1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NR_001953.1 |
CR32028, miscRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168232.2 |
CG32369-RA, transcript variant A (CG32369), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132766.2 |
CG9053-RA, transcript variant A (CG9053), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078847.2 |
CG7595-RB, transcript variant B (ck), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165099.1 |
CG7595-RA, transcript variant A (ck), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167427.1 |
CG9053-RB, transcript variant B (CG9053), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_142474.1 |
CG14304-RA (CG14304), mRNA |
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