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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12019R-2

Symbol

Cdc37

Full Name

Cdc37

CG No

CG12019

Old CG No

CG12019

Synonyms

cdc37, DROCPDR, CPDR-2, CPDR-1, E(sev)3B, CG12019, Cdc37

Accession No (Link to NCBI)

NM_057658.4

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Lee CW, Kwon YC, Lee Y, Park MY, Choe KM. cdc37 is essential for JNK pathway activation and wound closure in Drosophila. Mol Biol Cell (2019) 30(21) 2651-2658 [ PubMed ID = 31483695 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGTGGACTACAGCAAGTGG

Primer Seq. 3'

aatctagaggtaccGAATCGTCGTACTTGCGCAGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGGTGGACT ACAGCAAGTG GAAGAACATC GAGATTTCGG ATGATGAGGA CGACACTCAC 
0061 CCGAACATAG ACACGCCTTC TCTGTTCCGC TGGCGGCACC AGGCGCGCGT GGANGCGCAT 
0121 GGNCGGNAGA TGGACCACGA GAAGGACGAG CTTAAGAAAA AGCGCCAGAG CTACCAGGCG 
0181 CGCCNTCATG GACGTCAAGG AGCGGATCTC AAAGAAGGAT GGCGACGAGG AGGCCCTGAA 
0241 GAAAGAGCTC GAAAAGATCG AGGCCGAGGG CAAAGAGCTG GACCGCATCG AGAGCGAGAT 
0301 GATTAAGAAG GAAAAGAAAA CACCCTGGAA CGTGGACACC ATCAGCAAGC CCGGCTTTGA 
0361 GAAGACCGTA ATCAACAAGA AGGCCGGCCG CAAGCCGGAT NGAGAATCTG TCCGAGGAAG 
0421 AGCGAGAGCA GCGCATGAAG CAATTCGTCA AGGAGAACGA GAAGCTCTGC CAACAGTATG 
0481 GCATGCTGCG CAAGTACGAC GATTC

in silico PCR Fragment

0001 ATGGTGGACT ACAGCAAGTG GAAGAACATC GAGATTTCGG ATGATGAGGA CGACACTCAC 
0061 CCAAACATAG ACACGCCTTC TCTGTTCCGC TGGCGGCACC AGGCGCGCGT GGA-GCGCAT 
0121 GG-CGG-AGA TGGACCACGA GAAGGACGAG CTTAAGAAAA AGCGCCAGAG CTACCAGGCG 
0181 CGCC-TCATG GACGTCAAGG AGCGGATCTC AAAGAAGGAT GGCGACGAGG AGGCCCTGAA 
0241 GAAAGAGCTC GAAAAGATCG AGGCCGAGGG CAAAGAGCTG GACCGCATCG AGAGCGAGAT 
0301 GATTAAGAAG GAAAAGAAAA CACCCTGGAA CGTGGACACC ATCAGCAAGC CCGGCTTTGA 
0361 GAAGACCGTA ATCAACAAGA AGGCCGGCCG CAAGCCGGAT -GAGAATCTG TCCGAGGAAG 
0421 AGCGAGAGCA GCGCATGAAG CAATTCGTCA AGGAGAACGA GAAGCTCTGC CAGCAGTATG 
0481 GCATGCTGCG CAAGTACGAC GATTC

Assemble Data

<PRE>

12019R-2.IR_full       1   ATGGTGGACTACAGCAAGTGGAAGAACATCGAGATTTCGGATGATGAGGACGACACTCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12019R-2.in silico     1   ATGGTGGACTACAGCAAGTGGAAGAACATCGAGATTTCGGATGATGAGGACGACACTCAC 60


12019R-2.IR_full       61  CC<span class="snp"><tt>G</tt></span>AACATAGACACGCCTTCTCTGTTCCGCTGGCGGCACCAGGCGCGCGTGGA<span class="snp"><tt>N</tt></span>GCGCAT 120
                           ||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
12019R-2.in silico     61  CC<span class="snp"><tt>A</tt></span>AACATAGACACGCCTTCTCTGTTCCGCTGGCGGCACCAGGCGCGCGTGGA<span class="snp"><tt>-</tt></span>GCGCAT 120


12019R-2.IR_full       121 GG<span class="snp"><tt>N</tt></span>CGG<span class="snp"><tt>N</tt></span>AGATGGACCACGAGAAGGACGAGCTTAAGAAAAAGCGCCAGAGCTACCAGGCG 180
                           ||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
12019R-2.in silico     121 GG<span class="snp"><tt>-</tt></span>CGG<span class="snp"><tt>-</tt></span>AGATGGACCACGAGAAGGACGAGCTTAAGAAAAAGCGCCAGAGCTACCAGGCG 180


12019R-2.IR_full       181 CGCC<span class="snp"><tt>N</tt></span>TCATGGACGTCAAGGAGCGGATCTCAAAGAAGGATGGCGACGAGGAGGCCCTGAA 240
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
12019R-2.in silico     181 CGCC<span class="snp"><tt>-</tt></span>TCATGGACGTCAAGGAGCGGATCTCAAAGAAGGATGGCGACGAGGAGGCCCTGAA 240


12019R-2.IR_full       241 GAAAGAGCTCGAAAAGATCGAGGCCGAGGGCAAAGAGCTGGACCGCATCGAGAGCGAGAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12019R-2.in silico     241 GAAAGAGCTCGAAAAGATCGAGGCCGAGGGCAAAGAGCTGGACCGCATCGAGAGCGAGAT 300


12019R-2.IR_full       301 GATTAAGAAGGAAAAGAAAACACCCTGGAACGTGGACACCATCAGCAAGCCCGGCTTTGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12019R-2.in silico     301 GATTAAGAAGGAAAAGAAAACACCCTGGAACGTGGACACCATCAGCAAGCCCGGCTTTGA 360


12019R-2.IR_full       361 GAAGACCGTAATCAACAAGAAGGCCGGCCGCAAGCCGGAT<span class="snp"><tt>N</tt></span>GAGAATCTGTCCGAGGAAG 420
                           ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
12019R-2.in silico     361 GAAGACCGTAATCAACAAGAAGGCCGGCCGCAAGCCGGAT<span class="snp"><tt>-</tt></span>GAGAATCTGTCCGAGGAAG 420


12019R-2.IR_full       421 AGCGAGAGCAGCGCATGAAGCAATTCGTCAAGGAGAACGAGAAGCTCTGCCA<span class="snp"><tt>A</tt></span>CAGTATG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
12019R-2.in silico     421 AGCGAGAGCAGCGCATGAAGCAATTCGTCAAGGAGAACGAGAAGCTCTGCCA<span class="snp"><tt>G</tt></span>CAGTATG 480


12019R-2.IR_full       481 GCATGCTGCGCAAGTACGACGATTC 505
                           |||||||||||||||||||||||||
12019R-2.in silico     481 GCATGCTGCGCAAGTACGACGATTC 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057658.4	CG12019-RA (Cdc37), mRNA
0	0	8	2	6	NM_141198.3	CG9776-RA, transcript variant A (CG9776), mRNA
0	0	3	5	4	NM_135095.1	CG14014-RA (CG14014), mRNA
0	0	3	2	3	NM_143030.2	CG18410-RA (CG18410), mRNA
0	0	3	2	3	NM_166996.2	CG32776-RA, transcript variant A (CG32776), mRNA
0	0	3	2	3	NM_001038741.1	CG32776-RC, transcript variant C (CG32776), mRNA
0	0	3	2	3	NM_206622.2	CG32776-RB, transcript variant B (CG32776), mRNA
0	0	3	2	3	NM_001038740.1	CG32776-RD, transcript variant D (CG32776), mRNA
0	0	2	4	5	NM_170361.1	CG5514-RA, transcript variant A (CG5514), mRNA
0	0	2	4	5	NM_143342.1	CG5514-RB, transcript variant B (CG5514), mRNA
0	0	2	4	3	NM_143315.1	CG5612-RA (CG5612), mRNA
0	0	2	4	2	NM_079613.2	CG7642-RA (ry), mRNA
0	0	1	5	3	NM_142812.1	CG13843-RA (CG13843), mRNA
0	0	1	4	7	NM_143731.4	CG9473-RA, transcript variant A (MED6), mRNA
0	0	1	4	7	NM_169310.2	CG9473-RB, transcript variant B (MED6), mRNA
0	0	1	4	3	NM_141341.2	CG1208-RA (CG1208), mRNA
0	0	1	4	2	NM_141758.3	CG6544-RA, transcript variant A (fau), mRNA
0	0	1	3	4	NM_167995.1	CG32281-RA (CG32281), mRNA
0	0	1	3	4	NM_130524.2	CG3658-RA (CDC45L), mRNA
0	0	1	3	3	NM_079497.2	CG9768-RA (hkb), mRNA
0	0	1	3	3	NM_132596.1	CG4004-RA, transcript variant A (CG4004), mRNA
0	0	1	3	2	NM_206420.1	CG33291-RA (CG33291), mRNA
0	0	1	3	2	NM_141556.3	CG9773-RA (CG9773), mRNA
0	0	1	2	5	NM_206581.1	CG2304-RC, transcript variant C (Trc8), mRNA
0	0	1	2	5	NM_170413.2	CG2304-RA, transcript variant A (Trc8), mRNA
0	0	1	2	5	NM_170414.1	CG2304-RB, transcript variant B (Trc8), mRNA
0	0	1	2	5	NM_206580.1	CG2304-RD, transcript variant D (Trc8), mRNA
0	0	1	2	4	NM_142313.2	CG10326-RA (CG10326), mRNA
0	0	1	2	4	NM_138189.2	CG6905-RA (CG6905), mRNA
0	0	1	2	3	NM_130625.2	CG3981-RB, transcript variant B (Unc-76), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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