National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11859R-2 
 Symbol CG11859  Full Name CG11859 
 CG No CG11859  Old CG No CG11859 
 Synonyms CG11859 
 Accession No (Link to NCBI) NM_143108.1 
 Inserted Chr.
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Hasygar K, Hietakangas V.
p53- and ERK7-dependent ribosome surveillance response regulates Drosophila insulin-like peptide secretion.
PLoS Genet. (2014) 10(11) e1004764 [ PubMed ID = 25393288 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol. (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGTGCTGCGATATCTCACCAAA 
 Primer Seq. 3'
aatctagaggtaccGGAAACCCACGATCATAGAGT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 GTGCTNCGAT ATCTCACCAA AGAGGACTTC CGCGTCCTCA CCGCCATCGA GATGGGCATG 
0061 AAGAACCACG AGCTGGTCCC TGGTCCCCTG GCTGCTGCCA TTGCGAATCT GAAGTCGGGT 
0121 GGAGTCCACA AGCTGCTCAA GGAGCTGTGC AAGCACAAGC TATTGGCCTA CGAACGGGGA 
0181 AAGAAATATG ATGGCTACCG CTTGACAAAC ACGGGCTACG ACTATCTGGC TCTGAAGTCT 
0241 CTCACACTGC GGGGATCGGT CAGTTCCTTT GGCAACCAGA TTGGAATTGG CAAGGAGTCC 
0301 AACATCTACG TGGTGGCTGA TGAGGAGGGC ACTCCAATTT GCCTCAAACT ACACCGATTG 
0361 GGTCGAACTT GTTTCAGGAA TGTAAAAGCC AAAAGAGACT ACCACGGTCG CCGACACAAG 
0421 GCCTCCTGGT TGTACTTATC TCGCATCTCG GCTACCCGCG AGTTTGCCTA CATGTCGGCA 
0481 CTCTATGATC GTGGGTTTCC  
 in silico PCR Fragment
0001 GTGCTGCGAT ATCTCACCAA AGAGGACTTC CGCGTCCTCA CCGCCATCGA GATGGGCATG 
0061 AAGAACCACG AGCTGGTCCC TGGTCCCCTG GCTGCTGCCA TTGCGAATCT GAAGTCGGGT 
0121 GGAGTCCACA AGCTGCTCAA GGAGCTGTGC AAGCACAAGC TATTGGCCTA CGAACGGGGA 
0181 AAGAAATATG ATGGCTACCG CTTGACAAAC ACGGGCTACG ACTATCTGGC TCTGAAGTCT 
0241 CTCACACTGC GGGGATCGGT CAGTTCCTTT GGCAACCAGA TTGGAATTGG CAAGGAGTCC 
0301 AACATCTACG TGGTGGCTGA TGAGGAGGGC ACTCCAATTT GCCTCAAACT ACACCGATTG 
0361 GGTCGAACTT GTTTCAGGAA TGTAAAAGCC AAAAGAGACT ACCACGGTCG CCGACACAAG 
0421 GCCTCCTGGT TGTACTTATC TCGCATCTCG GCTACCCGCG AGTTTGCCTA CATGTCGGCA 
0481 CTCTATGATC GTGGGTTTCC  
 Assemble Data

11859R-2.IR_full       1   GTGCTNCGATATCTCACCAAAGAGGACTTCCGCGTCCTCACCGCCATCGAGATGGGCATG 60
                           ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     1   GTGCTGCGATATCTCACCAAAGAGGACTTCCGCGTCCTCACCGCCATCGAGATGGGCATG 60


11859R-2.IR_full       61  AAGAACCACGAGCTGGTCCCTGGTCCCCTGGCTGCTGCCATTGCGAATCTGAAGTCGGGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     61  AAGAACCACGAGCTGGTCCCTGGTCCCCTGGCTGCTGCCATTGCGAATCTGAAGTCGGGT 120


11859R-2.IR_full       121 GGAGTCCACAAGCTGCTCAAGGAGCTGTGCAAGCACAAGCTATTGGCCTACGAACGGGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     121 GGAGTCCACAAGCTGCTCAAGGAGCTGTGCAAGCACAAGCTATTGGCCTACGAACGGGGA 180


11859R-2.IR_full       181 AAGAAATATGATGGCTACCGCTTGACAAACACGGGCTACGACTATCTGGCTCTGAAGTCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     181 AAGAAATATGATGGCTACCGCTTGACAAACACGGGCTACGACTATCTGGCTCTGAAGTCT 240


11859R-2.IR_full       241 CTCACACTGCGGGGATCGGTCAGTTCCTTTGGCAACCAGATTGGAATTGGCAAGGAGTCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     241 CTCACACTGCGGGGATCGGTCAGTTCCTTTGGCAACCAGATTGGAATTGGCAAGGAGTCC 300


11859R-2.IR_full       301 AACATCTACGTGGTGGCTGATGAGGAGGGCACTCCAATTTGCCTCAAACTACACCGATTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     301 AACATCTACGTGGTGGCTGATGAGGAGGGCACTCCAATTTGCCTCAAACTACACCGATTG 360


11859R-2.IR_full       361 GGTCGAACTTGTTTCAGGAATGTAAAAGCCAAAAGAGACTACCACGGTCGCCGACACAAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     361 GGTCGAACTTGTTTCAGGAATGTAAAAGCCAAAAGAGACTACCACGGTCGCCGACACAAG 420


11859R-2.IR_full       421 GCCTCCTGGTTGTACTTATCTCGCATCTCGGCTACCCGCGAGTTTGCCTACATGTCGGCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11859R-2.in silico     421 GCCTCCTGGTTGTACTTATCTCGCATCTCGGCTACCCGCGAGTTTGCCTACATGTCGGCA 480


11859R-2.IR_full       481 CTCTATGATCGTGGGTTTCC 500
                           ||||||||||||||||||||
11859R-2.in silico     481 CTCTATGATCGTGGGTTTCC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_143108.1  CG11859-RA (CG11859), mRNA 
0.41   NM_136699.3  CG12128-RA, transcript variant A (CG12128), mRNA 
0.41   NM_206068.1  CG12128-RB, transcript variant B (CG12128), mRNA 
0.2   NM_001038900.1  CG11583-RA (CG11583), mRNA 
0.2   NM_165031.1  CG31728-RA (CG31728), mRNA 
0.2   NM_164658.1  CG11147-RB, transcript variant B (CG11147), mRNA 
0.2   NM_135110.1  CG11147-RA, transcript variant A (CG11147), mRNA 
0   NM_140706.1  CG6497-RA (CG6497), mRNA 
0   NM_168811.1  CG32209-RB (CG32209), mRNA 
0   NM_142601.1  CG4845-RA (CG4845), mRNA 
0   NM_079729.2  CG4792-RA (Dcr-1), mRNA 
0   NM_079395.2  CG9695-RA (Dab), mRNA 
0   NM_142215.2  CG5205-RA (CG5205), mRNA 
0   NM_001014545.1  CG33519-RB (Unc-89), mRNA 
0   NM_135192.2  CG9542-RA (CG9542), mRNA 
0   NM_143041.1  CG10899-RA, transcript variant A (CG10899), mRNA 
0   NM_170174.1  CG10899-RB, transcript variant B (CG10899), mRNA 
0   NM_166335.2  CG7097-RA, transcript variant A (CG7097), mRNA 
0   NM_137555.2  CG7097-RB, transcript variant B (CG7097), mRNA 
0   NM_143745.2  CG18389-RA (Eip93F), mRNA 
0   NM_135335.2  CG7424-RA (RpL36A), mRNA 
0   NM_168629.2  CG32149-RC, transcript variant C (RhoGAP71E), mRNA 
0   NM_168630.1  CG32149-RB, transcript variant B (RhoGAP71E), mRNA 
0   NM_140527.2  CG32149-RA, transcript variant A (RhoGAP71E), mRNA 
0   NM_143759.2  CG6760-RA (l(3)70Da), mRNA 
0   NM_078805.3  CG5838-RA (Dref), mRNA 
0   NM_079419.2  CG4059-RB, transcript variant B (ftz-f1), mRNA 
0   NM_141564.2  CG8145-RA (CG8145), mRNA 
0   NM_001031917.1  CG1414-RB, transcript variant B (bbx), mRNA 
0   NM_001031918.1  CG1410-RA, transcript variant A (waw), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.