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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11093R-3 |
| Symbol |
CG11093 |
Full Name |
CG11093 |
| CG No |
CG11093 |
Old CG No |
CG11093 |
| Synonyms |
CG11093 |
| Accession No (Link to NCBI) |
NM_143689.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Fischer S, Bayersdorfer F, Harant E, Reng R, Arndt S, Bosserhoff AK, Schneuwly S.
fussel (fuss)--A negative regulator of BMP signaling in Drosophila melanogaster.
PLoS One (2012) 7(8) e42349
[
PubMed ID = 22879948
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCTGTACGGAGTGCAGATTG |
| Primer Seq. 3' |
aatctagaggtaccACGCCAGGAATTAAAGTTCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGCTGTACGG AGTGCAGATT GTATCCCTGC ACATTGAAGG TCAAGAGCGC CTTTGCTTGG
0061 CGCAAATTTC AAATACATTA CTAAAACAAT TCAGTTACAA CGAGATACAC AACAGGAGAG
0121 TTGCTCTTGG CATTACTTGT GTACAATGTA CACCCGTGCA ATTGGAAATA TTGAGAAGAG
0181 CTGGCGCCAT GCCAGTGAGT TCCCGACGAT GTGGCATGAT TACCCGCAGG GAAGCGGAGC
0241 GTCTTATGCA AAAGTTTCTT GGGCGACAAT ACACCACCAC GACTACCTGA CGACTTTGCG
0301 TTTAACGTNC CAGCACAAAT GTGCTTGGGG CTGCCGCGGA TCCTTTCTAC CCTCGCGTTA
0361 CAACTCATCA AGGGCTAAGT GCATTAAGTG TTCATATTGC GGAATGTTTT TCTCGCCCAA
0421 TAAATTTATT TTTCACTCAC ATCGCATAAC AACTAATGAC AGGTACGTTC AGCCTGATGC
0481 AGCGAACTTT AATTCCTGGC GT |
| in silico PCR Fragment |
0001 TGCTGTACGG AGTGCAGATT GTATCCCTGC ACATTGAAGG TCAAGAGCGC CTTTGCTTGG
0061 CGCAAATTTC AAATACATTA CTAAAACAAT TCAGTTACAA CGAGATACAC AACAGGAGAG
0121 TTGCTCTTGG CATTACTTGT GTACAATGTA CACCCGTGCA ATTGGAAATA TTGAGAAGAG
0181 CTGGCGCCAT GCCAGTGAGT TCCCGACGAT GTGGCATGAT TACCCGCAGG GAAGCGGAGC
0241 GTC-TATGCA AAAGTTTCTT GGGCGACAAT ACACCACCAC GACTACCTGA CGACTTTGCG
0301 TTTAACGT-C CAGCACAAAT GTGCTTGGGG CTGCCGCGGA TCCTTTCTAC CCTCGCGTTA
0361 CAACTCATCA AGGGCTAAGT GCATTAAGTG TTCATATTGC GGAATGTTTT TCTCGCCCAA
0421 TAAATTTATT TTTCACTCAC ATCGCATAAC AACTAATGAC AGGTACGTTC AGCCTGATGC
0481 AGCGAACTTT AATTCCTGGC GT |
| Assemble Data |
11093R-3.IR_full 1 TGCTGTACGGAGTGCAGATTGTATCCCTGCACATTGAAGGTCAAGAGCGCCTTTGCTTGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 1 TGCTGTACGGAGTGCAGATTGTATCCCTGCACATTGAAGGTCAAGAGCGCCTTTGCTTGG 60
11093R-3.IR_full 61 CGCAAATTTCAAATACATTACTAAAACAATTCAGTTACAACGAGATACACAACAGGAGAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 61 CGCAAATTTCAAATACATTACTAAAACAATTCAGTTACAACGAGATACACAACAGGAGAG 120
11093R-3.IR_full 121 TTGCTCTTGGCATTACTTGTGTACAATGTACACCCGTGCAATTGGAAATATTGAGAAGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 121 TTGCTCTTGGCATTACTTGTGTACAATGTACACCCGTGCAATTGGAAATATTGAGAAGAG 180
11093R-3.IR_full 181 CTGGCGCCATGCCAGTGAGTTCCCGACGATGTGGCATGATTACCCGCAGGGAAGCGGAGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 181 CTGGCGCCATGCCAGTGAGTTCCCGACGATGTGGCATGATTACCCGCAGGGAAGCGGAGC 240
11093R-3.IR_full 241 GTCTTATGCAAAAGTTTCTTGGGCGACAATACACCACCACGACTACCTGACGACTTTGCG 300
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 241 GTC-TATGCAAAAGTTTCTTGGGCGACAATACACCACCACGACTACCTGACGACTTTGCG 300
11093R-3.IR_full 301 TTTAACGTNCCAGCACAAATGTGCTTGGGGCTGCCGCGGATCCTTTCTACCCTCGCGTTA 360
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 301 TTTAACGT-CCAGCACAAATGTGCTTGGGGCTGCCGCGGATCCTTTCTACCCTCGCGTTA 360
11093R-3.IR_full 361 CAACTCATCAAGGGCTAAGTGCATTAAGTGTTCATATTGCGGAATGTTTTTCTCGCCCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 361 CAACTCATCAAGGGCTAAGTGCATTAAGTGTTCATATTGCGGAATGTTTTTCTCGCCCAA 420
11093R-3.IR_full 421 TAAATTTATTTTTCACTCACATCGCATAACAACTAATGACAGGTACGTTCAGCCTGATGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11093R-3.in silico 421 TAAATTTATTTTTCACTCACATCGCATAACAACTAATGACAGGTACGTTCAGCCTGATGC 480
11093R-3.IR_full 481 AGCGAACTTTAATTCCTGGCGT 502
||||||||||||||||||||||
11093R-3.in silico 481 AGCGAACTTTAATTCCTGGCGT 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_143689.1 |
CG11093-RA (CG11093), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_139554.3 |
CG12076-RA, transcript variant A (YT521-B), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167481.2 |
CG9209-RA, transcript variant A (vap), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078637.2 |
CG9209-RB, transcript variant B (vap), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206759.1 |
CG9209-RD, transcript variant D (vap), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167480.1 |
CG9209-RC, transcript variant C (vap), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132069.2 |
CG32754-RA (vanin-like), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_169068.2 |
CG1081-RA, transcript variant A (Rheb), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_132826.2 |
CG9240-RA (CG9240), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_169069.2 |
CG1081-RB, transcript variant B (Rheb), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_167870.1 |
CG9155-RA, transcript variant A (Myo61F), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_167872.1 |
CG9155-RC, transcript variant C (Myo61F), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_167871.1 |
CG9155-RB, transcript variant B (Myo61F), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_057586.3 |
CG9155-RD, transcript variant D (Myo61F), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_205947.1 |
CG3858-RB, transcript variant B (gcm2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135021.2 |
CG12194-RA (CG12194), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135458.3 |
CG3858-RA, transcript variant A (gcm2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_140276.2 |
CG6947-RA (CG6947), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_206460.1 |
CG33325-RA (CG33325), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_143596.2 |
CG12054-RA (CG12054), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_134642.2 |
CG11371-RB (dbr), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_139485.1 |
CG12187-RA (CG12187), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_139980.2 |
CG6662-RA (CG6662), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_141438.2 |
CG10068-RA (CG10068), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_142829.1 |
CG4907-RA (CG4907), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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