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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10741R-3

Symbol CG10741 Full Name CG10741

CG No CG10741 Old CG No CG10741

Synonyms NEST:bs23g10, CG10741

Accession No (Link to NCBI) NM_168555.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Jeibmann A, Halama K, Witte HT, Kim SN, Eikmeier K, Koos B, Klämbt C, Paulus W.
Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
J Neurooncol (2015) 124(3) 373-83 [ PubMed ID = 26224160 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTTGCTTACTGGATGCCTTCG

Primer Seq. 3'

aatctagaggtaccCTCTGAGTGCAACTGCAACTG

Predicted Fragment Size

IR fragment full Seq

0001 NTTGCTTACT GGATGCCTTC GAGGATTACT ACTACCACAA GGAGATCCAG CGTTTGGTCG 
0061 CCGAAACCGC TGGGGGGGCT ACAGCAACAT CTCCCGCCTC ATNCCGCCTC ATCCGCCTCC 
0121 TCGACGGCCT CCATCTCATC TGCATCCTGC TCGAGTGGAC CCTCNCACTT CCTCCATTGT 
0181 CTCGTCCGCG GCCAGTTCGC ACGGTTCGTT GGCCCAGGTG GCTACTGCCC GAGCGGCCGC 
0241 TGCTCTGGCG GACCAACAAG CACTGGCCAG cCAGAGGGCC ATGTTCTACA ATGTCCAGCA 
0301 TCCTNCAgcN GCTGGAGCAG CTGCATgNGT TGCAGGCGGA GTCCGGCAAT CAGCAGATgC 
0361 ATCCGCNGGC GAACGCCGAT CCTAACGCCT CCTCCATGGC CAACAGTNNG CTCTGGCAGC 
0421 CATGGCGAGA TCTCCAGCAG GcGGCCNCCA TGCATCACCA GCTCTACAGG CAGCAGCAGC 
0481 AGCAGTTGCA GTTGCACTCA GAG

in silico PCR Fragment

0001 GTTGCTTACT GGATGCCTTC GAGGATTACT ACTACCACAA GGAGATCCAG CGTTTGGTCG 
0061 CCGAAACCGC TGGGGGGGCT ACAGCAACAT CTCCCGCCTC AT-CCGCCTC ATCCGCCTCC 
0121 TCGACGGCCT CCATCTCATC TGCATCCTGC TCGAGTGGAC CCTC-CACTT CCTCCATTGT 
0181 CTCGTCCGCG GCCAGTTCGC ACGGTTCGTT GGCCCAGGTG GCTACTGCCC GAGCGGCCGC 
0241 TGCTCTGGCG GACCAACAAG CACTGGCCAG CCAGAGGGCC ATGTTCTACA ATGTCCAGCA 
0301 TCCT-CAGCA GCTGGAGCAG CTGCATGCGT TGCAGGCGGA GTCCGGCAAT CAGCAGATGC 
0361 ATCCGCAGGC GAACGCCGAT CCTAACGCCT CCTCCATGGC CAACAGTCTG CTCTGGCAGC 
0421 CATGGCGAGA TCTCCAGCAG GCGGCCGCCA TGCATCACCA GCTCTACAGG CAGCAGCAGC 
0481 AGCAGTTGCA GTTGCACTCA GAG

Assemble Data


10741R-3.IR_full        1   TTGCTTACTGGATGCCTTCGAGGATTACTACTACCACAAGGAGATCCAGCGTTTGGTCG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10741R-3.in silico      1   TTGCTTACTGGATGCCTTCGAGGATTACTACTACCACAAGGAGATCCAGCGTTTGGTCG 59


10741R-3.IR_full       61  CCGAAACCGCTGGGGGGGCTACAGCAACATCTCCCGCCTCATNCCGCCTCATCCGCCTCC 119
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
10741R-3.in silico     61  CCGAAACCGCTGGGGGGGCTACAGCAACATCTCCCGCCTCAT-CCGCCTCATCCGCCTCC 119


10741R-3.IR_full       121 TCGACGGCCTCCATCTCATCTGCATCCTGCTCGAGTGGACCCTCNCACTTCCTCCATTGT 179
                           |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
10741R-3.in silico     121 TCGACGGCCTCCATCTCATCTGCATCCTGCTCGAGTGGACCCTC-CACTTCCTCCATTGT 179


10741R-3.IR_full       181 CTCGTCCGCGGCCAGTTCGCACGGTTCGTTGGCCCAGGTGGCTACTGCCCGAGCGGCCGC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10741R-3.in silico     181 CTCGTCCGCGGCCAGTTCGCACGGTTCGTTGGCCCAGGTGGCTACTGCCCGAGCGGCCGC 239


10741R-3.IR_full       241 TGCTCTGGCGGACCAACAAGCACTGGCCAGCCAGAGGGCCATGTTCTACAATGTCCAGCA 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10741R-3.in silico     241 TGCTCTGGCGGACCAACAAGCACTGGCCAGCCAGAGGGCCATGTTCTACAATGTCCAGCA 299


10741R-3.IR_full       301 TCCTNCAGCNGCTGGAGCAGCTGCATGNGTTGCAGGCGGAGTCCGGCAATCAGCAGATGC 359
                           |||| |||| ||||||||||||||||| ||||||||||||||||||||||||||||||||
10741R-3.in silico     301 TCCT-CAGCAGCTGGAGCAGCTGCATGCGTTGCAGGCGGAGTCCGGCAATCAGCAGATGC 359


10741R-3.IR_full       361 ATCCGCNGGCGAACGCCGATCCTAACGCCTCCTCCATGGCCAACAGTNNGCTCTGGCAGC 419
                           |||||| ||||||||||||||||||||||||||||||||||||||||  |||||||||||
10741R-3.in silico     361 ATCCGCAGGCGAACGCCGATCCTAACGCCTCCTCCATGGCCAACAGTCTGCTCTGGCAGC 419


10741R-3.IR_full       421 CATGGCGAGATCTCCAGCAGGCGGCCNCCATGCATCACCAGCTCTACAGGCAGCAGCAGC 479
                           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
10741R-3.in silico     421 CATGGCGAGATCTCCAGCAGGCGGCCGCCATGCATCACCAGCTCTACAGGCAGCAGCAGC 479


10741R-3.IR_full       481 AGCAGTTGCAGTTGCACTCAGAG 502
                           |||||||||||||||||||||||
10741R-3.in silico     481 AGCAGTTGCAGTTGCACTCAGAG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	2	14	20	NM_168555.1	CG10741-RA, transcript variant A (CG10741), mRNA
0.82	4	6	11	46	NM_136191.2	CG31688-RA (CG31688), mRNA
0.82	4	6	11	33	NM_165331.2	CG31687-RA (CG31687), mRNA
0.82	4	2	40	180	NM_079671.2	CG7847-RA, transcript variant A (sr), mRNA
0.82	4	2	38	156	NM_169786.1	CG7847-RB, transcript variant B (sr), mRNA
0.82	4	2	26	123	NM_078514.2	CG9653-RA (brk), mRNA
0.82	4	2	23	87	NM_079321.2	CG10571-RA (ara), mRNA
0.82	4	2	11	40	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0.62	3	2	18	114	NM_143765.2	CG12218-RA (mei-P26), mRNA
0.62	3	2	9	41	NM_167990.1	CG11494-RA, transcript variant A (BtbVII), mRNA
0.62	3	2	9	41	NM_079172.2	CG11494-RB, transcript variant B (BtbVII), mRNA
0.41	2	9	15	62	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0.41	2	9	10	34	NM_167130.1	CG11387-RB, transcript variant B (ct), mRNA
0.41	2	6	2	36	NM_206458.2	CG17816-RD, transcript variant D (CG17816), mRNA
0.41	2	6	2	36	NM_169199.1	CG17816-RA, transcript variant A (CG17816), mRNA
0.41	2	6	2	36	NM_169198.1	CG17816-RB, transcript variant B (CG17816), mRNA
0.41	2	6	2	36	NM_141487.2	CG17816-RC, transcript variant C (CG17816), mRNA
0.41	2	4	38	108	NM_135954.1	CG13260-RA (CG13260), mRNA
0.41	2	3	9	41	NM_142140.1	CG17304-RA (CG17304), mRNA
0.41	2	2	8	59	NM_176037.1	CG32972-RB, transcript variant B (CG32972), mRNA
0.41	2	2	8	59	NM_205987.1	CG32972-RA, transcript variant A (CG32972), mRNA
0.41	2	2	5	30	NM_001043257.1	CG34157-RH, transcript variant H (Dys), mRNA
0.2	1	4	11	8	NM_078881.2	CG13968-RA, transcript variant A (sNPF), mRNA
0.2	1	4	11	8	NM_165316.1	CG13968-RB, transcript variant B (sNPF), mRNA
0.2	1	4	4	66	NM_176542.1	CG7029-RA (CG7029), mRNA
0.2	1	4	2	31	NM_167029.1	CG10706-RC, transcript variant C (SK), mRNA
0.2	1	4	2	31	NM_167031.1	CG10706-RA, transcript variant A (SK), mRNA
0.2	1	4	2	31	NM_167032.1	CG10706-RB, transcript variant B (SK), mRNA
0.2	1	4	2	30	NM_206631.1	CG10706-RF, transcript variant F (SK), mRNA
0.2	1	3	30	80	NM_001042804.1	CG2174-RB, transcript variant B (Myo10A), mRNA

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