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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10642R-1 |
| Symbol |
Klp64D |
Full Name |
Kinesin-like protein at 64D |
| CG No |
CG10642 |
Old CG No |
CG10642 |
| Synonyms |
Kif3A, klp64D, KIF3A, CG10642, DmKlp64D, KLP64D, KLP4, Klp4, Klp64D |
| Accession No (Link to NCBI) |
NM_079210.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kim JY, Tsogtbaatar O, Cho KO.<br> Dynein Heavy Chain 64C Differentially Regulates Cell Survival and Proliferation of Wingless-Producing Cells in <i>Drosophila melanogaster</i>.<br> J Dev Biol (2021) 9(4)
[
PubMed ID = 34698231
]
[
RRC reference
]
Vuong LT, Won JH, Nguyen MB, Choi KW.<br> Role of Armadillo repeat 2 and kinesin-II motor subunit Klp64D for wingless signaling in Drosophila.<br> Sci Rep (2020) 10(1) 13864
[
PubMed ID = 32807823
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.<br> Identification and functional analysis of healing regulators in Drosophila.<br> PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Vuong LT, Mukhopadhyay B, Choi KW.<br> Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila.<br> Development (2014) 141(16) 3222-32
[
PubMed ID = 25063455
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCCGCAGGAGGAGGCAAAT |
| Primer Seq. 3' |
aatctagaggtaccAGATCCCGCACCTCTTCGTTA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGCCGCAGG AGGAGGCAAA TGCGGGAACA ACGGCCCAAC TCGATGACGA AATCGAGAAT
0061 GTGCGTGTGG TGGTGCGAAC GCGACCCATG GACAAAAATG AGTTGTCCGC GGGAGCGCTA
0121 AGTGCGATTT CGGTGGATAA GATCAACCGG GCCATTACGG TGATGAAACC GAATGCCACC
0181 GCCAATGAAC CGCCGAAAAC GTACTACTTC GACAATGTAT TCGATGGTGG CTCCAATCAA
0241 ATGGATCTCT ATGTGGACAC CGCTCGTCCG ATTGTGGACA AGGTGCTGGA GGGCTATAAT
0301 GGCACCATCT TGGCCTACGG ACAAACGGGC ACCGGCAAGA CGTATACCAT GTCGGGGAAT
0361 CCGGATTCGC CGCAAACAAA GGGCATCATT CCGAATGCAT TTGCGCACAT CTTTGGTCAC
0421 ATTGCTAAGG CCAAGGAGAA TCAGAAGTTC CTGGTGCGCG TCAGCTACAT GGAGATCTAT
0481 AACGAAGAGG TGCGGGATCT |
| in silico PCR Fragment |
0001 ATGCCGCAGG AGGAGGCAAA TGCGGGAACA ACGGCCCAAC TCGATGACGA AATCGAGAAT
0061 GTGCGTGTGG TGGTGCGAAC GCGACCCATG GACAAAAATG AGTTGTCCGC GGGAGCGCTA
0121 AGTGCGATTT CGGTGGATAA GATCAACCGG GCCATTACGG TGATGAAACC GAATGCCACC
0181 GCCAATGAAC CGCCGAAAAC GTACTACTTC GACAATGTAT TCGATGGTGG CTCCAATCAA
0241 ATGGATCTCT ATGTGGACAC CGCTCGTCCG ATTGTGGACA AGGTGCTGGA GGGCTATAAT
0301 GGCACCATCT TGGCCTACGG ACAAACGGGC ACCGGCAAGA CGTATACCAT GTCGGGGAAT
0361 CCGGATTCGC CGCAAACAAA GGGCATCATT CCGAATGCAT TTGCGCACAT CTTTGGTCAC
0421 ATTGCTAAGG CCAAGGAGAA TCAGAAGTTC CTGGTGCGCG TCAGCTACAT GGAGATCTAT
0481 AACGAAGAGG TGCGGGATCT |
| Assemble Data |
<PRE>
10642R-1.IR_full 1 ATGCCGCAGGAGGAGGCAAATGCGGGAACAACGGCCCAACTCGATGACGAAATCGAGAAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 1 ATGCCGCAGGAGGAGGCAAATGCGGGAACAACGGCCCAACTCGATGACGAAATCGAGAAT 60
10642R-1.IR_full 61 GTGCGTGTGGTGGTGCGAACGCGACCCATGGACAAAAATGAGTTGTCCGCGGGAGCGCTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 61 GTGCGTGTGGTGGTGCGAACGCGACCCATGGACAAAAATGAGTTGTCCGCGGGAGCGCTA 120
10642R-1.IR_full 121 AGTGCGATTTCGGTGGATAAGATCAACCGGGCCATTACGGTGATGAAACCGAATGCCACC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 121 AGTGCGATTTCGGTGGATAAGATCAACCGGGCCATTACGGTGATGAAACCGAATGCCACC 180
10642R-1.IR_full 181 GCCAATGAACCGCCGAAAACGTACTACTTCGACAATGTATTCGATGGTGGCTCCAATCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 181 GCCAATGAACCGCCGAAAACGTACTACTTCGACAATGTATTCGATGGTGGCTCCAATCAA 240
10642R-1.IR_full 241 ATGGATCTCTATGTGGACACCGCTCGTCCGATTGTGGACAAGGTGCTGGAGGGCTATAAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 241 ATGGATCTCTATGTGGACACCGCTCGTCCGATTGTGGACAAGGTGCTGGAGGGCTATAAT 300
10642R-1.IR_full 301 GGCACCATCTTGGCCTACGGACAAACGGGCACCGGCAAGACGTATACCATGTCGGGGAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 301 GGCACCATCTTGGCCTACGGACAAACGGGCACCGGCAAGACGTATACCATGTCGGGGAAT 360
10642R-1.IR_full 361 CCGGATTCGCCGCAAACAAAGGGCATCATTCCGAATGCATTTGCGCACATCTTTGGTCAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 361 CCGGATTCGCCGCAAACAAAGGGCATCATTCCGAATGCATTTGCGCACATCTTTGGTCAC 420
10642R-1.IR_full 421 ATTGCTAAGGCCAAGGAGAATCAGAAGTTCCTGGTGCGCGTCAGCTACATGGAGATCTAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10642R-1.in silico 421 ATTGCTAAGGCCAAGGAGAATCAGAAGTTCCTGGTGCGCGTCAGCTACATGGAGATCTAT 480
10642R-1.IR_full 481 AACGAAGAGGTGCGGGATCT 500
||||||||||||||||||||
10642R-1.in silico 481 AACGAAGAGGTGCGGGATCT 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
4 |
NM_079210.1 |
CG10642-RA (Klp64D), mRNA |
| 0.62 |
3 |
2 |
6 |
7 |
NM_176176.1 |
CG8183-RB, transcript variant B (Khc-73), mRNA |
| 0.62 |
3 |
2 |
6 |
7 |
NM_135357.3 |
CG8183-RA, transcript variant A (Khc-73), mRNA |
| 0 |
0 |
2 |
8 |
7 |
NM_079305.2 |
CG7293-RA (Klp68D), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141195.2 |
CG9791-RA, transcript variant A (CG9791), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_142803.1 |
CG5326-RA, transcript variant A (CG5326), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_143014.2 |
CG13624-RC, transcript variant C (CG13624), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001043288.1 |
CG13624-RF, transcript variant F (CG13624), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170150.1 |
CG13624-RA, transcript variant A (CG13624), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001043287.1 |
CG13624-RE, transcript variant E (CG13624), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170151.1 |
CG13624-RB, transcript variant B (CG13624), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170152.1 |
CG13624-RD, transcript variant D (CG13624), mRNA |
| 0 |
0 |
1 |
9 |
16 |
NM_057303.4 |
CG7831-RA (ncd), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_080054.2 |
CG1560-RA (mys), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_139532.1 |
CG14967-RA (CG14967), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132704.1 |
CG11071-RA (CG11071), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079026.2 |
CG8095-RB, transcript variant B (scb), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166083.1 |
CG8095-RA, transcript variant A (scb), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142971.2 |
CG5524-RA (CG5524), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169834.1 |
CG7720-RA, transcript variant A (CG7720), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142496.2 |
CG7720-RB, transcript variant B (CG7720), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143059.1 |
CG13646-RA (CG13646), mRNA |
| 0 |
0 |
0 |
7 |
19 |
NM_080254.2 |
CG6392-RA (cmet), mRNA |
| 0 |
0 |
0 |
7 |
19 |
NM_001032076.1 |
CG33694-RA, transcript variant A (cana), mRNA |
| 0 |
0 |
0 |
7 |
19 |
NM_001032080.1 |
CG33695-RA, transcript variant A (CG33695), mRNA |
| 0 |
0 |
0 |
5 |
11 |
NM_080314.2 |
CG8590-RA (Klp3A), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_140879.1 |
CG9392-RA (CG9392), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_057550.3 |
CG10118-RB, transcript variant B (ple), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_057549.2 |
CG10118-RA, transcript variant A (ple), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04814
:
Motor proteins
|
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