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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10539R-3 |
| Symbol |
S6k |
Full Name |
RPS6-p70-protein kinase |
| CG No |
CG10539 |
Old CG No |
CG10539 |
| Synonyms |
p70/S6K, S6K, dS6K, CG10539, dS6k, ds6k, s6k11, p70 S6K, DS6K, p70[S6kinase], p70[S6 kinase], fs(3)07084, Dp70s6k, p70s6K, dps6k, dp70s6k, 7084, p70[S6k], p70S6K, Dp70[s6k], Dp70S6k, dp70[S6k], l(3)07084, S6k |
| Accession No (Link to NCBI) |
NM_079217.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Resnik-Docampo M, de Celis JF.<br> MAP4K3 is a component of the TORC1 signalling complex that modulates cell growth and viability in Drosophila melanogaster.<br> PLoS One (2011) 6(1) e14528
[
PubMed ID = 21267071
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAACTGTTCGACCTGGAGCTG |
| Primer Seq. 3' |
aatctagaggtaccATGCCCTCACGCTCCAAATGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAACTGTTCG ACCTGGAGCT GCACGACCTG GAACTGCAGG ACGACAAGGC CAGGGACTCC
0061 GACGACGACA GGATCGAGCT GGACGACGTT GACCTAGAAC CGGAATTGTG TATTAATCTG
0121 CACCAAGACA CTGAGGGCCA GGAGACCATA NCAGCTCTGC GAGGNAGAAT GTTAATCCAG
0181 GTAAAATCAA GCTGGGACCC AAGGACTTTG AGCTCAAGAA GGTCCTTGGC AAAGGTGGTT
0241 ATGGCAAAGT ATTTCAGGTG CGCAAGACCG CTGGACGAGA TGCTAACAAA TATTTTGCCA
0301 TGAAGGTGCT CAAAAAGGCA TCCATTGTGA CCAATCAAAA GGACACAGCG CACACCCGCG
0361 CCGAGCGCAA TATACTCGAG GCAGTCAAGC ATCCCTTCAT AGTGGAGCTA GTTTATGCCT
0421 TCCAGACAGA CGGAAAACTA TACCTTATAC TTGAATATCT CAGCGGTGGA GAGCTGTTCA
0481 TGCATTTGGA GCGTGAGGGC AT |
| in silico PCR Fragment |
0001 GAACTGTTCG ACCTGGAGCT GCACGACCTG GAACTGCAGG ACGACAAGGC CAGGGACTCC
0061 GACGACGACA GGATCGAGCT GGACGATGTT GACCTAGAAC CGGAATTGTG TATTAATCTG
0121 CACCAAGACA CTGAGGGCCA GGAGACCATA -CAGCTCTGC GAGG-AGAAT GTTAATCCAG
0181 GTAAAATCAA GCTGGGACCC AAGGACTTTG AGCTCAAGAA GGTCCTTGGC AAAGGTGGTT
0241 ATGGCAAAGT ATTTCAGGTG CGCAAGACCG CTGGACGAGA TGCTAACAAA TATTTTGCCA
0301 TGAAGGTGCT CAAAAAGGCA TCCATTGTGA CCAATCAAAA GGACACAGCG CACACCCGCG
0361 CCGAGCGCAA TATACTCGAG GCAGTCAAGC ATCCCTTCAT AGTGGAGCTA GTTTATGCCT
0421 TCCAGACAGA CGGAAAACTA TACCTTATAC TTGAATATCT CAGCGGTGGA GAGCTGTTCA
0481 TGCATTTGGA GCGTGAGGGC AT |
| Assemble Data |
<PRE>
10539R-3.IR_full 1 GAACTGTTCGACCTGGAGCTGCACGACCTGGAACTGCAGGACGACAAGGCCAGGGACTCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 1 GAACTGTTCGACCTGGAGCTGCACGACCTGGAACTGCAGGACGACAAGGCCAGGGACTCC 60
10539R-3.IR_full 61 GACGACGACAGGATCGAGCTGGACGA<span class="snp"><tt>C</tt></span>GTTGACCTAGAACCGGAATTGTGTATTAATCTG 120
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||
10539R-3.in silico 61 GACGACGACAGGATCGAGCTGGACGA<span class="snp"><tt>T</tt></span>GTTGACCTAGAACCGGAATTGTGTATTAATCTG 120
10539R-3.IR_full 121 CACCAAGACACTGAGGGCCAGGAGACCATA<span class="snp"><tt>N</tt></span>CAGCTCTGCGAGG<span class="snp"><tt>N</tt></span>AGAATGTTAATCCAG 180
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
10539R-3.in silico 121 CACCAAGACACTGAGGGCCAGGAGACCATA<span class="snp"><tt>-</tt></span>CAGCTCTGCGAGG<span class="snp"><tt>-</tt></span>AGAATGTTAATCCAG 180
10539R-3.IR_full 181 GTAAAATCAAGCTGGGACCCAAGGACTTTGAGCTCAAGAAGGTCCTTGGCAAAGGTGGTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 181 GTAAAATCAAGCTGGGACCCAAGGACTTTGAGCTCAAGAAGGTCCTTGGCAAAGGTGGTT 240
10539R-3.IR_full 241 ATGGCAAAGTATTTCAGGTGCGCAAGACCGCTGGACGAGATGCTAACAAATATTTTGCCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 241 ATGGCAAAGTATTTCAGGTGCGCAAGACCGCTGGACGAGATGCTAACAAATATTTTGCCA 300
10539R-3.IR_full 301 TGAAGGTGCTCAAAAAGGCATCCATTGTGACCAATCAAAAGGACACAGCGCACACCCGCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 301 TGAAGGTGCTCAAAAAGGCATCCATTGTGACCAATCAAAAGGACACAGCGCACACCCGCG 360
10539R-3.IR_full 361 CCGAGCGCAATATACTCGAGGCAGTCAAGCATCCCTTCATAGTGGAGCTAGTTTATGCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 361 CCGAGCGCAATATACTCGAGGCAGTCAAGCATCCCTTCATAGTGGAGCTAGTTTATGCCT 420
10539R-3.IR_full 421 TCCAGACAGACGGAAAACTATACCTTATACTTGAATATCTCAGCGGTGGAGAGCTGTTCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10539R-3.in silico 421 TCCAGACAGACGGAAAACTATACCTTATACTTGAATATCTCAGCGGTGGAGAGCTGTTCA 480
10539R-3.IR_full 481 TGCATTTGGAGCGTGAGGGCAT 502
||||||||||||||||||||||
10539R-3.in silico 481 TGCATTTGGAGCGTGAGGGCAT 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
8 |
NM_079217.2 |
CG10539-RA (S6k), mRNA |
| 1.24 |
6 |
3 |
6 |
8 |
NM_078713.2 |
CG17596-RA (S6kII), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_139833.1 |
CG8616-RA (CG8616), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136600.1 |
CG13743-RA (CG13743), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_001043030.1 |
CG17800-RX, transcript variant X (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_001043028.1 |
CG17800-RR, transcript variant R (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043059.1 |
CG17800-PAO (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043038.1 |
CG17800-PAN (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043057.1 |
CG17800-PAM (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043014.1 |
CG17800-PAL (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043045.1 |
CG17800-PAK (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043041.1 |
CG17800-PBE (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043040.1 |
CG17800-PAJ (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043063.1 |
CG17800-PAI (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043013.1 |
CG17800-PBD (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043048.1 |
CG17800-PAH (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043053.1 |
CG17800-PAG (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043062.1 |
CG17800-PAF (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043064.1 |
CG17800-PAE (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043051.1 |
CG17800-PAP (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043023.1 |
CG17800-PAQ (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043027.1 |
CG17800-PAR (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043019.1 |
CG17800-PAS (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_078925.5 |
CG17800-RD, transcript variant D (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043021.1 |
CG17800-PAT (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043037.1 |
CG17800-PAU (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043039.1 |
CG17800-PAV (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043017.1 |
CG17800-PAW (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043015.1 |
CG17800-PAX (Dscam), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043032.1 |
CG17800-PAY (Dscam), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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