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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10532R-1 |
| Symbol |
CG32944 |
Full Name |
CG32944 |
| CG No |
CG32944 |
Old CG No |
CG10532 |
| Synonyms |
CG9818, CG10532, CG32944 |
| Accession No (Link to NCBI) |
NM_176397.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCACTCGAACACCCCACTAGC |
| Primer Seq. 3' |
aatctagaggtaccTCTCTTGGATGGGGGCCAAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCACTCGAAC ACCCCACTAG CGGAGATCAA GAATATATTG AACACCCCCG TGCACTATCC
0061 GCGCTACTGG AGCAGCAACT TCGTCGATCT GCTGCAGAGG TTACTTTCCA CCTATCCGGG
0121 TGCTAGGATC AGTACAAGGC AGGAGCTGCA TCAAACGCCC ATGTTGCGCA ACATTGACTT
0181 CCAGAGGGTG CTCGAGAAGA AGATCAAACC CATCTATAAA CCTCCCGAAG ACCACCTCAA
0241 CTGTGATCCT TGCCTAGAAC TGGAGGAAAT GATTGTAGAA TCGCGGCCCT TGCATAAAAA
0301 GAAGAAGCGC CTGGCCAAGC AGAGGTCAGC ACAACGCGAC AGCGATCCAG AAGGACTGCA
0361 CTGGTCAAAG AGTTCATCGT TTACAACCGC TACAAGGAGT TGAAGCGNAA AGCCATGGAA
0421 CAAAAGGAAA ACGACTGGCA GCGCGAACTG GNAACTGGCC ATGGCCAACT CCATCGTGAA
0481 CAGCTTGGCC CCCATCCAAG AGA |
| in silico PCR Fragment |
0001 GCACTCGAAC ACCCCACTAG CGGAGATCAA GAATATATTG AACACCCCCG TGCACTATCC
0061 GCGCTACTGG AGCAGCAACT TCGTCGATCT GCTGCAGAGG TTACTTTCCA CCTATCCGGG
0121 TGCTAGGATC AGTACAAGGC AGGAGCTGCA TCAAACGCCC ATGTTGCGCA ACATTGACTT
0181 CCAGAGGGTG CTCGAGAAGA AGATCAAACC CATCTATAAA CCTCCCGAAG ACCACCTCAA
0241 CTGTGATCCT TGCCTAGAAC TGGAGGAAAT GATTGTAGAA TCGCGGCCCT TGCATAAAAA
0301 GAAGAAGCGC CTGGCCAAGC AGAGGTCAGC ACAACGCGAC AGCGATCCAG A--GACTGCA
0361 CTGGTCAAAG AGTTCATCGT TTACAACCGC TACAAGGAGT TGAAGCGCAA AGCCATGGAA
0421 CAAAAGGAAA ACGACTGGCA GCGCGAACTG G-AACTGGCC ATGGCCAACT CCATCGTGAA
0481 CAGCTTGGCC CCCATCCAAG AGA |
| Assemble Data |
10532R-1.IR_full 1 GCACTCGAACACCCCACTAGCGGAGATCAAGAATATATTGAACACCCCCGTGCACTATCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-1.in silico 1 GCACTCGAACACCCCACTAGCGGAGATCAAGAATATATTGAACACCCCCGTGCACTATCC 60
10532R-1.IR_full 61 GCGCTACTGGAGCAGCAACTTCGTCGATCTGCTGCAGAGGTTACTTTCCACCTATCCGGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-1.in silico 61 GCGCTACTGGAGCAGCAACTTCGTCGATCTGCTGCAGAGGTTACTTTCCACCTATCCGGG 120
10532R-1.IR_full 121 TGCTAGGATCAGTACAAGGCAGGAGCTGCATCAAACGCCCATGTTGCGCAACATTGACTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-1.in silico 121 TGCTAGGATCAGTACAAGGCAGGAGCTGCATCAAACGCCCATGTTGCGCAACATTGACTT 180
10532R-1.IR_full 181 CCAGAGGGTGCTCGAGAAGAAGATCAAACCCATCTATAAACCTCCCGAAGACCACCTCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-1.in silico 181 CCAGAGGGTGCTCGAGAAGAAGATCAAACCCATCTATAAACCTCCCGAAGACCACCTCAA 240
10532R-1.IR_full 241 CTGTGATCCTTGCCTAGAACTGGAGGAAATGATTGTAGAATCGCGGCCCTTGCATAAAAA 300
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10532R-1.in silico 241 CTGTGATCCTTGCCTAGAACTGGAGGAAATGATTGTAGAATCGCGGCCCTTGCATAAAAA 300
10532R-1.IR_full 301 GAAGAAGCGCCTGGCCAAGCAGAGGTCAGCACAACGCGACAGCGATCCAGAAGGACTGCA 360
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10532R-1.in silico 301 GAAGAAGCGCCTGGCCAAGCAGAGGTCAGCACAACGCGACAGCGATCCAGA--GACTGCA 360
10532R-1.IR_full 361 CTGGTCAAAGAGTTCATCGTTTACAACCGCTACAAGGAGTTGAAGCGNAAAGCCATGGAA 420
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10532R-1.in silico 361 CTGGTCAAAGAGTTCATCGTTTACAACCGCTACAAGGAGTTGAAGCGCAAAGCCATGGAA 420
10532R-1.IR_full 421 CAAAAGGAAAACGACTGGCAGCGCGAACTGGNAACTGGCCATGGCCAACTCCATCGTGAA 480
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
10532R-1.in silico 421 CAAAAGGAAAACGACTGGCAGCGCGAACTGG-AACTGGCCATGGCCAACTCCATCGTGAA 480
10532R-1.IR_full 481 CAGCTTGGCCCCCATCCAAGAGA 503
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10532R-1.in silico 481 CAGCTTGGCCCCCATCCAAGAGA 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
12 |
NM_001043201.1 |
CG32944-RC, transcript variant C (CG32944), mRNA |
| 100 |
482 |
0 |
0 |
12 |
NM_176397.2 |
CG32944-RB, transcript variant B (CG32944), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_079859.2 |
CG1438-RA (Cyp4c3), mRNA |
| 0 |
0 |
7 |
4 |
2 |
NM_132642.2 |
CG15744-RA (CG15744), mRNA |
| 0 |
0 |
4 |
3 |
6 |
NM_132317.1 |
CG12121-RA (CG12121), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_136755.2 |
CG16728-RA (CG16728), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_078740.2 |
CG8817-RB, transcript variant B (lilli), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_164516.1 |
CG8817-RA, transcript variant A (lilli), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_164517.1 |
CG8817-RC, transcript variant C (lilli), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_141291.2 |
CG10018-RA (Snm1), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_141226.2 |
CG1057-RA, transcript variant A (MED31), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169013.1 |
CG1057-RB, transcript variant B (MED31), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142774.2 |
CG18596-RA (CG18596), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
1 |
9 |
4 |
NM_078592.2 |
CG11172-RA (NFAT), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_135824.2 |
CG9014-RA (CG9014), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_078672.3 |
CG7113-RA (scu), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166823.1 |
CG11059-RB, transcript variant B (cals), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079893.2 |
CG11059-RA, transcript variant A (cals), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168470.1 |
CG32085-RA (CG32085), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168781.1 |
CG3961-RA, transcript variant A (CG3961), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140810.2 |
CG3961-RC, transcript variant C (CG3961), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168782.1 |
CG3961-RB, transcript variant B (CG3961), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079823.2 |
CG1395-RA (stg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079992.3 |
CG17976-RA (sut3), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_142954.3 |
CG6057-RA (SMC1), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_137978.1 |
CG18128-RA (CG18128), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_136402.2 |
CG9460-RA (CG9460), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_079059.2 |
CG5519-RA (Gbp), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_132420.2 |
CG32676-RA (CG32676), mRNA |
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