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| NIG-RNAi Strains Detail |
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temporarily unavailable
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10522R-2 |
| Symbol |
sti |
Full Name |
sticky |
| CG No |
CG10522 |
Old CG No |
CG10522 |
| Synonyms |
CG10522, dck, cit, l(3)7m62, l(3)7m-62, l(3)SG21, anon-WO0118547.388, sti |
| Accession No (Link to NCBI) |
NM_140327.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tran THY, Yang DW, Kim M, Lee DH, Gai M, Di Cunto F, Choi KW, Lim DS.
Citron kinase interacts with LATS2 and inhibits its activity by occluding its hydrophobic phosphorylation motif.
J Mol Cell Biol (2019) 11(11) 1006-1017
[
PubMed ID = 30865227
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACCCAAGATGGAGCCGATTA |
| Primer Seq. 3' |
aatctagaggtaccCCATTCAGAGTTGCGAATGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACCCAAGAT GGAGCCGATT ANGCGTGcGN ACCGCGCGTN CTGAACAACC TGATTCTGGG
0061 CAAAGGAGCT GGCGTCTGTG CNGAAGCNCG CTGGAAGCGc CTNCCGGATC AGGTATTCCC
0121 GCCTCCACCA GGAGNAAGCA TTGTACCCGT GAGCACCACT AGCGCCGCCG TgGCCGAGGC
0181 CATCNTGCCG CGAGGGACTC CTGGACGCCT TCTGTCTGCT GTACAACGAG TGCGACAAGG
0241 ATACGCTGAA GANAGCGCGA TCGCAACATC GCCGAGTTTG TCAACAAATT TCGTCCCATC
0301 ATAGAGGAGA CCCGCAAACT CAGGGTCAAC GCAGATGACT TCCTCATCAA GACGCTTATT
0361 GGCCAAGGAT ACTTTGGCAA CGTTCACCTG GTGGTGGAGC GGCAAACCAA CGACATATAC
0421 GCCATGAAGA AGATCAAAAA GTCGGTGGTG ACGACGTCAC AAGTCAAGGA GGAGCGCGAT
0481 ATCATGTCCA TTCGCAACTC TGAATGG |
| in silico PCR Fragment |
0001 CACCCAAGAT GGAGCCGATT A-GCGTGCGC ACCGCGCGT- CTGAACAACC TGATTCTGGG
0061 CAAAGGAGCT GGCGTCTGTG -CGAAGCCCG CTGGAAGCGC CT-CCGGATC AGGTATTCCC
0121 GCCTCCACCA GGAG-AAGCA TTGTACCCGT GAGCACCACT AGCGCCGCCG TGGCCGAGGC
0181 CATC-TGCCG CGAGGGACTC CTGGACGCCT TCTGTCTGCT GTACAACGAG TGCGACAAGG
0241 ATACGCTGAA GA-AGCGCGA TCGCAACATC GCCGAGTTTG TCAACAAATT TCGTCCCATC
0301 ATAGAGGAGA CCCGCAAACT CAGGGTCAAC GCAGATGACT TCCTCATCAA GACGCTTATT
0361 GGCCAAGGAT ACTTTGGCAA CGTTCACCTG GTGGTGGAGC GGCAAACCAA CGACATATAC
0421 GCCATGAAGA AGATCAAAAA GTCGGTGGTG ACGACGTCAC AAGTCAAGGA GGAGCGCGAT
0481 ATCATGTCCA TTCGCAACTC TGAATGG |
| Assemble Data |
10522R-2.IR_full 1 CACCCAAGATGGAGCCGATTANGCGTGCGNACCGCGCGTNCTGAACAACCTGATTCTGGG 60
||||||||||||||||||||| ||||||| ||||||||| ||||||||||||||||||||
10522R-2.in silico 1 CACCCAAGATGGAGCCGATTA-GCGTGCGCACCGCGCGT-CTGAACAACCTGATTCTGGG 60
10522R-2.IR_full 61 CAAAGGAGCTGGCGTCTGTGCNGAAGCNCGCTGGAAGCGCCTNCCGGATCAGGTATTCCC 120
|||||||||||||||||||| ||||| |||||||||||||| |||||||||||||||||
10522R-2.in silico 61 CAAAGGAGCTGGCGTCTGTG-CGAAGCCCGCTGGAAGCGCCT-CCGGATCAGGTATTCCC 120
10522R-2.IR_full 121 GCCTCCACCAGGAGNAAGCATTGTACCCGTGAGCACCACTAGCGCCGCCGTGGCCGAGGC 180
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 121 GCCTCCACCAGGAG-AAGCATTGTACCCGTGAGCACCACTAGCGCCGCCGTGGCCGAGGC 180
10522R-2.IR_full 181 CATCNTGCCGCGAGGGACTCCTGGACGCCTTCTGTCTGCTGTACAACGAGTGCGACAAGG 240
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 181 CATC-TGCCGCGAGGGACTCCTGGACGCCTTCTGTCTGCTGTACAACGAGTGCGACAAGG 240
10522R-2.IR_full 241 ATACGCTGAAGANAGCGCGATCGCAACATCGCCGAGTTTGTCAACAAATTTCGTCCCATC 300
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 241 ATACGCTGAAGA-AGCGCGATCGCAACATCGCCGAGTTTGTCAACAAATTTCGTCCCATC 300
10522R-2.IR_full 301 ATAGAGGAGACCCGCAAACTCAGGGTCAACGCAGATGACTTCCTCATCAAGACGCTTATT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 301 ATAGAGGAGACCCGCAAACTCAGGGTCAACGCAGATGACTTCCTCATCAAGACGCTTATT 360
10522R-2.IR_full 361 GGCCAAGGATACTTTGGCAACGTTCACCTGGTGGTGGAGCGGCAAACCAACGACATATAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 361 GGCCAAGGATACTTTGGCAACGTTCACCTGGTGGTGGAGCGGCAAACCAACGACATATAC 420
10522R-2.IR_full 421 GCCATGAAGAAGATCAAAAAGTCGGTGGTGACGACGTCACAAGTCAAGGAGGAGCGCGAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10522R-2.in silico 421 GCCATGAAGAAGATCAAAAAGTCGGTGGTGACGACGTCACAAGTCAAGGAGGAGCGCGAT 480
10522R-2.IR_full 481 ATCATGTCCATTCGCAACTCTGAATGG 507
|||||||||||||||||||||||||||
10522R-2.in silico 481 ATCATGTCCATTCGCAACTCTGAATGG 507
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_140327.1 |
CG10522-RA (sti), mRNA |
| 0.41 |
2 |
3 |
2 |
3 |
NM_166992.2 |
CG2904-RA (ec), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_133098.2 |
CG7053-RA (CG7053), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_144367.1 |
CG6633-RA (Ugt86Dd), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_001042995.1 |
CG12547-RA (CG12547), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_168387.1 |
CG6711-RA (Taf2), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079325.2 |
CG5433-RA (Klc), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_143722.2 |
CG4913-RA (ear), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_132546.2 |
CG1492-RA (CG1492), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_078664.2 |
CG5870-RA (beta-Spec), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_134584.2 |
CG32512-RA (CG32512), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168452.1 |
CG6199-RB, transcript variant B (CG6199), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_140194.1 |
CG6199-RA, transcript variant A (CG6199), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164453.3 |
CG7254-RA, transcript variant A (GlyP), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001032048.1 |
CG7254-RB, transcript variant B (GlyP), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168360.1 |
CG6767-RB, transcript variant B (CG6767), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140088.2 |
CG6767-RA, transcript variant A (CG6767), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079959.2 |
CG4501-RA (bgm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165018.1 |
CG6167-RB, transcript variant B (PICK1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135738.2 |
CG6167-RA, transcript variant A (PICK1), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_134811.2 |
CG10880-RA (CG10880), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_142590.1 |
CG4465-RA (CG4465), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_136176.2 |
CG13969-RA (bwa), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_138216.1 |
CG13886-RA (CG13886), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_168650.1 |
CG5284-RA, transcript variant A (CG5284), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_140577.1 |
CG5284-RB, transcript variant B (CG5284), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_176446.1 |
CG33208-RD, transcript variant D (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_176448.1 |
CG33208-RG, transcript variant G (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_176449.1 |
CG33208-RH, transcript variant H (MICAL), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_176445.1 |
CG33208-RC, transcript variant C (MICAL), mRNA |
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