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Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10491R-2 
 Symbol vn  Full Name vein 
 CG No CG10491  Old CG No CG10491 
 Synonyms l(3)vn10567, vein, Vein, Vn, CT29452, P1749, ddd, l(3)L6A, wvn, l(3)rF264, l(3)ddd, l(3)10568, l(3)10567, CG10491, vn 
 Accession No (Link to NCBI) NM_079218.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Singh SR, Liu Y, Zhao J, Zeng X, Hou SX.
The novel tumour suppressor Madm regulates stem cell competition in the Drosophila testis.
Nat Commun (2016) 7 10473 [ PubMed ID = 26792023 ] [ RRC reference ]

Xu N, Wang SQ, Tan D, Gao Y, Lin G, Xi R.
EGFR, Wingless and JAK/STAT signaling cooperatively maintain Drosophila intestinal stem cells.
Dev. Biol. (2011) 354(1) 31-43 [ PubMed ID = 21440535 ] [ RRC reference ]

Song W, Kir S, Hong S, Hu Y, Wang X, Binari R, Tang HW, Chung V, Banks AS, Spiegelman B, Perrimon N.
Tumor-Derived Ligands Trigger Tumor Growth and Host Wasting via Differential MEK Activation.
Dev. Cell (2019) [ PubMed ID = 30639055 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTGGGAAGGCAGTGCCGAAGAA 
 Primer Seq. 3'
aatctagaggtaccGACTCGATTAGATAGCTCCGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TGGGAAGGCA GTGcCGNAGA AAGCAGCTAC TATATACCAC TGAGTTCGGA TAATGGCAGC 
0061 GGGAgcNGTG AAAGCTCCgN CGAGAGCGGT AgcNGCAGCA GTAGAAGCAG CAGCAACAAC 
0121 ATCGACAACA ATATCTTAAG TAGGCTGCTC AGCCTCAACA GCAATAGTCT TAGTAGCCGT 
0181 AGCAATGTTA AGTTAAAGCC AGCAACAGTA TTCGACGCCG GCAGCAGTAC ACCCGCTCAG 
0241 CAGGAGCAAC ATGTGGCTGC CGTGCCAGag cAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
0301 TCAATGCAAA AAGTTCCAAA TACGTTAATC AATAGTCAAA TATATAATTT ATTATACAAT 
0361 GGcNNGCCCA GCGAGGCGGC GAGCAGCAAA ATgNGTCGCC ACATTCAACC ATCACAGCTG 
0421 CCACATCAGC CAGAGAgNAG AGCGCAATTA CCGAGCAACT ACAGCAGTCG GNCGGCGGTG 
0481 cGGAGCTATC TAATCGAGTC  
 in silico PCR Fragment
0001 TGGGAAGGCA GTGCCGAAGA AAGCAGCTAC TATATACCAC TGAGTTCGGA TAATGGCAGC 
0061 GGGAGCAGTG AAAGCTCCGC CGAGAGCGGT AGCAGCAGCA GTAGAAGCAG CAGCAACAAC 
0121 ATCGACAACA ATATCTTAAG TAGGCTGCTC AGCCTCAACA GCAATAGTCT TAGTAGCCGT 
0181 AGCAATGTTA AGTTAAAGCC AGCAACAGTA TTCGACGCCG GCAGCAGTAC ACCCGCTCAG 
0241 CAGGAGCAAC ATGTGGCTGC CGTGCCAGAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
0301 TCAATGCAAA AAGTTCCAAA TACGTTAATC AATAGTCAAA TATATAATTT ATTATACAAT 
0361 GGCATGCCCA GCGAGGCGGC GAGCAGCAAA ATGCGTCGCC ACATTCAACC ATCACAGCTG 
0421 CCACATCAGC CAGAGAGCAG AGCGCAATTA CCGAGCAACT ACAGCAGTCG GCCGGCGGTG 
0481 CGGAGCTATC TAATCGAGTC  
 Assemble Data

10491R-2.IR_full       1   TGGGAAGGCAGTGCCGNAGAAAGCAGCTACTATATACCACTGAGTTCGGATAATGGCAGC 60
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
10491R-2.in silico     1   TGGGAAGGCAGTGCCGAAGAAAGCAGCTACTATATACCACTGAGTTCGGATAATGGCAGC 60


10491R-2.IR_full       61  GGGAGCNGTGAAAGCTCCGNCGAGAGCGGTAGCNGCAGCAGTAGAAGCAGCAGCAACAAC 120
                           |||||| |||||||||||| ||||||||||||| ||||||||||||||||||||||||||
10491R-2.in silico     61  GGGAGCAGTGAAAGCTCCGCCGAGAGCGGTAGCAGCAGCAGTAGAAGCAGCAGCAACAAC 120


10491R-2.IR_full       121 ATCGACAACAATATCTTAAGTAGGCTGCTCAGCCTCAACAGCAATAGTCTTAGTAGCCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10491R-2.in silico     121 ATCGACAACAATATCTTAAGTAGGCTGCTCAGCCTCAACAGCAATAGTCTTAGTAGCCGT 180


10491R-2.IR_full       181 AGCAATGTTAAGTTAAAGCCAGCAACAGTATTCGACGCCGGCAGCAGTACACCCGCTCAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10491R-2.in silico     181 AGCAATGTTAAGTTAAAGCCAGCAACAGTATTCGACGCCGGCAGCAGTACACCCGCTCAG 240


10491R-2.IR_full       241 CAGGAGCAACATGTGGCTGCCGTGCCAGAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10491R-2.in silico     241 CAGGAGCAACATGTGGCTGCCGTGCCAGAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAG 300


10491R-2.IR_full       301 TCAATGCAAAAAGTTCCAAATACGTTAATCAATAGTCAAATATATAATTTATTATACAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10491R-2.in silico     301 TCAATGCAAAAAGTTCCAAATACGTTAATCAATAGTCAAATATATAATTTATTATACAAT 360


10491R-2.IR_full       361 GGCNNGCCCAGCGAGGCGGCGAGCAGCAAAATGNGTCGCCACATTCAACCATCACAGCTG 420
                           |||  |||||||||||||||||||||||||||| ||||||||||||||||||||||||||
10491R-2.in silico     361 GGCATGCCCAGCGAGGCGGCGAGCAGCAAAATGCGTCGCCACATTCAACCATCACAGCTG 420


10491R-2.IR_full       421 CCACATCAGCCAGAGAGNAGAGCGCAATTACCGAGCAACTACAGCAGTCGGNCGGCGGTG 480
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||| ||||||||
10491R-2.in silico     421 CCACATCAGCCAGAGAGCAGAGCGCAATTACCGAGCAACTACAGCAGTCGGCCGGCGGTG 480


10491R-2.IR_full       481 CGGAGCTATCTAATCGAGTC 500
                           ||||||||||||||||||||
10491R-2.in silico     481 CGGAGCTATCTAATCGAGTC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.41  484  145  283  573  NM_001014565.1  CG10491-RB, transcript variant B (vn), mRNA 
100.41  484  145  283  573  NM_079218.2  CG10491-RA, transcript variant A (vn), mRNA 
18.46   89  731  1520  2951  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
18.46   89  731  1520  2951  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
18.46   89  731  1520  2951  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
7.05   34  255  641  1365  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
7.05   34  255  641  1365  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
7.05   34  86  472  920  NM_139493.2  CG2083-RA (CG2083), mRNA 
7.05   34  63  482  745  NM_080364.3  CG5461-RA, transcript variant A (bun), mRNA 
7.05   34  59  457  686  NM_001042894.1  CG5461-RF, transcript variant F (bun), mRNA 
6.84   33  54  266  513  NM_132004.2  CG4136-RA (CG4136), mRNA 
6.63   32  90  355  586  NM_167000.1  CG32778-RA (CG32778), mRNA 
6.22   30  140  450  908  NM_079903.2  CG15319-RB (nej), mRNA 
6.01   29  167  427  951  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
6.01   29  74  353  432  NM_206456.1  CG32466-RB, transcript variant B (rn), mRNA 
5.8   28  42  157  290  NM_057511.3  CG3936-RA (N), mRNA 
5.6   27  161  428  929  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
5.6   27  161  428  929  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
5.6   27  80  218  395  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
5.6   27  80  218  395  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
5.39   26  58  260  574  NM_079996.2  CG18024-RA (SoxN), mRNA 
5.39   26  57  276  366  NM_143700.3  CG17724-RA, transcript variant A (CG17724), mRNA 
5.18   25  93  214  574  NM_169386.1  CG17228-RA, transcript variant A (pros), mRNA 
5.18   25  76  267  565  NM_079507.2  CG2530-RA (corto), mRNA 
4.97   24  166  604  1170  NM_001038734.1  CG16902-RC (Hr4), mRNA 
4.97   24  103  320  600  NM_143575.2  CG12071-RA, transcript variant A (CG12071), mRNA 
4.97   24  103  320  600  NM_176592.1  CG12071-RB, transcript variant B (CG12071), mRNA 
4.97   24  18  166  229  NM_140438.2  CG32139-RA (Sox21b), mRNA 
4.56   22  147  368  794  NM_169786.1  CG7847-RB, transcript variant B (sr), mRNA 
4.56   22  138  420  630  NM_134474.4  CG32532-RA (CG32532), mRNA 

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