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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10444R-2 
 Symbol CG10444  Full Name CG10444 
 CG No CG10444  Old CG No CG10444 
 Synonyms CG10444 
 Accession No (Link to NCBI) NM_137621.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, D├╝rrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCAACATTGCCTACGTGCTGAG 
 Primer Seq. 3'
aatctagaggtaccAGAGGTAAGTGACCATGCCGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CAACATTGCC TACGTGCTGA GTACGCCCAT TGCACGCGTA CTTCTTCCTT CCTGTTTTCT 
0061 ACAGGATGCG GACGACCAGT GTGTACGAGT ATCTGGAGCG GCGCTTTGGC CAGGCCACCC 
0121 GGCTGTCCGC CTCCCTGGCC TTCACCGTGC AAATGGTGCT TTACATGGGC ATTGCACTCT 
0181 ATGCACCAGC GCTCGCTTTA GAAGCTGTAA CCGGCATTCA TCGGTCAATG GCCATCGTGG 
0241 TCATTGGACT GGTGTGCACC TTCTATTCGA CACTTGGCGG CCTGAANGGC GGTGCTCATC 
0301 ACGGATGTGT TTCAGTCCTT CCTCATGTTT GCCGCCATCT ACGCCGTGAT TGCCGTGTCG 
0361 GCCATTAAAG CCGGCGGATT CGCGGCCATC TGGGATGTGG CTGTGGAGCG AGGACGCGTG 
0421 AACTTCATTG AGTTTTCATT GGATCCCACC GTGAGGCATA CTTGGTGGTC TCTGATCATT 
0481 GGCGGCATGG TCACTTAcCT CT 
 in silico PCR Fragment
0001 CAACATTGCC TACGTGCTGA GTACGCCCAT TGC-CGCGTA CTTCTTCCTT CCTGTTTTCT 
0061 ACAGGATGCG GACGACCAGT GTGTACGAGT ATCTGGAGCG GCGCTTTGGC CAGGCCACCC 
0121 GGCTGTCCGC CTCCCTGGCC TTCACCGTGC AAATGGTGCT TTACATGGGC ATTGCACTCT 
0181 ATGCACCAGC GCTCGCTTTA GAAGCTGTAA CCGGCATTCA TCGGTCAATG GCCATCGTGG 
0241 TCATTGGACT GGTGTGCACC TTCTATTCGA CACTTGGCGG CCTGAA-GGC GGTGCTCATC 
0301 ACGGATGTGT TTCAGTCCTT CCTCATGTTT GCCGCCATCT ACGCCGTGAT TGCCGTGTCG 
0361 GCCATTAAAG CCGGCGGATT CGCGGCCATC TGGGATGTGG CTGTGGAGCG AGGACGCGTG 
0421 AACTTCATTG AGTTTTCATT GGATCCCACC GTGAGGCATA CTTGGTGGTC TCTGATCATT 
0481 GGCGGCATGG TCACTTACCT CT 
 Assemble Data

10444R-2.IR_full       1   CAACATTGCCTACGTGCTGAGTACGCCCATTGCACGCGTACTTCTTCCTTCCTGTTTTCT 60
                           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
10444R-2.in silico     1   CAACATTGCCTACGTGCTGAGTACGCCCATTGC-CGCGTACTTCTTCCTTCCTGTTTTCT 60


10444R-2.IR_full       61  ACAGGATGCGGACGACCAGTGTGTACGAGTATCTGGAGCGGCGCTTTGGCCAGGCCACCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     61  ACAGGATGCGGACGACCAGTGTGTACGAGTATCTGGAGCGGCGCTTTGGCCAGGCCACCC 120


10444R-2.IR_full       121 GGCTGTCCGCCTCCCTGGCCTTCACCGTGCAAATGGTGCTTTACATGGGCATTGCACTCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     121 GGCTGTCCGCCTCCCTGGCCTTCACCGTGCAAATGGTGCTTTACATGGGCATTGCACTCT 180


10444R-2.IR_full       181 ATGCACCAGCGCTCGCTTTAGAAGCTGTAACCGGCATTCATCGGTCAATGGCCATCGTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     181 ATGCACCAGCGCTCGCTTTAGAAGCTGTAACCGGCATTCATCGGTCAATGGCCATCGTGG 240


10444R-2.IR_full       241 TCATTGGACTGGTGTGCACCTTCTATTCGACACTTGGCGGCCTGAANGGCGGTGCTCATC 300
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
10444R-2.in silico     241 TCATTGGACTGGTGTGCACCTTCTATTCGACACTTGGCGGCCTGAA-GGCGGTGCTCATC 300


10444R-2.IR_full       301 ACGGATGTGTTTCAGTCCTTCCTCATGTTTGCCGCCATCTACGCCGTGATTGCCGTGTCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     301 ACGGATGTGTTTCAGTCCTTCCTCATGTTTGCCGCCATCTACGCCGTGATTGCCGTGTCG 360


10444R-2.IR_full       361 GCCATTAAAGCCGGCGGATTCGCGGCCATCTGGGATGTGGCTGTGGAGCGAGGACGCGTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     361 GCCATTAAAGCCGGCGGATTCGCGGCCATCTGGGATGTGGCTGTGGAGCGAGGACGCGTG 420


10444R-2.IR_full       421 AACTTCATTGAGTTTTCATTGGATCCCACCGTGAGGCATACTTGGTGGTCTCTGATCATT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10444R-2.in silico     421 AACTTCATTGAGTTTTCATTGGATCCCACCGTGAGGCATACTTGGTGGTCTCTGATCATT 480


10444R-2.IR_full       481 GGCGGCATGGTCACTTACCTCT 502
                           ||||||||||||||||||||||
10444R-2.in silico     481 GGCGGCATGGTCACTTACCTCT 502

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_137621.2  CG10444-RA (CG10444), mRNA 
0.82   15  47  NM_167256.1  CG32669-RA (CG32669), mRNA 
0   NM_135550.1  CG5362-RA (CG5362), mRNA 
0   NM_057369.3  CG12181-RB (Sgs4), mRNA 
0   NM_136574.2  CG8258-RA (CG8258), mRNA 
0   NM_079629.2  CG7390-RA, transcript variant A (smp-30), mRNA 
0   NM_169602.1  CG7390-RB, transcript variant B (smp-30), mRNA 
0   NM_057813.3  CG6141-RA, transcript variant A (RpL9), mRNA 
0   NM_164955.1  CG6141-RB, transcript variant B (RpL9), mRNA 
0   NM_166616.1  CG5411-RA, transcript variant A (Pde8), mRNA 
0   NM_137970.3  CG5411-RE, transcript variant E (Pde8), mRNA 
0   NM_166617.1  CG5411-RD, transcript variant D (Pde8), mRNA 
0   NM_166618.1  CG5411-RB, transcript variant B (Pde8), mRNA 
0   NM_001014729.1  CG32697-RE, transcript variant E (l(1)G0232), mRNA 
0   NM_167199.1  CG32697-RD, transcript variant D (l(1)G0232), mRNA 
0   NM_132348.2  CG32697-RA, transcript variant A (l(1)G0232), mRNA 
0   NM_001014728.1  CG32697-RF, transcript variant F (l(1)G0232), mRNA 
0   NM_167197.1  CG32697-RC, transcript variant C (l(1)G0232), mRNA 
0   NM_167198.1  CG32697-RB, transcript variant B (l(1)G0232), mRNA 
0   NM_135848.2  CG16873-RA (CG16873), mRNA 
0   NM_137986.2  CG13561-RA (CG13561), mRNA 
0   NM_140250.1  CG5964-RA (CG5964), mRNA 
0   NM_144358.2  CG14233-RA (meso18E), mRNA 
0   NM_143460.2  CG7601-RA (CG7601), mRNA 
0   NM_167408.1  CG32600-RA (dpr8), mRNA 
0   10  15  NM_165304.1  CG10084-RB, transcript variant B (swm), mRNA 
0   10  NM_136132.4  CG10084-RA, transcript variant A (swm), mRNA 
0   10  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   NM_078873.2  CG15162-RA (MESR3), mRNA 
0   NM_170116.1  CG5422-RB, transcript variant B (Rox8), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
+- 
DC
++ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
++ 
DM
VM
 
VB
 
Dcompartment   Penetrance
     

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