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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10435R-3

Symbol CG10435 Full Name CG10435

CG No CG10435 Old CG No CG10435

Synonyms CG10435

Accession No (Link to NCBI) NM_141502.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCACCAGACACAGCTGGAGAA

Primer Seq. 3'

aatctagaggtaccTGTCAGGCACACTCCTTGACT

Predicted Fragment Size

IR fragment full Seq

0001 GCACCAGACA CAGCTGGAGA AGCGCATAGA TCAACAGCAG CAGGATCAGC AGCACCAGGA 
0061 GCCGCCGCAT GAGGATCATT CCAAGGAGGA GCAATCTGGC AATGAANGTG CCAGCCTCCG 
0121 GTGGAGCTAN GCAATGACTA CGCTGGCGGA GACCGAAAGC GACGCTCGAC AGCGTCAATT 
0181 TCCATGTTCA CTGTGATGAT AATGCGGACA GGGGCGAGCC AAGCCACATT CCAACCACGG 
0241 CTCAATACCT AACGCCCATG TANCAGCGCT TCCGCCCAGC AGCCGTTGCA TAAACCGGAA 
0301 CACTTTCAGG TGTACCACAT GCAGGGCTAC AGTCACCAGC CCTTCTACCC TCCGCAGCAG 
0361 CATTCTTTCC AACAGTTTAG CAACTACCAA GTCAATGGAT TCGGGACTCA AAACCTTTTG 
0421 AAGGCTCCTC CAGCGCCTTC CTCCGTGCAT GCGCTCAGCC AGTCGGAGGA CGAGGTAGCC 
0481 ACGAAGTCAA GGAGTGTGCC TGACA

in silico PCR Fragment

0001 GCACCAGACA CAGCTGGAGA AGCGCATAGA TCAACAGCAG CAGGATCAGC AGCAGCAGGA 
0061 GCCGCCGCAT GAGGATCATT CCAAGGAGGA GCAATCTGGC AATGAA-GTG CCAG-CTCCG 
0121 GTGGAGCTA- GCAATGACTA CGCTGGCGGA GACC-AAAGC GACGCTCGAC AGCGTCAATT 
0181 TCCATGTTCA CTGTGATGAT AATGCGGACA GGGGCGAGCC AAGCCACATT CCAACCACGG 
0241 CTCAATACCT AACGCCCATG TA-CAGCGCT TCCGCCCAGC AGCCGTTGCA TAAACCGGAA 
0301 CACTTTCAGG TGTACCACAT GCAGGGCTAC AGTCACCAGC CCTTCTACCC TCCGCAGCAG 
0361 CATTCTTTCC AACAGTTTAG CAACTACCAA GTCAATGGAT TCGGGACTCA AAACCTTTTG 
0421 AAGGCTCCTC CAGCGCCTTC CTCCGTGCAT GCGCTCAGCC AGTCGGAGGA CGAGGTAGCC 
0481 ACGAAGTCAA GGAGTGTGCC TGACA

Assemble Data


10435R-3.IR_full       1   GCACCAGACACAGCTGGAGAAGCGCATAGATCAACAGCAGCAGGATCAGCAGCACCAGGA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
10435R-3.in silico     1   GCACCAGACACAGCTGGAGAAGCGCATAGATCAACAGCAGCAGGATCAGCAGCAGCAGGA 60


10435R-3.IR_full       61  GCCGCCGCATGAGGATCATTCCAAGGAGGAGCAATCTGGCAATGAANGTGCCAGCCTCCG 120
                           |||||||||||||||||||||||||||||||||||||||||||||| ||||||| |||||
10435R-3.in silico     61  GCCGCCGCATGAGGATCATTCCAAGGAGGAGCAATCTGGCAATGAA-GTGCCAG-CTCCG 120


10435R-3.IR_full       121 GTGGAGCTANGCAATGACTACGCTGGCGGAGACCGAAAGCGACGCTCGACAGCGTCAATT 180
                           ||||||||| |||||||||||||||||||||||| |||||||||||||||||||||||||
10435R-3.in silico     121 GTGGAGCTA-GCAATGACTACGCTGGCGGAGACC-AAAGCGACGCTCGACAGCGTCAATT 180


10435R-3.IR_full       181 TCCATGTTCACTGTGATGATAATGCGGACAGGGGCGAGCCAAGCCACATTCCAACCACGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10435R-3.in silico     181 TCCATGTTCACTGTGATGATAATGCGGACAGGGGCGAGCCAAGCCACATTCCAACCACGG 240


10435R-3.IR_full       241 CTCAATACCTAACGCCCATGTANCAGCGCTTCCGCCCAGCAGCCGTTGCATAAACCGGAA 300
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
10435R-3.in silico     241 CTCAATACCTAACGCCCATGTA-CAGCGCTTCCGCCCAGCAGCCGTTGCATAAACCGGAA 300


10435R-3.IR_full       301 CACTTTCAGGTGTACCACATGCAGGGCTACAGTCACCAGCCCTTCTACCCTCCGCAGCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10435R-3.in silico     301 CACTTTCAGGTGTACCACATGCAGGGCTACAGTCACCAGCCCTTCTACCCTCCGCAGCAG 360


10435R-3.IR_full       361 CATTCTTTCCAACAGTTTAGCAACTACCAAGTCAATGGATTCGGGACTCAAAACCTTTTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10435R-3.in silico     361 CATTCTTTCCAACAGTTTAGCAACTACCAAGTCAATGGATTCGGGACTCAAAACCTTTTG 420


10435R-3.IR_full       421 AAGGCTCCTCCAGCGCCTTCCTCCGTGCATGCGCTCAGCCAGTCGGAGGACGAGGTAGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10435R-3.in silico     421 AAGGCTCCTCCAGCGCCTTCCTCCGTGCATGCGCTCAGCCAGTCGGAGGACGAGGTAGCC 480


10435R-3.IR_full       481 ACGAAGTCAAGGAGTGTGCCTGACA 505
                           |||||||||||||||||||||||||
10435R-3.in silico     481 ACGAAGTCAAGGAGTGTGCCTGACA 505

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	16	NM_141502.2	CG10435-RA (CG10435), mRNA
0.62	3	3	6	12	NM_168935.1	CG32447-RA, transcript variant A (CG32447), mRNA
0.62	3	3	6	12	NM_206422.1	CG32447-RB, transcript variant B (CG32447), mRNA
0.62	3	2	4	4	NM_141323.1	CG2097-RA (CG2097), mRNA
0.41	2	4	5	5	NM_142075.1	CG14358-RA (CG14358), mRNA
0.2	1	10	15	9	NM_142007.2	CG8483-RA (CG8483), mRNA
0.2	1	4	7	52	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	14	29	65	NM_169177.1	CG2507-RB, transcript variant B (sas), mRNA
0	0	13	18	71	NM_164960.1	CG32830-RA (CG32830), mRNA
0	0	12	11	26	NM_136028.2	CG7180-RA (CG7180), mRNA
0	0	11	64	206	NM_132665.1	CG15753-RA (CG15753), mRNA
0	0	9	38	182	NM_078575.2	CG9355-RA (dy), mRNA
0	0	9	20	82	NM_079318.2	CG10488-RA, transcript variant A (eyg), mRNA
0	0	9	20	82	NM_001014582.1	CG10488-RB, transcript variant B (eyg), mRNA
0	0	9	11	65	NM_130728.1	CG15240-RA (CG15240), mRNA
0	0	8	44	218	NM_206089.1	CG33473-RB (luna), mRNA
0	0	8	42	173	NM_078797.2	CG13109-RA (tai), mRNA
0	0	8	31	222	NM_169696.1	CG3992-RA, transcript variant A (srp), mRNA
0	0	8	31	222	NM_169694.1	CG3992-RB, transcript variant B (srp), mRNA
0	0	8	17	129	NM_001032019.1	CG3992-RD, transcript variant D (srp), mRNA
0	0	8	8	51	NM_167279.1	CG18361-RB, transcript variant B (dsh), mRNA
0	0	8	8	51	NM_078563.2	CG18361-RA, transcript variant A (dsh), mRNA
0	0	7	99	417	NM_001038734.1	CG16902-RC (Hr4), mRNA
0	0	7	32	145	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	7	13	60	NM_001043260.1	CG34157-RD, transcript variant D (Dys), mRNA
0	0	7	4	48	NM_136600.1	CG13743-RA (CG13743), mRNA
0	0	7	3	5	NM_135940.2	CG17329-RA (CG17329), mRNA
0	0	6	103	462	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	6	103	462	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	6	43	162	NM_165217.1	CG6667-RA, transcript variant A (dl), mRNA

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