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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10315R-1 |
| Symbol |
eIF2B-delta |
Full Name |
eIF2B-delta |
| CG No |
CG10315 |
Old CG No |
CG10315 |
| Synonyms |
CG10315, eIF2Bdelta, eIF2B-delta |
| Accession No (Link to NCBI) |
NM_137946.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCGAACAGGTGCCACAGACT |
| Primer Seq. 3' |
aatctagaggtaccGCTTTCTTGGCTGCCTTCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCGAACAGG TGCCACAGAC TTTGAAGAGC AAGCACAAGA AGCAAAGGCA ACGCAATCGA
0061 CCGCGCAACA AACGAAATCG CCTGGAAGAC AGCCATTCAA CGACACAAGA AACATCAGCC
0121 ATCGACGCCA CGAGTGCAAT GCCCGGAAAT GCTGTGGATC CATTGGCCAC GGAGAAGACT
0181 CGTGACCAGA TAATGGCCGA GCGGGAGGCG AAGAAGCTGG CTAAGCAGGC CAAGAAACAG
0241 AAAACTGCAC CGACTCCTAC TGCCGCCACT CCTGCTGTTC TGACCACTCC CTCTGCTCCG
0301 GCCACCAAAA TCATCACTGA AGTGGAGCAG ACCACCATTA CCACTAAGCT GACGACCACC
0361 ACCACAACAA AGGTGCAGGA TGCAGTGGCT TCAAAGACGA CTCAGCCAGC TGCGGTAAAT
0421 GGCAAGGATG CGGAGGCGGG TGAAAAGACA CGCGAGCAGA TCAAgGNGGA ACgCGAGGCC
0481 AAGAAGGCAG CCAAGAAAGC |
| in silico PCR Fragment |
0001 AGCGAACAGG TGCCACAGAC TTTGAAGAGC AAGCACAAGA AGCAAAGGCA ACGCAATCGA
0061 CCGCGCAACA AACGAAATCG CCTGGAAGAC AGCCATTCAA CGACACAAGA AACATCAGCC
0121 ATCGACGCCA CGAGTGCAAT GCCCGGAAAT GCTGTGGATC CATTGGCCAC GGAGAAGACT
0181 CGTGACCAGA TAATGGCCGA GCGGGAGGCG AAGAAGCTGG CTAAGCAGGC CAAGAAACAG
0241 AAAACTGCAC CGACTCCTAC TGCCGCCACT CCTGCTGTTC TGACCACTCC CTCTGCTCCG
0301 GCCACCAAAA TCATCACTGA AGTGGAGCAG ACCACCATTA CCACTAAGCT GACGACCACC
0361 ACCACAACAA AGGTGCAGGA TGCAGTGGCT TCAAAGACGA CTCAGCCAGC TGCGGTAAAT
0421 GGCAAGGATG CGGAGGCGGG TGAAAAGACA CGCGAGCAGA TCAAGGCGGA ACGCGAGGCC
0481 AAGAAGGCAG CCAAGAAAGC |
| Assemble Data |
10315R-1.IR_full 1 AGCGAACAGGTGCCACAGACTTTGAAGAGCAAGCACAAGAAGCAAAGGCAACGCAATCGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 1 AGCGAACAGGTGCCACAGACTTTGAAGAGCAAGCACAAGAAGCAAAGGCAACGCAATCGA 60
10315R-1.IR_full 61 CCGCGCAACAAACGAAATCGCCTGGAAGACAGCCATTCAACGACACAAGAAACATCAGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 61 CCGCGCAACAAACGAAATCGCCTGGAAGACAGCCATTCAACGACACAAGAAACATCAGCC 120
10315R-1.IR_full 121 ATCGACGCCACGAGTGCAATGCCCGGAAATGCTGTGGATCCATTGGCCACGGAGAAGACT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 121 ATCGACGCCACGAGTGCAATGCCCGGAAATGCTGTGGATCCATTGGCCACGGAGAAGACT 180
10315R-1.IR_full 181 CGTGACCAGATAATGGCCGAGCGGGAGGCGAAGAAGCTGGCTAAGCAGGCCAAGAAACAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 181 CGTGACCAGATAATGGCCGAGCGGGAGGCGAAGAAGCTGGCTAAGCAGGCCAAGAAACAG 240
10315R-1.IR_full 241 AAAACTGCACCGACTCCTACTGCCGCCACTCCTGCTGTTCTGACCACTCCCTCTGCTCCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 241 AAAACTGCACCGACTCCTACTGCCGCCACTCCTGCTGTTCTGACCACTCCCTCTGCTCCG 300
10315R-1.IR_full 301 GCCACCAAAATCATCACTGAAGTGGAGCAGACCACCATTACCACTAAGCTGACGACCACC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 301 GCCACCAAAATCATCACTGAAGTGGAGCAGACCACCATTACCACTAAGCTGACGACCACC 360
10315R-1.IR_full 361 ACCACAACAAAGGTGCAGGATGCAGTGGCTTCAAAGACGACTCAGCCAGCTGCGGTAAAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10315R-1.in silico 361 ACCACAACAAAGGTGCAGGATGCAGTGGCTTCAAAGACGACTCAGCCAGCTGCGGTAAAT 420
10315R-1.IR_full 421 GGCAAGGATGCGGAGGCGGGTGAAAAGACACGCGAGCAGATCAAGGNGGAACGCGAGGCC 480
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
10315R-1.in silico 421 GGCAAGGATGCGGAGGCGGGTGAAAAGACACGCGAGCAGATCAAGGCGGAACGCGAGGCC 480
10315R-1.IR_full 481 AAGAAGGCAGCCAAGAAAGC 500
||||||||||||||||||||
10315R-1.in silico 481 AAGAAGGCAGCCAAGAAAGC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_137946.2 |
CG10315-RA, transcript variant A (eIF2B-delta), mRNA |
| 96.88 |
467 |
2 |
1 |
3 |
NM_166601.1 |
CG10315-RB, transcript variant B (eIF2B-delta), mRNA |
| 96.68 |
466 |
1 |
1 |
4 |
NM_166602.1 |
CG10315-RC, transcript variant C (eIF2B-delta), mRNA |
| 0 |
0 |
4 |
1 |
6 |
NM_141993.2 |
CG7518-RB, transcript variant B (CG7518), mRNA |
| 0 |
0 |
4 |
1 |
6 |
NM_169489.1 |
CG7518-RA, transcript variant A (CG7518), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_140499.2 |
CG6876-RA (CG6876), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_057782.3 |
CG3954-RA, transcript variant A (csw), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_057783.2 |
CG3954-RB, transcript variant B (csw), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_166928.1 |
CG3954-RC, transcript variant C (csw), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_136171.2 |
CG16772-RA (CG16772), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142963.2 |
CG6173-RA (kal-1), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143465.1 |
CG1973-RA (CG1973), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_167231.3 |
CG17255-RB, transcript variant B (CG17255), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_132403.3 |
CG17255-RA, transcript variant A (CG17255), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_142787.1 |
CG12499-RA (CG12499), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_168947.1 |
CG7139-RB, transcript variant B (CG7139), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_141121.2 |
CG7139-RA, transcript variant A (CG7139), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_001014630.1 |
CG33547-RA (Rim), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_136593.1 |
CG8181-RA (CG8181), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_164832.1 |
CG9280-RC, transcript variant C (Glt), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_165257.1 |
CG31790-RA (CG31790), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_058156.2 |
CG9280-RA, transcript variant A (Glt), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_164831.1 |
CG9280-RB, transcript variant B (Glt), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_058114.3 |
CG14902-RA (decay), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079202.2 |
CG7452-RA (Syx17), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079792.2 |
CG5441-RA (dei), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168084.1 |
CG11347-RD, transcript variant D (CG11347), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139643.1 |
CG11347-RA, transcript variant A (CG11347), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168083.1 |
CG11347-RB, transcript variant B (CG11347), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168085.1 |
CG11347-RC, transcript variant C (CG11347), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
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Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
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