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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10279R-1 |
| Symbol |
Rm62 |
Full Name |
Rm62 |
| CG No |
CG10279 |
Old CG No |
CG10279 |
| Synonyms |
CG10279, Dmp68, p68, Lip, RM62, DmRH8, 4136, l(3)s5196, l(3)rG338, l(3)j3D5, l(3)j3D2, l(3)01086, Rm62 |
| Accession No (Link to NCBI) |
NM_079519.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Liaw GJ, Chiang CS.<br> Inactive Tlk associating with Tak1 increases p38 MAPK activity to prolong the G2 phase.<br> Sci Rep (2019) 9(1) 1885
[
PubMed ID = 30760733
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGGTGGTGGTGGATTCGGGGA |
| Primer Seq. 3' |
aatctagaggtaccATTCGGTGGCCACCTGCTGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGGTGGTGGT GGATTCGGGG ATCGCCGAGG AGGAGGTGGC GGCGGCAGCC AAGACCTCCC
0061 CATGCGTCCG GTCGACTTCT CCAACCTGGC TCCCTTCAAG AAGAACTTTT ACCAGGAGCA
0121 CCCTAACGTA GCAAACCGAT CGCCCTACGA AGTTCAGAGG TATCGCGAAG AGCAGGAGAT
0181 CACCGTGCGC GGACAGGTGC CGAACCCCAT CCAGGACTTC TCCGAGGTCC ATCTGCCCGA
0241 CTACGTCATG AAGGAGATNC CGCCGACANG GCTNACAAGG CCCCCACCGC TATCCAAGCG
0301 CAGGGCTGGC CTATCGCCAT GAGTGGCTCG AACTTCGTCG GCATTGCCAA GACGGGATCC
0361 GGAAAGACCC TGGGCTACAT CCTGCCCGCT ATTGTCCACA TCAACAACCA GCAGCCGCTG
0421 CAGAGGGGCG ACGGACCTAT CGCCCTCGTG CTGGCCCCCA CTCTGGGGAG CTGGCCCAAC
0481 AGATCCAGCA GGTGGCCACC GAAT |
| in silico PCR Fragment |
0001 AGGTGGTGGT GGATTCGGGG ATCGCCGAGG AGGAGGTGGC GGCGGCAGCC AAGACCTCCC
0061 CATGCGTCCG GTCGACTTCT CCAACCTGGC TCCCTTCAAG AAGAACTTTT ACCAGGAGCA
0121 CCCTAACGTA GCAAACCGAT CGCCCTACGA AGTTCAGAGG TATCGCGAAG AGCAGGAGAT
0181 CACCGTGCGC GGACAGGTGC CGAACCCCAT CCAGGACTTC TCCGAGGTCC ATCTGCCCGA
0241 CTACGTCATG AAGGAGAT-C CGCCGACAGG GCT-ACAAGG CCCCCACCGC TATCCAAGCG
0301 CAGGGCTGGC CTATCGCCAT GAGTGGCTCG AACTTCGTCG GCATTGCCAA GACGGGATCC
0361 GGAAAGACCC TGGGCTACAT CCTGCCCGCT ATTGTCCACA TCAACAACCA GCAGCCGCTG
0421 CAGAGGGGCG ACGGACCTAT CGCCCTCGTG CTGGCCCCCA CTC--GGGAG CTGGCCCAAC
0481 AGATCCAGCA GGTGGCCACC GAAT |
| Assemble Data |
<PRE>
10279R-1.IR_full 1 AGGTGGTGGTGGATTCGGGGATCGCCGAGGAGGAGGTGGCGGCGGCAGCCAAGACCTCCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 1 AGGTGGTGGTGGATTCGGGGATCGCCGAGGAGGAGGTGGCGGCGGCAGCCAAGACCTCCC 60
10279R-1.IR_full 61 CATGCGTCCGGTCGACTTCTCCAACCTGGCTCCCTTCAAGAAGAACTTTTACCAGGAGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 61 CATGCGTCCGGTCGACTTCTCCAACCTGGCTCCCTTCAAGAAGAACTTTTACCAGGAGCA 120
10279R-1.IR_full 121 CCCTAACGTAGCAAACCGATCGCCCTACGAAGTTCAGAGGTATCGCGAAGAGCAGGAGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 121 CCCTAACGTAGCAAACCGATCGCCCTACGAAGTTCAGAGGTATCGCGAAGAGCAGGAGAT 180
10279R-1.IR_full 181 CACCGTGCGCGGACAGGTGCCGAACCCCATCCAGGACTTCTCCGAGGTCCATCTGCCCGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 181 CACCGTGCGCGGACAGGTGCCGAACCCCATCCAGGACTTCTCCGAGGTCCATCTGCCCGA 240
10279R-1.IR_full 241 CTACGTCATGAAGGAGAT<span class="snp"><tt>N</tt></span>CCGCCGACA<span class="snp"><tt>N</tt></span>GGCT<span class="snp"><tt>N</tt></span>ACAAGGCCCCCACCGCTATCCAAGCG 300
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||
10279R-1.in silico 241 CTACGTCATGAAGGAGAT<span class="snp"><tt>-</tt></span>CCGCCGACA<span class="snp"><tt>G</tt></span>GGCT<span class="snp"><tt>-</tt></span>ACAAGGCCCCCACCGCTATCCAAGCG 300
10279R-1.IR_full 301 CAGGGCTGGCCTATCGCCATGAGTGGCTCGAACTTCGTCGGCATTGCCAAGACGGGATCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 301 CAGGGCTGGCCTATCGCCATGAGTGGCTCGAACTTCGTCGGCATTGCCAAGACGGGATCC 360
10279R-1.IR_full 361 GGAAAGACCCTGGGCTACATCCTGCCCGCTATTGTCCACATCAACAACCAGCAGCCGCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10279R-1.in silico 361 GGAAAGACCCTGGGCTACATCCTGCCCGCTATTGTCCACATCAACAACCAGCAGCCGCTG 420
10279R-1.IR_full 421 CAGAGGGGCGACGGACCTATCGCCCTCGTGCTGGCCCCCACTC<span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span>GGGAGCTGGCCCAAC 480
|||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>|||||||||||||||
10279R-1.in silico 421 CAGAGGGGCGACGGACCTATCGCCCTCGTGCTGGCCCCCACTC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GGGAGCTGGCCCAAC 480
10279R-1.IR_full 481 AGATCCAGCAGGTGGCCACCGAAT 504
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10279R-1.in silico 481 AGATCCAGCAGGTGGCCACCGAAT 504
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
9 |
33 |
NM_169119.1 |
CG10279-RE, transcript variant E (Rm62), mRNA |
| 100 |
482 |
0 |
9 |
33 |
NM_169118.1 |
CG10279-RD, transcript variant D (Rm62), mRNA |
| 100 |
482 |
0 |
9 |
33 |
NM_169120.1 |
CG10279-RC, transcript variant C (Rm62), mRNA |
| 100 |
482 |
0 |
9 |
33 |
NM_079519.2 |
CG10279-RA, transcript variant A (Rm62), mRNA |
| 100 |
482 |
0 |
9 |
33 |
NM_169122.1 |
CG10279-RB, transcript variant B (Rm62), mRNA |
| 100 |
482 |
0 |
9 |
33 |
NM_169121.1 |
CG10279-RF, transcript variant F (Rm62), mRNA |
| 0.82 |
4 |
2 |
2 |
8 |
NM_141334.1 |
CG10979-RA (CG10979), mRNA |
| 0.2 |
1 |
4 |
2 |
8 |
NM_143622.2 |
CG1800-RA (pasha), mRNA |
| 0 |
0 |
12 |
19 |
19 |
NM_132196.2 |
CG10777-RB (CG10777), mRNA |
| 0 |
0 |
7 |
11 |
52 |
NM_057695.3 |
CG4038-RA (CG4038), mRNA |
| 0 |
0 |
5 |
20 |
64 |
NM_078641.2 |
CG3606-RB, transcript variant B (caz), mRNA |
| 0 |
0 |
5 |
20 |
64 |
NM_167493.1 |
CG3606-RA, transcript variant A (caz), mRNA |
| 0 |
0 |
5 |
13 |
51 |
NM_139805.1 |
CG10077-RA, transcript variant A (CG10077), mRNA |
| 0 |
0 |
5 |
5 |
16 |
NM_168379.1 |
CG32050-RA (CG32050), mRNA |
| 0 |
0 |
4 |
14 |
26 |
NM_167620.2 |
CG32547-RA (CG32547), mRNA |
| 0 |
0 |
4 |
10 |
15 |
NM_079824.2 |
CG18741-RB, transcript variant B (DopR2), mRNA |
| 0 |
0 |
4 |
10 |
15 |
NM_170420.1 |
CG18741-RA, transcript variant A (DopR2), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_135952.1 |
CG5953-RA, transcript variant A (CG5953), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_165165.1 |
CG5953-RB, transcript variant B (CG5953), mRNA |
| 0 |
0 |
3 |
10 |
12 |
NM_137995.2 |
CG18426-RA (ytr), mRNA |
| 0 |
0 |
3 |
9 |
25 |
NM_165965.1 |
CG3884-RA, transcript variant A (CG3884), mRNA |
| 0 |
0 |
3 |
9 |
18 |
NM_142622.1 |
CG4360-RA (CG4360), mRNA |
| 0 |
0 |
3 |
8 |
11 |
NM_168860.1 |
CG32425-RA, transcript variant A (CG32425), mRNA |
| 0 |
0 |
3 |
8 |
11 |
NM_168861.1 |
CG32425-RE, transcript variant E (CG32425), mRNA |
| 0 |
0 |
3 |
8 |
11 |
NM_168863.1 |
CG32425-RC, transcript variant C (CG32425), mRNA |
| 0 |
0 |
3 |
7 |
16 |
NM_136134.4 |
CG10186-RA, transcript variant A (CG10186), mRNA |
| 0 |
0 |
3 |
7 |
16 |
NM_165306.1 |
CG10186-RC, transcript variant C (CG10186), mRNA |
| 0 |
0 |
3 |
7 |
6 |
NM_130624.2 |
CG16903-RA (CG16903), mRNA |
| 0 |
0 |
3 |
5 |
13 |
NM_206784.1 |
CG6103-RE, transcript variant E (CrebB-17A), mRNA |
| 0 |
0 |
3 |
5 |
13 |
NM_206782.1 |
CG6103-RD, transcript variant D (CrebB-17A), mRNA |
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme03040
:
Spliceosome
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