|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10275R-1 |
| Symbol |
perd |
Full Name |
perdido |
| CG No |
CG10275 |
Old CG No |
CG10275 |
| Synonyms |
CT28869, perd, CG10275 |
| Accession No (Link to NCBI) |
NM_136037.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Chu WC, Hayashi S.<br> Mechano-chemical enforcement of tendon apical ECM into nano-filaments during Drosophila flight muscle development.<br> Curr Biol (2021) 31(7) 1366-1378.e7
[
PubMed ID = 33545042
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Weitkunat M, Kaya-Çopur A, Grill SW, Schnorrer F.<br> Tension and force-resistant attachment are essential for myofibrillogenesis in Drosophila flight muscle.<br> Curr Biol (2014) 24(7) 705-16
[
PubMed ID = 24631244
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCACTTCGGGACATTTCTGGG |
| Primer Seq. 3' |
aatctagaggtaccTCGTGGCATCGTCGAGGCTTA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCACTTCGGG ACATTTCTGG GAGGCGTGGG GGACTTTACG GCCGAGTTCT TGGATGATGT
0061 TATCGGTTTT CGGGGCTGTA TATCAGATGT CTTTTACAAC AACATCAACA TCATCAAACG
0121 TGCCAAGGAT CGCACGAGTC ACACCACCAG TACTGGAGTT GCCTGGACTT GCAGCACTGA
0181 GTTCGAAGGG TCCATTCAGG ACAGCATTAG TTTCATGAGG AACGATTCGT ATTCCCTGAT
0241 GATGAAAGAA TCGTATGCTA TGGGAGAAAC CCTTTCCTTG CAATTCCGCA CAATGGCCAG
0301 TGCTGGGGTT ATTTTCTTCA ACGGCGGCTT TGATTTCATT CTCCTCGAAA TAGAGGATCA
0361 ACACTTGAAG GTGACCTTCA ACAAGGCCGG CAGTCTGGTG CAGTTCATGA CAAATGAGCA
0421 TATATCCGAT GGCAAATGGC ATCGGGTCTT CTTGCGCTAT AACGCCGCCA TTGCGGAGCT
0481 AAGCCTCGAC GATGCCACGA |
| in silico PCR Fragment |
0001 CCACTTCGGG ACATTTCTGG GAGGCGTGGG GGACTTTACG GCCGAGTTCT TGGATGATGT
0061 TATCGGCTTT CGGGGCTGTA TATCAGATGT CTTTTACAAC AACATAAACA TCATCAAACG
0121 CGCCAAGGAT CGCACGAGTC ACACCACCAG TACTGGAGTT GCCTGGACTT GCAGCACTGA
0181 GTTCGAAGGG TCCATTCAGG ACAGCATTAG CTTCATGAGG AACGATTCGT ATTCCTTGAT
0241 GATGAAAGAA TCGTATGCTA TGGGAGAAAC CCTTTCCTTG CAATTCCGCA CAATGGCCAG
0301 TGCTGGGGTT ATTTTCTTCA ACGGCGGCTA TGATTTCATT CTCCTCGAAA TAGAGGATCA
0361 GCACTTGAAG GTGACCTTCA ACAAGGCAGG CAGTCTGGTG CAGTTCATGA CAAATGAGCA
0421 CATATCCGAT GGCAAATGGC ATCGGGTCTT CTTGCGCTAT AACGCCGCAA TTGCGGAGCT
0481 AAGCCTCGAC GATGCCACGA |
| Assemble Data |
<PRE>
10275R-1.IR_full 1 CCACTTCGGGACATTTCTGGGAGGCGTGGGGGACTTTACGGCCGAGTTCTTGGATGATGT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10275R-1.in silico 1 CCACTTCGGGACATTTCTGGGAGGCGTGGGGGACTTTACGGCCGAGTTCTTGGATGATGT 60
10275R-1.IR_full 61 TATCGG<span class="snp"><tt>T</tt></span>TTTCGGGGCTGTATATCAGATGTCTTTTACAACAACAT<span class="snp"><tt>C</tt></span>AACATCATCAAACG 120
||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||
10275R-1.in silico 61 TATCGG<span class="snp"><tt>C</tt></span>TTTCGGGGCTGTATATCAGATGTCTTTTACAACAACAT<span class="snp"><tt>A</tt></span>AACATCATCAAACG 120
10275R-1.IR_full 121 <span class="snp"><tt>T</tt></span>GCCAAGGATCGCACGAGTCACACCACCAGTACTGGAGTTGCCTGGACTTGCAGCACTGA 180
<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10275R-1.in silico 121 <span class="snp"><tt>C</tt></span>GCCAAGGATCGCACGAGTCACACCACCAGTACTGGAGTTGCCTGGACTTGCAGCACTGA 180
10275R-1.IR_full 181 GTTCGAAGGGTCCATTCAGGACAGCATTAG<span class="snp"><tt>T</tt></span>TTCATGAGGAACGATTCGTATTCC<span class="snp"><tt>C</tt></span>TGAT 240
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||
10275R-1.in silico 181 GTTCGAAGGGTCCATTCAGGACAGCATTAG<span class="snp"><tt>C</tt></span>TTCATGAGGAACGATTCGTATTCC<span class="snp"><tt>T</tt></span>TGAT 240
10275R-1.IR_full 241 GATGAAAGAATCGTATGCTATGGGAGAAACCCTTTCCTTGCAATTCCGCACAATGGCCAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10275R-1.in silico 241 GATGAAAGAATCGTATGCTATGGGAGAAACCCTTTCCTTGCAATTCCGCACAATGGCCAG 300
10275R-1.IR_full 301 TGCTGGGGTTATTTTCTTCAACGGCGGCT<span class="snp"><tt>T</tt></span>TGATTTCATTCTCCTCGAAATAGAGGATCA 360
|||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||
10275R-1.in silico 301 TGCTGGGGTTATTTTCTTCAACGGCGGCT<span class="snp"><tt>A</tt></span>TGATTTCATTCTCCTCGAAATAGAGGATCA 360
10275R-1.IR_full 361 <span class="snp"><tt>A</tt></span>CACTTGAAGGTGACCTTCAACAAGGC<span class="snp"><tt>C</tt></span>GGCAGTCTGGTGCAGTTCATGACAAATGAGCA 420
<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
10275R-1.in silico 361 <span class="snp"><tt>G</tt></span>CACTTGAAGGTGACCTTCAACAAGGC<span class="snp"><tt>A</tt></span>GGCAGTCTGGTGCAGTTCATGACAAATGAGCA 420
10275R-1.IR_full 421 <span class="snp"><tt>T</tt></span>ATATCCGATGGCAAATGGCATCGGGTCTTCTTGCGCTATAACGCCGC<span class="snp"><tt>C</tt></span>ATTGCGGAGCT 480
<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||
10275R-1.in silico 421 <span class="snp"><tt>C</tt></span>ATATCCGATGGCAAATGGCATCGGGTCTTCTTGCGCTATAACGCCGC<span class="snp"><tt>A</tt></span>ATTGCGGAGCT 480
10275R-1.IR_full 481 AAGCCTCGACGATGCCACGA 500
||||||||||||||||||||
10275R-1.in silico 481 AAGCCTCGACGATGCCACGA 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
11 |
NM_136037.2 |
CG10275-RA (CG10275), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_057962.4 |
CG1782-RA (Uba1), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_206256.1 |
CG33233-RA, transcript variant A (CG33233), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_137109.2 |
CG8613-RA (CG8613), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166583.1 |
CG3082-RB, transcript variant B (l(2)k09913), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137907.2 |
CG3082-RC, transcript variant C (l(2)k09913), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166582.1 |
CG3082-RA, transcript variant A (l(2)k09913), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166584.1 |
CG3082-RD, transcript variant D (l(2)k09913), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_165794.2 |
CG11895-RA (stan), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079561.2 |
CG9755-RC, transcript variant C (pum), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169259.1 |
CG9755-RD, transcript variant D (pum), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169258.1 |
CG9755-RA, transcript variant A (pum), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137975.1 |
CG30183-RA (CG30183), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_206067.1 |
CG1429-RF, transcript variant F (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057670.2 |
CG1429-RC, transcript variant C (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057672.2 |
CG1429-RD, transcript variant D (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057673.2 |
CG1429-RA, transcript variant A (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057671.2 |
CG1429-RB, transcript variant B (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165722.1 |
CG1429-RE, transcript variant E (Mef2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057969.3 |
CG9635-RD, transcript variant D (RhoGEF2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206147.1 |
CG9635-RE, transcript variant E (RhoGEF2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206146.1 |
CG9635-RF, transcript variant F (RhoGEF2), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132559.1 |
CG2750-RA (CG2750), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165900.1 |
CG8830-RB, transcript variant B (CG8830), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136940.1 |
CG8830-RA, transcript variant A (CG8830), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142122.2 |
CG7832-RA (CG7832), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168440.2 |
CG32072-RA (CG32072), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_164374.1 |
CG18497-RA, transcript variant A (spen), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_079979.2 |
CG18497-RB, transcript variant B (spen), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206658.1 |
CG11265-RE, transcript variant E (CG11265), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
