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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10210R-2 |
| Symbol |
tst |
Full Name |
twister |
| CG No |
CG10210 |
Old CG No |
CG10210 |
| Synonyms |
SKI2, cg10210, Ski2, CG10210, tst, Ski2/tst |
| Accession No (Link to NCBI) |
NM_079741.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGCGCAACTATATCCTGCGA |
| Primer Seq. 3' |
aatctagaggtaccAGCTTCATCCACTCCTGGACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTGCGCAACT ATATCCTGCG ACCGGAGCTG TCCCTCCACA ATCCCCTGCC GGATGTTTTG
0061 CCCCGCAAAT TCGATCCGAT GCGCCTGTTG CATGCgCCCC GTTGCCCGGG AAGCACTAAG
0121 TTGGCTCCTC GCCGCGACCA GAGTGGTCAC ATACTTGAGT TCGTGGAATT GGACTTGGAG
0181 GCCGTGGGCG CCAATGCCAA CAACTCGATG TCCATGCAGC GGGAGCCGGG CTTGCTGGAA
0241 GACGCGACGC GTGGCTCCCA CTCCAATTTT CCCTTTTGGC CAGGCGGTTT CGATGAGCAG
0301 CGCCAGCAGA TCGCAGCTCT GGATGTCAGC AACTTTCAGT TCGGGGACAA GCTGCTAACG
0361 GTGCCACCGG GCTTCAGCTC TGGCTACGAC TTCTTCCAAT CGCAATCTGT AACACTGCCG
0421 CCTGTGGTCG CCGATCCCAG TAACGTTGAT CTATTGGAAA ACCTAGAACA GGATCTGGAC
0481 GTCCAGGAGT GGATGAAGCT |
| in silico PCR Fragment |
0001 CTGCGCAACT ATATCCTGCG ACCGGAGCTG TCCCTCCACA ATCCCCTGCC GGATGTTTTG
0061 CCCCGCAAAT TCGATCCGAT GCGCCTGTTG CATGCGCCCC GTTGCCCGGG AAGCACTAAG
0121 TTGGCTCCTC GCCGCGACCA GAGTGGTCAC ATACTTGAGT TCGTGGAATT GGACTTGGAG
0181 GCCGTGGGCG CCAATGCCAA CAACTCGATG TCCATGCAGC GGGAGCCGGG CTTGCTGGAA
0241 GACGCGACGC GTGGCTCCCA CTCCAATTTT CCCTTTTGGC CAGGCGGTTT CGATGAGCAG
0301 CGCCAGCAGA TCGCAGCTCT GGATGTCAGC AACTTTCAGT TCGGGGACAA GCTGCTAACG
0361 GTGCCACCGG GCTTCAGCTC TGGCTACGAC TTCTTCCAAT CGCAATCTGT AACACTGCCG
0421 CCTGTGGTCG CCGATCCCAG TAACGTTGAT CTATTGGAAA ACCTAGAACA GGATCTGGAC
0481 GTCCAGGAGT GGATGAAGCT |
| Assemble Data |
10210R-2.IR_full 1 CTGCGCAACTATATCCTGCGACCGGAGCTGTCCCTCCACAATCCCCTGCCGGATGTTTTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 1 CTGCGCAACTATATCCTGCGACCGGAGCTGTCCCTCCACAATCCCCTGCCGGATGTTTTG 60
10210R-2.IR_full 61 CCCCGCAAATTCGATCCGATGCGCCTGTTGCATGCGCCCCGTTGCCCGGGAAGCACTAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 61 CCCCGCAAATTCGATCCGATGCGCCTGTTGCATGCGCCCCGTTGCCCGGGAAGCACTAAG 120
10210R-2.IR_full 121 TTGGCTCCTCGCCGCGACCAGAGTGGTCACATACTTGAGTTCGTGGAATTGGACTTGGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 121 TTGGCTCCTCGCCGCGACCAGAGTGGTCACATACTTGAGTTCGTGGAATTGGACTTGGAG 180
10210R-2.IR_full 181 GCCGTGGGCGCCAATGCCAACAACTCGATGTCCATGCAGCGGGAGCCGGGCTTGCTGGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 181 GCCGTGGGCGCCAATGCCAACAACTCGATGTCCATGCAGCGGGAGCCGGGCTTGCTGGAA 240
10210R-2.IR_full 241 GACGCGACGCGTGGCTCCCACTCCAATTTTCCCTTTTGGCCAGGCGGTTTCGATGAGCAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 241 GACGCGACGCGTGGCTCCCACTCCAATTTTCCCTTTTGGCCAGGCGGTTTCGATGAGCAG 300
10210R-2.IR_full 301 CGCCAGCAGATCGCAGCTCTGGATGTCAGCAACTTTCAGTTCGGGGACAAGCTGCTAACG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 301 CGCCAGCAGATCGCAGCTCTGGATGTCAGCAACTTTCAGTTCGGGGACAAGCTGCTAACG 360
10210R-2.IR_full 361 GTGCCACCGGGCTTCAGCTCTGGCTACGACTTCTTCCAATCGCAATCTGTAACACTGCCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 361 GTGCCACCGGGCTTCAGCTCTGGCTACGACTTCTTCCAATCGCAATCTGTAACACTGCCG 420
10210R-2.IR_full 421 CCTGTGGTCGCCGATCCCAGTAACGTTGATCTATTGGAAAACCTAGAACAGGATCTGGAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10210R-2.in silico 421 CCTGTGGTCGCCGATCCCAGTAACGTTGATCTATTGGAAAACCTAGAACAGGATCTGGAC 480
10210R-2.IR_full 481 GTCCAGGAGTGGATGAAGCT 500
||||||||||||||||||||
10210R-2.in silico 481 GTCCAGGAGTGGATGAAGCT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079741.2 |
CG10210-RA (tst), mRNA |
| 0.2 |
1 |
2 |
4 |
4 |
NM_143435.2 |
CG2321-RA (CG2321), mRNA |
| 0.2 |
1 |
2 |
2 |
6 |
NM_135633.2 |
CG4636-RA (SCAR), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_001042889.1 |
CG6105-RC, transcript variant C (l(2)06225), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_142396.1 |
CG7397-RA (CG7397), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_134516.2 |
CG32529-RA, transcript variant A (CG32529), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141285.1 |
CG14670-RA (CG14670), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057462.3 |
CG1916-RA (Wnt2), mRNA |
| 0 |
0 |
1 |
7 |
17 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_136827.2 |
CG7745-RA (CG7745), mRNA |
| 0 |
0 |
1 |
4 |
12 |
NM_080325.3 |
CG6450-RC (lva), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_166696.1 |
CG9071-RB, transcript variant B (NaCP60E), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_079134.5 |
CG9071-RA, transcript variant A (NaCP60E), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_138089.2 |
CG11416-RA (ori), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_142644.1 |
CG5466-RA (CG5466), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_206579.1 |
CG2005-RC, transcript variant C (Ptp99A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_170409.1 |
CG2005-RA, transcript variant A (Ptp99A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001014683.1 |
CG2005-RD, transcript variant D (Ptp99A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_057468.2 |
CG2005-RB, transcript variant B (Ptp99A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143755.2 |
CG14813-RA (deltaCOP), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167991.1 |
CG15812-RB, transcript variant B (pfk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079173.4 |
CG15812-RA, transcript variant A (pfk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079593.3 |
CG17228-RC, transcript variant C (pros), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_176459.1 |
CG17228-RD, transcript variant D (pros), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_169386.1 |
CG17228-RA, transcript variant A (pros), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167275.1 |
CG32668-RA (CG32668), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132347.1 |
CG3099-RB (CG3099), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078599.3 |
CG18657-RA (NetA), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136235.2 |
CG9270-RA, transcript variant A (CG9270), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206019.1 |
CG9270-RB, transcript variant B (CG9270), mRNA |
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