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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10119R-1

Symbol

LamC

Full Name

Lamin C

CG No

CG10119

Old CG No

CG10119

Synonyms

LAMC, pG-IF, lamC, G-IF, CG10119, LamC

Accession No (Link to NCBI)

NM_079018.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Karling T, Weavers H. Immune cells adapt to confined environments in vivo to optimise nuclear plasticity for migration. EMBO Rep (2025) 26(5) 1238-1268 [ PubMed ID = 39915297 ] [ RRC reference ]

Kosakamoto H, Fujisawa Y, Obata F, Miura M. High expression of A-type lamin in the leading front is required for Drosophila thorax closure. Biochem Biophys Res Commun (2018) 499(2) 209-214 [ PubMed ID = 29559239 ] [ RRC reference ]

Uchino R, Nonaka YK, Horigome T, Sugiyama S, Furukawa K. Loss of Drosophila A-type lamin C initially causes tendon abnormality including disintegration of cytoskeleton and nuclear lamina in muscular defects. Dev Biol (2013) 373(1) 216-27 [ PubMed ID = 22982669 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCACGCCGCGTCACATTGAA

Primer Seq. 3'

aatctagaggtaccCGGCCAACGATTGGTTGTACT

Predicted Fragment Size

IR fragment full Seq

0001 AGCACGCCGC GTCACATTGA ACACACGCGT TTCGCGCGCC TCCACCTTCC ACGCCGGTGG 
0061 GCGGGGCATC CACCTCGTCA AGGGTGGGCG CCACCTCACC CACCTCGCCC ACGCGCACCA 
0121 GCCGGCAGCA GGTAGAAGGA GTGAACTGCA GCATTTGAAC GATCGCCTGG CCTGCTACAT 
0181 CGATCGCATG CGCAACCATG GAGAACGAGA ACAGCCGGCT CACCCAGGGA ACTGAATCTC 
0241 GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
0301 GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
0361 ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
0421 GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
0481 GGCAAGTACA ACCAATCGTT GGCCG

in silico PCR Fragment

0001 AGCACGCCGC GTCACATTGA ACACACGCGT TTCGCGCGCC TCCACC-TCC ACGCCGGTGG 
0061 GCGGGGCATC CACCTCGTCA AGGGTGGGCG CCACCTCACC CACCTCGCCC ACGCGCACCA 
0121 GCCGGCAGCA GG-AGAAGGA G-GAACTGCA GCATTTGAAC GATCGCCTGG CCTGCTACAT 
0181 CGATCGCATG CGCAACC-TG GAGAACGAGA ACAGCCGGCT CACCCA-GGA ACTGAATCTC 
0241 GCCCAGGATA CCGTCAACAG GGAGACCTCT AATCTGAAGG CGGTCTATGA GAAGGAGCTG 
0301 GCCGCCGCTC GCAAGCTGCT CGATGAGACC GCCAAAGAGA AGGCCAAGCT TGAGATCGAT 
0361 ATCAAGCGTC TGTGGGAGGA GAACGACGAC TTGAAGCCCA GACTCGACAA AAAGACGAAG 
0421 GAGGCTACCG TGGCAGAGAA CAATGCCCGC CTGTATGAGA ACCGCTACAA CGAGGTGAAT 
0481 GGCAAGTACA ACCAATCGTT GGCCG

Assemble Data

<PRE>

10119R-1.IR_full       1   AGCACGCCGCGTCACATTGAACACACGCGTTTCGCGCGCCTCCACC<span class="snp"><tt>T</tt></span>TCCACGCCGGTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
10119R-1.in silico     1   AGCACGCCGCGTCACATTGAACACACGCGTTTCGCGCGCCTCCACC<span class="snp"><tt>-</tt></span>TCCACGCCGGTGG 60


10119R-1.IR_full       61  GCGGGGCATCCACCTCGTCAAGGGTGGGCGCCACCTCACCCACCTCGCCCACGCGCACCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     61  GCGGGGCATCCACCTCGTCAAGGGTGGGCGCCACCTCACCCACCTCGCCCACGCGCACCA 120


10119R-1.IR_full       121 GCCGGCAGCAGG<span class="snp"><tt>T</tt></span>AGAAGGAG<span class="snp"><tt>T</tt></span>GAACTGCAGCATTTGAACGATCGCCTGGCCTGCTACAT 180
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     121 GCCGGCAGCAGG<span class="snp"><tt>-</tt></span>AGAAGGAG<span class="snp"><tt>-</tt></span>GAACTGCAGCATTTGAACGATCGCCTGGCCTGCTACAT 180


10119R-1.IR_full       181 CGATCGCATGCGCAACC<span class="snp"><tt>A</tt></span>TGGAGAACGAGAACAGCCGGCTCACCCA<span class="snp"><tt>G</tt></span>GGAACTGAATCTC 240
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
10119R-1.in silico     181 CGATCGCATGCGCAACC<span class="snp"><tt>-</tt></span>TGGAGAACGAGAACAGCCGGCTCACCCA<span class="snp"><tt>-</tt></span>GGAACTGAATCTC 240


10119R-1.IR_full       241 GCCCAGGATACCGTCAACAGGGAGACCTCTAATCTGAAGGCGGTCTATGAGAAGGAGCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     241 GCCCAGGATACCGTCAACAGGGAGACCTCTAATCTGAAGGCGGTCTATGAGAAGGAGCTG 300


10119R-1.IR_full       301 GCCGCCGCTCGCAAGCTGCTCGATGAGACCGCCAAAGAGAAGGCCAAGCTTGAGATCGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     301 GCCGCCGCTCGCAAGCTGCTCGATGAGACCGCCAAAGAGAAGGCCAAGCTTGAGATCGAT 360


10119R-1.IR_full       361 ATCAAGCGTCTGTGGGAGGAGAACGACGACTTGAAGCCCAGACTCGACAAAAAGACGAAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     361 ATCAAGCGTCTGTGGGAGGAGAACGACGACTTGAAGCCCAGACTCGACAAAAAGACGAAG 420


10119R-1.IR_full       421 GAGGCTACCGTGGCAGAGAACAATGCCCGCCTGTATGAGAACCGCTACAACGAGGTGAAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10119R-1.in silico     421 GAGGCTACCGTGGCAGAGAACAATGCCCGCCTGTATGAGAACCGCTACAACGAGGTGAAT 480


10119R-1.IR_full       481 GGCAAGTACAACCAATCGTTGGCCG 505
                           |||||||||||||||||||||||||
10119R-1.in silico     481 GGCAAGTACAACCAATCGTTGGCCG 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	5	NM_079018.2	CG10119-RA (LamC), mRNA
0.41	2	16	20	29	NM_057268.3	CG6944-RA (Lam), mRNA
0.41	2	3	5	2	NM_170095.1	CG31141-RA (CG31141), mRNA
0.2	1	3	2	6	NM_080334.2	CG7038-RA (mRpL30), mRNA
0	0	4	2	13	NM_001043297.1	CG3339-RB, transcript variant B (CG3339), mRNA
0	0	4	2	11	NM_143300.1	CG3339-RA, transcript variant A (CG3339), mRNA
0	0	4	2	7	NM_167364.1	CG15747-RA (CG15747), mRNA
0	0	3	5	5	NM_078544.2	CG1689-RA (lz), mRNA
0	0	3	4	5	NM_079593.3	CG17228-RC, transcript variant C (pros), mRNA
0	0	3	4	5	NM_176459.1	CG17228-RD, transcript variant D (pros), mRNA
0	0	3	4	5	NM_169386.1	CG17228-RA, transcript variant A (pros), mRNA
0	0	3	4	5	NM_079059.2	CG5519-RA (Gbp), mRNA
0	0	3	4	3	NM_134486.1	CG14221-RA (CG14221), mRNA
0	0	3	2	4	NM_079148.2	CG6883-RA, transcript variant A (trh), mRNA
0	0	3	2	4	NM_001043110.1	CG6883-RB, transcript variant B (trh), mRNA
0	0	3	2	3	NM_168712.2	CG6652-RB, transcript variant B (CG6652), mRNA
0	0	3	2	3	NM_140689.3	CG6652-RA, transcript variant A (CG6652), mRNA
0	0	3	2	2	NM_164497.1	CG2903-RA, transcript variant A (Hrs), mRNA
0	0	3	2	2	NM_164496.1	CG2903-RC, transcript variant C (Hrs), mRNA
0	0	3	2	2	NM_080360.3	CG2903-RB, transcript variant B (Hrs), mRNA
0	0	2	10	7	NM_001038881.1	CG3257-RB, transcript variant B (CG3257), mRNA
0	0	2	10	7	NM_138043.3	CG3257-RA, transcript variant A (CG3257), mRNA
0	0	2	9	2	NM_137107.1	CG13942-RA (CG13942), mRNA
0	0	2	5	10	NM_139422.2	CG1017-RA (CG1017), mRNA
0	0	2	5	8	NM_132380.1	CG15311-RA (CG15311), mRNA
0	0	2	4	10	NM_165220.1	CG6605-RA (BicD), mRNA
0	0	2	4	5	NM_139839.3	CG32381-RA (unc-13-4A), mRNA
0	0	2	4	2	NM_165918.1	CG30055-RA (CG30055), mRNA
0	0	2	2	10	NM_169685.1	CG8884-RC, transcript variant C (Sap47), mRNA
0	0	2	2	10	NM_169689.1	CG8884-RG, transcript variant G (Sap47), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04214 : Apoptosis - fly
dme04820 : Cytoskeleton in muscle cells

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