|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10079R-2 |
| Symbol |
Egfr |
Full Name |
Epidermal growth factor receptor |
| CG No |
CG10079 |
Old CG No |
CG10079 |
| Synonyms |
egfr, Elp-B1, EGFR, DER, dEGFR, flb, der, d-egf-r, top, torpedo/egfr, EgfR, CG10079, EGF-R, DER/EGFR, Der, D-EGFR, dEGFR1, Egf-r, Elp, l(2)09261, Egf, EK2-6, c-erbB, DER/torpedo, unnamed, DmHD-33, Elp-B1RB1, Elp-1, DER flb, top/DER, Torpedo/Egfr, D-Egf, Degfr, Torpedo/DER, torpedo/Egfr, EGFr, DER/faint little ball, DER/top, l(2)05351, top/flb, l(2)57DEFa, pnt, l(2)57Ea, l(2)57EFa, HD-33, El, C-erb, Egfr, EFG-R |
| Accession No (Link to NCBI) |
NM_057411.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ostalé CM, Pulido D, Vega-Cuesta P, López-Varea A, de Celis JF.<br> Developmental analysis of Spalt function in the Drosophila ring gland.<br> Development (2024)
[
PubMed ID = 39087588
]
[
RRC reference
]
Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Molnar C, Estrada B, de Celis JF.<br> Tay bridge and extracellular-regulated kinase activity are required for motoneuron function in the Drosophila neural system.<br> Genes Brain Behav (2018) 17(8) e12470
[
PubMed ID = 29524312
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Molnar C, de Celis JF.<br> Tay bridge is a negative regulator of EGFR signalling and interacts with Erk and Mkp3 in the Drosophila melanogaster wing.<br> PLoS Genet (2013) 9(12) e1003982
[
PubMed ID = 24348264
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Liu M, Lim TM, Cai Y.<br> The Drosophila female germline stem cell lineage acts to spatially restrict DPP function within the niche.<br> Sci Signal (2010) 3(132) ra57
[
PubMed ID = 20664066
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCTCCTGGCCCACTGCATTT |
| Primer Seq. 3' |
aatctagaggtaccCGTGCGTCCGCGAATGATTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGCTCCTGGC CCACTGCATT TGCATTTGGC CCGCGTCGgc GGCCCGCAGA TCGCTACGCC
0061 CGCCAGAACA ATCGCCAGCG CCATCAGGAT ATAGATCGCG ATCGGGATCG AGATCGATTC
0121 CTATACCGCA GCAGTTCGGC CCAAAATCGA CAGAGGGGCG GGGCCAACTN TCGCCCTGGG
0181 ACTGGGAGCC AACGGAGTCA CCATTCCCAC CAGTCTGGAG GATAAGAACA AGAACGAGTT
0241 CGTCAAGGGG AAAATCTGCA TCGGCACTAA ATCTCGGCTC TCCGTGCCCT CCAACAAGGA
0301 ACATCATTAC CGGAACCTCA GAGATCGGTA CACGAACTGT ACGTATGTGG ATGGCAACCT
0361 GGAGCTGACC TGGCTGCCCA ACGAGAATTT GGACCTCAGC TTCCTGGACA ACATACGGGA
0421 GGTCACCGGC TATATTCTGA TCAGTCATGT GGACGTTAAG AAAGTGGTAT TTCCCAAACT
0481 ACAAATCATT CgNGGACGCA CG |
| in silico PCR Fragment |
0001 TGCTCCTGGC CCACTGCATT TGCATTTGGC CCGCGTCGGC GGCCCGC-GA TCGCTACGCC
0061 CGCCAGAACA ATCGCCAGCG CCATCAGGAT ATAGATCGCG ATCGGGATCG AGATCGATTC
0121 CTATACCGCA GCAGTTCGGC CCAAAATCGA CAGAGGGGCG GGGCCAACT- TCGCCCTGGG
0181 ACTGGGAGCC AACGGAGTCA CCATTCCCAC CAGTCTGGAG GATAAGAACA AGAACGAGTT
0241 CGTCAAGGGG AAAATCTGCA TCGGCACTAA ATCTCGGCTC TCCGTGCCCT CCAACAAGGA
0301 ACATCATTAC CGGAACCTCA GAGATCGGTA CACGAACTGT ACGTATGTGG ATGGCAACCT
0361 GGAGCTGACC TGGCTGCCCA ACGAGAATTT GGACCTCAGC TTCCTGGACA ACATACGGGA
0421 GGTCACCGGC TATATTCTGA TCAGTCATGT GGACGTTAAG AAAGTGGTAT TTCCCAAACT
0481 ACAAATCATT CGCGGACGCA CG |
| Assemble Data |
<PRE>
10079R-2.IR_full 1 TGCTCCTGGCCCACTGCATTTGCATTTGGCCCGCGTCGGCGGCCCGC<span class="snp"><tt>A</tt></span>GATCGCTACGCC 60
|||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||
10079R-2.in silico 1 TGCTCCTGGCCCACTGCATTTGCATTTGGCCCGCGTCGGCGGCCCGC<span class="snp"><tt>-</tt></span>GATCGCTACGCC 60
10079R-2.IR_full 61 CGCCAGAACAATCGCCAGCGCCATCAGGATATAGATCGCGATCGGGATCGAGATCGATTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 61 CGCCAGAACAATCGCCAGCGCCATCAGGATATAGATCGCGATCGGGATCGAGATCGATTC 120
10079R-2.IR_full 121 CTATACCGCAGCAGTTCGGCCCAAAATCGACAGAGGGGCGGGGCCAACT<span class="snp"><tt>N</tt></span>TCGCCCTGGG 180
|||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||
10079R-2.in silico 121 CTATACCGCAGCAGTTCGGCCCAAAATCGACAGAGGGGCGGGGCCAACT<span class="snp"><tt>-</tt></span>TCGCCCTGGG 180
10079R-2.IR_full 181 ACTGGGAGCCAACGGAGTCACCATTCCCACCAGTCTGGAGGATAAGAACAAGAACGAGTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 181 ACTGGGAGCCAACGGAGTCACCATTCCCACCAGTCTGGAGGATAAGAACAAGAACGAGTT 240
10079R-2.IR_full 241 CGTCAAGGGGAAAATCTGCATCGGCACTAAATCTCGGCTCTCCGTGCCCTCCAACAAGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 241 CGTCAAGGGGAAAATCTGCATCGGCACTAAATCTCGGCTCTCCGTGCCCTCCAACAAGGA 300
10079R-2.IR_full 301 ACATCATTACCGGAACCTCAGAGATCGGTACACGAACTGTACGTATGTGGATGGCAACCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 301 ACATCATTACCGGAACCTCAGAGATCGGTACACGAACTGTACGTATGTGGATGGCAACCT 360
10079R-2.IR_full 361 GGAGCTGACCTGGCTGCCCAACGAGAATTTGGACCTCAGCTTCCTGGACAACATACGGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 361 GGAGCTGACCTGGCTGCCCAACGAGAATTTGGACCTCAGCTTCCTGGACAACATACGGGA 420
10079R-2.IR_full 421 GGTCACCGGCTATATTCTGATCAGTCATGTGGACGTTAAGAAAGTGGTATTTCCCAAACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10079R-2.in silico 421 GGTCACCGGCTATATTCTGATCAGTCATGTGGACGTTAAGAAAGTGGTATTTCCCAAACT 480
10079R-2.IR_full 481 ACAAATCATTCG<span class="snp"><tt>N</tt></span>GGACGCACG 502
||||||||||||<span class="snp"><tt> </tt></span>|||||||||
10079R-2.in silico 481 ACAAATCATTCG<span class="snp"><tt>C</tt></span>GGACGCACG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
7 |
NM_057411.3 |
CG10079-RB, transcript variant B (Egfr), mRNA |
| 47.71 |
230 |
5 |
1 |
4 |
NM_057410.3 |
CG10079-RA, transcript variant A (Egfr), mRNA |
| 0 |
0 |
5 |
7 |
11 |
NM_166877.1 |
CG32811-RA (CG32811), mRNA |
| 0 |
0 |
4 |
10 |
15 |
NM_132959.2 |
CG8949-RA (CG8949), mRNA |
| 0 |
0 |
4 |
8 |
35 |
NM_134738.2 |
CG4887-RA (CG4887), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_169701.2 |
CG31045-RB, transcript variant B (Mhcl), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_001043251.1 |
CG31045-RF, transcript variant F (Mhcl), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_001043250.1 |
CG31045-RG, transcript variant G (Mhcl), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_169700.2 |
CG31045-RA, transcript variant A (Mhcl), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_206509.1 |
CG8053-RB, transcript variant B (eIF-1A), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_079989.2 |
CG8053-RA, transcript variant A (eIF-1A), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_170566.1 |
CG1856-RE, transcript variant E (ttk), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_170565.1 |
CG1856-RB, transcript variant B (ttk), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_170564.1 |
CG1856-RA, transcript variant A (ttk), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_165349.2 |
CG9339-RG, transcript variant G (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_206016.1 |
CG9339-RH, transcript variant H (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_136229.3 |
CG9339-RA, transcript variant A (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_165348.2 |
CG9339-RE, transcript variant E (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_206017.1 |
CG9339-RD, transcript variant D (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_165350.2 |
CG9339-RB, transcript variant B (CG9339), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_143159.1 |
CG5028-RA (CG5028), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_170568.1 |
CG1856-RF, transcript variant F (ttk), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_080172.2 |
CG1856-RC, transcript variant C (ttk), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_170567.1 |
CG1856-RD, transcript variant D (ttk), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001038813.1 |
CG5983-RB, transcript variant B (ACXC), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_135749.2 |
CG5983-RA, transcript variant A (ACXC), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141187.3 |
CG14643-RA (CG14643), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_143965.1 |
CG14310-RB, transcript variant B (CG14310), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04013
:
MAPK signaling pathway - fly
dme04068
:
FoxO signaling pathway
dme04144
:
Endocytosis
dme04214
:
Apoptosis - fly
dme04320
:
Dorso-ventral axis formation
|
|
|
