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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10067R-2 |
| Symbol |
Act57B |
Full Name |
Actin 57B |
| CG No |
CG10067 |
Old CG No |
CG10067 |
| Synonyms |
Actin, actin, CG10067, T10, C, CG30294, act 57A, M, act57A, Act57A, BEST:RE38067, anon-EST:CL2c12, Act57B |
| Accession No (Link to NCBI) |
NM_079076.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.<br> Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.<br> PLoS One (2017) 12(3) e0172951
[
PubMed ID = 28257485
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGAAGTTGCTGCTCTGGTCGT |
| Primer Seq. 3' |
aatctagaggtaccCATAACCCTCATAGATGGGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGAAGTTGCT GCTCTGGTCG TTGACAATGG CTCCGGCATG TGCAAGGCCg GTTTCGCCGG
0061 TGATGACGCT CCCCGTGCCG TCTTCCCCTC CATCGTCGGT CGTCCACGCC ACCAGGGTGT
0121 GATGGTCGGT ATGGGCCAGA AGGACTCGTA CGTCGGTGAT GAGGCCCAGT CCAAGCGTGG
0181 TATCCTCACC CTGAAATACC CCATCGAGCA CGGTATCATC ACCAACTGGG ATGATATGGA
0241 GAAGATCTGG CACCACACCT TCTACAACGA GCTGCGTGTT GCCCCCGAGG AGCACCCCGT
0301 CCTGCTGACT GAGGCCCCCC TGAACCCCAA GGCTAACCGC GAGAAGATGA CCCAGATCAT
0361 GTTTGAGACC TTCAACTCGC CCGCCATGTA CGTCGCCATC CAGGCCGTGC TCTCCCTGTA
0421 CGCCTCCGGT CGTACCACCG GTATCGTTCT GGACTCCGGT GATGGTGTCT CCCACACCGT
0481 CCCCATCTAT GAGGGTTATG |
| in silico PCR Fragment |
0001 TGAAGTTGCT GCTCTGGTCG TTGACAATGG CTCCGGCATG TGCAAGGCCG GTTTCGCCGG
0061 TGATGACGCT CCCCGTGCCG TCTTCCCCTC CATCGTCGGT CGTCCACGCC ACCAGGGTGT
0121 GATGGTCGGT ATGGGCCAGA AGGACTCGTA CGTCGGTGAT GAGGCCCAGT CCAAGCGTGG
0181 TATCCTCACC CTGAAATACC CCATCGAGCA CGGTATCATC ACCAACTGGG ATGATATGGA
0241 GAAGATCTGG CACCACACCT TCTACAACGA GCTGCGTGTT GCCCCCGAGG AGCACCCCGT
0301 CCTGCTGACT GAGGCCCCCC TGAACCCCAA GGCTAACCGC GAGAAGATGA CCCAGATCAT
0361 GTTTGAGACC TTCAACTCGC CCGCCATGTA CGTCGCCATC CAGGCCGTGC TCTCCCTGTA
0421 CGCCTCCGGT CGTACCACCG GTATCGTTCT GGACTCCGGT GATGGTGTCT CCCACACCGT
0481 CCCCATCTAT GAGGGTTATG |
| Assemble Data |
<PRE>
10067R-2.IR_full 1 TGAAGTTGCTGCTCTGGTCGTTGACAATGGCTCCGGCATGTGCAAGGCCGGTTTCGCCGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 1 TGAAGTTGCTGCTCTGGTCGTTGACAATGGCTCCGGCATGTGCAAGGCCGGTTTCGCCGG 60
10067R-2.IR_full 61 TGATGACGCTCCCCGTGCCGTCTTCCCCTCCATCGTCGGTCGTCCACGCCACCAGGGTGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 61 TGATGACGCTCCCCGTGCCGTCTTCCCCTCCATCGTCGGTCGTCCACGCCACCAGGGTGT 120
10067R-2.IR_full 121 GATGGTCGGTATGGGCCAGAAGGACTCGTACGTCGGTGATGAGGCCCAGTCCAAGCGTGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 121 GATGGTCGGTATGGGCCAGAAGGACTCGTACGTCGGTGATGAGGCCCAGTCCAAGCGTGG 180
10067R-2.IR_full 181 TATCCTCACCCTGAAATACCCCATCGAGCACGGTATCATCACCAACTGGGATGATATGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 181 TATCCTCACCCTGAAATACCCCATCGAGCACGGTATCATCACCAACTGGGATGATATGGA 240
10067R-2.IR_full 241 GAAGATCTGGCACCACACCTTCTACAACGAGCTGCGTGTTGCCCCCGAGGAGCACCCCGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 241 GAAGATCTGGCACCACACCTTCTACAACGAGCTGCGTGTTGCCCCCGAGGAGCACCCCGT 300
10067R-2.IR_full 301 CCTGCTGACTGAGGCCCCCCTGAACCCCAAGGCTAACCGCGAGAAGATGACCCAGATCAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 301 CCTGCTGACTGAGGCCCCCCTGAACCCCAAGGCTAACCGCGAGAAGATGACCCAGATCAT 360
10067R-2.IR_full 361 GTTTGAGACCTTCAACTCGCCCGCCATGTACGTCGCCATCCAGGCCGTGCTCTCCCTGTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 361 GTTTGAGACCTTCAACTCGCCCGCCATGTACGTCGCCATCCAGGCCGTGCTCTCCCTGTA 420
10067R-2.IR_full 421 CGCCTCCGGTCGTACCACCGGTATCGTTCTGGACTCCGGTGATGGTGTCTCCCACACCGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-2.in silico 421 CGCCTCCGGTCGTACCACCGGTATCGTTCTGGACTCCGGTGATGGTGTCTCCCACACCGT 480
10067R-2.IR_full 481 CCCCATCTATGAGGGTTATG 500
||||||||||||||||||||
10067R-2.in silico 481 CCCCATCTATGAGGGTTATG 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079076.3 |
CG10067-RA, transcript variant A (Act57B), mRNA |
| 96.68 |
466 |
2 |
3 |
2 |
NM_166414.1 |
CG10067-RB, transcript variant B (Act57B), mRNA |
| 13.07 |
63 |
158 |
154 |
65 |
NM_001014726.1 |
CG4027-RC, transcript variant C (Act5C), mRNA |
| 13.07 |
63 |
158 |
154 |
65 |
NM_001014725.1 |
CG4027-RD, transcript variant D (Act5C), mRNA |
| 13.07 |
63 |
158 |
154 |
65 |
NM_167053.1 |
CG4027-RA, transcript variant A (Act5C), mRNA |
| 13.07 |
63 |
158 |
154 |
65 |
NM_078497.2 |
CG4027-RB, transcript variant B (Act5C), mRNA |
| 11.61 |
56 |
126 |
142 |
105 |
NM_169525.1 |
CG18290-RB, transcript variant B (Act87E), mRNA |
| 11.61 |
56 |
126 |
142 |
105 |
NM_057743.3 |
CG18290-RA, transcript variant A (Act87E), mRNA |
| 11.41 |
55 |
148 |
132 |
69 |
NM_079643.1 |
CG5178-RA (Act88F), mRNA |
| 10.58 |
51 |
158 |
102 |
95 |
NM_079486.3 |
CG7478-RA (Act79B), mRNA |
| 0.2 |
1 |
3 |
4 |
2 |
NM_143725.2 |
CG18039-RA (KaiRIA), mRNA |
| 0 |
0 |
40 |
159 |
149 |
NM_078901.2 |
CG12051-RA (Act42A), mRNA |
| 0 |
0 |
3 |
13 |
52 |
NM_057689.1 |
CG5409-RA (Arp53D), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_057627.3 |
CG8996-RB, transcript variant B (wal), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_165847.1 |
CG8996-RA, transcript variant A (wal), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_141038.2 |
CG9389-RA (CG9389), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_057258.2 |
CG10699-RA, transcript variant A (Lim3), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165277.1 |
CG10699-RB, transcript variant B (Lim3), mRNA |
| 0 |
0 |
1 |
8 |
4 |
NM_078648.2 |
CG9901-RA (Arp14D), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_168072.1 |
CG15010-RA, transcript variant A (ago), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_168073.1 |
CG15010-RB, transcript variant B (ago), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_079198.2 |
CG15010-RC, transcript variant C (ago), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001038734.1 |
CG16902-RC (Hr4), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001042804.1 |
CG2174-RB, transcript variant B (Myo10A), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_132441.1 |
CG2174-RA, transcript variant A (Myo10A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001042881.1 |
CG8486-RC, transcript variant C (CG8486), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164795.2 |
CG8486-RB, transcript variant B (CG8486), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_135344.2 |
CG8486-RA, transcript variant A (CG8486), mRNA |
| 0 |
0 |
0 |
9 |
26 |
NM_079607.2 |
CG6174-RA (Arp87C), mRNA |
| 0 |
0 |
0 |
9 |
20 |
NM_079244.2 |
CG7558-RA (Arp66B), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
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|
