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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9804R-1

Symbol

CG9804

Full Name

CG9804

CG No

CG9804

Old CG No

CG9804

Synonyms

CG9804

Accession No (Link to NCBI)

NM_141215.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

male pupal lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGCCTTTTGTCCGACCGCTG

Primer Seq. 3'

aatctagaggtaccTTGAGGCCGATTCCATGCGTG

Predicted Fragment Size

IR fragment full Seq

0001 ATGCCTTTTG TCCGACCGCT GGTGACCGTG GTGCGGGCCG GGCGGCATAG CTATTCAGCA 
0061 GGATTACAGC TACAGCAACG TCTTGCAAGG TCCAACCAGA TCCTGAATCC GCCAGCGGAG 
0121 TTCCGAAACT ACCTGGTGCT TCAGGAGCAC GACCCGGTCT ACACAGTCGG ACTAAGGACA 
0181 AAGGACTATA CGGCGCAGGA TGAAGACCGA CTCCGCCGGC TAGGAGCAGA CTTCCATCGC 
0241 ACAGATCGCG GTGGCTTGAT CACATTTCAC GGCCCCGGCC AGTTGGTGGC CTATCCCATT 
0301 CTGCACCTGG GTCAGTTCGT GCCGAGCATC CGCTGGTACG TGGCGACGTT GGAGCGGATG 
0361 GTTGTCGAGG CCTGCCACCA GATGGGCATT TCCAGTGCCA AGGCGACCAA GGACACCGGT 
0421 ATCTGGGTGG GTGACAACAA GATCTGTGCC ATCGGTATCC ACGGCTCGCG TTACGTCACC 
0481 ACGCATGGAA TCGGCCTCAA

in silico PCR Fragment

0001 ATGCCTTTTG TCCGACCGCT GGTGACCGTG GTGCGGGCCG GGCGGCATAG CTATTCAGCA 
0061 GGATTACAGC TACAGCAACG TCTTGCAAGG TCCAACCAGA TCCTGAATCC GCCAGCGGAG 
0121 TTCCGAAACT ACCTGGTGCT TCAGGAGCAC GACCCGGTCT ACACAGTCGG ACTAAGGACA 
0181 AAGGACTATA CGGCGCAGGA TGAAGACCGA CTCCGCCGGC TAGGAGCAGA CTTCCATCGC 
0241 ACAGATCGCG GTGGCTTGAT CACATTTCAC GGCCCCGGCC AGTTGGTGGC CTATCCCATT 
0301 CTGCACCTGG GTCAGTTCGT GCCGAGCATC CGCTGGTACG TGGCGACGTT GGAGCGGATG 
0361 GTTGTCGAGG CCTGCCACCA GATGGGCATT TCCAGTGCCA AGGCGACCAA GGACACCGGT 
0421 ATCTGGGTGG GTGACAACAA GATCTGTGCC ATCGGTATCC ACGGCTCGCG TTACGTCACC 
0481 ACGCATGGAA TCGGCCTCAA

Assemble Data


9804R-1.IR_full       1   ATGCCTTTTGTCCGACCGCTGGTGACCGTGGTGCGGGCCGGGCGGCATAGCTATTCAGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     1   ATGCCTTTTGTCCGACCGCTGGTGACCGTGGTGCGGGCCGGGCGGCATAGCTATTCAGCA 60


9804R-1.IR_full       61  GGATTACAGCTACAGCAACGTCTTGCAAGGTCCAACCAGATCCTGAATCCGCCAGCGGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     61  GGATTACAGCTACAGCAACGTCTTGCAAGGTCCAACCAGATCCTGAATCCGCCAGCGGAG 120


9804R-1.IR_full       121 TTCCGAAACTACCTGGTGCTTCAGGAGCACGACCCGGTCTACACAGTCGGACTAAGGACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     121 TTCCGAAACTACCTGGTGCTTCAGGAGCACGACCCGGTCTACACAGTCGGACTAAGGACA 180


9804R-1.IR_full       181 AAGGACTATACGGCGCAGGATGAAGACCGACTCCGCCGGCTAGGAGCAGACTTCCATCGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     181 AAGGACTATACGGCGCAGGATGAAGACCGACTCCGCCGGCTAGGAGCAGACTTCCATCGC 240


9804R-1.IR_full       241 ACAGATCGCGGTGGCTTGATCACATTTCACGGCCCCGGCCAGTTGGTGGCCTATCCCATT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     241 ACAGATCGCGGTGGCTTGATCACATTTCACGGCCCCGGCCAGTTGGTGGCCTATCCCATT 300


9804R-1.IR_full       301 CTGCACCTGGGTCAGTTCGTGCCGAGCATCCGCTGGTACGTGGCGACGTTGGAGCGGATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     301 CTGCACCTGGGTCAGTTCGTGCCGAGCATCCGCTGGTACGTGGCGACGTTGGAGCGGATG 360


9804R-1.IR_full       361 GTTGTCGAGGCCTGCCACCAGATGGGCATTTCCAGTGCCAAGGCGACCAAGGACACCGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     361 GTTGTCGAGGCCTGCCACCAGATGGGCATTTCCAGTGCCAAGGCGACCAAGGACACCGGT 420


9804R-1.IR_full       421 ATCTGGGTGGGTGACAACAAGATCTGTGCCATCGGTATCCACGGCTCGCGTTACGTCACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9804R-1.in silico     421 ATCTGGGTGGGTGACAACAAGATCTGTGCCATCGGTATCCACGGCTCGCGTTACGTCACC 480


9804R-1.IR_full       481 ACGCATGGAATCGGCCTCAA 500
                          ||||||||||||||||||||
9804R-1.in silico     481 ACGCATGGAATCGGCCTCAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_141215.2	CG9804-RA (CG9804), mRNA
0	0	2	4	4	NM_079067.2	CG11949-RA, transcript variant A (cora), mRNA
0	0	2	3	2	NM_166337.1	CG11949-RB, transcript variant B (cora), mRNA
0	0	2	2	3	NM_001014481.1	CG7147-RC, transcript variant C (kuz), mRNA
0	0	2	2	3	NM_165047.1	CG7147-RB, transcript variant B (kuz), mRNA
0	0	2	2	3	NM_057839.3	CG7147-RA, transcript variant A (kuz), mRNA
0	0	2	2	2	NM_136436.1	CG11125-RA (CG11125), mRNA
0	0	2	2	2	NM_134830.1	CG3597-RA (CG3597), mRNA
0	0	1	3	7	NM_165581.1	CG11198-RB, transcript variant B (CG11198), mRNA
0	0	1	3	7	NM_136498.1	CG11198-RA, transcript variant A (CG11198), mRNA
0	0	1	3	6	NM_057806.3	CG13279-RA (Cyt-b5-r), mRNA
0	0	1	3	6	NM_168696.2	CG11905-RF, transcript variant F (CG11905), mRNA
0	0	1	3	6	NM_168695.2	CG11905-RB, transcript variant B (CG11905), mRNA
0	0	1	3	4	NM_169625.1	CG4264-RB, transcript variant B (Hsc70-4), mRNA
0	0	1	3	4	NM_176503.1	CG4264-RF, transcript variant F (Hsc70-4), mRNA
0	0	1	3	4	NM_169627.1	CG4264-RD, transcript variant D (Hsc70-4), mRNA
0	0	1	3	4	NM_169626.1	CG4264-RC, transcript variant C (Hsc70-4), mRNA
0	0	1	3	4	NM_176502.1	CG4264-RE, transcript variant E (Hsc70-4), mRNA
0	0	1	3	4	NM_079632.4	CG4264-RA, transcript variant A (Hsc70-4), mRNA
0	0	0	5	7	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	0	5	7	NM_001038743.1	CG33980-RA (CG33980), mRNA
0	0	0	5	4	NM_168162.1	CG10173-RA (Best2), mRNA
0	0	0	5	4	NM_136960.2	CG8785-RA, transcript variant A (CG8785), mRNA
0	0	0	5	4	NM_165932.1	CG8785-RB, transcript variant B (CG8785), mRNA
0	0	0	5	2	NM_167028.1	CG6775-RB, transcript variant B (rg), mRNA
0	0	0	5	2	NM_080023.1	CG6775-RA, transcript variant A (rg), mRNA
0	0	0	5	2	NM_001042796.1	CG6775-RC, transcript variant C (rg), mRNA
0	0	0	4	4	NM_168368.1	CG32044-RA (CG32044), mRNA
0	0	0	4	4	NM_079449.2	CG7978-RA (Ac76E), mRNA
0	0	0	4	3	NM_079733.3	CG4677-RA, transcript variant A (lmd), mRNA

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