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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9780R-1

Symbol

CG9780

Full Name

CG9780

CG No

CG9780

Old CG No

CG9780

Synonyms

CG9780

Accession No (Link to NCBI)

NM_141192.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCCCTGCCAGGACTTCTACG

Primer Seq. 3'

aatctagaggtaccACTGCGCTCCAAGCTGAGCAC

Predicted Fragment Size

IR fragment full Seq

0001 AGCCCTGCCA GGACTTCTAC GGTTATGTCT GCTCGCACAG CGCCAACCTC AGCGAGACTG 
0061 GCAGGCAGCG ATTCCAAAGC TTGCTTAAGG AAACGGACGT GGCGCAGCTG NATGGACATG 
0121 GAACTCCAAC TGATAAACTT CTTCAAGTCT TGCGAGAGCG GGCGTGGCGT AGAGGCGCTC 
0181 AAGGGATCTC AATTGTATAG GCAGAGTGGG GGCTGGCCTA GCNTTGGAAC CGAACAAAGT 
0241 CAATGCCAGT CAGCGGACTA ACCAAACCCA AAGATGGCTG GGACTTCTTG GGTACTTCCA 
0301 CGANAGTGGG CGCGCCCNTA TTTCTTCGAG ACGTCGGTCA CCATGCAGTC CAACAAGCGA 
0361 CTTGTGGTAC TGCAACCCNG ACGGCACGCG CCGAAACACT CTAAGAAAAT TCGAGCAGCG 
0421 GGTGGGCGAA GTCCTGCAAA GCTTCGGGAT AGAACAGAGC CGCGCCCACG TGACCGCCCT 
0481 CGAAGTGCTC AGCTTGGAGC GCAGT

in silico PCR Fragment

0001 AGCCCTGCCA GGACTTCTAC GGTTATGTCT GCTCGCACAG CGCCAACCTC AGCGAGACTG 
0061 GCAGGCAGCG ATTCCAAAGC TTGCTTAAGG AAACGGACGT GGCGCAGCTG -ATGGACATG 
0121 GAACTCCAAC TGATAAACTT CTTCAAGTCT TGCGAGAGCG GGCGTGGCGT AGAGGCGCTC 
0181 AAGGGATCTC AATTGTATAG GCAGAGTGGG GGCTGGCCTA GC-TTGGAAC CGAACAAAGT 
0241 CAATGCCAGT CAGCGGACTA ACCAAACCCA AAGATGGCTG GGACTTCTTG GGTACTTCCA 
0301 CGA-AGTGGG CGCGCCC-TA TTTCTTCGAG ACGTCGGTCA CCATGCAGTC CAACAAGCGA 
0361 CTTGTGGTAC TGCAACCC-G ACGGCACGCG CCGAAACACT CTAAGAAAAT TCGAGCAGCG 
0421 GGTGGGCGAA GTCCTGCAAA GCTTCGGGAT AGAACAGAGC CGCGCCCACG TGACCGCCCT 
0481 CGAAGTGCTC AGCTTGGAGC GCAGT

Assemble Data

<PRE>

9780R-1.IR_full       1   AGCCCTGCCAGGACTTCTACGGTTATGTCTGCTCGCACAGCGCCAACCTCAGCGAGACTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     1   AGCCCTGCCAGGACTTCTACGGTTATGTCTGCTCGCACAGCGCCAACCTCAGCGAGACTG 60


9780R-1.IR_full       61  GCAGGCAGCGATTCCAAAGCTTGCTTAAGGAAACGGACGTGGCGCAGCTG<span class="snp"><tt>N</tt></span>ATGGACATG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
9780R-1.in silico     61  GCAGGCAGCGATTCCAAAGCTTGCTTAAGGAAACGGACGTGGCGCAGCTG<span class="snp"><tt>-</tt></span>ATGGACATG 120


9780R-1.IR_full       121 GAACTCCAACTGATAAACTTCTTCAAGTCTTGCGAGAGCGGGCGTGGCGTAGAGGCGCTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     121 GAACTCCAACTGATAAACTTCTTCAAGTCTTGCGAGAGCGGGCGTGGCGTAGAGGCGCTC 180


9780R-1.IR_full       181 AAGGGATCTCAATTGTATAGGCAGAGTGGGGGCTGGCCTAGC<span class="snp"><tt>N</tt></span>TTGGAACCGAACAAAGT 240
                          ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
9780R-1.in silico     181 AAGGGATCTCAATTGTATAGGCAGAGTGGGGGCTGGCCTAGC<span class="snp"><tt>-</tt></span>TTGGAACCGAACAAAGT 240


9780R-1.IR_full       241 CAATGCCAGTCAGCGGACTAACCAAACCCAAAGATGGCTGGGACTTCTTGGGTACTTCCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     241 CAATGCCAGTCAGCGGACTAACCAAACCCAAAGATGGCTGGGACTTCTTGGGTACTTCCA 300


9780R-1.IR_full       301 CGA<span class="snp"><tt>N</tt></span>AGTGGGCGCGCCC<span class="snp"><tt>N</tt></span>TATTTCTTCGAGACGTCGGTCACCATGCAGTCCAACAAGCGA 360
                          |||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     301 CGA<span class="snp"><tt>-</tt></span>AGTGGGCGCGCCC<span class="snp"><tt>-</tt></span>TATTTCTTCGAGACGTCGGTCACCATGCAGTCCAACAAGCGA 360


9780R-1.IR_full       361 CTTGTGGTACTGCAACCC<span class="snp"><tt>N</tt></span>GACGGCACGCGCCGAAACACTCTAAGAAAATTCGAGCAGCG 420
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     361 CTTGTGGTACTGCAACCC<span class="snp"><tt>-</tt></span>GACGGCACGCGCCGAAACACTCTAAGAAAATTCGAGCAGCG 420


9780R-1.IR_full       421 GGTGGGCGAAGTCCTGCAAAGCTTCGGGATAGAACAGAGCCGCGCCCACGTGACCGCCCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9780R-1.in silico     421 GGTGGGCGAAGTCCTGCAAAGCTTCGGGATAGAACAGAGCCGCGCCCACGTGACCGCCCT 480


9780R-1.IR_full       481 CGAAGTGCTCAGCTTGGAGCGCAGT 505
                          |||||||||||||||||||||||||
9780R-1.in silico     481 CGAAGTGCTCAGCTTGGAGCGCAGT 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_176395.1	CG9780-RB, transcript variant B (CG9780), mRNA
100	482	0	0	0	NM_141192.2	CG9780-RA, transcript variant A (CG9780), mRNA
0	0	2	2	3	NM_167897.1	CG12004-RB, transcript variant B (CG12004), mRNA
0	0	2	2	3	NM_139370.2	CG12004-RA, transcript variant A (CG12004), mRNA
0	0	2	2	2	NM_057445.2	CG1046-RA (zen), mRNA
0	0	1	5	3	NM_141793.1	CG4820-RA (CG4820), mRNA
0	0	1	5	3	NM_143344.2	CG5520-RA (Gp93), mRNA
0	0	1	4	4	NM_143668.2	CG1981-RA (Thd1), mRNA
0	0	1	3	3	NM_132335.2	CG12139-RB (CG12139), mRNA
0	0	1	3	2	NM_206184.1	CG13425-RC, transcript variant C (bl), mRNA
0	0	1	3	2	NM_145883.2	CG13425-RB, transcript variant B (bl), mRNA
0	0	1	3	2	NM_206185.1	CG13425-RA, transcript variant A (bl), mRNA
0	0	1	3	2	NM_206183.1	CG13425-RD, transcript variant D (bl), mRNA
0	0	1	2	3	NM_134982.2	CG18013-RA (Psf2), mRNA
0	0	1	1	2	NM_079000.2	CG3879-RA (Mdr49), mRNA
0	0	0	5	3	NM_142590.1	CG4465-RA (CG4465), mRNA
0	0	0	5	2	NM_080377.2	CG10207-RA (NaPi-T), mRNA
0	0	0	4	4	NM_079992.3	CG17976-RA (sut3), mRNA
0	0	0	4	3	NM_166851.1	CG17829-RB, transcript variant B (CG17829), mRNA
0	0	0	4	3	NM_166852.1	CG17829-RC, transcript variant C (CG17829), mRNA
0	0	0	4	3	NM_143797.1	CG17829-RA, transcript variant A (CG17829), mRNA
0	0	0	4	2	NM_167469.2	CG10545-RB, transcript variant B (Gbeta13F), mRNA
0	0	0	4	2	NM_080351.5	CG10545-RA, transcript variant A (Gbeta13F), mRNA
0	0	0	4	2	NM_206737.1	CG10545-RE, transcript variant E (Gbeta13F), mRNA
0	0	0	4	2	NM_206739.1	CG10545-RC, transcript variant C (Gbeta13F), mRNA
0	0	0	4	2	NM_206738.1	CG10545-RD, transcript variant D (Gbeta13F), mRNA
0	0	0	4	2	NM_206736.1	CG10545-RF, transcript variant F (Gbeta13F), mRNA
0	0	0	3	11	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	0	3	10	NM_168156.1	CG7018-RA, transcript variant A (Ets65A), mRNA
0	0	0	3	10	NM_079221.2	CG7018-RB, transcript variant B (Ets65A), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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