NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9762R-3

Symbol

l(3)neo18

Full Name

lethal (3) neo18

CG No

CG9762

Old CG No

CG9762

Synonyms

CG9762, l(3)neo18

Accession No (Link to NCBI)

NM_143785.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Garcia CJ, Khajeh J, Coulanges E, Chen EI, Owusu-Ansah E. Regulation of Mitochondrial Complex I Biogenesis in Drosophila Flight Muscles. Cell Rep (2017) 20(1) 264-278 [ PubMed ID = 28683319 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K. A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction. PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Owusu-Ansah E, Song W, Perrimon N. Muscle mitohormesis promotes longevity via systemic repression of insulin signaling. Cell (2013) 155(3) 699-712 [ PubMed ID = 24243023 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGTCGGTTGGAGCCGTTTG

Primer Seq. 3'

aatctagaggtaccCCACCGATGGTCGGTAGTAGT

Predicted Fragment Size

IR fragment full Seq

0001 ATGGTCGGTT GGAGCCGTTT GCTGTCGCcg gCGGCNANNT TCGCCAGCTA CCGGGCTGTC 
0061 CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAANCAG CTGCGCAGAA TGGGAGGCGA 
0121 TCATGGACAC CACCAAATGA TCATCAAGCC ATCCCGCTTC CAGTGGGACA AGTTCAAGGA 
0181 TCTGTTGCAC TTTTACGTAA TGCTCGGCGT CATCCCAGTC ACCGCATTGG TTCTCTACGC 
0241 GAACATCTTT GTGGGACCCG CGCAGCTCGC CGAGATTNCC CGAGGGCTAT GAGCCCAAGC 
0301 ACTGGGAGTA CGAGAAGCAC CCCATCTCGC GCTTTATTTC CCGCTACATT TTGAACTCGG 
0361 ATCAGCAAAA CTACGAGAAA TCCCTGCACT ATCTCTACGA GGAGAACGAA AAAGCCCAGA 
0421 TTCGACTCCT TGAGGACGAA GTACGTCGCA AGATGTCGGA GCGCAATGAT TACCAGGCCT 
0481 ACTACTACCG ACCATCGGTG G

in silico PCR Fragment

0001 ATGGTCGGTT GGAGCCGTTT GCTGTCGCCG GCGGCGAAGT TCGCCAGCTA CCGGGCTGTC 
0061 CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAA-CAG CTGCGCAGAA TGGGAGGCGA 
0121 TCATGGACAC CACCAAATGA TCATCAAGCC ATCCCGCTTC CAGTGGGACA AGTTCAAGGA 
0181 TCTGTTGCAC TTTTACGTAA TGCTCGGCGT CATCCCAGTC ACCGCATTGG TTCTCTACGC 
0241 GAACATCTTT GTGGGACCCG CGCAGCTCGC CGAGATT-CC CGAGGGCTAT GAGCCCAAGC 
0301 ACTGGGAGTA CGAGAAGCAC CCCATCTCGC GCTTTATTTC CCGCTACATT TTGAACTCGG 
0361 ATCAGCAAAA CTACGAGAAA TCCCTGCACT ATCTCTACGA GGAGAACGAA AAAGCCCAGA 
0421 TTCGACTCCT TGAGGACGAA GTACGTCGCA AGATGTCGGA GCGCAATGAT TACCAGGCCT 
0481 ACTACTACCG ACCATCGGTG G

Assemble Data

<PRE>

9762R-3.IR_full       1   ATGGTCGGTTGGAGCCGTTTGCTGTCGCCGGCGGC<span class="snp"><tt>N</tt></span>A<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>TTCGCCAGCTACCGGGCTGTC 60
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
9762R-3.in silico     1   ATGGTCGGTTGGAGCCGTTTGCTGTCGCCGGCGGC<span class="snp"><tt>G</tt></span>A<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span>TTCGCCAGCTACCGGGCTGTC 60


9762R-3.IR_full       61  CTGCAAGAGCCCGCGTGCCGGAATGCCCTGCACCAA<span class="snp"><tt>N</tt></span>CAGCTGCGCAGAATGGGAGGCGA 120
                          ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
9762R-3.in silico     61  CTGCAAGAGCCCGCGTGCCGGAATGCCCTGCACCAA<span class="snp"><tt>-</tt></span>CAGCTGCGCAGAATGGGAGGCGA 120


9762R-3.IR_full       121 TCATGGACACCACCAAATGATCATCAAGCCATCCCGCTTCCAGTGGGACAAGTTCAAGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9762R-3.in silico     121 TCATGGACACCACCAAATGATCATCAAGCCATCCCGCTTCCAGTGGGACAAGTTCAAGGA 180


9762R-3.IR_full       181 TCTGTTGCACTTTTACGTAATGCTCGGCGTCATCCCAGTCACCGCATTGGTTCTCTACGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9762R-3.in silico     181 TCTGTTGCACTTTTACGTAATGCTCGGCGTCATCCCAGTCACCGCATTGGTTCTCTACGC 240


9762R-3.IR_full       241 GAACATCTTTGTGGGACCCGCGCAGCTCGCCGAGATT<span class="snp"><tt>N</tt></span>CCCGAGGGCTATGAGCCCAAGC 300
                          |||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
9762R-3.in silico     241 GAACATCTTTGTGGGACCCGCGCAGCTCGCCGAGATT<span class="snp"><tt>-</tt></span>CCCGAGGGCTATGAGCCCAAGC 300


9762R-3.IR_full       301 ACTGGGAGTACGAGAAGCACCCCATCTCGCGCTTTATTTCCCGCTACATTTTGAACTCGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9762R-3.in silico     301 ACTGGGAGTACGAGAAGCACCCCATCTCGCGCTTTATTTCCCGCTACATTTTGAACTCGG 360


9762R-3.IR_full       361 ATCAGCAAAACTACGAGAAATCCCTGCACTATCTCTACGAGGAGAACGAAAAAGCCCAGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9762R-3.in silico     361 ATCAGCAAAACTACGAGAAATCCCTGCACTATCTCTACGAGGAGAACGAAAAAGCCCAGA 420


9762R-3.IR_full       421 TTCGACTCCTTGAGGACGAAGTACGTCGCAAGATGTCGGAGCGCAATGATTACCAGGCCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9762R-3.in silico     421 TTCGACTCCTTGAGGACGAAGTACGTCGCAAGATGTCGGAGCGCAATGATTACCAGGCCT 480


9762R-3.IR_full       481 ACTACTACCGACCATCGGTGG 501
                          |||||||||||||||||||||
9762R-3.in silico     481 ACTACTACCGACCATCGGTGG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	481	0	0	2	NM_143785.2	CG9762-RA (l(3)neo18), mRNA
0	0	3	3	6	NM_143004.1	CG13615-RA (CG13615), mRNA
0	0	2	4	8	NM_136277.2	CG1416-RA, transcript variant A (CG1416), mRNA
0	0	2	4	8	NM_165398.1	CG1416-RB, transcript variant B (CG1416), mRNA
0	0	2	4	8	NM_165399.1	CG1416-RC, transcript variant C (CG1416), mRNA
0	0	2	3	5	NM_142106.1	CG14843-RA (CG14843), mRNA
0	0	2	3	2	NM_079541.2	CG1089-RA (alpha-Est5), mRNA
0	0	2	2	5	NM_080192.2	CG10449-RA (Catsup), mRNA
0	0	2	2	4	NM_079974.2	CG3870-RA (chrw), mRNA
0	0	2	2	2	NM_135019.1	CG15627-RA (CG15627), mRNA
0	0	1	5	2	NM_169551.2	CG31469-RA (CG31469), mRNA
0	0	1	4	4	NM_164653.1	CG31646-RA (CG31646), mRNA
0	0	1	3	4	NM_141198.3	CG9776-RA, transcript variant A (CG9776), mRNA
0	0	1	3	4	NM_164339.3	CG9776-RB, transcript variant B (CG9776), mRNA
0	0	1	2	2	NM_001015127.1	CG41056-PA.3 (CG41056), mRNA
0	0	1	2	2	NM_132819.2	CG8105-RA (CG8105), mRNA
0	0	1	2	2	NM_136076.2	CG10621-RA (CG10621), mRNA
0	0	0	9	8	NM_141572.1	CG11768-RA (CG11768), mRNA
0	0	0	6	8	NM_080358.2	CG1484-RA (fliI), mRNA
0	0	0	6	7	NM_166790.2	CG32019-RA, transcript variant A (bt), mRNA
0	0	0	5	9	NM_132522.2	CG32662-RA (CG32662), mRNA
0	0	0	5	5	NM_205877.1	CG32019-RD, transcript variant D (bt), mRNA
0	0	0	5	5	NM_205875.1	CG32019-RE, transcript variant E (bt), mRNA
0	0	0	5	4	NM_205876.1	CG32019-RC, transcript variant C (bt), mRNA
0	0	0	5	2	NM_170006.2	CG31169-RB, transcript variant B (CG31169), mRNA
0	0	0	5	2	NM_170005.1	CG31169-RA, transcript variant A (CG31169), mRNA
0	0	0	4	11	NM_164923.1	CG31716-RE, transcript variant E (CG31716), mRNA
0	0	0	4	11	NM_164921.1	CG31716-RC, transcript variant C (CG31716), mRNA
0	0	0	4	11	NM_164922.1	CG31716-RD, transcript variant D (CG31716), mRNA
0	0	0	4	11	NM_164919.1	CG31716-RA, transcript variant A (CG31716), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00190 : Oxidative phosphorylation
dme01100 : Metabolic pathways

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