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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9648R-4 |
| Symbol |
Max |
Full Name |
Max |
| CG No |
CG9648 |
Old CG No |
CG9648 |
| Synonyms |
dMax, max, dmax, CG9648, Max |
| Accession No (Link to NCBI) |
NM_140840.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCGCGACATAGACATCGAAA |
| Primer Seq. 3' |
aatctagaggtaccTATGCGTGGAAGGTCTTCATC |
| Predicted Fragment Size |
466 |
| IR fragment full Seq |
0001 ACCGCGACAT AGACATCGAA AGTGACGAGG ACGGGGACTC GGACACTGGC TTGGGATCCT
0061 CGCGACACAC AAACACCGCC AATTTCACCC AGGCCGAAAA GCGGGCGCAC CACAACGCTT
0121 TGGAGCGAAA GGCGGCGCGA TCACATCAAG GAGAGCTTCA CCAACCTGCG TGAGGCGGTG
0181 CCCACACTCA AGGGCGAAAA GGCGAGTCGA GCTCAAATCC TGAAAAAGAC CACCGAATGC
0241 ATACAGACGA TGAGGCGGAA GATCAGTGAA AACCAGAAAG ACATCGAAGA GATCAAGAGG
0301 CAAAACAACA TAATAGCTAA GCAGATACAA GCACTGGAGA GTTCAAATGG GGATCAGTTC
0361 AGCGAATTTC TCAGCGACGA GGAAGTGGGC AGCGAGGAGG CCGACGACGA AGACCTCGAC
0421 CAGGACTTCA GTAGACGCAA TAAAAAGATG AAGACCTTCC ACGCATA |
| in silico PCR Fragment |
0001 ACCGCGACAT AGACATCGAA AGTGACGAGG ACGGGGACTC GGACACTGGC TTGGGATCCT
0061 CGCGACACAC AAACACCGCC AATTTCACCC AGGCCGAAAA GCGGGCGCAC CACAACGCTT
0121 TGGAGCG-AA GGCGGCGCGA TCACATCAAG GAGAGCTTCA CCAACCTGCG TGAGGCGGTG
0181 CCCACACTCA AGGGCGAAAA GGCGAGTCGA GCTCAAATCC TGAAAAAGAC CACCGAATGC
0241 ATACAGACGA TGAGGCGGAA GATCAGTGAA AACCAGAAAG ACATCGAAGA GATCAAGAGG
0301 CAAAACAACA TAATAGCTAA GCAGATACAA GCACTGGAGA GTTCAAATGG GGATCAGTTC
0361 AGCGAATTTC TCAGCGACGA GGAAGTGGGC AGCGAGGAGG CCGACGACGA AGACCTCGAC
0421 CAGGACTTCA GTAGACGCAA TAAAAAGATG AAGACCTTCC ACGCATA |
| Assemble Data |
<PRE>
9648R-4.IR_full 1 ACCGCGACATAGACATCGAAAGTGACGAGGACGGGGACTCGGACACTGGCTTGGGATCCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 1 ACCGCGACATAGACATCGAAAGTGACGAGGACGGGGACTCGGACACTGGCTTGGGATCCT 60
9648R-4.IR_full 61 CGCGACACACAAACACCGCCAATTTCACCCAGGCCGAAAAGCGGGCGCACCACAACGCTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 61 CGCGACACACAAACACCGCCAATTTCACCCAGGCCGAAAAGCGGGCGCACCACAACGCTT 120
9648R-4.IR_full 121 TGGAGCG<span class="snp"><tt>A</tt></span>AAGGCGGCGCGATCACATCAAGGAGAGCTTCACCAACCTGCGTGAGGCGGTG 180
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 121 TGGAGCG<span class="snp"><tt>-</tt></span>AAGGCGGCGCGATCACATCAAGGAGAGCTTCACCAACCTGCGTGAGGCGGTG 180
9648R-4.IR_full 181 CCCACACTCAAGGGCGAAAAGGCGAGTCGAGCTCAAATCCTGAAAAAGACCACCGAATGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 181 CCCACACTCAAGGGCGAAAAGGCGAGTCGAGCTCAAATCCTGAAAAAGACCACCGAATGC 240
9648R-4.IR_full 241 ATACAGACGATGAGGCGGAAGATCAGTGAAAACCAGAAAGACATCGAAGAGATCAAGAGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 241 ATACAGACGATGAGGCGGAAGATCAGTGAAAACCAGAAAGACATCGAAGAGATCAAGAGG 300
9648R-4.IR_full 301 CAAAACAACATAATAGCTAAGCAGATACAAGCACTGGAGAGTTCAAATGGGGATCAGTTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 301 CAAAACAACATAATAGCTAAGCAGATACAAGCACTGGAGAGTTCAAATGGGGATCAGTTC 360
9648R-4.IR_full 361 AGCGAATTTCTCAGCGACGAGGAAGTGGGCAGCGAGGAGGCCGACGACGAAGACCTCGAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 361 AGCGAATTTCTCAGCGACGAGGAAGTGGGCAGCGAGGAGGCCGACGACGAAGACCTCGAC 420
9648R-4.IR_full 421 CAGGACTTCAGTAGACGCAATAAAAAGATGAAGACCTTCCACGCATA 467
|||||||||||||||||||||||||||||||||||||||||||||||
9648R-4.in silico 421 CAGGACTTCAGTAGACGCAATAAAAAGATGAAGACCTTCCACGCATA 467
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
448 |
0 |
0 |
0 |
NM_140840.2 |
CG9648-RA (Max), mRNA |
| 12.94 |
58 |
4 |
4 |
4 |
NM_140841.2 |
CG9666-RA (CG9666), mRNA |
| 0 |
0 |
4 |
4 |
12 |
NM_080365.2 |
CG4894-RB, transcript variant B (Ca-alpha1D), mRNA |
| 0 |
0 |
4 |
4 |
12 |
NM_165146.1 |
CG4894-RC, transcript variant C (Ca-alpha1D), mRNA |
| 0 |
0 |
4 |
4 |
12 |
NM_134429.2 |
CG4894-RA, transcript variant A (Ca-alpha1D), mRNA |
| 0 |
0 |
4 |
4 |
12 |
NM_165147.1 |
CG4894-RD, transcript variant D (Ca-alpha1D), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_078835.2 |
CG12283-RA (kek1), mRNA |
| 0 |
0 |
2 |
6 |
8 |
NM_206382.1 |
CG5151-RB, transcript variant B (CG5151), mRNA |
| 0 |
0 |
2 |
6 |
8 |
NM_140587.1 |
CG5151-RA, transcript variant A (CG5151), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_166199.2 |
CG5935-RA, transcript variant A (Dek), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_206139.2 |
CG5935-RD, transcript variant D (Dek), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_166198.1 |
CG5935-RC, transcript variant C (Dek), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_143801.2 |
CG5935-RB, transcript variant B (Dek), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142868.1 |
CG13830-RA (CG13830), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_137994.2 |
CG5554-RA (CG5554), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_135257.1 |
CG4496-RA (CG4496), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_169402.2 |
CG5344-RB, transcript variant B (wkd), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_141832.2 |
CG5344-RA, transcript variant A (wkd), mRNA |
| 0 |
0 |
1 |
3 |
16 |
NM_132267.4 |
CG12109-RB (Caf1-180), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_144377.1 |
CG3706-RA (CG3706), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_057576.3 |
CG6407-RA (Wnt5), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078986.2 |
CG8472-RA, transcript variant A (Cam), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165870.1 |
CG8472-RB, transcript variant B (Cam), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137605.2 |
CG13872-RA (CG13872), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134625.2 |
CG17600-RA, transcript variant A (CG17600), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167787.1 |
CG17600-RB, transcript variant B (CG17600), mRNA |
| 0 |
0 |
0 |
7 |
23 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_140883.1 |
CG8780-RA (tey), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_079930.2 |
CG3856-RB, transcript variant B (Oamb), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_169913.1 |
CG3856-RA, transcript variant A (Oamb), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme03083
:
Polycomb repressive complex
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