NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9619R-3

Symbol

CG9619

Full Name

CG9619

CG No

CG9619

Old CG No

CG9619

Synonyms

149039_at, CG9619

Accession No (Link to NCBI)

NM_140847.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGAGTCTGTCCTCCAAGCTG

Primer Seq. 3'

aatctagaggtaccTCATCCTGGAAAGCTGGCTGC

Predicted Fragment Size

IR fragment full Seq

0001 CGGAGNCTGT CCTCCAAGCT GCgNACCTTG GGATCACAGA CAACCGAGGA GGGCGAGGGC 
0061 AACNCGGAGG ATGAGCCGAT CATAAATGGA ACCAGCACGA ACACGTGGGT CAACTCGCAC 
0121 GACTCGGAGC AGACGGTGAC GGACCTGCAG CCACTGCGCC ATGAGTCCGA CTCCTTCTTC 
0181 GACTTCGACT GCGAGCTGGA GTCGCCGGGG AGTCCTGTGG ACGAGTGCGA GTACCTGCGC 
0241 TTGATCGAAA CTGCCTCGAA TACGCCGCAC AACTCTACGG CGGAGTCGGG CTATGCCTGC 
0301 GCTTCAATGG CCAGCAGCCA TAGTAGCAGC AATGATGGTC CGTACTTTGA TGCATCCGCT 
0361 TCCACATCCA CAGCTGCAGC TGCCCAGGAT CAGACGCTGC CAGCCTCGGA ACACAAAGAG 
0421 TATGTAAATA CCCTACAACT AAACGGAAAG CATGGACTGG CCAATGGAGT GCAGGAGGAG 
0481 CAGCCAGCTT TCCAGGATGA

in silico PCR Fragment

0001 CGGAGTCTGT CCTCCAAGCT GCGCACCTTG GGATCACAGA CAACCGAGGA GGGCGAGGGC 
0061 AACGCGGAGG ATGAGCCGAT CATAAATGGA ACCAGCACGA ACACGTGGGT CAACTCGCAC 
0121 GACTCGGAGC AGACGGTGAC GGACCTGCAG CCACTGCGCC ATGAGTCCGA CTCCTTCTTC 
0181 GACTTCGACT GCGAGCTGGA GTCGCCGGGG AGTCCTGTGG ACGAGTGCGA GTACCTGCGC 
0241 TTGATCGAAA CTGCCTCGAA TACGCCGCAC AACTCTACGG CGGAGTCGGG CTATGCCTGC 
0301 GCTTCAATGG CCAGCAGCCA TAGTAGCAGC AATGATGGTC CGTACTTTGA TGCATCCGCT 
0361 TCCACATCCA CAGCTGCAGC TGCCCAGGAT CAGACGCTGC CAGCCTCGGA ACACAAAGAG 
0421 TATGTAAATA CCCTACAACT AAACGGAAAG CATGGACTGG CCAATGGAGT GCAGGAGGAG 
0481 CAGCCAGCTT TCCAGGATGA

Assemble Data

<PRE>

9619R-3.IR_full       1   CGGAG<span class="snp"><tt>N</tt></span>CTGTCCTCCAAGCTGCG<span class="snp"><tt>N</tt></span>ACCTTGGGATCACAGACAACCGAGGAGGGCGAGGGC 60
                          |||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||
9619R-3.in silico     1   CGGAG<span class="snp"><tt>T</tt></span>CTGTCCTCCAAGCTGCG<span class="snp"><tt>C</tt></span>ACCTTGGGATCACAGACAACCGAGGAGGGCGAGGGC 60


9619R-3.IR_full       61  AAC<span class="snp"><tt>N</tt></span>CGGAGGATGAGCCGATCATAAATGGAACCAGCACGAACACGTGGGTCAACTCGCAC 120
                          |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     61  AAC<span class="snp"><tt>G</tt></span>CGGAGGATGAGCCGATCATAAATGGAACCAGCACGAACACGTGGGTCAACTCGCAC 120


9619R-3.IR_full       121 GACTCGGAGCAGACGGTGACGGACCTGCAGCCACTGCGCCATGAGTCCGACTCCTTCTTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     121 GACTCGGAGCAGACGGTGACGGACCTGCAGCCACTGCGCCATGAGTCCGACTCCTTCTTC 180


9619R-3.IR_full       181 GACTTCGACTGCGAGCTGGAGTCGCCGGGGAGTCCTGTGGACGAGTGCGAGTACCTGCGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     181 GACTTCGACTGCGAGCTGGAGTCGCCGGGGAGTCCTGTGGACGAGTGCGAGTACCTGCGC 240


9619R-3.IR_full       241 TTGATCGAAACTGCCTCGAATACGCCGCACAACTCTACGGCGGAGTCGGGCTATGCCTGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     241 TTGATCGAAACTGCCTCGAATACGCCGCACAACTCTACGGCGGAGTCGGGCTATGCCTGC 300


9619R-3.IR_full       301 GCTTCAATGGCCAGCAGCCATAGTAGCAGCAATGATGGTCCGTACTTTGATGCATCCGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     301 GCTTCAATGGCCAGCAGCCATAGTAGCAGCAATGATGGTCCGTACTTTGATGCATCCGCT 360


9619R-3.IR_full       361 TCCACATCCACAGCTGCAGCTGCCCAGGATCAGACGCTGCCAGCCTCGGAACACAAAGAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     361 TCCACATCCACAGCTGCAGCTGCCCAGGATCAGACGCTGCCAGCCTCGGAACACAAAGAG 420


9619R-3.IR_full       421 TATGTAAATACCCTACAACTAAACGGAAAGCATGGACTGGCCAATGGAGTGCAGGAGGAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9619R-3.in silico     421 TATGTAAATACCCTACAACTAAACGGAAAGCATGGACTGGCCAATGGAGTGCAGGAGGAG 480


9619R-3.IR_full       481 CAGCCAGCTTTCCAGGATGA 500
                          ||||||||||||||||||||
9619R-3.in silico     481 CAGCCAGCTTTCCAGGATGA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_140847.1	CG9619-RA (CG9619), mRNA
0.2	1	2	5	10	NM_001042819.1	CG5424-RF, transcript variant F (f), mRNA
0.2	1	2	5	9	NM_176746.1	CG5424-RC, transcript variant C (f), mRNA
0	0	5	3	11	NM_136485.2	CG30377-RA (CG30377), mRNA
0	0	3	6	21	NM_167965.1	CG32296-RA (Mrtf), mRNA
0	0	3	4	6	NM_140896.1	CG8334-RA (CG8334), mRNA
0	0	2	4	6	NM_139922.2	CG13676-RA (CG13676), mRNA
0	0	2	4	3	NM_057251.3	CG3647-RB, transcript variant B (stc), mRNA
0	0	2	4	3	NM_057250.3	CG3647-RA, transcript variant A (stc), mRNA
0	0	2	3	9	NM_080049.2	CG11614-RA (nkd), mRNA
0	0	2	3	5	NM_133083.1	CG15040-RA (CG15040), mRNA
0	0	2	3	2	NM_143010.1	CG13623-RA (CG13623), mRNA
0	0	2	2	11	NM_001042894.1	CG5461-RF, transcript variant F (bun), mRNA
0	0	2	2	11	NM_080364.3	CG5461-RA, transcript variant A (bun), mRNA
0	0	2	2	9	NM_080125.2	CG4399-RB (east), mRNA
0	0	2	2	8	NM_137385.1	CG18432-RA (CG18432), mRNA
0	0	1	7	8	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	1	7	8	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	1	7	7	NM_206564.1	CG5794-RE, transcript variant E (CG5794), mRNA
0	0	1	5	2	NM_057313.3	CG3736-RA (okr), mRNA
0	0	1	5	2	NM_058024.3	CG18642-RA (Bem46), mRNA
0	0	1	4	5	NM_140828.2	CG3797-RA (CG3797), mRNA
0	0	1	3	4	NM_135241.1	CG9211-RA (iHog), mRNA
0	0	1	3	2	NM_134519.1	CG11940-RA, transcript variant A (CG11940), mRNA
0	0	1	3	2	NM_137836.1	CG12489-RA (Dnr1), mRNA
0	0	1	2	9	NM_206089.1	CG33473-RB (luna), mRNA
0	0	1	2	7	NM_135266.3	CG5149-RA (CG5149), mRNA
0	0	1	2	6	NM_170259.2	CG31324-RA (CG31324), mRNA
0	0	1	2	4	NM_142396.1	CG7397-RA (CG7397), mRNA
0	0	0	6	21	NM_132004.2	CG4136-RA (CG4136), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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