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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9486R-1 |
| Symbol |
CG9486 |
Full Name |
CG9486 |
| CG No |
CG9486 |
Old CG No |
CG9486 |
| Synonyms |
CG9486 |
| Accession No (Link to NCBI) |
NM_135161.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCCGAGGATCTGGAGAGGGA |
| Primer Seq. 3' |
aatctagaggtaccTATGGCCACGACACTGGCGGA |
| Predicted Fragment Size |
403 |
| IR fragment full Seq |
0001 ACCCGAGGAT CTGGAGAGGG AGTACCAGGA GGCTGAGCAG AAGGAGATCA CGTGCCTGCT
0061 GGACAAGATA CACAAGTTTC TGGCCGGGAT CGAGCGGCAG GCCAATATCT TCAAGCACTA
0121 CGGAGTGGAG CGGGCGCTTT ACCTGTACAT GCTCGGTGTG GACGTGTCCA TTCGACGCCA
0181 GAGAGTGGGC ACCCGCCTGG TGGAGGCCAC CATTGAGCTG GGTCGCCAGC GTGGATTTCC
0241 CGTCGTCACA TCTACCTGCA GCAACCAGAA CTCCAAACGC CTTATGACCG CCCTAAACAT
0301 GGAGTGCATA CTCACAAAGG ATTACGCGGA CTACAAGGAC GAGCACGGGG AGATCGTCCT
0361 GCGGGCATCC GAGCCACACA CCTCCGCCAG TGTCGTGGCC ATA |
| in silico PCR Fragment |
0001 ACCCGAGGAT CTGGAGAGGG AGTACCAGGA GGCTGAGCAG AAGGAGATCA CGTGCCTGCT
0061 GGACAAGATA CACAAGTTTC TGGCCGGGAT CGAGCGGCAG GCCAATATCT TCAAGCACTA
0121 CGGAGTGGAG CGGGCGCTTT ACCTGTACAT GCTCGGTGTG GACGTGTCCA TTCGACGCCA
0181 GAGAGTGGGC ACCCGCCTGG TGGAGGCCAC CATTGAGCTG GGTCGCCAGC GTGGATTTCC
0241 CGTCGTCACA TCTACCTGCA GCAACCAGAA CTCCAAACGC CTTATGACCG CCCTAAACAT
0301 GGAGTGCATA CTCACAAAGG ATTACGCGGA CTACAAGGAC GAGCACGGGG AGATCGTCCT
0361 GCGGGCATCC GAGCCACACA CCTCCGCCAG TGTCGTGGCC ATA |
| Assemble Data |
<PRE>
9486R-1.IR_full 1 ACCCGAGGATCTGGAGAGGGAGTACCAGGAGGCTGAGCAGAAGGAGATCACGTGCCTGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 1 ACCCGAGGATCTGGAGAGGGAGTACCAGGAGGCTGAGCAGAAGGAGATCACGTGCCTGCT 60
9486R-1.IR_full 61 GGACAAGATACACAAGTTTCTGGCCGGGATCGAGCGGCAGGCCAATATCTTCAAGCACTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 61 GGACAAGATACACAAGTTTCTGGCCGGGATCGAGCGGCAGGCCAATATCTTCAAGCACTA 120
9486R-1.IR_full 121 CGGAGTGGAGCGGGCGCTTTACCTGTACATGCTCGGTGTGGACGTGTCCATTCGACGCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 121 CGGAGTGGAGCGGGCGCTTTACCTGTACATGCTCGGTGTGGACGTGTCCATTCGACGCCA 180
9486R-1.IR_full 181 GAGAGTGGGCACCCGCCTGGTGGAGGCCACCATTGAGCTGGGTCGCCAGCGTGGATTTCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 181 GAGAGTGGGCACCCGCCTGGTGGAGGCCACCATTGAGCTGGGTCGCCAGCGTGGATTTCC 240
9486R-1.IR_full 241 CGTCGTCACATCTACCTGCAGCAACCAGAACTCCAAACGCCTTATGACCGCCCTAAACAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 241 CGTCGTCACATCTACCTGCAGCAACCAGAACTCCAAACGCCTTATGACCGCCCTAAACAT 300
9486R-1.IR_full 301 GGAGTGCATACTCACAAAGGATTACGCGGACTACAAGGACGAGCACGGGGAGATCGTCCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 301 GGAGTGCATACTCACAAAGGATTACGCGGACTACAAGGACGAGCACGGGGAGATCGTCCT 360
9486R-1.IR_full 361 GCGGGCATCCGAGCCACACACCTCCGCCAGTGTCGTGGCCATA 403
|||||||||||||||||||||||||||||||||||||||||||
9486R-1.in silico 361 GCGGGCATCCGAGCCACACACCTCCGCCAGTGTCGTGGCCATA 403
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
385 |
0 |
0 |
0 |
NM_135161.1 |
CG9486-RA (CG9486), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_170101.1 |
CG5320-RB, transcript variant B (Gdh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_206550.1 |
CG5320-RE, transcript variant E (Gdh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_079746.4 |
CG5320-RA, transcript variant A (Gdh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_206551.1 |
CG5320-RF, transcript variant F (Gdh), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_136174.1 |
CG10659-RA (CG10659), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_165299.1 |
CG10237-RA, transcript variant A (CG10237), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_136123.2 |
CG10237-RB, transcript variant B (CG10237), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_165298.1 |
CG10237-RC, transcript variant C (CG10237), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_134764.2 |
CG31935-RA (CG31935), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135211.2 |
CG31634-RA (Oatp26F), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_169845.1 |
CG31043-RA, transcript variant A (gukh), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079106.2 |
CG4817-RA (Ssrp), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079414.2 |
CG4319-RA (rpr), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_135349.2 |
CG8419-RA (CG8419), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_170420.1 |
CG18741-RA, transcript variant A (DopR2), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_079824.2 |
CG18741-RB, transcript variant B (DopR2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176247.1 |
CG33143-RB, transcript variant B (CG33143), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176246.1 |
CG33143-RC, transcript variant C (CG33143), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143019.2 |
CG6695-RA, transcript variant A (CG6695), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206565.1 |
CG6695-RB, transcript variant B (CG6695), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136296.1 |
CG5922-RA (CG5922), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168709.3 |
CG6664-RB, transcript variant B (CG6664), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168710.3 |
CG6664-RC, transcript variant C (CG6664), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140687.4 |
CG6664-RA, transcript variant A (CG6664), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141680.1 |
CG16908-RA (CG16908), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168711.2 |
CG6664-RD, transcript variant D (CG6664), mRNA |
| 0 |
0 |
0 |
8 |
11 |
NM_142363.4 |
CG5407-RB, transcript variant B (Sur-8), mRNA |
| 0 |
0 |
0 |
8 |
11 |
NM_169758.1 |
CG5407-RA, transcript variant A (Sur-8), mRNA |
| 0 |
0 |
0 |
8 |
8 |
NM_078872.4 |
CG10302-RA (bsf), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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