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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9441R-2 |
| Symbol |
Pu |
Full Name |
Punch |
| CG No |
CG9441 |
Old CG No |
CG9441 |
| Synonyms |
GTPCH, CG9441, GTPC, upi, p[Gr], l(2)57Cf, Gr, Pu |
| Accession No (Link to NCBI) |
NM_166430.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCCCAGACAAGCGAGCAAAA |
| Primer Seq. 3' |
aatctagaggtaccGCACCAGATGATGCTCGCACA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCCNCAGACA AGCGAGCAAA ATGGCAGTGG cCAAAATGGC GAGGGTGCCG CAGATGcCGT
0061 GNNCGTTGCC AcGNTCCCCA CGGGCGAAGC CTCCGCCGCC TCCGCCACGT CCGGCACCGA
0121 TCTAACGGTC TCCAAAAACA GCCAGCAGCT GAAACTGGAA ATGCTCAACT TGGAGCTGGC
0181 CAGCAATGGN AAGCGGCCAC GAGAAGTGCA CGTTCCACCA CGACCTGGAG CTGGACCACA
0241 AGCCGCCGAC GCGAGAGGCC CTCCTTCCGG ACATGGCACG CTCGTATTGT CTACTCTTGG
0301 GCGGCCTGGG CGAGAATCCC GATCGCCAGG GACTGATCAA NGACGCCGGA GCGGGCAGCC
0361 AAGGCCATGC TGTACTTCAC CAAGGGCTAC GACCAGAGTC TCGAGGATGT TCTCAATGGC
0421 GCCGTTTTCG ACGAGGATCA TGACGAAATG GTGGTGGTCA AGGATATTGA AATGTTCTCC
0481 ATGTGCGAGC ATCATCTGGT GC |
| in silico PCR Fragment |
0001 GCCCCAGACA AGCGAGCAAA ATGGCAGTGG CCAAAATGGC GAGGGTGCCG CAGATGCCGT
0061 GGCCGTTGCC ACGATCCCCA CGGGCGAAGC CTCCGCCGCC TCCGCCACGT CCGGCACCGA
0121 TCTAACGGTC TCCAAAAACA GCCAGCAGCT GAAACTGGAA ATGCTCAACT TGGAGCTGGC
0181 CAGCAATGG- AAGCGGCCAC GAGAAGTGCA CGTTCCACCA CGACCTGGAG CTGGACCACA
0241 AGCCGCCGAC GCGAGAGGCC CTCCTTCCGG ACATGGCACG CTCGTATCGT CTACTCTTGG
0301 GCGGCCTGGG CGAGAATCCC GATCGCCAGG GACTGATCAA -GACGCCGGA GCGGGCAGCC
0361 AAGGCCATGC TGTACTTCAC CAAGGGCTAC GACCAGAGTC TCGAGGATGT TCTCAATGGC
0421 GCCGTTTTCG ACGAGGATCA TGACGAAATG GTGGTGGTCA AGGATATTGA AATGTTCTCC
0481 ATGTGCGAGC ATCATCTGGT GC |
| Assemble Data |
<PRE>
9441R-2.IR_full 1 GCC<span class="snp"><tt>N</tt></span>CAGACAAGCGAGCAAAATGGCAGTGGCCAAAATGGCGAGGGTGCCGCAGATGCCGT 60
|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 1 GCC<span class="snp"><tt>C</tt></span>CAGACAAGCGAGCAAAATGGCAGTGGCCAAAATGGCGAGGGTGCCGCAGATGCCGT 60
9441R-2.IR_full 61 G<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CGTTGCCACG<span class="snp"><tt>N</tt></span>TCCCCACGGGCGAAGCCTCCGCCGCCTCCGCCACGTCCGGCACCGA 120
|<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 61 G<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>CGTTGCCACG<span class="snp"><tt>A</tt></span>TCCCCACGGGCGAAGCCTCCGCCGCCTCCGCCACGTCCGGCACCGA 120
9441R-2.IR_full 121 TCTAACGGTCTCCAAAAACAGCCAGCAGCTGAAACTGGAAATGCTCAACTTGGAGCTGGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 121 TCTAACGGTCTCCAAAAACAGCCAGCAGCTGAAACTGGAAATGCTCAACTTGGAGCTGGC 180
9441R-2.IR_full 181 CAGCAATGG<span class="snp"><tt>N</tt></span>AAGCGGCCACGAGAAGTGCACGTTCCACCACGACCTGGAGCTGGACCACA 240
|||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 181 CAGCAATGG<span class="snp"><tt>-</tt></span>AAGCGGCCACGAGAAGTGCACGTTCCACCACGACCTGGAGCTGGACCACA 240
9441R-2.IR_full 241 AGCCGCCGACGCGAGAGGCCCTCCTTCCGGACATGGCACGCTCGTAT<span class="snp"><tt>T</tt></span>GTCTACTCTTGG 300
|||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||
9441R-2.in silico 241 AGCCGCCGACGCGAGAGGCCCTCCTTCCGGACATGGCACGCTCGTAT<span class="snp"><tt>C</tt></span>GTCTACTCTTGG 300
9441R-2.IR_full 301 GCGGCCTGGGCGAGAATCCCGATCGCCAGGGACTGATCAA<span class="snp"><tt>N</tt></span>GACGCCGGAGCGGGCAGCC 360
||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
9441R-2.in silico 301 GCGGCCTGGGCGAGAATCCCGATCGCCAGGGACTGATCAA<span class="snp"><tt>-</tt></span>GACGCCGGAGCGGGCAGCC 360
9441R-2.IR_full 361 AAGGCCATGCTGTACTTCACCAAGGGCTACGACCAGAGTCTCGAGGATGTTCTCAATGGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 361 AAGGCCATGCTGTACTTCACCAAGGGCTACGACCAGAGTCTCGAGGATGTTCTCAATGGC 420
9441R-2.IR_full 421 GCCGTTTTCGACGAGGATCATGACGAAATGGTGGTGGTCAAGGATATTGAAATGTTCTCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9441R-2.in silico 421 GCCGTTTTCGACGAGGATCATGACGAAATGGTGGTGGTCAAGGATATTGAAATGTTCTCC 480
9441R-2.IR_full 481 ATGTGCGAGCATCATCTGGTGC 502
||||||||||||||||||||||
9441R-2.in silico 481 ATGTGCGAGCATCATCTGGTGC 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_166430.1 |
CG9441-RB, transcript variant B (Pu), mRNA |
| 59.95 |
289 |
1 |
1 |
3 |
NM_079077.2 |
CG9441-RC, transcript variant C (Pu), mRNA |
| 59.95 |
289 |
1 |
1 |
3 |
NM_166431.2 |
CG9441-RA, transcript variant A (Pu), mRNA |
| 0.2 |
1 |
3 |
4 |
18 |
NM_168941.2 |
CG7421-RA, transcript variant A (Nopp140), mRNA |
| 0.2 |
1 |
3 |
2 |
3 |
NM_132287.2 |
CG4040-RA (CG4040), mRNA |
| 0 |
0 |
4 |
5 |
10 |
NM_080223.2 |
CG3986-RA (Cht4), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_080139.3 |
CG8171-RA (dup), mRNA |
| 0 |
0 |
2 |
6 |
31 |
NM_079093.2 |
CG9888-RA (Fib), mRNA |
| 0 |
0 |
2 |
5 |
18 |
NM_142330.1 |
CG5225-RA (CG5225), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_164565.1 |
CG3399-RC, transcript variant C (capu), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_164566.1 |
CG3399-RD, transcript variant D (capu), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_164567.1 |
CG3399-RB, transcript variant B (capu), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_057618.2 |
CG3399-RA, transcript variant A (capu), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_168322.1 |
CG10537-RB, transcript variant B (Rdl), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_079267.2 |
CG10537-RA, transcript variant A (Rdl), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_168321.1 |
CG10537-RC, transcript variant C (Rdl), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142638.2 |
CG5412-RA (CG5412), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143705.2 |
CG12225-RA (Spt6), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143298.1 |
CG3368-RA (CG3368), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_138080.2 |
CG4612-RA (CG4612), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132507.1 |
CG2247-RA (CG2247), mRNA |
| 0 |
0 |
1 |
8 |
21 |
NM_136816.1 |
CG13214-RA, transcript variant A (CG13214), mRNA |
| 0 |
0 |
1 |
8 |
2 |
NM_143523.1 |
CG15531-RA (CG15531), mRNA |
| 0 |
0 |
1 |
7 |
13 |
NM_206095.1 |
CG13214-RB, transcript variant B (CG13214), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_137340.2 |
CG8950-RA (CG8950), mRNA |
| 0 |
0 |
1 |
6 |
16 |
NM_137671.1 |
CG15226-RA (CG15226), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_057326.4 |
CG12212-RA, transcript variant A (peb), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_001014722.1 |
CG12212-RB, transcript variant B (peb), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_166609.1 |
CG5393-RC, transcript variant C (apt), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_057831.3 |
CG5393-RB, transcript variant B (apt), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00790
:
Folate biosynthesis
dme01100
:
Metabolic pathways
dme01240
:
Biosynthesis of cofactors
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