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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9343R-2 |
| Symbol |
Trl |
Full Name |
Trithorax-like |
| CG No |
CG33261 |
Old CG No |
CG9343 |
| Synonyms |
GAF, CG9343, GAGA, trl, Trl-GAGA, E(var)3-trl, Nc70F, Adf-2-519, TfGAGA/Adf-2, CG33261, E(var)62, Gaga, unnamed, Gaf, Adf-2, Adf2, NC70F, l(3)s2325, anon-EST:fe2E12, Trl, gaga |
| Accession No (Link to NCBI) |
NM_206358.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCTCGTATCGGCAATCCAAT |
| Primer Seq. 3' |
aatctagaggtaccTTGTCCTGCTGCGTTGGTCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 gCCNCGCTAT CGGCAATCCA ATTGTTGCGC TGCCATGGCG ACCTCGTCGA TTGCACGTTG
0061 GCCGCCGGCg GGCGGAGTTT TCCCGCCCAC AAGATAGTCC TGTGCGCCGC CTCCCCCTTT
0121 CTGCTGGACT TGCTAAAGAA TACACCATGC AAGCATCCAG TGGTTATGTT GGCTGGCGTC
0181 AATGCGAACG ATCTGGAGGC GCTGCTGGAG TTTGTGTACC GCGGAGAGGT GAGCGTGGAT
0241 CACGCCCAGC TGCCGTCGCT ACTGCAGGCT GCCCAGTGCC TGAACATCCA GGGACTGGCA
0301 CCGCAGACGG TTACCAAGGA CGACTACACC ACGCACTCGA TACAACTGCA GCACATGATT
0361 CCACAACATC ACGACCAAGA CCAACTGATT GCCACGATcG NCACGGCTCC ACAGCAAACG
0421 GTTCATGCCC AGGTGGTGGA GGACATCCAT CATCAGGGCC AGATTCTCCA GGCAACGACC
0481 CAGACCaACG CAGCAGGACA A |
| in silico PCR Fragment |
0001 GCCTCG-TAT CGGCAATCCA ATTGTTGCGC TGCCATGGCG ACCTCGTCGA TTGCACGTTG
0061 GCCGCCGGCG GGCGGAGTTT TCCCGCCCAC AAGATAGTCC TGTGCGCCGC CTCCCCCTTT
0121 CTGCTGGACT TGCTAAAGAA TACACCATGC AAGCATCCAG TGGTTATGTT GGCTGGCGTC
0181 AATGCGAACG ATCTGGAGGC GCTGCTGGAG TTTGTGTACC GCGGAGAGGT GAGCGTGGAT
0241 CACGCCCAGC TGCCGTCGCT ACTGCAGGCT GCCCAGTGCC TGAACATCCA GGGACTGGCA
0301 CCGCAGACGG TTACCAAGGA CGACTACACC ACGCACTCGA TACAACTGCA GCACATGATT
0361 CCACAACATC ACGACCAAGA CCAACTGATT GCCACGATCG CCACGGCTCC ACAGCAAACG
0421 GTTCATGCCC AGGTGGTGGA GGACATCCAT CATCAGGGCC AGATTCTCCA GGCAACGACC
0481 CAGACCAACG CAGCAGGACA A |
| Assemble Data |
<PRE>
9343R-2.IR_full 1 GCC<span class="snp"><tt>N</tt></span>CG<span class="snp"><tt>C</tt></span>TATCGGCAATCCAATTGTTGCGCTGCCATGGCGACCTCGTCGATTGCACGTTG 60
|||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 1 GCC<span class="snp"><tt>T</tt></span>CG<span class="snp"><tt>-</tt></span>TATCGGCAATCCAATTGTTGCGCTGCCATGGCGACCTCGTCGATTGCACGTTG 60
9343R-2.IR_full 61 GCCGCCGGCGGGCGGAGTTTTCCCGCCCACAAGATAGTCCTGTGCGCCGCCTCCCCCTTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 61 GCCGCCGGCGGGCGGAGTTTTCCCGCCCACAAGATAGTCCTGTGCGCCGCCTCCCCCTTT 120
9343R-2.IR_full 121 CTGCTGGACTTGCTAAAGAATACACCATGCAAGCATCCAGTGGTTATGTTGGCTGGCGTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 121 CTGCTGGACTTGCTAAAGAATACACCATGCAAGCATCCAGTGGTTATGTTGGCTGGCGTC 180
9343R-2.IR_full 181 AATGCGAACGATCTGGAGGCGCTGCTGGAGTTTGTGTACCGCGGAGAGGTGAGCGTGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 181 AATGCGAACGATCTGGAGGCGCTGCTGGAGTTTGTGTACCGCGGAGAGGTGAGCGTGGAT 240
9343R-2.IR_full 241 CACGCCCAGCTGCCGTCGCTACTGCAGGCTGCCCAGTGCCTGAACATCCAGGGACTGGCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 241 CACGCCCAGCTGCCGTCGCTACTGCAGGCTGCCCAGTGCCTGAACATCCAGGGACTGGCA 300
9343R-2.IR_full 301 CCGCAGACGGTTACCAAGGACGACTACACCACGCACTCGATACAACTGCAGCACATGATT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 301 CCGCAGACGGTTACCAAGGACGACTACACCACGCACTCGATACAACTGCAGCACATGATT 360
9343R-2.IR_full 361 CCACAACATCACGACCAAGACCAACTGATTGCCACGATCG<span class="snp"><tt>N</tt></span>CACGGCTCCACAGCAAACG 420
||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
9343R-2.in silico 361 CCACAACATCACGACCAAGACCAACTGATTGCCACGATCG<span class="snp"><tt>C</tt></span>CACGGCTCCACAGCAAACG 420
9343R-2.IR_full 421 GTTCATGCCCAGGTGGTGGAGGACATCCATCATCAGGGCCAGATTCTCCAGGCAACGACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9343R-2.in silico 421 GTTCATGCCCAGGTGGTGGAGGACATCCATCATCAGGGCCAGATTCTCCAGGCAACGACC 480
9343R-2.IR_full 481 CAGACCAACGCAGCAGGACAA 501
|||||||||||||||||||||
9343R-2.in silico 481 CAGACCAACGCAGCAGGACAA 501
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_206356.2 |
CG33261-RB, transcript variant B (Trl), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_001038926.1 |
CG33261-RI, transcript variant I (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206357.2 |
CG33261-RD, transcript variant D (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206358.2 |
CG33261-RA, transcript variant A (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206360.1 |
CG33261-RF, transcript variant F (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206355.2 |
CG33261-RC, transcript variant C (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001038925.1 |
CG33261-RH, transcript variant H (Trl), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206359.2 |
CG33261-RE, transcript variant E (Trl), mRNA |
| 69.91 |
337 |
2 |
5 |
4 |
NM_001038924.1 |
CG33261-RG, transcript variant G (Trl), mRNA |
| 0.41 |
2 |
2 |
2 |
3 |
NM_140783.2 |
CG7285-RA (star1), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_131987.2 |
CG4202-RA (Sas10), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_001014589.1 |
CG33522-RA, transcript variant A (scaf6), mRNA |
| 0 |
0 |
1 |
9 |
7 |
NM_135371.3 |
CG7627-RA (CG7627), mRNA |
| 0 |
0 |
1 |
6 |
9 |
NM_141297.1 |
CG2926-RA (CG2926), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_138130.2 |
CG30420-RB, transcript variant B (Atf-2), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_166690.1 |
CG30420-RA, transcript variant A (Atf-2), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_167852.1 |
CG9097-RB, transcript variant B (bab1), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_206229.1 |
CG9097-RA, transcript variant A (bab1), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_137701.2 |
CG4030-RA (CG4030), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_169909.1 |
CG5460-RB, transcript variant B (H), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_169908.1 |
CG5460-RD, transcript variant D (H), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_169907.1 |
CG5460-RA, transcript variant A (H), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_079694.2 |
CG5460-RC, transcript variant C (H), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168416.1 |
CG32066-RB, transcript variant B (CG32066), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168417.1 |
CG32066-RC, transcript variant C (CG32066), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135186.1 |
CG16947-RA (CG16947), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_166959.1 |
CG32498-RO, transcript variant O (dnc), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_166960.1 |
CG32498-RB, transcript variant B (dnc), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166961.1 |
CG32498-RI, transcript variant I (dnc), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166965.1 |
CG32498-RJ, transcript variant J (dnc), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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