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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9324R-2

Symbol Pomp Full Name Pomp

CG No CG9324 Old CG No CG9324

Synonyms POMP, 38E.20, CG9324, Pomp

Accession No (Link to NCBI) NM_136213

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCCATCACTGAAAGTCCAGC

Primer Seq. 3'

aatctagaggtaccTCCCAGAGATTTCTCCACCAC

Predicted Fragment Size

IR fragment full Seq

0001 AGCCATCACT GAAAGTCCAG CCCGCCGAGG TGTCCGTGCT GAACGCCACC GGCCGTGTGG 
0061 GAATGCCCAC CGAGGCCAAC TGCCTGAACC AACTGGCCCA CGTCCACCGG CTGCGCGACT 
0121 CGGAGCTGAA CTACAACGAG CACCAGTACA ACCGCAACAT GCAGATGNCT GCGCAACCAC 
0181 GAGGGCCTCG GCGTGCCCCT CAAGATGGGC ATGGAGCGCT TCGCCGCACG TCAGGTGGGC 
0241 CGCCTGCCCT TCCTTTCGTC CAGCAACTTC ATGGATGATG TCCTGACTGG CCGCTGTGAT 
0301 TCCATTGGCT TCGAGGACTT CATGAACTTG CCCGAGAACA GCGAGCATAT GCGTCAGCCC 
0361 ACGCTGTGGT GGAGAAATCT CTGGGA

in silico PCR Fragment

0001 AGCCATCACT GAAAGTCCAG CCCGCCGAGG TGTCCGTGCT GAACGCCACC GGCCGTGTGG 
0061 GAATGCCCAC CGAGGCCAAC TGCCTGAACC AACTGGCCCA CGTCCACCGG CTGCGCGACT 
0121 CGGAGCTGAA CTACAACGAG CACCAGTACA ACCGCAACAT GCAGATGCTG CGCAACCACG 
0181 AGGGCCTCGG CGTGCCCCTC AAGATGGGCA TGGAGCGCTT CGCCGCACGT CAGGTGGGCC 
0241 GCCTGCCCTT CCTTTCGTCC AGCAACTTCA TGGACGATGT CCTGACTGGC CGCTGTGATT 
0301 CCATTGGCTT CGAGGACTTC ATGAACTTGC CCGAGAACAG CGAGCATATG CGTCAGCCCC 
0361 ACGCTGTGGT GGAGAAATCT CTGGGA

Assemble Data


9324R-2.IR full       1   AGCCATCACTGAAAGTCCAGCCCGCCGAGGTGTCCGTGCTGAACGCCACCGGCCGTGTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9324R-2.in silico     1   AGCCATCACTGAAAGTCCAGCCCGCCGAGGTGTCCGTGCTGAACGCCACCGGCCGTGTGG 60


9324R-2.IR full       61  GAATGCCCACCGAGGCCAACTGCCTGAACCAACTGGCCCACGTCCACCGGCTGCGCGACT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9324R-2.in silico     61  GAATGCCCACCGAGGCCAACTGCCTGAACCAACTGGCCCACGTCCACCGGCTGCGCGACT 120


9324R-2.IR full       121 CGGAGCTGAACTACAACGAGCACCAGTACAACCGCAACATGCAGATGNCTGCGCAACCAC 180
                          |||||||||||||||||||||||||||||||||||||||||||||||  |||||||||||
9324R-2.in silico     121 CGGAGCTGAACTACAACGAGCACCAGTACAACCGCAACATGCAGATGC-TGCGCAACCAC 180


9324R-2.IR full       181 GAGGGCCTCGGCGTGCCCCTCAAGATGGGCATGGAGCGCTTCGCCGCACGTCAGGTGGGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9324R-2.in silico     181 GAGGGCCTCGGCGTGCCCCTCAAGATGGGCATGGAGCGCTTCGCCGCACGTCAGGTGGGC 240


9324R-2.IR full       241 CGCCTGCCCTTCCTTTCGTCCAGCAACTTCATGGATGATGTCCTGACTGGCCGCTGTGAT 300
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
9324R-2.in silico     241 CGCCTGCCCTTCCTTTCGTCCAGCAACTTCATGGACGATGTCCTGACTGGCCGCTGTGAT 300


9324R-2.IR full       301 TCCATTGGCTTCGAGGACTTCATGAACTTGCCCGAGAACAGCGAGCATATGCGTCAGCCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9324R-2.in silico     301 TCCATTGGCTTCGAGGACTTCATGAACTTGCCCGAGAACAGCGAGCATATGCGTCAGCCC 360


9324R-2.IR full       361 -ACGCTGTGGTGGAGAAATCTCTGGGA 387
                           ||||||||||||||||||||||||||
9324R-2.in silico     361 CACGCTGTGGTGGAGAAATCTCTGGGA 387

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	368	0	0	0	NM_136213.2	Pomp CG9324-RA (Pomp), mRNA
0.27	1	2	4	3	NM_141876.1	CG12213-RB, transcript variant B (CG12213), mRNA
0.27	1	2	4	3	NM_169442.1	CG12213-RA, transcript variant A (CG12213), mRNA
0	0	3	4	4	NM_058133.3	ligatin CG31426-RA (ligatin), mRNA
0	0	2	3	3	NM_136748.2	CG17765-RA (CG17765), mRNA
0	0	1	5	5	NM_167687.1	CG11943-RB, transcript variant B (CG11943), mRNA
0	0	1	5	5	NM_134518.2	CG11943-RA, transcript variant A (CG11943), mRNA
0	0	1	5	2	NM_136869.2	CG13183-RA (CG13183), mRNA
0	0	1	4	9	NM_135589.2	CG17104-RA (CG17104), mRNA
0	0	1	4	4	NM_176316.1	Mocs1 CG33048-RC, transcript variant C (Mocs1), mRNA
0	0	1	4	4	NM_176229.1	CG33147-RA (CG33147), mRNA
0	0	1	3	10	NM_138033.1	CG13563-RA (CG13563), mRNA
0	0	1	3	7	NM_078815.2	Leucine-rich repeat 47 CG6098-RA (Lrr47), mRNA
0	0	1	3	6	NM_057943.3	miranda CG12249-RA, transcript variant A (mira), mRNA
0	0	1	3	6	NM_057944.3	miranda CG12249-RB, transcript variant B (mira), mRNA
0	0	1	2	6	NM_133090.1	CG6696-RA (CG6696), mRNA
0	0	1	2	3	NM_079730.2	klingon CG6669-RA (klg), mRNA
0	0	1	2	3	NM_132294.2	Anaphase Promoting Complex 4 CG32707-RA (APC4), mRNA
0	0	1	2	2	NM_136455.1	CG2093-RA (CG2093), mRNA
0	0	0	7	3	NM_137944.2	yellow-d2 CG9891-RA (yellow-d2), mRNA
0	0	0	6	5	NM_057842.2	viking CG16858-RA (vkg), mRNA
0	0	0	5	5	NM_080166.2	scattered CG3766-RA (scat), mRNA
0	0	0	5	4	NM_139425.1	CG5691-RA (CG5691), mRNA
0	0	0	4	8	NM_001042796.1	rugose CG6775-RC, transcript variant C (rg), mRNA
0	0	0	4	8	NM_080023.1	rugose CG6775-RA, transcript variant A (rg), mRNA
0	0	0	4	8	NM_167028.1	rugose CG6775-RB, transcript variant B (rg), mRNA
0	0	0	4	5	NM_170524.1	warts CG12072-RA (wts), mRNA
0	0	0	4	5	NM_169228.1	CG31258-RA (CG31258), mRNA
0	0	0	4	4	NM_170415.2	CG31041-RA (CG31041), mRNA
0	0	0	4	4	NM_167371.1	CG32635-RA (CG32635), mRNA

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