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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9323R-4

Symbol CG9323 Full Name CG9323

CG No CG9323 Old CG No CG9323

Synonyms 38E.15, cg9323, CG9323

Accession No (Link to NCBI) NM_136212.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCCAAGCTGGACGAGAGACT

Primer Seq. 3'

aatctagaggtaccGGAGATCTGTTTCCACGCTGC

Predicted Fragment Size

IR fragment full Seq

0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CgNCAGTTGG AGGAGAATGC 
0061 CAAGAAACGG CTAGCAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT 
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC 
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG 
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG 
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG 
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT 
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG 
0481 CAGCGTGGAA ACAGATCTCC

in silico PCR Fragment

0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CGCCAGTTGG AGGAGAATGC 
0061 CAAGAAACGG CTAGAAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT 
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC 
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG 
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG 
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG 
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT 
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG 
0481 CAGCGTGGAA ACAGATCTCC

Assemble Data


9323R-4.IR_full       1   CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGNCAGTTGGAGGAGAATGC 60
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
9323R-4.in silico     1   CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGCCAGTTGGAGGAGAATGC 60


9323R-4.IR_full       61  CAAGAAACGGCTAGCAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     61  CAAGAAACGGCTAGAAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120


9323R-4.IR_full       121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180


9323R-4.IR_full       181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240


9323R-4.IR_full       241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300


9323R-4.IR_full       301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360


9323R-4.IR_full       361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420


9323R-4.IR_full       421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-4.in silico     421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480


9323R-4.IR_full       481 CAGCGTGGAAACAGATCTCC 500
                          ||||||||||||||||||||
9323R-4.in silico     481 CAGCGTGGAAACAGATCTCC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136212.1	CG9323-RA (CG9323), mRNA
0	0	10	6	4	NM_057393.3	CG3158-RA (spn-E), mRNA
0	0	3	4	2	NM_168751.1	CG32188-RA (CG32188), mRNA
0	0	3	4	2	NM_168752.1	CG32189-RA (CG32189), mRNA
0	0	3	2	2	NM_144343.2	CG8250-RA (Alk), mRNA
0	0	2	5	3	NM_143371.1	CG9990-RA, transcript variant A (CG9990), mRNA
0	0	2	5	3	NM_170376.1	CG9990-RB, transcript variant B (CG9990), mRNA
0	0	2	4	4	NM_143997.2	CG14545-RA (CG14545), mRNA
0	0	2	4	2	NM_166367.1	CG30130-RA (CG30130), mRNA
0	0	2	3	9	NM_001031986.1	CG33719-RB, transcript variant B (Pif1A), mRNA
0	0	2	3	8	NM_134551.4	CG32506-RA (CG32506), mRNA
0	0	2	3	8	NM_001031985.1	CG33720-RA, transcript variant A (Pif1B), mRNA
0	0	2	3	8	NM_001031987.1	CG33719-RA, transcript variant A (Pif1A), mRNA
0	0	2	3	5	NM_079106.2	CG4817-RA (Ssrp), mRNA
0	0	2	3	5	NM_164411.2	CG4896-RB, transcript variant B (CG4896), mRNA
0	0	2	3	5	NM_134739.5	CG4896-RD, transcript variant D (CG4896), mRNA
0	0	2	3	5	NM_164409.2	CG4896-RC, transcript variant C (CG4896), mRNA
0	0	2	3	5	NM_164410.2	CG4896-RA, transcript variant A (CG4896), mRNA
0	0	2	3	4	NM_138003.2	CG5591-RA (CG5591), mRNA
0	0	1	5	9	NM_166844.1	CG17896-RA, transcript variant A (CG17896), mRNA
0	0	1	5	9	NM_130489.2	CG17896-RB, transcript variant B (CG17896), mRNA
0	0	1	4	2	NM_136775.2	CG12344-RA (CG12344), mRNA
0	0	1	4	2	NM_131948.1	CG3081-RA (CG3081), mRNA
0	0	1	3	3	NM_135786.1	CG6108-RA (CG6108), mRNA
0	0	1	3	2	NM_143157.1	CG5039-RA (CG5039), mRNA
0	0	1	3	2	NM_167233.1	CG32687-RA (CG32687), mRNA
0	0	1	3	2	NM_140972.2	CG5932-RA (CG5932), mRNA
0	0	1	2	10	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	1	2	8	NM_166126.1	CG18255-RC, transcript variant C (Strn-Mlck), mRNA
0	0	1	2	8	NM_166127.1	CG18255-RF, transcript variant F (Strn-Mlck), mRNA

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