National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9323R-2 
 Symbol CG9323  Full Name CG9323 
 CG No CG9323  Old CG No CG9323 
 Synonyms 38E.15, cg9323, CG9323 
 Accession No (Link to NCBI) NM_136212.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCCCCAAGCTGGACGAGAGACT 
 Primer Seq. 3'
aatctagaggtaccGGAGATCTGTTTCCACGCTGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CgNCAGTTGG AGGAGAATGC 
0061 CAAGAAACGG CTAGCAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT 
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC 
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG 
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG 
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG 
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT 
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG 
0481 CAGCGTGGAA ACAGATCTCC  
 in silico PCR Fragment
0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CGCCAGTTGG AGGAGAATGC 
0061 CAAGAAACGG CTAGAAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT 
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC 
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG 
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG 
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG 
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT 
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG 
0481 CAGCGTGGAA ACAGATCTCC  
 Assemble Data

9323R-2.IR_full       1   CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGNCAGTTGGAGGAGAATGC 60
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
9323R-2.in silico     1   CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGCCAGTTGGAGGAGAATGC 60


9323R-2.IR_full       61  CAAGAAACGGCTAGCAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     61  CAAGAAACGGCTAGAAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120


9323R-2.IR_full       121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180


9323R-2.IR_full       181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240


9323R-2.IR_full       241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300


9323R-2.IR_full       301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360


9323R-2.IR_full       361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420


9323R-2.IR_full       421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9323R-2.in silico     421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480


9323R-2.IR_full       481 CAGCGTGGAAACAGATCTCC 500
                          ||||||||||||||||||||
9323R-2.in silico     481 CAGCGTGGAAACAGATCTCC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136212.1  CG9323-RA (CG9323), mRNA 
0   10  NM_057393.3  CG3158-RA (spn-E), mRNA 
0   NM_168751.1  CG32188-RA (CG32188), mRNA 
0   NM_168752.1  CG32189-RA (CG32189), mRNA 
0   NM_144343.2  CG8250-RA (Alk), mRNA 
0   NM_143371.1  CG9990-RA, transcript variant A (CG9990), mRNA 
0   NM_170376.1  CG9990-RB, transcript variant B (CG9990), mRNA 
0   NM_143997.2  CG14545-RA (CG14545), mRNA 
0   NM_166367.1  CG30130-RA (CG30130), mRNA 
0   NM_001031986.1  CG33719-RB, transcript variant B (Pif1A), mRNA 
0   NM_134551.4  CG32506-RA (CG32506), mRNA 
0   NM_001031985.1  CG33720-RA, transcript variant A (Pif1B), mRNA 
0   NM_001031987.1  CG33719-RA, transcript variant A (Pif1A), mRNA 
0   NM_079106.2  CG4817-RA (Ssrp), mRNA 
0   NM_164411.2  CG4896-RB, transcript variant B (CG4896), mRNA 
0   NM_134739.5  CG4896-RD, transcript variant D (CG4896), mRNA 
0   NM_164409.2  CG4896-RC, transcript variant C (CG4896), mRNA 
0   NM_164410.2  CG4896-RA, transcript variant A (CG4896), mRNA 
0   NM_138003.2  CG5591-RA (CG5591), mRNA 
0   NM_166844.1  CG17896-RA, transcript variant A (CG17896), mRNA 
0   NM_130489.2  CG17896-RB, transcript variant B (CG17896), mRNA 
0   NM_136775.2  CG12344-RA (CG12344), mRNA 
0   NM_131948.1  CG3081-RA (CG3081), mRNA 
0   NM_135786.1  CG6108-RA (CG6108), mRNA 
0   NM_143157.1  CG5039-RA (CG5039), mRNA 
0   NM_167233.1  CG32687-RA (CG32687), mRNA 
0   NM_140972.2  CG5932-RA (CG5932), mRNA 
0   10  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   NM_166126.1  CG18255-RC, transcript variant C (Strn-Mlck), mRNA 
0   NM_166127.1  CG18255-RF, transcript variant F (Strn-Mlck), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
++ 
DC
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.