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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9323R-2 |
| Symbol |
CG9323 |
Full Name |
CG9323 |
| CG No |
CG9323 |
Old CG No |
CG9323 |
| Synonyms |
38E.15, cg9323, CG9323 |
| Accession No (Link to NCBI) |
NM_136212.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCCAAGCTGGACGAGAGACT |
| Primer Seq. 3' |
aatctagaggtaccGGAGATCTGTTTCCACGCTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CgNCAGTTGG AGGAGAATGC
0061 CAAGAAACGG CTAGCAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG
0481 CAGCGTGGAA ACAGATCTCC |
| in silico PCR Fragment |
0001 CCCCAAGCTG GACGAGAGAC TGCAGCTGGA ACTGGGGCAA CGCCAGTTGG AGGAGAATGC
0061 CAAGAAACGG CTAGAAGCAC GGAAAAAACT GCCCACCATG AAATACGCCG ACGATATTAT
0121 CCAGGCTGTG CGGGAAAACC AAGTAATACT CATTGTAGGA AGCACTGGAT GTGGCAAGAC
0181 CACTCAGGTG CCTCAAATCC TCCTCGACGA CGCCATTTCC CGCGGATGTG CCTCGTCGTG
0241 CCGCATCATT TGCACTCAGC CACGAAGGAT TTCAGCCATC GCCATTGCCG AGTGGGTGAG
0301 TTACGAGCGC TGCGAGAGCC TGGGCAATTC GGTAGGCTAC CAGATTCGCC TAGAGAGCAG
0361 GAAGGCCAGG GAGAGAGCCT CCATCACTTA CTGCACCACA GGCGTTCTAC TGCAGCAGCT
0421 TCAATCGGAC CCACTGATGC ACAATCTAAG TGTCTTAATC CTGGATGAGA TCCACGAACG
0481 CAGCGTGGAA ACAGATCTCC |
| Assemble Data |
9323R-2.IR_full 1 CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGNCAGTTGGAGGAGAATGC 60
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9323R-2.in silico 1 CCCCAAGCTGGACGAGAGACTGCAGCTGGAACTGGGGCAACGCCAGTTGGAGGAGAATGC 60
9323R-2.IR_full 61 CAAGAAACGGCTAGCAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120
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9323R-2.in silico 61 CAAGAAACGGCTAGAAGCACGGAAAAAACTGCCCACCATGAAATACGCCGACGATATTAT 120
9323R-2.IR_full 121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180
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9323R-2.in silico 121 CCAGGCTGTGCGGGAAAACCAAGTAATACTCATTGTAGGAAGCACTGGATGTGGCAAGAC 180
9323R-2.IR_full 181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240
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9323R-2.in silico 181 CACTCAGGTGCCTCAAATCCTCCTCGACGACGCCATTTCCCGCGGATGTGCCTCGTCGTG 240
9323R-2.IR_full 241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300
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9323R-2.in silico 241 CCGCATCATTTGCACTCAGCCACGAAGGATTTCAGCCATCGCCATTGCCGAGTGGGTGAG 300
9323R-2.IR_full 301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360
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9323R-2.in silico 301 TTACGAGCGCTGCGAGAGCCTGGGCAATTCGGTAGGCTACCAGATTCGCCTAGAGAGCAG 360
9323R-2.IR_full 361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420
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9323R-2.in silico 361 GAAGGCCAGGGAGAGAGCCTCCATCACTTACTGCACCACAGGCGTTCTACTGCAGCAGCT 420
9323R-2.IR_full 421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480
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9323R-2.in silico 421 TCAATCGGACCCACTGATGCACAATCTAAGTGTCTTAATCCTGGATGAGATCCACGAACG 480
9323R-2.IR_full 481 CAGCGTGGAAACAGATCTCC 500
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9323R-2.in silico 481 CAGCGTGGAAACAGATCTCC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_136212.1 |
CG9323-RA (CG9323), mRNA |
| 0 |
0 |
10 |
6 |
4 |
NM_057393.3 |
CG3158-RA (spn-E), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168751.1 |
CG32188-RA (CG32188), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168752.1 |
CG32189-RA (CG32189), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_144343.2 |
CG8250-RA (Alk), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_143371.1 |
CG9990-RA, transcript variant A (CG9990), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_170376.1 |
CG9990-RB, transcript variant B (CG9990), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_143997.2 |
CG14545-RA (CG14545), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_166367.1 |
CG30130-RA (CG30130), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_001031986.1 |
CG33719-RB, transcript variant B (Pif1A), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_134551.4 |
CG32506-RA (CG32506), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_001031985.1 |
CG33720-RA, transcript variant A (Pif1B), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_001031987.1 |
CG33719-RA, transcript variant A (Pif1A), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_079106.2 |
CG4817-RA (Ssrp), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_164411.2 |
CG4896-RB, transcript variant B (CG4896), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_134739.5 |
CG4896-RD, transcript variant D (CG4896), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_164409.2 |
CG4896-RC, transcript variant C (CG4896), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_164410.2 |
CG4896-RA, transcript variant A (CG4896), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_138003.2 |
CG5591-RA (CG5591), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_166844.1 |
CG17896-RA, transcript variant A (CG17896), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_130489.2 |
CG17896-RB, transcript variant B (CG17896), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_136775.2 |
CG12344-RA (CG12344), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_131948.1 |
CG3081-RA (CG3081), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135786.1 |
CG6108-RA (CG6108), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143157.1 |
CG5039-RA (CG5039), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167233.1 |
CG32687-RA (CG32687), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140972.2 |
CG5932-RA (CG5932), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_166127.1 |
CG18255-RF, transcript variant F (Strn-Mlck), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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