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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9320R-2

Symbol

CG9320

Full Name

CG9320

CG No

CG9320

Old CG No

CG9320

Synonyms

38E.14, CG9320

Accession No (Link to NCBI)

NM_136211.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGTGGCCTTTAGCGGCAAGAA

Primer Seq. 3'

aatctagaggtaccAGAACTCCAGGACACGCCATA

Predicted Fragment Size

IR fragment full Seq

0001 GGTGGCCTTT AGCGGCAAGA AGAAGAAGGA CCAGATGTTG CAAAAgCGCA ACACAAAAGG 
0061 TCCCCCAAAG TATCTGAGGA GCACGCAGGA GTCGTATGAG GACAGCGATG TGCCGGAAAC 
0121 GACCAGGAAA CTCATGGAGC AGCCTTTTGC GCGCGGCGGA AACCGAAACA AGAACGTCAA 
0181 TCGCTATAAT TTGCAATTCT ACCAGGAGGG CAAAAAGGAA CTGGAGCAAA TGAAACAAGA 
0241 GGGCTTTAAA CCGTTCGAGA AACTCAGTCC CGCTCAGCGG GAAGTGGACG ACCGATACTT 
0301 TGCCGGCTGC GACTTCCCAG TTCGTCCACC TTGGACGCTA ACGGAGTCCA AGGAAGAGCT 
0361 TGATCGCACC GAGAATCGCT ACTTTAAGGA ATACGTGGAC GAGCTGCAGA AAAAGCAGCG 
0421 TGCTGGAGAT TCGAAGGAGC TTTCGCTGTT TGAGCTGAAT CTGGAAACCT GGCGTCAGCT 
0481 ATGGCGTGTC CTGGAGTTCT

in silico PCR Fragment

0001 GGTGGCCTTT AGCGGCAAGA AGAAGAAGGA CCAGATGTTG CAAAAGCGCA ACACAAAAGG 
0061 TCCCCCAAAG TATCTGAGGA GCACGCAGGA GTCGTATGAG GACAGCGATG TGCCGGAAAC 
0121 GACCAGGAAA CTCATGGAGC AGCCTTTTGC GCGCGGCGGA AACCGAAACA AGAACGTCAA 
0181 TCGCTATAAT TTGCAATTCT ACCAGGAGGG CAAAAAGGAA CTGGAGCAAA TGAAACAAGA 
0241 GGGCTTTAAA CCGTTCGAGA AACTCAGTCC CGCTCAGCGG GAAGTGGACG ACCGATACTT 
0301 TGCCGGCTGC GACTTCCCAG TTCGTCCACC TTGGACGCTA ACGGAGTCCA AGGAAGAGCT 
0361 TGATCGCACC GAGAATCGCT ACTTTAAGGA ATACGTGGAC GAGCTGCAGA AAAAGCAGCG 
0421 TGCTGGAGAT TCGAAGGAGC TTTCGCTGTT TGAGCTGAAT CTGGAAACCT GGCGTCAGCT 
0481 ATGGCGTGTC CTGGAGTTCT

Assemble Data


9320R-2.IR_full       1   GGTGGCCTTTAGCGGCAAGAAGAAGAAGGACCAGATGTTGCAAAAGCGCAACACAAAAGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     1   GGTGGCCTTTAGCGGCAAGAAGAAGAAGGACCAGATGTTGCAAAAGCGCAACACAAAAGG 60


9320R-2.IR_full       61  TCCCCCAAAGTATCTGAGGAGCACGCAGGAGTCGTATGAGGACAGCGATGTGCCGGAAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     61  TCCCCCAAAGTATCTGAGGAGCACGCAGGAGTCGTATGAGGACAGCGATGTGCCGGAAAC 120


9320R-2.IR_full       121 GACCAGGAAACTCATGGAGCAGCCTTTTGCGCGCGGCGGAAACCGAAACAAGAACGTCAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     121 GACCAGGAAACTCATGGAGCAGCCTTTTGCGCGCGGCGGAAACCGAAACAAGAACGTCAA 180


9320R-2.IR_full       181 TCGCTATAATTTGCAATTCTACCAGGAGGGCAAAAAGGAACTGGAGCAAATGAAACAAGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     181 TCGCTATAATTTGCAATTCTACCAGGAGGGCAAAAAGGAACTGGAGCAAATGAAACAAGA 240


9320R-2.IR_full       241 GGGCTTTAAACCGTTCGAGAAACTCAGTCCCGCTCAGCGGGAAGTGGACGACCGATACTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     241 GGGCTTTAAACCGTTCGAGAAACTCAGTCCCGCTCAGCGGGAAGTGGACGACCGATACTT 300


9320R-2.IR_full       301 TGCCGGCTGCGACTTCCCAGTTCGTCCACCTTGGACGCTAACGGAGTCCAAGGAAGAGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     301 TGCCGGCTGCGACTTCCCAGTTCGTCCACCTTGGACGCTAACGGAGTCCAAGGAAGAGCT 360


9320R-2.IR_full       361 TGATCGCACCGAGAATCGCTACTTTAAGGAATACGTGGACGAGCTGCAGAAAAAGCAGCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     361 TGATCGCACCGAGAATCGCTACTTTAAGGAATACGTGGACGAGCTGCAGAAAAAGCAGCG 420


9320R-2.IR_full       421 TGCTGGAGATTCGAAGGAGCTTTCGCTGTTTGAGCTGAATCTGGAAACCTGGCGTCAGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9320R-2.in silico     421 TGCTGGAGATTCGAAGGAGCTTTCGCTGTTTGAGCTGAATCTGGAAACCTGGCGTCAGCT 480


9320R-2.IR_full       481 ATGGCGTGTCCTGGAGTTCT 500
                          ||||||||||||||||||||
9320R-2.in silico     481 ATGGCGTGTCCTGGAGTTCT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136211.3	CG9320-RA (CG9320), mRNA
0	0	4	6	2	NM_168144.1	CG32409-RA (CG32409), mRNA
0	0	3	3	4	NM_135873.2	CG12636-RA (CG12636), mRNA
0	0	2	4	2	NM_079000.2	CG3879-RA (Mdr49), mRNA
0	0	2	3	6	NM_001031980.1	CG9638-RB, transcript variant B (Ada2b), mRNA
0	0	2	3	6	NM_141516.2	CG9638-RA, transcript variant A (Ada2b), mRNA
0	0	2	3	2	NM_136651.1	CG1888-RA (CG1888), mRNA
0	0	2	3	2	NM_001038869.1	CG33958-RA (CG33958), mRNA
0	0	2	2	4	NM_176436.1	CG8176-RB, transcript variant B (CG8176), mRNA
0	0	2	2	4	NM_176435.1	CG8176-RA, transcript variant A (CG8176), mRNA
0	0	1	7	14	NM_169883.1	CG4836-RA, transcript variant A (CG4836), mRNA
0	0	1	7	14	NM_142599.1	CG4836-RC, transcript variant C (CG4836), mRNA
0	0	1	7	13	NM_169882.1	CG4836-RB, transcript variant B (CG4836), mRNA
0	0	1	7	9	NM_143300.1	CG3339-RA, transcript variant A (CG3339), mRNA
0	0	1	7	9	NM_001043297.1	CG3339-RB, transcript variant B (CG3339), mRNA
0	0	1	4	9	NM_078924.3	CG12165-RA (Incenp), mRNA
0	0	1	4	2	NM_143522.2	CG9747-RA (CG9747), mRNA
0	0	1	4	2	NM_170184.1	CG6238-RB, transcript variant B (ssh), mRNA
0	0	1	4	2	NM_079768.2	CG6238-RA, transcript variant A (ssh), mRNA
0	0	1	3	5	NM_139541.1	CG12017-RA (CG12017), mRNA
0	0	1	3	2	NM_142269.1	CG8925-RA (CG8925), mRNA
0	0	1	2	4	NM_057837.3	CG1616-RA (dpa), mRNA
0	0	1	2	3	NM_169090.1	CG2182-RB, transcript variant B (CG2182), mRNA
0	0	1	2	3	NM_141313.1	CG2182-RA, transcript variant A (CG2182), mRNA
0	0	1	2	3	NM_137134.2	CG12861-RA (CG12861), mRNA
0	0	0	14	49	NM_137093.4	CG30069-RA (CG30069), mRNA
0	0	0	6	8	NM_144347.2	CG12149-RA (c12.2), mRNA
0	0	0	5	6	NM_078776.4	CG31629-RA, transcript variant A (Pvf3), mRNA
0	0	0	5	3	NM_143132.2	CG11902-RA (CG11902), mRNA
0	0	0	4	10	NM_137150.2	CG10209-RA (CG10209), mRNA

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