NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9238R-3

Symbol

CG9238

Full Name

CG9238

CG No

CG9238

Old CG No

CG9238

Synonyms

anon-WO0118547.400, CG9238

Accession No (Link to NCBI)

NM_168589.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCAGTCGTTCCGGACGGAGTT

Primer Seq. 3'

aatctagaggtaccGGTGCCCACGACAATCGATTC

Predicted Fragment Size

IR fragment full Seq

0001 GCAGTCGTTC CGGACGGAGT TACCTGGAGC GAATGGCCTC GNNGCCGCTA CTCACCAATC 
0061 AGCCCAAATC CTGTCTCAGC CGCAAATCCT GCCTGCACAA ATCCCAGAAC ACTACAGCCA 
0121 GTTCCGCAGA TCTCTCGCCG ACGGACACCG ATGTCGCGGA CAGTGCCAGT CATAGCTGTA 
0181 TCTGCAGCAA GGATAGTGAC AGCAACGGCG AAGTGAGCCG TGGCCNCCAA AAAGCATGTG 
0241 ATCTTCGCTG ATGATGAGGG TCTATCGCTA ACCGAGGTTC GTGTGATGTC CGAACCCTCG 
0301 AATGTGCCGC CATATTGGAG CATGAAGTTT CTGGAGCAAA TTACACAGGG CCTGGTCAGC 
0361 CCACATCCTC CGGATCAGTG GACAGTGGAC TTCAAGCAGC CAGCCTCAGA CTATCTATCA 
0421 TTTAGACAAA AGATAGAACG CGACTTTGTT TCCTTGGAGA ATGTGATCGT CAAGGATGAG 
0481 GaATCGATTG TCGTGGGCAC C

in silico PCR Fragment

0001 GCAGTCGTTC CGGACGGAGT TACCTGGAGC GAATGGCCTC GGCGCCGCTA CTCACCAATC 
0061 AGCCCAAATC CTGTCTCAGC CGCAAATCCT GCCTGCACAA ATCCCAGAAC ACTACAGCCA 
0121 GTTCCGCAGA TCTCTCGCCG ACGGACACCG ATGTCGCGGA CAGTGCCAGT CATAGCTGTA 
0181 TCTGCAGCAA GGATAGTGAC AGCAACGGCG AAGTGAGCCG TGGCC-CCAA AAAGCATGTG 
0241 ATCTTCGCTG ATGATGAGGG TCTATCGCTA ACCGAGGTTC GTGTGATGTC CGAACCCTCG 
0301 AATGTGCCGC CATATTGGAG CATGAAGTTT CTGGAGCAAA TTACACAGGG CCTGGTCAGC 
0361 CCACATCCTC CGGATCAGTG GACAGTGGAC TTCAAGCAGC CAGCCTCAGA CTATCTATCA 
0421 TTTAGACAAA AGATAGAACG CGACTTTGTT TCCTTGGAGA ATGTGATCGT CAAGGATGAG 
0481 GAATCGATTG TCGTGGGCAC C

Assemble Data

<PRE>

9238R-3.IR_full       1   GCAGTCGTTCCGGACGGAGTTACCTGGAGCGAATGGCCTCG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GCCGCTACTCACCAATC 60
                          |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
9238R-3.in silico     1   GCAGTCGTTCCGGACGGAGTTACCTGGAGCGAATGGCCTCG<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>GCCGCTACTCACCAATC 60


9238R-3.IR_full       61  AGCCCAAATCCTGTCTCAGCCGCAAATCCTGCCTGCACAAATCCCAGAACACTACAGCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     61  AGCCCAAATCCTGTCTCAGCCGCAAATCCTGCCTGCACAAATCCCAGAACACTACAGCCA 120


9238R-3.IR_full       121 GTTCCGCAGATCTCTCGCCGACGGACACCGATGTCGCGGACAGTGCCAGTCATAGCTGTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     121 GTTCCGCAGATCTCTCGCCGACGGACACCGATGTCGCGGACAGTGCCAGTCATAGCTGTA 180


9238R-3.IR_full       181 TCTGCAGCAAGGATAGTGACAGCAACGGCGAAGTGAGCCGTGGCC<span class="snp"><tt>N</tt></span>CCAAAAAGCATGTG 240
                          |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||
9238R-3.in silico     181 TCTGCAGCAAGGATAGTGACAGCAACGGCGAAGTGAGCCGTGGCC<span class="snp"><tt>-</tt></span>CCAAAAAGCATGTG 240


9238R-3.IR_full       241 ATCTTCGCTGATGATGAGGGTCTATCGCTAACCGAGGTTCGTGTGATGTCCGAACCCTCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     241 ATCTTCGCTGATGATGAGGGTCTATCGCTAACCGAGGTTCGTGTGATGTCCGAACCCTCG 300


9238R-3.IR_full       301 AATGTGCCGCCATATTGGAGCATGAAGTTTCTGGAGCAAATTACACAGGGCCTGGTCAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     301 AATGTGCCGCCATATTGGAGCATGAAGTTTCTGGAGCAAATTACACAGGGCCTGGTCAGC 360


9238R-3.IR_full       361 CCACATCCTCCGGATCAGTGGACAGTGGACTTCAAGCAGCCAGCCTCAGACTATCTATCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     361 CCACATCCTCCGGATCAGTGGACAGTGGACTTCAAGCAGCCAGCCTCAGACTATCTATCA 420


9238R-3.IR_full       421 TTTAGACAAAAGATAGAACGCGACTTTGTTTCCTTGGAGAATGTGATCGTCAAGGATGAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9238R-3.in silico     421 TTTAGACAAAAGATAGAACGCGACTTTGTTTCCTTGGAGAATGTGATCGTCAAGGATGAG 480


9238R-3.IR_full       481 GAATCGATTGTCGTGGGCACC 501
                          |||||||||||||||||||||
9238R-3.in silico     481 GAATCGATTGTCGTGGGCACC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168589.1	CG9238-RA, transcript variant A (CG9238), mRNA
100	482	0	0	0	NM_140451.2	CG9238-RB, transcript variant B (CG9238), mRNA
0	0	3	2	2	NM_137351.2	CG11395-RA (CG11395), mRNA
0	0	2	3	2	NM_168626.1	CG7439-RC, transcript variant C (AGO2), mRNA
0	0	2	3	2	NM_140518.1	CG7439-RB, transcript variant B (AGO2), mRNA
0	0	1	5	2	NM_135772.1	CG9426-RA (CG9426), mRNA
0	0	1	4	4	NM_168111.3	CG32423-RA, transcript variant A (alan-shepard), mRNA
0	0	1	4	3	NM_206428.1	CG33293-RA (CG33293), mRNA
0	0	1	4	3	NM_168114.2	CG32423-RC, transcript variant C (alan-shepard), mRNA
0	0	1	4	3	NM_168112.1	CG32423-RB, transcript variant B (alan-shepard), mRNA
0	0	1	4	3	NM_168113.2	CG32423-RD, transcript variant D (alan-shepard), mRNA
0	0	1	3	3	NM_143574.1	CG15546-RA (CG15546), mRNA
0	0	1	3	2	NM_165124.2	CG3938-RD, transcript variant D (CycE), mRNA
0	0	1	3	2	NM_165125.2	CG3938-RE, transcript variant E (CycE), mRNA
0	0	1	3	2	NM_057611.4	CG3938-RA, transcript variant A (CycE), mRNA
0	0	1	3	2	NM_165123.2	CG3938-RC, transcript variant C (CycE), mRNA
0	0	1	2	7	NM_132310.2	CG2194-RB, transcript variant B (Reg-3), mRNA
0	0	1	2	7	NM_167182.1	CG2194-RC, transcript variant C (Reg-3), mRNA
0	0	1	2	6	NM_169676.1	CG31150-RA (CG31150), mRNA
0	0	1	2	3	NM_141337.1	CG2031-RA (Hpr1), mRNA
0	0	1	2	2	NM_139564.1	CG10357-RA (CG10357), mRNA
0	0	1	2	2	NM_176436.1	CG8176-RB, transcript variant B (CG8176), mRNA
0	0	1	2	2	NM_176435.1	CG8176-RA, transcript variant A (CG8176), mRNA
0	0	0	5	5	NM_134899.2	CG17259-RA (CG17259), mRNA
0	0	0	5	2	NM_079329.2	CG11276-RB, transcript variant B (RpS4), mRNA
0	0	0	5	2	NM_168537.1	CG11276-RA, transcript variant A (RpS4), mRNA
0	0	0	4	6	NM_080200.2	CG5818-RA (mRpL4), mRNA
0	0	0	4	4	NM_079439.2	CG9383-RA (asf1), mRNA
0	0	0	4	3	NM_132158.1	CG1958-RA (CG1958), mRNA
0	0	0	4	3	NM_141354.2	CG10287-RA (Gasp), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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