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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9226R-1 
 Symbol CG9226  Full Name CG9226 
 CG No CG9226  Old CG No CG9226 
 Synonyms CG9226 
 Accession No (Link to NCBI) NM_135153.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgATGCTGAATCACAACGTGAGC 
 Primer Seq. 3'
aatctagaggtaccGCTGCACCGTATCTGCGGAGT 
 Predicted Fragment Size
499 
 IR fragment full Seq
0001 ATGCTGAATC ACAACGTGAG CTTCAGCAGC ACCATGGTCA CGTCCAATGG CGATCTTTCG 
0061 TTGCCCAAAC TGAGCGTGAC TCTTTCGGCG GAGGAGATCC TAAAGCTGCG TTCCGGGCGT 
0121 CCAAAGAACG CCGTTGAATC GCCGCATGCG GGTGTGCCCA TGGAGACAAG CCTGGCTGCC 
0181 GAGGAGGAAG CGAATGGGGA TGAGGAAGAG GAAAGCGTGC GGGTCGTCGA CATAGAGGAC 
0241 ACTTTGGAGC TGACGTCGAA TGGCAATGAG GCGAAGCCGG AAGACCAGGA GCTTGCGATT 
0301 GCTCCCGTCC TCCAGTACTT CCAGGGAGCA CTTGTTGAGT TTGGGCCGAC GCTGCTGGAC 
0361 CTCGTCCACG GAGGCTCAGC ACTACACCAA AGGCTGCTAC TGGTCCCCGG ACGGCACGTG 
0421 CCTTCTGGTG CCCGTTCACC TAGACGGCAT GCACGTTATA GAGATGCCCA GTGACCTCTA 
0481 CTCCGCAGAT ACGGTGCAGC  
 in silico PCR Fragment
0001 ATGCTGAATC ACAACGTGAG CTTCAGCAGC ACCATGGTCA CGTCCAATGG CGATCTTTCG 
0061 TTGCCCAAAC TGAGCGTGAC TCTTTCGGCG GAGGAGATCC TAAAGCTGCG TTCCGGGCGT 
0121 CCAAAGAACG CCGTTGAATC GCCGCATGCG GGTGTGCCCA TGGAGACAAG CCTGGCTGCC 
0181 GAGGAGGAAG CGAATGGGGA TGAGGAAGAG GAAAGCGTGC GGGTCGTCGA CATAGAGGAC 
0241 ACTTTGGAGC TGACGTCGAA TGGCAATGAG GCGAAGCCGG AAGACCAGGA GCTTGCGATT 
0301 GCTCCCGTCC TCCAGTACTT CCAGGGAGCA CTTGTTGAG- TTGGGCCGAC GCTGCTGGAC 
0361 CTCGTCCACG GAGGCTCAGC ACTACACCAA AGGCTGCTAC TGGTCCCCGG ACGGCACGTG 
0421 CCTTCTGGTG CCCGTTCACC TAGACGGCAT GCACGTTATA GAGATGCCCA GTGACCTCTA 
0481 CTCCGCAGAT ACGGTGCAGC  
 Assemble Data

9226R-1.IR_full       1   ATGCTGAATCACAACGTGAGCTTCAGCAGCACCATGGTCACGTCCAATGGCGATCTTTCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     1   ATGCTGAATCACAACGTGAGCTTCAGCAGCACCATGGTCACGTCCAATGGCGATCTTTCG 60


9226R-1.IR_full       61  TTGCCCAAACTGAGCGTGACTCTTTCGGCGGAGGAGATCCTAAAGCTGCGTTCCGGGCGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     61  TTGCCCAAACTGAGCGTGACTCTTTCGGCGGAGGAGATCCTAAAGCTGCGTTCCGGGCGT 120


9226R-1.IR_full       121 CCAAAGAACGCCGTTGAATCGCCGCATGCGGGTGTGCCCATGGAGACAAGCCTGGCTGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     121 CCAAAGAACGCCGTTGAATCGCCGCATGCGGGTGTGCCCATGGAGACAAGCCTGGCTGCC 180


9226R-1.IR_full       181 GAGGAGGAAGCGAATGGGGATGAGGAAGAGGAAAGCGTGCGGGTCGTCGACATAGAGGAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     181 GAGGAGGAAGCGAATGGGGATGAGGAAGAGGAAAGCGTGCGGGTCGTCGACATAGAGGAC 240


9226R-1.IR_full       241 ACTTTGGAGCTGACGTCGAATGGCAATGAGGCGAAGCCGGAAGACCAGGAGCTTGCGATT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     241 ACTTTGGAGCTGACGTCGAATGGCAATGAGGCGAAGCCGGAAGACCAGGAGCTTGCGATT 300


9226R-1.IR_full       301 GCTCCCGTCCTCCAGTACTTCCAGGGAGCACTTGTTGAGTTTGGGCCGACGCTGCTGGAC 360
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
9226R-1.in silico     301 GCTCCCGTCCTCCAGTACTTCCAGGGAGCACTTGTTGAG-TTGGGCCGACGCTGCTGGAC 360


9226R-1.IR_full       361 CTCGTCCACGGAGGCTCAGCACTACACCAAAGGCTGCTACTGGTCCCCGGACGGCACGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     361 CTCGTCCACGGAGGCTCAGCACTACACCAAAGGCTGCTACTGGTCCCCGGACGGCACGTG 420


9226R-1.IR_full       421 CCTTCTGGTGCCCGTTCACCTAGACGGCATGCACGTTATAGAGATGCCCAGTGACCTCTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9226R-1.in silico     421 CCTTCTGGTGCCCGTTCACCTAGACGGCATGCACGTTATAGAGATGCCCAGTGACCTCTA 480


9226R-1.IR_full       481 CTCCGCAGATACGGTGCAGC 500
                          ||||||||||||||||||||
9226R-1.in silico     481 CTCCGCAGATACGGTGCAGC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   481  NM_135153.2  CG9226-RA (CG9226), mRNA 
0   NM_140007.1  CG5747-RA (mfr), mRNA 
0   NM_137342.2  CG30460-RC, transcript variant C (CG30460), mRNA 
0   NM_165425.2  CG2682-RB, transcript variant B (d4), mRNA 
0   NM_165424.2  CG2682-RC, transcript variant C (d4), mRNA 
0   NM_136319.3  CG2682-RA, transcript variant A (d4), mRNA 
0   NM_079066.2  CG15110-RA (botv), mRNA 
0   12  NM_141446.1  CG10055-RA (CG10055), mRNA 
0   NM_141028.1  CG12977-RA (CG12977), mRNA 
0   NM_001014715.1  CG33513-RB, transcript variant B (Nmdar2), mRNA 
0   NM_001014714.1  CG33513-RC, transcript variant C (Nmdar2), mRNA 
0   NM_001014716.1  CG33513-RA, transcript variant A (Nmdar2), mRNA 
0   NM_141341.2  CG1208-RA (CG1208), mRNA 
0   NM_001043266.1  CG34119-RA (CG34119), mRNA 
0   11  NM_130639.2  CG2918-RA (CG2918), mRNA 
0   NM_176185.1  CG8421-RE, transcript variant E (Asph), mRNA 
0   NM_166141.2  CG8421-RD, transcript variant D (Asph), mRNA 
0   NM_166140.1  CG8421-RA, transcript variant A (Asph), mRNA 
0   NM_079033.2  CG8421-RB, transcript variant B (Asph), mRNA 
0   NM_137572.2  CG10062-RA (CG10062), mRNA 
0   NM_132457.1  CG11122-RA (CG11122), mRNA 
0   NM_137581.2  CG11242-RA (CG11242), mRNA 
0   NM_165889.1  CG30043-RA (CG30043), mRNA 
0   NM_001015134.1  CG40225-PA.3 (CG40225), mRNA 
0   NR_002021.1  CR33328, miscRNA 
0   NM_001042868.1  CG34124-RA (CG34124), mRNA 
0   NM_168016.1  CG17723-RC, transcript variant C (CG17723), mRNA 
0   NM_168017.1  CG17723-RD, transcript variant D (CG17723), mRNA 
0   NM_080248.1  CG6725-RA (Sulf1), mRNA 
0   NM_143242.2  CG6420-RA (CG6420), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
DC
DM
VM
VB
 
Dcompartment   Penetrance
     

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