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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9187R-3 
 Symbol Psf1  Full Name Psf1 
 CG No CG9187  Old CG No CG9187 
 Synonyms CG9187, Psf1 
 Accession No (Link to NCBI) NM_138242.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Kohzaki H.<br> The function of replication and SCF complex during Drosophila wing development.<br> Front Biosci (Landmark Ed) (2018) 23(12) 2235-2244 [ PubMed ID = 29772558 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTGCTAAAAGAACTGGAGAG 
 Primer Seq. 3' 
aatctagaggtaccGGCTGTTCTTCTTCAGGTGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTGCTAAAA GAACTGGAGA GATCGTCGCA GACAATTCCC GCCTTCGACG ACGATGGGGT 
0061 GCGCCAGGTG CTGGAGGAGA TCAAGGCGAT ATTCGAGGAG AACGTGGCCC AGGCCTCCAG 
0121 CTACAATGCT TCCGGGGATC GCAGTCTGTG GCCCCTGCTC AACTTCAGGC ACGCAGCTCT 
0181 CCAGAGGAAC AAGAGGTGCC TGCTGGCCTA CCTATACGAG CGGTGCAGGA GGATCAAGGC 
0241 CCTCAGGTGG GAGTTTGGAC CCATTATCCC CGGGGACATC AAGCAGGCAC TCTGCGAACC 
0301 GGAGGTCACC TTCTTCAACA ACTACTCCAA GTCCCTGGCT GCCTACATGT GCAGTGCGGG 
0361 CTACAACCAG GGCTTGCCCA TTGATCTAAC CAACAATCTG CGTCCGCCCA AGTCACTTTA 
0421 CATCGAGGTG CGCTGCATGG AGGACTATGG GAAGTTCGAG CTGGACGACG GCGAGGTGAT 
0481 CCACCTGAAG AAGAACAGCC  
 in silico PCR Fragment 
0001 CCTGCTAAAA GAACTGGAGA GATCGTCGCA GACAATTCCC GCCTTCGACG ACGATGGGGT 
0061 GCGCCAGGTG CTGGAGGAGA TCAAGGCGAT ATTCGAGGAG AACGTGGCCC AGGCCTCCAG 
0121 CTACAATGCT TCCGGGGATC GCAGTCTGTG GCCCCTGCTC AACTTCAGGC ACGCAGCTCT 
0181 CCAGAGGAAC AAGAGGTGCC TGCTGGCCTA CCTATACGAG CGGTGCAGGA GGATCAAGGC 
0241 CCTCAGGTGG GAGTTTGGAC CCATTATCCC CGGGGACATC AAGCAGGCAC TCTGCGAACC 
0301 GGAGGTCACC TTCTTCAACA ACTACTCCAA GTCCCTGGCT GCCTACATGT GCAGTGCGGG 
0361 CTACAACCAG GGCTTGCCCA TTGATCTAAC CAACAATCTG CGTCCGCCCA AGTCACTTTA 
0421 CATCGAGGTG CGCTGCATGG AGGACTATGG GAAGTTCGAG CTGGACGACG GCGAGGTGAT 
0481 CCACCTGAAG AAGAACAGCC  
 Assemble Data 
<PRE>

9187R-3.IR_full       1   CCTGCTAAAAGAACTGGAGAGATCGTCGCAGACAATTCCCGCCTTCGACGACGATGGGGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     1   CCTGCTAAAAGAACTGGAGAGATCGTCGCAGACAATTCCCGCCTTCGACGACGATGGGGT 60


9187R-3.IR_full       61  GCGCCAGGTGCTGGAGGAGATCAAGGCGATATTCGAGGAGAACGTGGCCCAGGCCTCCAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     61  GCGCCAGGTGCTGGAGGAGATCAAGGCGATATTCGAGGAGAACGTGGCCCAGGCCTCCAG 120


9187R-3.IR_full       121 CTACAATGCTTCCGGGGATCGCAGTCTGTGGCCCCTGCTCAACTTCAGGCACGCAGCTCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     121 CTACAATGCTTCCGGGGATCGCAGTCTGTGGCCCCTGCTCAACTTCAGGCACGCAGCTCT 180


9187R-3.IR_full       181 CCAGAGGAACAAGAGGTGCCTGCTGGCCTACCTATACGAGCGGTGCAGGAGGATCAAGGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     181 CCAGAGGAACAAGAGGTGCCTGCTGGCCTACCTATACGAGCGGTGCAGGAGGATCAAGGC 240


9187R-3.IR_full       241 CCTCAGGTGGGAGTTTGGACCCATTATCCCCGGGGACATCAAGCAGGCACTCTGCGAACC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     241 CCTCAGGTGGGAGTTTGGACCCATTATCCCCGGGGACATCAAGCAGGCACTCTGCGAACC 300


9187R-3.IR_full       301 GGAGGTCACCTTCTTCAACAACTACTCCAAGTCCCTGGCTGCCTACATGTGCAGTGCGGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     301 GGAGGTCACCTTCTTCAACAACTACTCCAAGTCCCTGGCTGCCTACATGTGCAGTGCGGG 360


9187R-3.IR_full       361 CTACAACCAGGGCTTGCCCATTGATCTAACCAACAATCTGCGTCCGCCCAAGTCACTTTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     361 CTACAACCAGGGCTTGCCCATTGATCTAACCAACAATCTGCGTCCGCCCAAGTCACTTTA 420


9187R-3.IR_full       421 CATCGAGGTGCGCTGCATGGAGGACTATGGGAAGTTCGAGCTGGACGACGGCGAGGTGAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9187R-3.in silico     421 CATCGAGGTGCGCTGCATGGAGGACTATGGGAAGTTCGAGCTGGACGACGGCGAGGTGAT 480


9187R-3.IR_full       481 CCACCTGAAGAAGAACAGCC 500
                          ||||||||||||||||||||
9187R-3.in silico     481 CCACCTGAAGAAGAACAGCC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_138242.1  CG9187-RA (Psf1), mRNA 
19.08   92  1  1  1  NM_206230.1  CG32318-RA (CG32318), mRNA 
0   0  3  2  6  NM_136386.2  CG3409-RA (CG3409), mRNA 
0   0  3  2  5  NM_170354.1  CG12428-RE, transcript variant E (CG12428), mRNA 
0   0  3  2  5  NM_170353.1  CG12428-RD, transcript variant D (CG12428), mRNA 
0   0  3  2  5  NM_170351.1  CG12428-RA, transcript variant A (CG12428), mRNA 
0   0  3  2  5  NM_143326.2  CG12428-RC, transcript variant C (CG12428), mRNA 
0   0  3  2  5  NM_170352.1  CG12428-RB, transcript variant B (CG12428), mRNA 
0   0  3  2  4  NM_137757.2  CG10318-RA, transcript variant A (NC2alpha), mRNA 
0   0  3  2  4  NM_166472.1  CG10318-RB, transcript variant B (NC2alpha), mRNA 
0   0  2  4  3  NM_169701.2  CG31045-RB, transcript variant B (Mhcl), mRNA 
0   0  2  4  3  NM_169700.2  CG31045-RA, transcript variant A (Mhcl), mRNA 
0   0  2  4  3  NM_001043250.1  CG31045-RG, transcript variant G (Mhcl), mRNA 
0   0  2  4  2  NM_079007.2  CG17716-RA (fas), mRNA 
0   0  2  4  2  NM_143807.2  CG3558-RA, transcript variant A (CG3558), mRNA 
0   0  2  4  2  NM_164519.1  CG3558-RB, transcript variant B (CG3558), mRNA 
0   0  2  3  2  NM_139767.1  CG13293-RA (CG13293), mRNA 
0   0  2  2  5  NM_135735.1  CG5525-RA (CG5525), mRNA 
0   0  1  6  4  NM_079460.2  CG18803-RA, transcript variant A (Psn), mRNA 
0   0  1  6  4  NM_168856.1  CG18803-RB, transcript variant B (Psn), mRNA 
0   0  1  4  4  NM_167358.1  CG18803-RB, transcript variant B (Psn), mRNA,4,5-triphosphate kinase 2 CG1630-RB, transcript variant B (IP3K2), mRNA 
0   0  1  4  4  NM_132647.1  CG18803-RB, transcript variant B (Psn), mRNA,4,5-triphosphate kinase 2 CG1630-RB, transcript variant B (IP3K2), mRNA,4,5-triphosphate kinase 2 CG1630-RA, transcript variant A (IP3K2), mRNA 
0   0  1  4  3  NM_001038781.1  CG33979-RB, transcript variant B (capt), mRNA 
0   0  1  3  8  NM_143497.2  CG7896-RA (CG7896), mRNA 
0   0  1  3  7  NM_167239.2  CG32677-RA (CG32677), mRNA 
0   0  1  3  5  NM_206235.1  CG13921-RB, transcript variant B (CG13921), mRNA 
0   0  1  3  5  NM_139399.1  CG13921-RA, transcript variant A (CG13921), mRNA 
0   0  1  3  4  NM_176110.1  CG2049-RB, transcript variant B (Pkn), mRNA 
0   0  1  3  4  NM_176111.1  CG2049-RC, transcript variant C (Pkn), mRNA 
0   0  1  3  4  NM_176112.1  CG2049-RF, transcript variant F (Pkn), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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