NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8836R-2

Symbol Cpr49Ad Full Name Cuticular protein 49Ad

CG No CG8836 Old CG No CG8836

Synonyms Cpr49Ad, CG8836

Accession No (Link to NCBI) NM_136929.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zool. Sci. (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGAAGCTGTTCTCCTGCCTTT

Primer Seq. 3'

aatctagaggtaccGTATAGACTACATTGGCAGGG

Predicted Fragment Size

IR fragment full Seq

0001 TGAAGCTGTT CTCCTGCCTT TTCGTGCTCA CCCTGGCTGC CATCGCCTGT GAGGGACAGC 
0061 CTCAATACAA TCCCTATCTG CGCAATCCCT ACTACAGGGA TCTCTACTAC AGGAACCGCG 
0121 ATCTCTACAA TCTACGACGT TTCTACGATG GCTTCTACAA GAAGTACAAT CCCTACATCG 
0181 CCGCTGCCCA GGCTAGAATT GTGGAGCAAA ACAATGATGT GAACTACGGA GCGGGCTCCT 
0241 ATTCCTACAA CTATGAGACG GAGAACGGCA TCCATGGCGA GGAGCGCGGA GTGCCAGTGA 
0301 ACATTGGAAA CCAGCAGCAA GAGGAGCAAG TTGAGGGTGC TTATTCGTTC ATTACACCCG 
0361 AGGGTCTGCG AGTGGGAGTC AAGTACCTGG CTGATGCCAA TGGTTTCCGT CCCGTGATCA 
0421 CCTATGATGG TGTGAACTCC GCTTTTTATG CCGGACAACC AGCCCCTGCC AATGTAGTCT 
0481 ATAC

in silico PCR Fragment

0001 TGAAGCTGTT CTCCTGCCTT TTCGTGCTCA CCCTGGCTGC CATCGCCTGT GAGGGACAGC 
0061 CTCAATACAA TCCCTATCTG CGCAATCCCT ACTACAGGGA TCTCTACTAC AGGAACCGCG 
0121 ATCTCTACAA TCTACGACGT TTCTACGATG GCTTCTACAA GAAGTACAAT CCCTACATCG 
0181 CCGCTGCCCA GGCTAGAATT GTGGAGCAAA ACAATGATGT GAACTACGGA GCGGGCTCCT 
0241 ATTCCTACAA CTATGAGACG GAGAACGGCA TCCATGGCGA GGAGCGCGGA GTGCCAGTGA 
0301 ACATTGGAAA CCAGCAGCAG GAGGAGCAAG TTGAGGGTGC TTATTCGTTC ATTACACCCG 
0361 AGGGTCTGCG AGTGGGAGTC AAGTACCTGG CTGATGCCAA TGGTTTCCGT CCCGTGATCA 
0421 CCTATGATGG TGTGAACTCC GCTTTTTATG CCGGACAGCC AGCCCCTGCC AATGTAGTCT 
0481 ATAC

Assemble Data


8836R-2.IR_full       1   TGAAGCTGTTCTCCTGCCTTTTCGTGCTCACCCTGGCTGCCATCGCCTGTGAGGGACAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     1   TGAAGCTGTTCTCCTGCCTTTTCGTGCTCACCCTGGCTGCCATCGCCTGTGAGGGACAGC 60


8836R-2.IR_full       61  CTCAATACAATCCCTATCTGCGCAATCCCTACTACAGGGATCTCTACTACAGGAACCGCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     61  CTCAATACAATCCCTATCTGCGCAATCCCTACTACAGGGATCTCTACTACAGGAACCGCG 120


8836R-2.IR_full       121 ATCTCTACAATCTACGACGTTTCTACGATGGCTTCTACAAGAAGTACAATCCCTACATCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     121 ATCTCTACAATCTACGACGTTTCTACGATGGCTTCTACAAGAAGTACAATCCCTACATCG 180


8836R-2.IR_full       181 CCGCTGCCCAGGCTAGAATTGTGGAGCAAAACAATGATGTGAACTACGGAGCGGGCTCCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     181 CCGCTGCCCAGGCTAGAATTGTGGAGCAAAACAATGATGTGAACTACGGAGCGGGCTCCT 240


8836R-2.IR_full       241 ATTCCTACAACTATGAGACGGAGAACGGCATCCATGGCGAGGAGCGCGGAGTGCCAGTGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     241 ATTCCTACAACTATGAGACGGAGAACGGCATCCATGGCGAGGAGCGCGGAGTGCCAGTGA 300


8836R-2.IR_full       301 ACATTGGAAACCAGCAGCAAGAGGAGCAAGTTGAGGGTGCTTATTCGTTCATTACACCCG 360
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     301 ACATTGGAAACCAGCAGCAGGAGGAGCAAGTTGAGGGTGCTTATTCGTTCATTACACCCG 360


8836R-2.IR_full       361 AGGGTCTGCGAGTGGGAGTCAAGTACCTGGCTGATGCCAATGGTTTCCGTCCCGTGATCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-2.in silico     361 AGGGTCTGCGAGTGGGAGTCAAGTACCTGGCTGATGCCAATGGTTTCCGTCCCGTGATCA 420


8836R-2.IR_full       421 CCTATGATGGTGTGAACTCCGCTTTTTATGCCGGACAACCAGCCCCTGCCAATGTAGTCT 480
                          ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8836R-2.in silico     421 CCTATGATGGTGTGAACTCCGCTTTTTATGCCGGACAGCCAGCCCCTGCCAATGTAGTCT 480


8836R-2.IR_full       481 ATAC 484
                          ||||
8836R-2.in silico     481 ATAC 484

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	466	0	0	0	NM_136929.1	CG8836-RA (CG8836), mRNA
0	0	3	4	3	NM_140786.1	CG18234-RB (CG18234), mRNA
0	0	2	4	3	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	2	3	22	NM_079902.2	CG6741-RB, transcript variant B (a), mRNA
0	0	2	3	22	NM_166514.1	CG6741-RA, transcript variant A (a), mRNA
0	0	2	3	22	NM_001014543.1	CG6741-RC, transcript variant C (a), mRNA
0	0	2	3	7	NM_078679.2	CG6816-RB, transcript variant B (Cyp18a1), mRNA
0	0	2	3	7	NM_080253.2	CG5067-RA (cic), mRNA
0	0	2	3	7	NM_167628.1	CG6816-RA, transcript variant A (Cyp18a1), mRNA
0	0	1	7	5	NM_137829.1	CG10384-RA (CG10384), mRNA
0	0	1	5	3	NM_138065.1	CG3419-RA, transcript variant A (CG3419), mRNA
0	0	1	5	3	NM_206215.1	CG3419-RB, transcript variant B (CG3419), mRNA
0	0	1	4	4	NM_136503.2	CG12769-RA, transcript variant A (CG12769), mRNA
0	0	1	4	4	NM_165585.1	CG12769-RB, transcript variant B (CG12769), mRNA
0	0	1	4	4	NM_137919.1	CG13545-RA (CG13545), mRNA
0	0	1	3	15	NM_134890.2	CG17265-RA (CG17265), mRNA
0	0	1	3	12	NM_166604.1	CG9812-RC, transcript variant C (CG9812), mRNA
0	0	1	3	7	NM_132017.1	CG3160-RA (CG3160), mRNA
0	0	1	3	4	NM_134576.2	CG1532-RA (CG1532), mRNA
0	0	1	3	4	NM_057249.4	CG7793-RA (Sos), mRNA
0	0	1	3	4	NM_169785.1	CG7223-RC, transcript variant C (htl), mRNA
0	0	1	3	4	NM_169784.1	CG7223-RA, transcript variant A (htl), mRNA
0	0	1	3	4	NM_176247.1	CG33143-RB, transcript variant B (CG33143), mRNA
0	0	1	3	4	NM_176246.1	CG33143-RC, transcript variant C (CG33143), mRNA
0	0	1	3	3	NM_139925.2	CG7979-RA (CG7979), mRNA
0	0	1	3	2	NM_142421.2	CG7218-RA (CG7218), mRNA
0	0	1	3	2	NM_132182.1	CG15326-RA (CG15326), mRNA
0	0	1	2	10	NM_141639.1	CG16779-RA (CG16779), mRNA
0	0	1	2	8	NM_132074.1	CG3585-RA (CG3585), mRNA
0	0	1	2	5	NM_001043247.1	CG6535-RB (tefu), mRNA

Back