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Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8785R-2 
 Symbol CG8785  Full Name CG8785 
 CG No CG8785  Old CG No CG8785 
 Synonyms CG8785 
 Accession No (Link to NCBI) NM_136960.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAAGATCAACTGGGATGTGCGT 
 Primer Seq. 3'
aatctagaggtaccAACTGCAGGCCAAAGGTGAAC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 AAGATCAACT GGGATGTGCG TATTTACATT GCCCTAACCG TGATCCCCTG TCTGCTTATC 
0061 GGTCAGATCC GTGACCTGAA GTGGCTGGTG CCaTTCTCCA TGATGGCCAA CATCTTCATT 
0121 GTGGTCACAT TCGCGATCAC GCTGTATTAC ATGTTCGACG AGCCGCTGGT TTACTCCGAC 
0181 AAGCCACTGA TCGCCAAGGC TGCCCATATT CCACTNCTTT TTCGCCACCG TGATCTTTGC 
0241 GATGGAGGGC ATCGGTGTTG TGATGCCCGT GGAGAACTCG ATGAGGAAGC CCCAGCATTT 
0301 CTTGGGATGc CCCGGCGTGC TTAACATTGC TATGGTCACT GTGGTCTCGC TCTATGCCAT 
0361 CATCGGATTC TTTGGATATG TGCGATTTGG CGACCAGGTG CGAGGCAGCA TCACGCTGAA 
0421 TCTgNCGGAA GGCGCCTGGC TGGGCGATAC gGNCAAGCTN CCTGATgGCC GTGGCCATCC 
0481 TGTTCACCTT TGGCCTGCAG TT 
 in silico PCR Fragment
0001 AAGATCAACT GGGATGTGCG TATTTACATT GCCCTAACCG TGATCCCCTG TCTGCTTATC 
0061 GGTCAGATCC GTGACCTGAA GTGGCTGGTG CCATTCTCCA TGATGGCCAA CATCTTCATT 
0121 GTGGTCACAT TCGCGATCAC GCTGTATTAC ATGTTCGACG AGCCGCTGGT TTACTCCGAC 
0181 AAGCCACTGA TCGCCAAGGC TGCCCATATT CCACT-CTTT TTCGCCACCG TGATCTTTGC 
0241 GATGGAGGGC ATCGGTGTTG TGATGCCCGT GGAAAACTCG ATGAGGAAGC CCCAGCATTT 
0301 CTTGGGATGC CCCGGCGTGC TTAACATTGC TATGGTCACT GTGGTCTCGC TCTATGCCAT 
0361 CATCGGATTC TTTGGATATG TGCGATTTGG CGACCAGGTG CGAGGCAGCA TCACGCTGAA 
0421 TCTGCCGGAA GGCGCCTGGC TGGGCGATAC GGCCAAGCT- CCTGATGGCC GTGGCCATCC 
0481 TGTTCACCTT TGGCCTGCAG TT 
 Assemble Data

8785R-2.IR_full       1   AAGATCAACTGGGATGTGCGTATTTACATTGCCCTAACCGTGATCCCCTGTCTGCTTATC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8785R-2.in silico     1   AAGATCAACTGGGATGTGCGTATTTACATTGCCCTAACCGTGATCCCCTGTCTGCTTATC 60


8785R-2.IR_full       61  GGTCAGATCCGTGACCTGAAGTGGCTGGTGCCATTCTCCATGATGGCCAACATCTTCATT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8785R-2.in silico     61  GGTCAGATCCGTGACCTGAAGTGGCTGGTGCCATTCTCCATGATGGCCAACATCTTCATT 120


8785R-2.IR_full       121 GTGGTCACATTCGCGATCACGCTGTATTACATGTTCGACGAGCCGCTGGTTTACTCCGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8785R-2.in silico     121 GTGGTCACATTCGCGATCACGCTGTATTACATGTTCGACGAGCCGCTGGTTTACTCCGAC 180


8785R-2.IR_full       181 AAGCCACTGATCGCCAAGGCTGCCCATATTCCACTNCTTTTTCGCCACCGTGATCTTTGC 240
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
8785R-2.in silico     181 AAGCCACTGATCGCCAAGGCTGCCCATATTCCACT-CTTTTTCGCCACCGTGATCTTTGC 240


8785R-2.IR_full       241 GATGGAGGGCATCGGTGTTGTGATGCCCGTGGAGAACTCGATGAGGAAGCCCCAGCATTT 300
                          ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
8785R-2.in silico     241 GATGGAGGGCATCGGTGTTGTGATGCCCGTGGAAAACTCGATGAGGAAGCCCCAGCATTT 300


8785R-2.IR_full       301 CTTGGGATGCCCCGGCGTGCTTAACATTGCTATGGTCACTGTGGTCTCGCTCTATGCCAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8785R-2.in silico     301 CTTGGGATGCCCCGGCGTGCTTAACATTGCTATGGTCACTGTGGTCTCGCTCTATGCCAT 360


8785R-2.IR_full       361 CATCGGATTCTTTGGATATGTGCGATTTGGCGACCAGGTGCGAGGCAGCATCACGCTGAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8785R-2.in silico     361 CATCGGATTCTTTGGATATGTGCGATTTGGCGACCAGGTGCGAGGCAGCATCACGCTGAA 420


8785R-2.IR_full       421 TCTGNCGGAAGGCGCCTGGCTGGGCGATACGGNCAAGCTNCCTGATGGCCGTGGCCATCC 480
                          |||| ||||||||||||||||||||||||||| |||||| ||||||||||||||||||||
8785R-2.in silico     421 TCTGCCGGAAGGCGCCTGGCTGGGCGATACGGCCAAGCT-CCTGATGGCCGTGGCCATCC 480


8785R-2.IR_full       481 TGTTCACCTTTGGCCTGCAGTT 502
                          ||||||||||||||||||||||
8785R-2.in silico     481 TGTTCACCTTTGGCCTGCAGTT 502

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136960.2  CG8785-RA, transcript variant A (CG8785), mRNA 
100   482  NM_165932.1  CG8785-RB, transcript variant B (CG8785), mRNA 
0.2   NM_137763.3  CG17922-RA (CG17922), mRNA 
0   30  26  NM_206319.1  CG6327-RD, transcript variant D (CG6327), mRNA 
0   30  26  NM_140167.1  CG6327-RA, transcript variant A (CG6327), mRNA 
0   30  26  NM_168431.1  CG6327-RB, transcript variant B (CG6327), mRNA 
0   30  26  NM_168432.1  CG6327-RC, transcript variant C (CG6327), mRNA 
0   NM_170542.1  CG18729-RA (zwilch), mRNA 
0   NM_080353.3  CG3422-RA (Pros28.1), mRNA 
0   19  NM_140070.2  CG3424-RA, transcript variant A (path), mRNA 
0   19  NM_168349.1  CG3424-RC, transcript variant C (path), mRNA 
0   NM_079770.2  CG7748-RA (OstStt3), mRNA 
0   NM_169955.2  CG10823-RB, transcript variant B (SIFR), mRNA 
0   NM_142709.2  CG10823-RA, transcript variant A (SIFR), mRNA 
0   NM_136522.1  CG14760-RA (CG14760), mRNA 
0   NM_206191.1  CG15669-RF, transcript variant F (MESK2), mRNA 
0   NM_166456.2  CG15669-RC, transcript variant C (MESK2), mRNA 
0   NM_166455.1  CG15669-RB, transcript variant B (MESK2), mRNA 
0   NM_206192.1  CG15669-RI, transcript variant I (MESK2), mRNA 
0   NM_079080.2  CG15669-RD, transcript variant D (MESK2), mRNA 
0   NM_206190.1  CG15669-RE, transcript variant E (MESK2), mRNA 
0   NM_206193.1  CG15669-RJ, transcript variant J (MESK2), mRNA 
0   NM_166454.1  CG15669-RA, transcript variant A (MESK2), mRNA 
0   NM_206194.1  CG15669-RK, transcript variant K (MESK2), mRNA 
0   NM_136640.2  CG8801-RA (CG8801), mRNA 
0   NM_079078.2  CG9707-RA (Acox57D-p), mRNA 
0   NM_134810.2  CG15385-RA (CG15385), mRNA 
0   NM_134285.1  CG6235-RC, transcript variant C (tws), mRNA 
0   NM_169330.1  CG6235-RG, transcript variant G (tws), mRNA 
0   NM_169331.1  CG6235-RH, transcript variant H (tws), mRNA 

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